LOCUS       sequence-458335-        9999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing the SSD1 allele from L-1374, under control of its
            native promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Paltenghi C, van Leeuwen J
  TITLE     Genetic suppression interactions are highly conserved across 
            genetically diverse yeast isolates.
  JOURNAL   G3 (Bethesda). 2025 Mar 3:jkaf047. doi: 10.1093/g3journal/jkaf047.
  PUBMED    40037589
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2025 Mar 3:jkaf047. doi: 10.1093/g3journal/jkaf047."
COMMENT     Sequence Label: pRS316-SSD1-L1374
FEATURES             Location/Qualifiers
     source          1..9999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6274..6293)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6275..6293)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6300..6317)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6300..6316)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6309..6331)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6457..6912
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6544..6563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6754..6775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7043..7846)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7847..8062)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8086..8105)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8205..8227
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8265..8283)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    8324..8827
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8864..8968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8969..9037,9038..9829)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9187..9206)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtaccggg ccccccccca gcgcataaaa agtaagcttt
     1081 cagtacttct ctttgatgat atttgtacag caaaagggta tttagtaacg gaataaattt
     1141 aatatataag agtataaata acaaaagaca atataaattg aaaagttgta aatattgaaa
     1201 agaaggctgc attattgttt tatgtatttt tgggaaaaaa tatatgactt aacgaggtcg
     1261 aataaaaaaa tttggatgat gccttaccgg aacgtggatt aaaaggagaa gtacaaaatt
     1321 catgcagttg ttaatgttcg caggaaataa aaacaaaggg aagggatact gaggggtgaa
     1381 gcacaatcca aagaacgaaa gtgaaaaaca agaaaaacag caatgacgat attggtagaa
     1441 gagattatac cctcttcatg aatggattta atgcacggac ggttaaacat ggcaaagcca
     1501 tccccacctc agctctcaat agaataggaa tcttttgcaa ttcatgtatt tcttgtatat
     1561 agttatcatt ttcaattctg gtttcagtag tagaaatgaa tttttctaaa gcattttgct
     1621 tgttgtcgct tgcagatggt agccttaaat ttggtactgt tagatgcaaa tcgctgagtt
     1681 ccttactcaa tggatcgctg atcaatggtt cagattccgc ttctttatct agttcaatat
     1741 tcgcaatctc agcggctgtg gatctgaatt tgttcttaat ggaatttcta taggattttg
     1801 gatttttttc atctgctggt ataaaagttg cactatctac gccgggctgc caatgcaatt
     1861 ccaagacacg attggtacca tcaaactcag ctttgatcaa aggtagttga tctccatgaa
     1921 ctctcttttc aataccaaat tctggaataa atacatcaaa ggaggactcg taaacttgta
     1981 agacagtagc cattgttaat aattgtcctg tagtatttcc catgtcgttg attgttttac
     2041 acaacaatag atgaattgct tgttcctgtg cttgataagc acagtccttt ttaaaattac
     2101 aatattcgga ggtaatcttc aaagcttcca tatcttcggt gtatggagta tcgtggataa
     2161 cggcctttaa ttgcctatga acgacatgat cagcgtatct tctcattggc gctgtgaaat
     2221 gtgtgtagat aggtaggttc aaggcataat gcccatattg gtccgggtct actttgccag
     2281 caataaagta tctagctctt ggcatggttt taaacagtaa aatttcaatt ccgactctaa
     2341 catcgtcatc tttaattttt agcacccctt tgattaattc atccgccgta ttggtatcaa
     2401 aattgtaacc aaaattttga attttctttc taaatgacgc catcttggtt gcaatgggtt
     2461 gcatctgcct tctcaaaaga gctagatcac caagtttggt gtaaattttc tctgcaacag
     2521 tggagttgac ctttctttta atctcattaa ttacaacaaa gcctaaagtt ctatccagga
     2581 tgttcaagtc aaccttaact ttttcgtcat ccaagctttc caataaggac agggtgggaa
     2641 gtaatgtagc ttctggatca tttattcttc tagcataaaa tgatctagca atttcctgta
     2701 cagtagagag gtagctagtg ggacttccag tagataattt ttcgtctaat tgttctaaag
     2761 acaagatgtt tgagggggaa attgtagatt cgcctaccca agtagattta atccttaaag
     2821 tagatgagtc tagagtgtaa acaaccgaca acgtggctga ttccttgcca ggggccaacg
     2881 acagttcgtc gttgaacgat tgtggtaata aattgacaag tttttgtggc atgaataccg
