LOCUS       sequence-458359-       15790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 
            serotype, utilizing the AAVone single-plasmid AAV production 
            system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15790)
  AUTHORS   Yang R, Tran NT, Chen T, Cui M, Wang Y, Sharma T, Liu Y, Zhang J, 
            Yuan X, Zhang D, Chen C, Shi Z, Wang L, Dai Y, Zaidi H, Liang J, 
            Chen M, Jaijyan D, Hu H, Wang B, Xu C, Hu W, Gao G, Yu D, Tai PWL, 
            Wang Q
  TITLE     AAVone: A cost-effective, single-plasmid solution for efficient AAV 
            production with reduced DNA impurities.
  JOURNAL   Mol Ther Nucleic Acids. 2025 May 14;36(2):102563. doi: 
            10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10.
  PUBMED    40503174
REFERENCE   2  (bases 1 to 15790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2025-05-14"; volume: "36(2):102563. doi"; 
            pages: " 10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10"
COMMENT     Sequence Label: pAAVone-AAV2-CMV-eGFP
FEATURES             Location/Qualifiers
     source          1..15790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        134..512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     667..687
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          717..813
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      818..827
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(824..826,827..829,830..1543)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(824..826,827..829,830..1540)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(869..890)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1130..1149)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1477..1498
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1568..2156
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1621..1641)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2039..2050)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2188..2664
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2387..2406)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2673..2802
                     /label=AAV2 ITR
     CDS             complement(4047..4058)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     rep_origin      13507..13961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13593..13612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13803..13824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13987..14091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             14092..14886
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             14092..14886
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14146..14165)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14756..14775
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      15057..15645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15546..15565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcgtcgg gcgacctttg gtcgcccggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
      121 cggccgcacg cgtacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      181 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc acctgcgtaa
      721 gtttagtctt tttgtctttt atttcaggtc ccggatccgg tggtggtgca aatcaaagaa
      781 ctgctcctca gtggatgttg cctttacttc taggcgcgcc accatggtga gcaagggcga
      841 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      901 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      961 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1021 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1081 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1141 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1201 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1261 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1321 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1381 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1441 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1501 cgccgccggg atcactctcg gcatggacga gctgtacaag taacgtacgg atatcaagct
     1561 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1621 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1681 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1741 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1801 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1861 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1921 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     1981 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2041 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2101 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2161 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     2221 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2281 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     2341 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     2401 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     2461 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     2521 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     2581 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     2641 accactgctc ccttccctgt ccttttaatt aaaggaaccc ctagtgatgg agttggccac
     2701 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     2761 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgccaac cggtgtcgac
     2821 gtaatccgta gatgtacctg gacatccagg tgatgccggc ggcggtggtg gaggcgcgcg
     2881 gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa aaagtgctcc atggtcggga
