LOCUS       sequence-458341-       12729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-11-LRRK2 G2019S in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12729)
  AUTHORS   Raig ND, Surridge KJ, Sanz-Murillo M, Dederer V, Kramer A, Schwalm 
            MP, Elson L, Chatterjee D, Mathea S, Hanke T, Leschziner AE, 
            Reck-Peterson SL, Knapp S
  TITLE     Type-II kinase inhibitors that target Parkinson's Disease-associated
            LRRK2.
  JOURNAL   bioRxiv [Preprint]. 2025 Feb 13:2024.09.17.613365. doi: 
            10.1101/2024.09.17.613365.
  PUBMED    39554022
REFERENCE   2  (bases 1 to 12729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Feb 13:2024.09.17.613365. doi: 
            10.1101/2024.09.17.613365."
COMMENT     Sequence Label: pcDNA5 FRT TO GFP11-LRRK2_G2019S
FEATURES             Location/Qualifiers
     source          1..12729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..2313)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(1291..2313)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(2321..2368)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     2500..2519
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2652..2876
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2865..2882
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(10517..10564)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     primer_bind     complement(10670..10694)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    10704..10722
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10725..10743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(10745..10948)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10774..10794)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10949..11328
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     11500..11519
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11594..11698
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11699..11767,11768..12559)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11917..11936)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagtacgtg ctattccttt gccctcggac gagtgctggg
     1321 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     1381 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     1441 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     1501 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     1561 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     1621 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     1681 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     1741 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     1801 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     1861 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     1921 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     1981 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     2041 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     2101 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     2161 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     2221 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     2281 ctcgacagac gtcgcggtga gttcaggctt tttggccaag gaagttccta tactttctag
     2341 agaataggaa cttcggaata ggaacttcta ggtacgtgaa ccatcaccct aatcaagttt
     2401 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     2461 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     2521 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     2581 gcttaatgcg ccgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc
     2641 cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
     2701 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
     2761 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
     2821 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga
     2881 ggctgatcag cgggtttaaa cgggccctct agactcgagc ggccgcttac tcaacagatg
     2941 ttcgtctcat tttttcagct aattcttttc tcacttcaat gtgtttttct aaattttgca
