LOCUS       sequence-458358-       15796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 
            serotype, utilizing the AAVone single-plasmid AAV production 
            system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15796)
  AUTHORS   Yang R, Tran NT, Chen T, Cui M, Wang Y, Sharma T, Liu Y, Zhang J, 
            Yuan X, Zhang D, Chen C, Shi Z, Wang L, Dai Y, Zaidi H, Liang J, 
            Chen M, Jaijyan D, Hu H, Wang B, Xu C, Hu W, Gao G, Yu D, Tai PWL, 
            Wang Q
  TITLE     AAVone: A cost-effective, single-plasmid solution for efficient AAV 
            production with reduced DNA impurities.
  JOURNAL   Mol Ther Nucleic Acids. 2025 May 14;36(2):102563. doi: 
            10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10.
  PUBMED    40503174
REFERENCE   2  (bases 1 to 15796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2025-05-14"; volume: "36(2):102563. doi"; 
            pages: " 10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10"
COMMENT     Sequence Label: pAAVone-AAV9-CMV-eGFP
FEATURES             Location/Qualifiers
     source          1..15796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        134..512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     667..687
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          717..813
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      818..827
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(824..826,827..829,830..1543)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(824..826,827..829,830..1540)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(869..890)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1130..1149)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1477..1498
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1568..2156
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1621..1641)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2039..2050)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2188..2664
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2387..2406)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2673..2802
                     /label=AAV2 ITR
     CDS             complement(4050..4061)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     rep_origin      13513..13967
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13599..13618)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13809..13830
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13993..14097
                     /gene="bla"
                     /label=AmpR promoter
     CDS             14098..14892
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             14098..14892
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14152..14171)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14762..14781
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      15063..15651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15552..15571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcgtcgg gcgacctttg gtcgcccggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
      121 cggccgcacg cgtacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      181 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc acctgcgtaa
      721 gtttagtctt tttgtctttt atttcaggtc ccggatccgg tggtggtgca aatcaaagaa
      781 ctgctcctca gtggatgttg cctttacttc taggcgcgcc accatggtga gcaagggcga
