LOCUS       sequence-458357-       11240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the 
            AAVdual system, which integrates the mini-pHelper-1.0 (providing 
            E2A, E4orf6, and VA RNA) with an AAV expression cassette containing 
            eGFP driven by the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Yang R, Tran NT, Chen T, Cui M, Wang Y, Sharma T, Liu Y, Zhang J, 
            Yuan X, Zhang D, Chen C, Shi Z, Wang L, Dai Y, Zaidi H, Liang J, 
            Chen M, Jaijyan D, Hu H, Wang B, Xu C, Hu W, Gao G, Yu D, Tai PWL, 
            Wang Q
  TITLE     AAVone: A cost-effective, single-plasmid solution for efficient AAV 
            production with reduced DNA impurities.
  JOURNAL   Mol Ther Nucleic Acids. 2025 May 14;36(2):102563. doi: 
            10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10.
  PUBMED    40503174
REFERENCE   2  (bases 1 to 11240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2025-05-14"; volume: "36(2):102563. doi"; 
            pages: " 10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10"
COMMENT     Sequence Label: pAAVdual-CMV-eGFP
FEATURES             Location/Qualifiers
     source          1..11240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     polyA_signal    170..646
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     428..447
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     misc_feature    678..1266
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             784..795
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1193..1213
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(1291..2004,2005..2007,2008..2010))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1294..2004,2005..2007,2008..2010))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1336..1357)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1685..1704
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1944..1965
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2007..2016
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          2021..2117
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(2147..2167)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2322..2700
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      8964..9418
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9050..9069)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9260..9281
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9444..9548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9549..10343
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9549..10343
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9603..9622)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10213..10232
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      10514..11102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11003..11022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgctc ggtccgcacg tggttaccta caaaatcaga aggacaggga
      181 agggagcagt ggttcacgcc tgtaatccca gcaatttggg aggccaaggt gggtagatca
      241 cctgagatta ggagttggag accagcctgg ccaatatggt gaaaccccgt ctctaccaaa
      301 aaaacaaaaa ttagctgagc ctggtcatgc atgcctggaa tcccaacaac tcgggaggct
      361 gaggcaggag aatcgcttga acccaggagg cggagattgc agtgagccaa gattgtgcca
      421 ctgcactcca gcttggttcc caatagaccc cgcaggccct acaggttgtc ttcccaactt
      481 gccccttgct ccataccacc cccctccacc ccataatatt atagaaggac acctagtcag
      541 acaaaatgat gcaacttaat tttattagga caaggctggt gggcactgga gtggcaactt
      601 ccagggccag gagaggcact ggggaggggt cacagggatg ccacccgtag atctctcgag
      661 cagcgctcgg tatcgatgcg gggaggcggc ccaaagggag atccgactcg tctgagggcg
      721 aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc
      781 tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac
      841 ataggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg aattgtcagt
      901 gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt
      961 ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc
     1021 ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac
     1081 gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa
     1141 agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt
     1201 aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt aatccagagg
     1261 ttgattatcg ataagcttga tatccgtacg ttacttgtac agctcgtcca tgccgagagt
     1321 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
     1381 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
     1441 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
     1501 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
     1561 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
     1621 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
     1681 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
     1741 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
     1801 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
     1861 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
     1921 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
     1981 ggtgaacagc tcctcgccct tgctcaccat ggtggcgcgc ctagaagtaa aggcaacatc
     2041 cactgaggag cagttctttg atttgcacca ccaccggatc cgggacctga aataaaagac
     2101 aaaaagacta aacttacgca ggtgcttata tagacctccc accgtacacg cctaccgccc
     2161 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
     2221 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