     2941 cagaggacct ctttctcgca cgcctatcaa cagaggagcc ttcttcaata tggctggtca
     3001 catcaacaac atgacaaccc agctctaggg taccatttcc gttattcctg acatgtaagg
     3061 caaattcaga cacccatcca agctcggaaa ttgcaaagat attgtactca ttagtgtccg
     3121 taaaattcct cctatattct agactttcag ccgttaatgg tagcggctga aaacttggtt
     3181 tttctttttg cggatttttt tgatccaaat attcattcga aagaaaattg ttatccctta
     3241 aaatggaatc aatttcagta tcaggatcgt gaatatctcc aagttcggaa actaaaatac
     3301 caaatggatg caaagatgtg attggccaac gtttaataga ggcaacgaat aacttttcgg
     3361 agtatttatc agcgttttca acaaagtcct ttggagctaa ttctgtagga attgcaatta
     3421 atggcacctt cttatcagta ggcttgaacc aagcaatttt tgggctttgt ggtggtttgt
     3481 tattgtcgct attagcttgt tgggatggtc tcaacaaacc taatgtaccg ctaaataact
     3541 gaccagggat acggtccaaa acagcaacga catggcctgc gtaaagtggc ttatatttat
     3601 cgttgatttc ttcttcttca actaataaca atgactgacc ttcaacttca acatcgtcat
     3661 ttttcttttg agttggacgt tgtttcaatg aacttcttct ccttactcct gatttggtag
     3721 gagatgataa ggaatcacta tcattattga tagtagatga cctctttctt ctgacggata
     3781 aatcatcctt gcttagcgaa tcagacgagg agggagaaga tagaaaattg ttgcttgttg
     3841 cagcagtaac agatgcatcg ttgtggtaat cgtcactact gttcaaagga attagatcgt
     3901 gttgcataga ggcatccttt ctcctcttct tttcttcctt ttctttcttg gactcccaaa
     3961 catcgtccac aactaatagt tctaccgcga ctaaatcacc ttcaagtgct ctattacgat
     4021 ctttggagcc gcaaatgtaa atatccgcat caagagcgcc atctgtagag acccaggcat
     4081 ccgatctatt ctttttatta actcttaata taccagctac tagtctccct tcttggatta
     4141 gctctggaat gttggcttgg ggaaggtagg gtgcaaatag ggacttacgt ccacctccgt
     4201 tattattacc ttgtgaactg aaactatgaa ctgatgaatt agaggcacga tgtttgtgtc
     4261 cctgctgaaa tatcgcagga ggttctaagg tattgaagga attgtaatcc cttgaattag
     4321 atcctctgtg gcggaatgga gaaagctgct gttgaggttg ctgtgattgg tttctccagt
     4381 tggaattaat gcctggagaa tattcattgt ttcttgtctt tgaacggcgg tgcgttgaag
     4441 aggttgctat gaattcatcg tcattatccc cgtgagagtt tggaggaaat ttgaaagagg
     4501 gaggtgatag gtttgaagtt gcgcgacgat ggccctgatg agcatttggt ggtggtggaa
     4561 atttaaaatc agattgttct gtagtagaat ttgagccact atcagcttga acagaatcta
     4621 tcttcacagt tacgcctgct tcacccccag atattctttt agcttgttct tccgcagcag
     4681 cctttttcgc ttcatttaga cctaaagaat gtcttctgga atggccatat atgctggatt
     4741 gtgaactcgt ctttctgtgt gagttagacc caggattatt gctattgtta ttgttatcat
     4801 atgaattatg ataatatccg gagttgttcc tggagtgagt tctacttctg ctgccacctg
     4861 cactcatacc aggtgaatta ccgtttgagt tgtaatgagg gtgaggcggt tgaggcgtga
     4921 aatgattgtt agagccagaa tttcctgaca ggtattgtcc tgtctgttgt gccaattgct
     4981 gttgctgagc catcaactgt tgctgctgtg cttgaacttg ctccaactgc ttctgcaaag
     5041 tgaattgttc aatcatcaaa tttgtcaact cactttggga acgtctgtgt gcaacatgaa
     5101 tctgctttgg ggcgttttta ccacctcctg tggtttgcac aaacatgtta tttggctctt
     5161 gggatctatt gttgttaacg ttgctatttt tagacatagt gtataaagat ggaataattg
     5221 ggccaaatct ttgcgctgaa aggtttttgg tgttctgttt tgcgatatta aagtgacaaa
     5281 gaaaaagatg gaacttggca aatactatac gtttatgaac ccttgtctta ttcttcgtgc
     5341 aggcaactta gatttccctt tgaaaaaaag ttgtttcttt tagaatagtg gctatgaaaa
     5401 aagtgcgcct aatgcggaaa aacgaggcac cagctgttct aaaataagac caaaatgttt
     5461 agggtaccag aaaaatggta tggcgaaaat aagcctggca agaagactat gcagataact
     5521 ttgactattc cggtttttag gttaccaaag acagaagaaa ggatcggtcc caaaaaaagg
     5581 aaatagctta taagggaagt tgcagaaaat gcgcttgagt aacaaaaaat gttagaacgt
     5641 agcaaatatt ctaaaaggaa tgacagaaaa acttcttacc tttctaaaat ataatctctc
     5701 ctatttctca gctaggctca aaattataat ttagaaatta ttgtttgcac ggcaaaactt
     5761 cgttcctttg ctgttagaaa atcagtcggt gttggactct tcgtctttta aattcttgac
     5821 aagagaagag tttttaaatg aaaagaacta acgagaaaaa accaggggaa ggagggaaag
     5881 agcgaaaata ctcgtgatct aaactctttt aggttagttt tcgttggagg cagtggtctt
     5941 aagacgatgg tgaaagaaag gacttatttg tagaaggatt gtttatttaa tccttgaact
     6001 aaacaaatga caaactcagg tctctagcgt tcctgttcgt ggcctttaat aacttgatgg
     6061 caatggtaga aagacacacc tgcgttatat aagtttagtt ttcccttctt ttcctttctc
     6121 atcccctcgt gaagcgactc ggcccctttg gtcagaaaat tcaaggtgat acaagtcgtc
     6181 gtccgtgaca taggatcagg aatcacaatc atcacattgc actctcgttc actagttcta