      841 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      901 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      961 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1021 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1081 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1141 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1201 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1261 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1321 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1381 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1441 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1501 cgccgccggg atcactctcg gcatggacga gctgtacaag taacgtacgg atatcaagct
     1561 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1621 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1681 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1741 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1801 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1861 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1921 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     1981 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2041 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2101 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2161 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     2221 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2281 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     2341 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     2401 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     2461 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     2521 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     2581 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     2641 accactgctc ccttccctgt ccttttaatt aaaggaaccc ctagtgatgg agttggccac
     2701 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     2761 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgccaac cggtgtcgac
     2821 gtaatccgta gatgtacctg gacatccagg tgatgccggc ggcggtggtg gaggcgcgcg
     2881 gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa aaagtgctcc atggtcggga
     2941 cgctctggcc ggtgaggcgt gcgcagtcgt tgacgctcta gaccgtgcaa aaggagagcc
     3001 tgtaagcggg cactcttccg tggtctggtg gataaattcg caagggtatc atggcggacg
     3061 accggggttc gaaccccgga tccggccgtc cgccgtgatc catgcggtta ccgcccgcgt
     3121 gtcgaaccca ggtgtgcgac gtcagacaac gggggagcgc tccttttggc ttccttccag
     3181 gcgcggcggc tgctgcgcta gcttttttgg ccactggccg cgcgcggcgt aagcggttag
     3241 gctggaaagc gaaagcatta agtggctcgc tccctgtagc cggagggtta ttttccaagg
     3301 gttgagtcgc aggacccccg gttcgagtct cgggccggcc ggactgcggc gaacgggggt
     3361 ttgcctcccc gtcatgcaag accccgcttg caaattcctc cggaaacagg gacgagcccc
     3421 ttttttgctt ttcccagatg catccggtgc tgcggcagat gcgcccccct cctcagcagc
     3481 ggcaagagca agagcagcgg cagacatgca gggcaccctc cccttctcct accgcgtcag
     3541 gaggggcaac atcctacatc gatagatctg cagaattcgg cttggcggct gcgcgttcaa
     3601 acctcccgct tcaaaatgga gaccctgcgt gctcactcgg gcttaaatac ccagcgtgac
     3661 cacatggtgt cgcaaaatgt cgcaaaacac tcacgtgacc tctaatacag gacctctaga
     3721 gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaaag ccgaattcca
     3781 gcacactggc ggccgttact agtggatcct agagcatgga aactagataa gaaagaaata
     3841 cgcagagacc aaagttcaac tgaaacgaat taaacggttt attgattaac aagcgatcgt
     3901 tattacagat tacgagtcag gtatctggtg ccaatggggc ggggttcact atatacacct
     3961 tcagtattaa cagcaaattc aacattatta gacttgtaat agttggaagt gtactggatc
     4021 tccgggttcc agcgcttgct gttttccttc tgcagctccc actcgatctc cacgctgact
     4081 tggccagtag aatactgggt gatgaaagag ttcagcttgt ccttgttgaa ggccgttgga
     4141 ggatccgcag gtacaggtgt gtttttgatg aggatctgag gaggcgggtg cttcattcca