     3001 cttcatgtgg aagattgatg tcccaaacgg tcaagcaaga ttgtatctct ttctgctttt
     3061 gtgtaccttc agtatttttc cggttgtagc ccaataccag catgacattt ttaaggcttc
     3121 ctagctgtgc tgtcatcatg actctgaccg aattacaaaa gttgtaaatt acacgtataa
     3181 gtcgacgagt tgaaagatcc aggagtaaaa tatggcctcc tccagttcct atccaaagag
     3241 cagtgttctt ctgaaggcag agggttttca ctctcccaga ataagacatt ttgtgttttg
     3301 attccttgtt ttcttttacc attacctccc ttaaaaagtg cacgcagtct attagtccac
     3361 agagtttttc agttttctta tcccacactt ccacaacagg gctattttgc ttagcaatat
     3421 agagagcagt gtctaccacc actgttatga tgttggaatc actgaaagct gcataagaaa
     3481 acagttggct tgttcttgtc tcaatgagtt tctgaatggt gaaatcatta gaaaaggaga
     3541 aaatctttgt gccacatcct ccccacatta catttctttc cgttgaattt gtggattcac
     3601 tcaaacacat caatggagta ctgacatttc ctatatttag tatcttcaaa ggagcagctc
     3661 ctttaagctt aacagtctta tcttcaaaaa ttgctaactt gccatcagcg gttccaacca
     3721 aaagaaaatt tttttgtttg ctttgcttgg aaaaggaatt gcaatacaaa caagtgacag
     3781 aatcagtcat cttttctagg gtatgtctct ttttcccatc ttcggtattg atgaccagga
     3841 gagtaccaga ctgtgtccca gacacaatcc agctttcctt ttcaacagga agatgcacca
     3901 aggctaagca caatattcta ctatcagcaa cttcctcaga agtgtatcct tcagtattta
     3961 agtcaagaaa tgagagctgt cctctgtcgg tgtgcccaca gcccagccaa atgcttgcat
     4021 tcctgctgtt gtgatgtgta gcaaccatgc attcaacaat tacgttttta ggtaataaaa
     4081 tgcgtctcgt cagacagact aattcagctg aattcaaaat gtcaaagacc tgggcagaag
     4141 taggcctttc ttgaggattt tctttcaaac actgtttaat taatttctca accataggcc
     4201 atggggcaca accatattct ttaactggat caggtaattt tccttgtatt tctaattcat
     4261 caaactcatt tggaaacttc aaaccctcta ctattctacc tccagttgtc aaaatgtcat
     4321 agagtagtaa accaaatgaa taaacatcag cctgttggtt ataaatgaca tttcctctgg
     4381 caacttcagg tgcacgaaac cctggtgtgc cctctgatgt ttttatcccc attctacagc
     4441 agtactgagc aatgctgtag tcagcaatct ttgcgatgat ggcagcattg ggatacagtg
     4501 tgaaaagcag cacattgtgg ggtttcaggt ctcggtatat aatcatggct gagtggaggt
     4561 atctcaaacc atcagctacg tggagtgcga tcctgtgctg tagggttcta gtgaggctgg
     4621 ctttgtcctg ctgaagcagg cgatccaagg aacccttgga ggctaactcc atcaccaaca
     4681 tccggggacg aatcccagct gccagcaaag atatcaaact ggggtggtgg aggtggcaaa
     4741 gcaccacaag ctcttgtctt aacagcctga gtgatgtatg tttattaaaa atcttcacag
     4801 ccacttcttc tccttcatag gctgctcggt aaactgatcc aaaactgcca tcacctagga
     4861 gaaactctgg agcttgttca aattccaact catcattatt caacataata tttctaggca
     4921 ggtcagccaa aatcaagtca ggggcaatct gagatattgg aatggtgagc cttggttgat
     4981 ctggatttac taagagatct ccttcctctg ctttcttcat caagtcatca agtaagattt
     5041 tttgatgttc ttcgccatca ttaaaactat ataatgccca tttcttcaac agagtttctc
     5101 cttcaccaca aatatcaatc tccagcaacc caggaaacca ttcttccatg agagaatcaa
     5161 tgtggtccac aacttggccc aaaagaatac agccttttct acaagaagga actgtaattt
     5221 ttaagaaact ctctggatga ttgtctaaga cttcagatcc taccagacaa taagcttcag
     5281 gagaccaatt taagtaaatg ccttgtcgcc aatacattct gtttgggcga agtgctcgtt
     5341 ctctccctga aagcatgtaa ggtgaaatct caagtaatcg attgattaat cttgaccaaa
     5401 atcccattgg aaaataaggc atttcatata gtcggatgat aatttcagag ttctcacaat
     5461 ggggaagctc tatcacaggc ctgtggtcag acaaactgct tggaaccagc aaatattctt
     5521 ctcctattgg caaagcaatc tggaattttt ctaggagctt aaaatactgt gacatgtagt
     5581 tctttggaaa tttccttttt tttgaaagaa atttttccac atctctacgc gaaataattc
     5641 ccttagggtg ttttggacaa ccttccactt tcactgtcaa aatctgtgcc atgattttac
     5701 aaagccactt gggttccaca aagtacaagt cacttaactg cagtgctggg tcttgaaaat
     5761 gaagaaggac tcctgattca tttagaaagt gaactgcgtg aggaagctca ttttcatcta
     5821 actgcagctg attttctctc actagttgta ataatcgttt ccggtcaatt acgggaaatt
     5881 caattggcac atttttacgc tccgataaaa tgattttttc aagttctaca tagcagtctg
     5941 gaatcagctg tccaacaaca agctgatctc ggatcttgaa attaaggctc tcgtttatga
     6001 tggttttccg aagttttgcc aaagcatcag attcctcggt ggcattcaca aagtggtaat
     6061 ctcgtatggc agggaaccct cgcttattca ggagttcctt ggtgatttta ctcatgcagg