     2281 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
     2341 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     2401 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     2461 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     2521 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     2581 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     2641 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
     2701 acgcgtgcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     2761 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggcggcc tcagtgagcg
     2821 agcgagcgcg cagctgccaa acgtcgacgt aatccgtaga tgtacctgga catccaggtg
     2881 atgccggcgg cggtggtgga ggcgcgcgga aagtcgcgga cgcggttcca gatgttgcgc
     2941 agcggcaaaa agtgctccat ggtcgggacg ctctggccgg tgaggcgtgc gcagtcgttg
     3001 acgctctaga ccgtgcaaaa ggagagcctg taagcgggca ctcttccgtg gtctggtgga
     3061 taaattcgca agggtatcat ggcggacgac cggggttcga accccggatc cggccgtccg
     3121 ccgtgatcca tgcggttacc gcccgcgtgt cgaacccagg tgtgcgacgt cagacaacgg
     3181 gggagcgctc cttttggctt ccttccaggc gcggcggctg ctgcgctagc ttttttggcc
     3241 actggccgcg cgcggcgtaa gcggttaggc tggaaagcga aagcattaag tggctcgctc
     3301 cctgtagccg gagggttatt ttccaagggt tgagtcgcag gacccccggt tcgagtctcg
     3361 ggccggccgg actgcggcga acgggggttt gcctccccgt catgcaagac cccgcttgca
     3421 aattcctccg gaaacaggga cgagcccctt ttttgctttt cccagatgca tccggtgctg
     3481 cggcagatgc gcccccctcc tcagcagcgg caagagcaag agcagcggca gacatgcagg
     3541 gcaccctccc cttctcctac cgcgtcagga ggggcaacat cctacatcga ttctagtgaa
     3601 tccacagaaa ctagcgaggt aagcacttac tctatgtctt ttacatggtc ctgggaaagt
     3661 ggaaaataca ccactgaaac ttttgctacc aactcttaca ccttctccta cattgcccag
     3721 gaataaagaa tcgtgaacct gttgcatgtt atgtttcaac gtgtttattt ttcaattgca
     3781 gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata gcttatattg
     3841 atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac ctccctccca
     3901 acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca tatcatgggt
     3961 aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca aacgctcatc
     4021 agtgatatta ataaactccc cgggcagctc gcttaagttc atgtcgctgt ccagctgctg
     4081 agccacaggc tgctgtccaa cttgcggttg ctcaacgggc ggcgaagggg aagtccatgc
     4141 ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct gcagcagcgc
     4201 gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg cagtggtctc
     4261 ctcagcgatg attcgcaccg cccgcagcat gagacgcctt gtcctccggg cacagcagcg
     4321 caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa tattgttcaa
     4381 aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag aacccacgtg
     4441 gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca cgctggacat
     4501 aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata taaacctctg
     4561 attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct gcccgccggc
     4621 tatgcactgc agggaaccgg gactggaaca atgacagtgg agagcccagg actcgtaacc
     4681 atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca cgtgcataca
     4741 cttcctcagg attacaagct cctcccgcgt cagaaccata tcccagggaa caacccattc
     4801 ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca cgttgtgcat
     4861 tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag cgcgggtctc
     4921 tgtctcaaaa ggaggtaggc gatccctact gtacggagtg cgccgagaca accgagatcg
     4981 tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc ctgaagcaaa
     5041 accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgtcgctta gctcgctctg
     5101 tgtagtagtt gtagtatatc cactctctca aagcatccag gcgcctaggc aaaatagcac
     5161 cctcccgctc cagaacaaca tacagcgctt ccacagcggc agccataaca gtcagcctta
     5221 ccagtaaaaa aacctattaa aaaacaccac tcgacacggc accagctcaa tcagtcacag
     5281 tgtaaaaagg gccaagtaca gagcgagtat atataggact aaaaaatgac gtaacggtta
     5341 aagtccacaa aaaacaccca gaaaaccgca cgcgaaccta cgcccagaaa cgaaagccaa
     5401 aaaacccaca acttcctcaa atcttcactt ccgttttccc acgatacgtc acttcccatt
     5461 ttaaaaaaac tacaattccc aatacatgca agttactccg ccctaaaacg cccgggcgac
     5521 cgcaccctgt gacgaaagcc gcccgcaagc tgcgcccctg agttagtcat ctgaacttcg
     5581 gcctgggcgt ctctgggaag taccacagtg gtgggagcgg gactttcctg gtacaccagg
     5641 gcagcgggcc aactacgggg attaaggtta ttacgaggtg tggtggtaat agccgcctgt
     5701 tcgaggagaa ttcggtttcg gtgggcgcgg attccgttga cccgggatat catgtggggt
     5761 cccgcgctca tgtagtttat tcgggttgag tagtcttggg cagctccagc cgcaagtccc
     5821 atttgtggct ggtaactcca catgtagggc gtgggaattt ccttgctcat aatggcgctg
     5881 acgacaggtg ctggcgccgg gtgtggccgc tggagatgac gtagttttcg cgcttaaatt
     5941 tgagaaaggg cgcgaaacta gtccttaaga gtcagcgcgc agtatttgct gaagagagcc
     6001 tccgcgtctt ccagcgtgcg ccgaagctga tcttcgcttt tgtgatacag gcagctgcgg
     6061 gtgagggagc gcagagacct gttttttatt ttcagctctt gttcttggcc cctgctttgt
     6121 tgaaatatag catacagagt gggaaaaatc ctatttctaa gctcgcgggt cgatacgggt
     6181 tcgttgggcg ccagacgcag cgctcctcct cctgctgctg ccgccgctgt ggatttcttg
     6241 ggctttgtca gagtcttgct atccggtcgc ctttgcttct gtgtgaccgc tgctgttgct
     6301 gccgctgccg ctgccgccgg tgcagtaggg gctgtagaga tgacggtagt aatgcaggat
     6361 gttacggggg aaggccacgc cgtgatggta gagaagaaag cggcgggcga aggagatgtt
     6421 gcccccacag tcttgcaagc aagcaactat ggcgttcttg tgcccgcgcc acgagcggta
     6481 gccttggcgc tgttgttgct cttgggctaa cggcggcggc tgcttagact taccggccct