     4201 aaccctccca tcagcggaga agggtgaaag ttgccgtccg tgtgaggaat tttggcccaa
     4261 atgggtcctt gcaggtacac atctctgtcc tgccaaacca tacccggaag tattccttgg
     4321 ttttgaaccc agccggtctg cgcctgtgct tgggcactct ggtggtttgt ggccacttgt
     4381 ccataggact ccgttgctac cgggttagta gttttaattt cttcttcgtt ggttatcatg
     4441 actttgtccg catccacgtt gtctcttcca gttccttgtt tgccaaaaat taaagatcca
     4501 gacaaaggaa agaaacggtc ctctccttct ttgtggctgg ccatagcagg tccaggattc
     4561 atcaagctat tacgtccatt gagagcccaa gaagaagctc caggccaagc aaattcgctg
     4621 ttgttgtttt gagtcacagt ggttgagaca cgttgttgtc ggtagctggg tccaggtatg
     4681 tagtttcttc cctggacagc catgttgctg ggtccggcca cactgaattt tagcgtttgt
     4741 tgattctgtc cagaaccgtt aatagtcttt gagagatagt acaagtattg gtcgatgagt
     4801 ggattcatta gtcggtccag gctttggctg tgagcgtagc tgctatggaa aggtacgttc
     4861 tcaaactcgt agctgaactg gaagttgtta cccgttctta gcatttgcga cgggaaatat
     4921 tccaggcagt aaaaggacga acgacccacg gcctggcttc catcattaag cgtcagatac
     4981 ccgtactgag gaatcatgaa aacgtccgct gggaacggcg ggaggcagcc ctcgtgagcc
     5041 gacccgagca cgtacgggag ctgatagtct gagtccgtga agacctggac cgtgctggta
     5101 aggttattgg cgatggtctt gactccattg ttgtccgtaa cctctttgac ctgaatgttg
     5161 aagagcttga agttgagtcg cttaggccgg aatccccagt tgttgttgat gagtcgctgc
     5221 cagtcacgtg gtgagaagtg gcagtggaat ctgttgaagt caaaataccc ccagggggtg
     5281 ctgtagccga agtaggcgtt gtcatttgaa gatcctccag atgtgctgtt ggagatttgc
     5341 ttgtagaggt gattgttgta ggtgggcagg gcccaggttc gggtgctggt ggtgatgact
     5401 ctgtccccca gccattggga atcgcaatgc caatttcccg aggaactacc cactccatcg
     5461 gcaccttcgt tattgtctgc cactggtgcg ccaccacctg aagccattgt aagagatccc
     5521 acacctgagg gggctgcggg aggttctccg attggttgag ggtctgggac tgactctgtg
     5581 tcgccagtct gaccgaaatt gagtctcttt ttagcgggct gtgcacccga tttgccaata
     5641 cccgcggagg agtccggttc ctgaggagac tgctctacag gcctcttctt tccaggagcc
     5701 gtcttagccg cttcctcaac cagaccaaga ggttcaagaa gcctcttttt ggcctggaag
     5761 actgctcgcc cgaggttgcc cccaaaagac gtatcttctt tgagccgctc ctggaactcg
     5821 gcgtcggcgt ggttgtactt gaggtacggg ttgtctccgg ccttgagctg ctggtcgtag
     5881 gccttgtcgt gctcgagggc cgccgcgtct gctgcgttga ccggctcccc cttgtcgagt
     5941 ccgttgccgg gtccaaggta tttgtaaccc ggaagcacaa gacctcgagc gttgtcttga
     6001 tgttgttgat ttgccttggg ttgaggggct ccaggtttca aagcccacca ctcgcgaatt
     6061 ccttcactaa ggttgtcctc gagccaatct ggaagataac catcggcagc catacctgat
     6121 ttaaatcatt tattgttcaa agatgcagtc atccaaatcc acattgacca gatcgcaggc
     6181 agtgcaagcg tctggcacct ttcccatgat atgatgaatg tagcacagtt tctgatacgc
     6241 ctttttgacg acagaaacgg gttgagattc tgacacggga aagcactcta aacagtcttt
     6301 ctgtccgtga gtgaagcaga tatttgaatt ctgattcatt ctctcgcatt gtctgcaggg
     6361 aaacagcatc agattcatgc ccacgtgacg agaacatttg tttacgtacc tgtctgcgta
     6421 gttgatcgaa gcttccgcgt ctgacgtcga tggctgcgca actgactcgc gcacccgttt
     6481 gggctcactt atatctgcgt cactgggggc gggtcttttc ttggctccac cctttttgac
     6541 gtagaattca tgctccacct caaccacgtg atcctttgcc caccggaaaa agtctttgac
     6601 ttcctgcttg gtgaccttcc caaagtcatg atccagacgg cgggtgagtt caaatttgaa
     6661 catccggtct tgcaacggct gctggtgttc gaaggtcgtt gagttcccgt caatcacggc
     6721 gcacatgttg gtgttggagg tgacgatcac gggagtcggg tctatctggg ccgaggactt
     6781 gcatttctgg tccacgcgca ccttgcttcc tccgagaatg gctttggccg actccacgac
     6841 cttggcggtc atcttcccct cctcccacca gatcaccatc ttgtcgacac agtcgttgaa
     6901 gggaaagttc tcattggtcc agtttacgca cccgtagaag ggcacagtgt gggctatggc
     6961 ctccgcgatg ttggtcttcc cggtagttgc aggcccaaac agccagatgg tgttcctctt