     6121 ctttgcgttg cttctcatca gaaacatcca aatgtgtgcc aacgagaatc acaggggaag
     6181 aagaagcgcg agcctttata ttgaagagcc aaggcttcat ggcatcaact tcagcctgtc
     6241 ccttgctgag gtcatagaca gcaaggtaca atgctcgctg cgtcataaaa tggggatgag
     6301 tactatagaa ttcctcacga cctgcaaaat cccacacatt taggacgaga tctctctttc
     6361 ttttgtctct tatttggata ggccagtctt tcacatctat gccaactgtg gcactttgca
     6421 ttccaagatc tgatttcttg gttttcatta attgctgcaa taaggtggtt ttaccactcc
     6481 cagtatttcc cacaatcata agtttcattc ggttataagg cacagccttt tttaatcgct
     6541 gttgaagaaa ccttatgatg tctttggctt tacatcctat atgtttaaaa tcaaagttaa
     6601 gatgcagttc atccaaagga agatcccata ttttgcttaa tttccccatt tcattgggaa
     6661 aggatcttag ttccaagttg taactgacat ccagagatgt cagattttca agacagccaa
     6721 tctcaggagg aatctctttc agtttattgt gagaaagatg cagtttctct actctagacc
     6781 ataaatatgc tttttcactc aagtccaaga tgctgatctg attatggcta aataagagtt
     6841 cccttaagtt caaagatttc cagtgtgcgg gacctggtag gtactgaata tcattgctgc
     6901 tcatatctaa agaccgcaag tgtggaagat ttaaaattgc ttctggaata caggaaaatt
     6961 tgttctgaga taattttagg attgtcatag aaggaggcaa gaaaggcata gcagcaagaa
     7021 aattcattct ggcactgaaa ctctccactt taggacaagc ctcaagaaag ttctctgata
     7081 gggatgaaat gtggttctta ctaaggttta aaatcttcag ttccttcagt ctcaaggggg
     7141 agcatatccc tgatatttta tttccttcta aaatgagctg ctccagtttc tctaccacat
     7201 cagtgaggtt ctcaggtaca aaagacagct ggttatatga caggttaaac tgtttcagag
     7261 ttggacattt cactgtagga tctaaaacca ctgagggtcc aatgtcattt cgagagacat
     7321 caagattagc aatacaactc attttcaaca aataagaagg aaatgatgta aatttattac
     7381 tgtgcaagtc caaatgtgtc aaactcttca gagtttcaca tagctgttgt ggaaagctcg
     7441 tgagtgcatt ctggtgaagc tccagctttt caagatgctc caaatgaaca cttatacagc
     7501 atttctggct tagggcatca atatctctta gttcatttgc tgaaaggtct agtgatgtaa
     7561 tatattctct ctcagaagcc agagaagaaa tgctgtctga atgcctcata tgggattgaa
     7621 gttttgatga cctgagtgaa tcatctgaag ataatatttt tctttttcgc ttcagtaaat
     7681 cttcatgatc aaaaatgggc cccaaggaat tggaatgtct ttgcaaattt ggtgagcaac
     7741 gctgtaatac ggcatctcgg taaaattctc ctacactaat tgaattagat ttctttttca
     7801 caagaaatga gccttcactt ccttcactat ccaggtcatc actttgagca aacacactgt
     7861 ccatagaaga gtcaggaata aaggtccatt catcaaattt agacagcaca tcttcagaaa
     7921 aatttccatc gctgcctgag gctgttcctt cttccacagc acttttcatc tgatatctga
     7981 tcaccattct tgctagtgta gatgctatat ttgtttgttt ccttaaatta gaagtcttat
     8041 ctggaaataa aggaccaagc caagaaggtt caacttttcc tatacaaaat cctccaaggc
     8101 aaatgctatt gttggcaaca tccagggcca gcctccttaa gagcaagctg atgatctggc
     8161 tgtcaccttt cccaatgctt atcgtcaacg cttttcgtac atcttgttca cgagatccac
     8221 tattcagtaa gagttccacc aatttgggac tgctctcttt ctcacatacc tgacaaatta
     8281 aagaagatcc ctcctttgct tgattggcat ctgctcccaa tagaagcaag cattcaacca
     8341 tgatgctgtt attctgatca cacgctctct ctagcatcac attttttaag taatcatcca
     8401 tagctacttt tgcaaaacac ttgcaacaga ggtttagaaa ctgttgatcc ttttgttcca
     8461 tgatattgga agacatttga tggaatatta ctaagtcaaa tgaatgatgc accagcagct
     8521 tagaaaaaga tgctgacaat ttgaggattg ctaagattgt ctgaaatcct ttagtctgta
     8581 tttcagcaac atccttaaat cggtataagc tggaaaccag aatttttgcc agcagatgtc
     8641 cagttcctat gaacacattc ttctttgtaa tcaagtatcc tataagactt aaacccagac
     8701 actgaatttc ttggtcatct ggatacatct gcagtgtgtg aagcactgaa tccatagcac
     8761 cttccaggga taacatctct aatgcatcag gaaaatgtac aatagaagaa attactttta
     8821 atccacattt ctgaatccca ggatttccaa tgaacctgtt caaagctgct aggaccagtt
     8881 tgtgaatatc attcttgaaa cactgttttt taaccatatt tagcttatga tgaaattctg
     8941 tatcctccct ggattcttct ggcatgccag gcactataaa atgtaaaata gctcgaagcg
     9001 cctccagctg cactggtaat gatgtctcat gacgtttcat aactgttagt attttgggga
     9061 ccactgctgc cattatatcc agggaagtgt tgcttccttc aaaaagatga tttagcattt
     9121 tacagccact ttcagccact tcgggagaat gtatatgctt ctgcattaac tccaaaacat
     9181 tcaggtgtat tccttttgat aacagtattt ttctgaaatt aacattttgt tctaagagag
     9241 ttgacaatgc attcgcagat gcctggaaaa cttcctttga tgaagaatgc atcagcatgg