     6241 gagcggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta ttacaattca
     6301 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6361 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6421 ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa
     6481 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6541 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6601 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6661 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6721 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6781 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6841 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6901 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     6961 catagggtaa taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg
     7021 catttactta taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc
     7081 agcctgcttt tctgtaacgt tcaccctcta ccttagcatc ccttcccttt gcaaatagtc
     7141 ctcttccaac aataataatg tcagatcctg tagagaccac atcatccacg gttctatact
     7201 gttgacccaa tgcgtctccc ttgtcatcta aacccacacc gggtgtcata atcaaccaat
     7261 cgtaaccttc atctcttcca cccatgtctc tttgagcaat aaagccgata acaaaatctt
     7321 tgtcgctctt cgcaatgtca acagtaccct tagtatattc tccagtagat agggagccct
     7381 tgcatgacaa ttctgctaac atcaaaaggc ctctaggttc ctttgttact tcttctgccg
     7441 cctgcttcaa accgctaaca atacctgggc ccaccacacc gtgtgcattc gtaatgtctg
     7501 cccattctgc tattctgtat acacccgcag agtactgcaa tttgactgta ttaccaatgt
     7561 cagcaaattt tctgtcttcg aagagtaaaa aattgtactt ggcggataat gcctttagcg
     7621 gcttaactgt gccctccatg gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac
     7681 aaattttggg acctaatgct tcaactaact ccagtaattc cttggtggta cgaacatcca
     7741 atgaagcaca caagtttgtt tgcttttcgt gcatgatatt aaatagcttg gcagcaacag
     7801 gactaggatg agtagcagca cgttccttat atgtagcttt cgacatgatt tatcttcgtt
     7861 tcctgcaggt ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt
     7921 caacactaca tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct
     7981 tctgttcgga gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat
     8041 aaaaaaaaaa tgatgaattg aaaaggtggt atggtgcact ctcagtacaa tctgctctga
     8101 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     8161 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     8221 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     8281 atttttatag gttaatgtca tgataataat ggtttcttag acggatcgct tgcctgtaac
     8341 ttacacgcgc ctcgtatctt ttaatgatgg aataatttgg gaatttactc tgtgtttatt
     8401 tatttttatg ttttgtattt ggattttaga aagtaaataa agaaggtaga agagttacgg
     8461 aatgaagaaa aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat
     8521 atatatttat tagacaagaa aagcagatta aatagatata cattcgatta acgataagta
     8581 aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc
     8641 ggcattaata cctgagagca ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc
     8701 ctagagtctt ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac
     8761 tttctatttt taatttatat atttatatta aaaaatttaa attataatta tttttatagc
     8821 acgtgatgaa aaggacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8881 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8941 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9001 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9061 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9121 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9181 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9241 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     9301 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9361 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     9421 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9481 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9541 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9601 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9661 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9721 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9781 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9841 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9901 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9961 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