     7021 gccgaacttt ttcgtggccc atcccagaaa gacggaagcc gcatattggg gatcgtaccc
     7081 gtttagttcc aaaattttat aaatccgatt gctggaaatg tcctccacgg gctgctggcc
     7141 caccaggtag tcgggggcgg ttttagtcag gctcataatc tttcccgcat tgtccaaggc
     7201 agccttgatt tgggaccgcg agttggaggc cgcattgaag gagatgtatg aggcctggtc
     7261 ctcctggatc cactgcttct ccgaggtaat ccccttgtcc acgagccacc cgaccagctc
     7321 catgtacctg gctgaagttt ttgatctgat caccggcgca tcagaattgg gattctgatt
     7381 ctctttgttc tgctcctgcg tctgcgacac gtgcgtcaga tgctgcgcca ccaaccgttt
     7441 acgctccgtg agattcaaac aggcgcttaa atactgttcc atattagtcc acgcccactg
     7501 gagctcaggc tgggttttgg ggagcaagta attggggatg tagcactcat ccaccacctt
     7561 gttcccgcct ccggcgccat ttctggtctt tgtgaccgcg aaccagtttg gcaaagtcgg
     7621 ctcgatcccg cggtaaattc tctgaatcag tttttcgcga atctgactca ggaaacgtcc
     7681 caaaaccatg gatttcaccc cggtggtttc cacgagcacg tgcatgtgga agtagctctc
     7741 tcccttctca aattgcacaa agaaaagagc ctccggtgcc ttactcacac ggcgccattc
     7801 cgtcagaaag tcgcgctgca gcttctcggc cacggtcagg ggtgcctgct caatcagatt
     7861 cagatccatg tcagaatctg gcggcaactc ccattccttc tcggccaccc agttcacaaa
     7921 gctgtcagaa atgccgggca gatgctcgtc aaggtcgctg gggaccttaa tcacaatctc
     7981 gtaaaacccc ggcatggcgg ctgctcgttc aaatttcgcg gtcaaaatgg aggttgcatc
     8041 ctgctcgctc actcggccat atatacctca gcgtgaccac atggtgtcgc aaaatgtcgc
     8101 aaaacactca cgttgacctc taatacagga ccgcggccgc tctagtgaat ccacagaaac
     8161 tagcgaggta agcacttact ctatgtcttt tacatggtcc tgggaaagtg gaaaatacac
     8221 cactgaaact tttgctacca actcttacac cttctcctac attgcccagg aataaagaat
     8281 cgtgaacctg ttgcatgtta tgtttcaacg tgtttatttt tcaattgcag aaaatttcaa
     8341 gtcatttttc attcagtagt atagccccac caccacatag cttatattga tcaccgtacc
     8401 ttaatcaaac tcacagaacc ctagtattca acctgccacc tccctcccaa cacacagagt
     8461 acacagtcct ttctccccgg ctggccttaa aaagcatcat atcatgggta acagacatat
     8521 tcttaggtgt tatattccac acggtttcct gtcgagccaa acgctcatca gtgatattaa
     8581 taaactcccc gggcagctcg cttaagttca tgtcgctgtc cagctgctga gccacaggct
     8641 gctgtccaac ttgcggttgc tcaacgggcg gcgaagggga agtccatgcc tacatggggg
     8701 tagagccata atcgtgcatc aggatagggc ggtggtgctg cagcagcgcg cgaataaact
     8761 gctgccgccg ccgctccgtc ctgcaggaat acaacatggc agtggtctcc tcagcgatga
     8821 ttcgcaccgc ccgcagcatg agacgccttg tcctccgggc acagcagcgc accctgatct
     8881 cacttaaatc agcacagtaa ctgcagcaca gcaccacaat attgttcaaa atcccacagt
     8941 gcaaggcgct gtatccaaag ctcatggcgg ggaccacaga acccacgtgg ccatcatacc
     9001 acaagcgcag gtagattaag tggcgacccc tcataaacac gctggacata aacattacct
     9061 cttttggcat gttgtaattc accacctccc ggtaccatat aaacctctga ttaaacatgg
     9121 cgccatccac caccatccta aaccagctgg ccaaaacctg cccgccggct atgcactgca
     9181 gggaaccggg actggaacaa tgacagtgga gagcccagga ctcgtaacca tggatcatca
     9241 tgctcgtcat gatatcaatg ttggcacaac acaggcacac gtgcatacac ttcctcagga
     9301 ttacaagctc ctcccgcgtc agaaccatat cccagggaac aacccattcc tgaatcagcg
     9361 taaatcccac actgcaggga agacctcgca cgtaactcac gttgtgcatt gtcaaagtgt
     9421 tacattcggg cagcagcgga tgatcctcca gtatggtagc gcgggtctct gtctcaaaag
     9481 gaggtaggcg atccctactg tacggagtgc gccgagacaa ccgagatcgt gttggtcgta
     9541 gtgtcatgcc aaatggaacg ccggacgtag tcatatttcc tgaagcaaaa ccaggtgcgg
     9601 gcgtgacaaa cagatctgcg tctccggtct cgtcgcttag ctcgctctgt gtagtagttg
     9661 tagtatatcc actctctcaa agcatccagg cgcctaggca aaatagcacc ctcccgctcc
     9721 agaacaacat acagcgcttc cacagcggca gccataacag tcagccttac cagtaaaaaa
     9781 acctattaaa aaacaccact cgacacggca ccagctcaat cagtcacagt gtaaaaaggg