     9301 agagcatcac ttccctatga gctgggaaat ggccatcttc atctccaatc ttctcatgta
     9361 aactgttttg gtacataagg agattattta gtgcccagca tgcggcctcc tgcacgtgct
     9421 tgttctttct atgccacgtt aatgctttgt aacaggcttc cagccaaaac aatttatctt
     9481 cttccccctc atcatcattc tcttgattct cattcttttc ctctaaatct tgatttaaga
     9541 aaatagtctc agtgaggagg gccaaacagc tgagcgctga gatctgcaat gctgcattct
     9601 ctgggtactg ctgcacagct ttcaccacaa actcatggac ttcgtttaat accaggatat
     9661 tgaaaaaatt acctaatgta agcctatgga gcaaacagca actcacttct tgaattcttt
     9721 cactcatagg gaatgctttc atagcttcca ccacaatatt ataacacctg acattgccac
     9781 tcatgaggac ttccacatta ttgcaaggaa tcgctaggga atgtaaacaa tgcagcacat
     9841 gaagcacaat ttcctcttca tctttaaaat ttgttaacgc acttaacaat atcatataat
     9901 ctttgttctc aacaaattca gtcagttgct cctctgagac tctctcaaac agcacatgta
     9961 aagctttgca tccaagtttc tggacttcat cattggctgg aaatgagtgc atggcatcaa
    10021 aaattaacat gaaaatatca ctttcttcat ccaatatcag caaggtgatt ttacctgaag
    10081 ttaggaggag atctaaggtc ttcagtccaa tcactgacaa gtttacactg gcattatgaa
    10141 ctgttagcat tttaagaatc aattggtgaa caccaaggac ttcccaatca tttccaacat
    10201 cctggggtcc cattaagctt tgcattgtac ctggacagac ttctattaat ttgcacagaa
    10261 gtgaccaacc cacctgctgc acactcgcga ctctcatata ggagtccaag acgatcaaca
    10321 gaggcacatg gatatttttg ccttgaaata acttggaggc gtgctcggag tacgtgaaca
    10381 ccagcagatc ctccaggatt tggaccagcg tttctatctg ttttccttcc tggacattgt
    10441 tcagcctgac tatcaacttc ttcagagttt cctcgtcctc ttcgcacccc tgacagctgc
    10501 cactagccat ggatcctgtg atgccggcgg cgttcacgta ctcgtgcagc accatgtggt
    10561 cccggctgcc catggtacca agcttaagtt taaacgctag agtccggagg ctggatcggt
    10621 cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt
    10681 tcactaaacg agctcgtcga cgatctctat cactgatagg gagatctcta tcactgatag
    10741 ggagagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
    10801 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
    10861 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
    10921 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
    10981 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
    11041 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
    11101 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
    11161 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
    11221 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
    11281 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc
    11341 tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat
    11401 tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct
    11461 ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac
    11521 tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag gtggcacttt
    11581 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    11641 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    11701 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    11761 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    11821 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    11881 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    11941 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    12001 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    12061 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    12121 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    12181 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    12241 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    12301 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    12361 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    12421 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    12481 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    12541 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    12601 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    12661 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    12721 aggatcttc
//