     6541 ggttccagtg gtgtcccatc tacggttggg tcggcgaaca ggcagtgccg gcggcgcctg
     6601 aggagcggag gttgtagcga tgctgggaac ggttgccaat ttctggggcg ccggcgaggg
     6661 gaatgcgacc gagggtgacg gtgtttcgtc tgacacctct tcggcctcgg aagcttcgtc
     6721 taggctgtcc cagtcttcca tcatctcctc ctcctcgtcc aaaacctcct ctgcctgact
     6781 gtcccagtat tcctcctcgt ccgtgggtgg cggcggcggc agctgcagct tctttttggg
     6841 tgccatcctg ggaagcaagg gcccgcggct gctgataggg ctgcggcggc ggggggattg
     6901 ggttgagctc ctcgccggac tgggggtcca ggtaaacccc ccgtcccttt cgtagcagaa
     6961 actcttggcg ggctttgttg atggcttgca attggccaag gatgtggccc tgggtaatga
     7021 cgcaggcggt aagctccgca tttggcgggc gggattggtc ttcgtagaac ctaatctcgt
     7081 gggcgtggta gtcctcaggg agaaggaaat ggccagtcgg gaagaggagc agcgcgaaac
     7141 cacccccgag cgcggacgcg gtgcggcgcg acgtccacca accatggagg acgtgtcgtc
     7201 cccgtcgccg tcgccgccgc ctccccgcgc gcccccaaaa aagagactgc ggagacgcct
     7261 ggagtccgag gacgaggaag acagttccca ggacgctctg gtgcctagga caccatctcc
     7321 aagaccctcc acttccaccg ccgacctggc cattgccagc aaaaaaaaga agaagaggcc
     7381 ctcccctaaa cctgaacgac cacccagccc agaagtgatc gtggactcag aggaagagcg
     7441 cgaggacgtc gctctgcaga tggtgggatt cagcaatcca cctgtgctca tcaagcacgg
     7501 caaaggggga aaacggaccg tgcgcaggct gaacgaggat gatcctgtgg caaggggcat
     7561 gaggacacag gaggagaagg aagaatcctc cgaggccgag agcgagagca cagtgattaa
     7621 tccactgtcc ctgccaattg tgagcgcttg ggagaaggga atggaggccg cccgggccct
     7681 gatggacaag taccacgtgg acaacgacct gaaggctaat ttcaaactgc tgcctgacca
     7741 ggtggaggcc ctggctgccg tgtgcaagac atggctgaat gaggagcata gagggctgca
     7801 gctgaccttt acatcaaaca agacctttgt gaccatgatg ggcaggtttc tgcaggccta
     7861 cctgcaatct ttcgccgaag tgacttacaa gcaccacgag cccaccggct gcgccctgtg
     7921 gctgcacaga tgcgccgaga ttgagggcga gctgaaatgc ctgcacggct ctatcatgat
     7981 taacaaggag cacgtgatcg agatggatgt gacttccgaa aacggccagc gcgccctgaa
     8041 ggagcagtcc agcaaggcca agatcgtgaa gaacaggtgg ggcaggaacg tggtgcagat
     8101 ttctaacaca gacgccagat gttgcgtgca tgacgccgcc tgccctgcca atcagtttag
     8161 cggcaagagc tgcggaatgt tcttcagcga gggagccaag gcgcaagtgg ccttcaagca
     8221 gatcaaggcc ttcatgcaag ccctgtatcc aaatgcccag accggccacg gccacctcct
     8281 gatgcccctg cgctgcgaat gtaactccaa gcccggccac gcccctttcc tgggcaggca
     8341 gctgccaaaa ctgacccctt ttgccctgag caacgccgaa gatctggatg ccgatctgat
     8401 cagcgacaag tccgtgctgg cctccgttca ccaccccgcc ctgatcgtgt tccagtgctg
     8461 caatcccgtg tacaggaata gcagagctca gggtggaggc cccaactgcg acttcaagat
     8521 ctccgcccca gatctgctga acgctctcgt gatggtgcgg agcctgtggt ctgagaactt
     8581 cacagagctg ccccgcatgg tggtgcccga gttcaagtgg agcactaagc atcagtaccg
     8641 gaatgtgtct ctgcccgtgg cccactctga cgccagacag aatccttttg acttctgaac
     8701 ggcgcagacg gcaagggtgg ggggtaaata atcacccgag agtgtacaaa taaaaacatt
     8761 tgcctttatt gaaagtgtct cctagtacat tatttttaca tgtttttcaa gtgacaaaaa
     8821 gaagtggcgc tcctaatctg cgcactgtgg ctgcggaagt agggcgagtg gcgctccagg
     8881 aagctgtaga gctgttcctg gttgcgacgc agggtgggct gtacctgggg actgttaagc
     8941 atggagttgg gtaccggatc catcgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     9001 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9061 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9121 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9181 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9241 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9301 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9361 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     9421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9541 ggaagagtat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     9601 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     9661 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     9721 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     9781 cagctgtact cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     9841 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     9901 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     9961 aacatcgcat cgagagagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
    10021 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
    10081 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
    10141 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
    10201 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
    10261 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
    10321 gccttcttga cgagttcttc tgactgtcag accaagttta ctcatatata ctttagattg
    10381 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10441 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10501 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10561 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10621 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10681 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10741 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10801 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10861 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10921 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10981 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    11041 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11101 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11161 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11221 ctgatcatat ggtttggcag
//