     9841 ccaagtacag agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa
     9901 aaacacccag aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa
     9961 cttcctcaaa tcttcacttc cgttttccca cgatacgtca cttcccattt taaaaaaact
    10021 acaattccca atacatgcaa gttactccgc cctaaaacgc ccgggcgacc gcaccctgtg
    10081 acgaaagccg cccgcaagct gcgcccctga gttagtcatc tgaacttcgg cctgggcgtc
    10141 tctgggaagt accacagtgg tgggagcggg actttcctgg tacaccaggg cagcgggcca
    10201 actacgggga ttaaggttat tacgaggtgt ggtggtaata gccgcctgtt cgaggagaat
    10261 tcggtttcgg tgggcgcgga ttccgttgac ccgggatatc atgtggggtc ccgcgctcat
    10321 gtagtttatt cgggttgagt agtcttgggc agctccagcc gcaagtccca tttgtggctg
    10381 gtaactccac atgtagggcg tgggaatttc cttgctcata atggcgctga cgacaggtgc
    10441 tggcgccggg tgtggccgct ggagatgacg tagttttcgc gcttaaattt gagaaagggc
    10501 gcgaaactag tccttaagag tcagcgcgca gtatttgctg aagagagcct ccgcgtcttc
    10561 cagcgtgcgc cgaagctgat cttcgctttt gtgatacagg cagctgcggg tgagggagcg
    10621 cagagacctg ttttttattt tcagctcttg ttcttggccc ctgctttgtt gaaatatagc
    10681 atacagagtg ggaaaaatcc tatttctaag ctcgcgggtc gatacgggtt cgttgggcgc
    10741 cagacgcagc gctcctcctc ctgctgctgc cgccgctgtg gatttcttgg gctttgtcag
    10801 agtcttgcta tccggtcgcc tttgcttctg tgtgaccgct gctgttgctg ccgctgccgc
    10861 tgccgccggt gcagtagggg ctgtagagat gacggtagta atgcaggatg ttacggggga
    10921 aggccacgcc gtgatggtag agaagaaagc ggcgggcgaa ggagatgttg cccccacagt
    10981 cttgcaagca agcaactatg gcgttcttgt gcccgcgcca cgagcggtag ccttggcgct
    11041 gttgttgctc ttgggctaac ggcggcggct gcttagactt accggccctg gttccagtgg
    11101 tgtcccatct acggttgggt cggcgaacag gcagtgccgg cggcgcctga ggagcggagg
    11161 ttgtagcgat gctgggaacg gttgccaatt tctggggcgc cggcgagggg aatgcgaccg
    11221 agggtgacgg tgtttcgtct gacacctctt cggcctcgga agcttcgtct aggctgtccc
    11281 agtcttccat catctcctcc tcctcgtcca aaacctcctc tgcctgactg tcccagtatt
    11341 cctcctcgtc cgtgggtggc ggcggcggca gctgcagctt ctttttgggt gccatcctgg
    11401 gaagcaaggg cccgcggctg ctgatagggc tgcggcggcg gggggattgg gttgagctcc
    11461 tcgccggact gggggtccag gtaaaccccc cgtccctttc gtagcagaaa ctcttggcgg
    11521 gctttgttga tggcttgcaa ttggccaagg atgtggccct gggtaatgac gcaggcggta
    11581 agctccgcat ttggcgggcg ggattggtct tcgtagaacc taatctcgtg ggcgtggtag
    11641 tcctcaggga gaaggaaatg gccagtcggg aagaggagca gcgcgaaacc acccccgagc
    11701 gcggacgcgg tgcggcgcga cgtccaccaa ccatggagga cgtgtcgtcc ccgtcgccgt
    11761 cgccgccgcc tccccgcgcg cccccaaaaa agagactgcg gagacgcctg gagtccgagg
    11821 acgaggaaga cagttcccag gacgctctgg tgcctaggac accatctcca agaccctcca
    11881 cttccaccgc cgacctggcc attgccagca aaaaaaagaa gaagaggccc tcccctaaac
    11941 ctgaacgacc acccagccca gaagtgatcg tggactcaga ggaagagcgc gaggacgtcg
    12001 ctctgcagat ggtgggattc agcaatccac ctgtgctcat caagcacggc aaagggggaa
    12061 aacggaccgt gcgcaggctg aacgaggatg atcctgtggc aaggggcatg aggacacagg
    12121 aggagaagga agaatcctcc gaggccgaga gcgagagcac agtgattaat ccactgtccc
    12181 tgccaattgt gagcgcttgg gagaagggaa tggaggccgc ccgggccctg atggacaagt
    12241 accacgtgga caacgacctg aaggctaatt tcaaactgct gcctgaccag gtggaggccc
    12301 tggctgccgt gtgcaagaca tggctgaatg aggagcatag agggctgcag ctgaccttta
    12361 catcaaacaa gacctttgtg accatgatgg gcaggtttct gcaggcctac ctgcaatctt
    12421 tcgccgaagt gacttacaag caccacgagc ccaccggctg cgccctgtgg ctgcacagat
    12481 gcgccgagat tgagggcgag ctgaaatgcc tgcacggctc tatcatgatt aacaaggagc
    12541 acgtgatcga gatggatgtg acttccgaaa acggccagcg cgccctgaag gagcagtcca
    12601 gcaaggccaa gatcgtgaag aacaggtggg gcaggaacgt ggtgcagatt tctaacacag
    12661 acgccagatg ttgcgtgcat gacgccgcct gccctgccaa tcagtttagc ggcaagagct
    12721 gcggaatgtt cttcagcgag ggagccaagg cgcaagtggc cttcaagcag atcaaggcct
    12781 tcatgcaagc cctgtatcca aatgcccaga ccggccacgg ccacctcctg atgcccctgc
    12841 gctgcgaatg taactccaag cccggccacg cccctttcct gggcaggcag ctgccaaaac
    12901 tgaccccttt tgccctgagc aacgccgaag atctggatgc cgatctgatc agcgacaagt
    12961 ccgtgctggc ctccgttcac caccccgccc tgatcgtgtt ccagtgctgc aatcccgtgt
    13021 acaggaatag cagagctcag ggtggaggcc ccaactgcga cttcaagatc tccgccccag
    13081 atctgctgaa cgctctcgtg atggtgcgga gcctgtggtc tgagaacttc acagagctgc
    13141 cccgcatggt ggtgcccgag ttcaagtgga gcactaagca tcagtaccgg aatgtgtctc
    13201 tgcccgtggc ccactctgac gccagacaga atccttttga cttctgaacg gcgcagacgg
    13261 caagggtggg gggtaaataa tcacccgaga gtgtacaaat aaaaacattt gcctttattg
    13321 aaagtgtctc ctagtacatt atttttacat gtttttcaag tgacaaaaag aagtggcgct
    13381 cctaatctgc gcactgtggc tgcggaagta gggcgagtgg cgctccagga agctgtagag
    13441 ctgttcctgg ttgcgacgca gggtgggctg tacctgggga ctgttaagca tggagttggg
    13501 taccggatcc atcgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
    13561 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
    13621 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
    13681 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
    13741 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
    13801 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
    13861 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
    13921 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
    13981 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
    14041 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
    14101 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
    14161 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
    14221 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
    14281 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtactc
    14341 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
    14401 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
    14461 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
    14521 gagagagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
    14581 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
    14641 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
    14701 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
    14761 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
    14821 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
    14881 gagttcttct gactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    14941 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    15001 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    15061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    15121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    15181 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    15241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    15301 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    15361 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    15421 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    15481 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    15541 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    15601 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    15661 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    15721 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgatcatatg
    15781 gtttaaacgt tggcag
//