     2941 cgctctggcc ggtgaggcgt gcgcagtcgt tgacgctcta gaccgtgcaa aaggagagcc
     3001 tgtaagcggg cactcttccg tggtctggtg gataaattcg caagggtatc atggcggacg
     3061 accggggttc gaaccccgga tccggccgtc cgccgtgatc catgcggtta ccgcccgcgt
     3121 gtcgaaccca ggtgtgcgac gtcagacaac gggggagcgc tccttttggc ttccttccag
     3181 gcgcggcggc tgctgcgcta gcttttttgg ccactggccg cgcgcggcgt aagcggttag
     3241 gctggaaagc gaaagcatta agtggctcgc tccctgtagc cggagggtta ttttccaagg
     3301 gttgagtcgc aggacccccg gttcgagtct cgggccggcc ggactgcggc gaacgggggt
     3361 ttgcctcccc gtcatgcaag accccgcttg caaattcctc cggaaacagg gacgagcccc
     3421 ttttttgctt ttcccagatg catccggtgc tgcggcagat gcgcccccct cctcagcagc
     3481 ggcaagagca agagcagcgg cagacatgca gggcaccctc cccttctcct accgcgtcag
     3541 gaggggcaac atcctacatc gatagatctg cagaattcgg cttggcggct gcgcgttcaa
     3601 acctcccgct tcaaaatgga gaccctgcgt gctcactcgg gcttaaatac ccagcgtgac
     3661 cacatggtgt cgcaaaatgt cgcaaaacac tcacgtgacc tctaatacag gacctctaga
     3721 gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaaag ccgaattcca
     3781 gcacactggc ggccgttact agtggatcct agagcatgga aactagataa gaaagaaata
     3841 cgcagagacc aaagttcaac tgaaacgaat taaacggttt attgattaac aagcaattgt
     3901 tacagattac gagtcaggta tctggtgcca atggggcgag gctctgaata cacgccatta
     3961 gtgtccacag taaagtccac attaacagac ttgttgtagt tggaagtgta ctgaatttcg
     4021 ggattccagc gtttgctgtt ttccttctgc agctcccact cgatctccac gctgacctgt
     4081 cccgtggagt actgtgtgat gaaggaagca aactttgccg cactgaaggt ggtcgaagga
     4141 ttcgctggta ccggggtgtt cttgatgaga atctgtggag gagggtgttt aagtccgaat
     4201 ccacccatga ggggagaggg gtgaaaatgt ccgtccgtgt gtggaatctt tgcccagatg
     4261 ggcccctgaa ggtacacatc tctgtcctgc cagaccatgc ctggaagaac gccttgtgtg
     4321 ttgacatctg cggtagctgc ttgtctgttg cctctctgga ggttggtaga tacagaacca
     4381 tactgctccg tagccacggg attggttgtc ctgatttcct cttcgtctgt aatcatgacc
     4441 ttttcaatgt ccacatttgt tttctctgag ccttgcttcc caaagatgag aaccccgctc
     4501 tgaggaaaaa acttttcttc atcgtccttg tggcttgcca tggccgggcc cggattcacc
     4561 agagagtctc tgccattgag gtggtacttg gtagctccag tccacgagta ttcactgttg
     4621 ttgttatccg cagatgtctt tgatactcgc tgctggcggt aacagggtcc aggaagccag
     4681 ttcctagact ggtcccgaat gtcactcgct ccggcctgag aaaactgaag ccttgactgc
     4741 gtggtggttc cacttggagt gtttgttctg ctcaagtaat acaggtactg gtcgatgaga
     4801 ggattcatga gacggtccag actctggctg tgagcgtagc tgctgtggaa aggaacgtcc
     4861 tcaaaagtgt agctgaaggt aaagttgttt ccggtacgca gcatctgaga aggaaagtac
     4921 tccaggcagt aaaatgaaga gcgtcctact gcctgactcc cgttgttcag ggtgaggtat
     4981 ccatactgtg gcaccatgaa gacgtctgct gggaacggcg ggaggcatcc ttgatgcgcc
     5041 gagccgagga cgtacgggag ctggtactcc gagtcagtaa acacctgaac cgtgctggta
     5101 aggttattgg caatcgtcgt cgtaccgtca ttctgcgtga cctctttgac ttgaatgtta
     5161 aagagcttga agttgagtct cttgggtcgg aatccccagt tgttgttgat gagtctttgc
     5221 cagtcacgtg gtgaaaagtg gcagtggaat ctgttgaagt caaaataccc ccaaggggtg
     5281 ctgtagccaa agtagtgatt gtcgttcgag gctcctgatt ggctggaaat ttgtttgtag
     5341 aggtggttgt tgtaggtggg cagggcccag gttcgggtgc tggtggtgat gactctgtcg
     5401 cccatccatg tggaatcgca atgccaattt cccgaggaat tacccactcc gtcggcgccc
     5461 tcgttattgt ctgccattgg tgcgccactg cctgtagcca tcgtattagt tcccagacca
     5521 gagggggctg ctggtggctg tccgagaggc tgggggtcag gtactgagtc tgcgtctcca
     5581 gtctgaccaa aattcaatct ttttcttgca ggctgctggc ccgcctttcc ggttcccgag
     5641 gaggagtctg gctccacagg agagtgctct accggcctct tttttcccgg agccgtctta
     5701 acaggttcct caaccaggcc cagaggttca agaaccctct ttttcgcctg gaagactgct
     5761 cgtccgaggt tgcccccaaa agacgtatct tctttaaggc gctcctgaaa ctccgcgtcg
     5821 gcgtggttgt acttgaggta cgggttgtct ccgctgtcga gctgccggtc gtaggctttg
     5881 tcgtgctcga gggccgcggc gtctgcctcg ttgaccggct ctcccttgtc gagtccgttg
     5941 aagggtccga ggtacttgta cccaggaagc acaagacccc tgctgtcgtc cttatgccgc
     6001 tctgcgggct ttggtggtgg tgggccaggt ttgagcttcc accactgtct tattccttca
     6061 gagagagtgt cctcgagcca atctggaaga taaccatcgg cagccatacc tgatttaaat
     6121 catttattgt tcaaagatgc agtcatccaa atccacattg accagatcgc aggcagtgca
     6181 agcgtctggc acctttccca tgatatgatg aatgtagcac agtttctgat acgccttttt
     6241 gacgacagaa acgggttgag attctgacac gggaaagcac tctaaacagt ctttctgtcc
     6301 gtgagtgaag cagatatttg aattctgatt cattctctcg cattgtctgc agggaaacag
     6361 catcagattc atgcccacgt gacgagaaca tttgtttacg tacctgtctg cgtagttgat
     6421 cgaagcttcc gcgtctgacg tcgatggctg cgcaactgac tcgcgcaccc gtttgggctc
     6481 acttatatct gcgtcactgg gggcgggtct tttcttggct ccaccctttt tgacgtagaa
     6541 ttcatgctcc acctcaacca cgtgatcctt tgcccaccgg aaaaagtctt tgacttcctg
     6601 cttggtgacc ttcccaaagt catgatccag acggcgggtg agttcaaatt tgaacatccg
     6661 gtcttgcaac ggctgctggt gttcgaaggt cgttgagttc ccgtcaatca cggcgcacat
     6721 gttggtgttg gaggtgacga tcacgggagt cgggtctatc tgggccgagg acttgcattt
     6781 ctggtccacg cgcaccttgc ttcctccgag aatggctttg gccgactcca cgaccttggc
     6841 ggtcatcttc ccctcctccc accagatcac catcttgtcg acacagtcgt tgaagggaaa
     6901 gttctcattg gtccagttta cgcacccgta gaagggcaca gtgtgggcta tggcctccgc
     6961 gatgttggtc ttcccggtag ttgcaggccc aaacagccag atggtgttcc tcttgccgaa
     7021 ctttttcgtg gcccatccca gaaagacgga agccgcatat tggggatcgt acccgtttag
     7081 ttccaaaatt ttataaatcc gattgctgga aatgtcctcc acgggctgct ggcccaccag
     7141 gtagtcgggg gcggttttag tcaggctcat aatctttccc gcattgtcca aggcagcctt
     7201 gatttgggac cgcgagttgg aggccgcatt gaaggagatg tatgaggcct ggtcctcctg
     7261 gatccactgc ttctccgagg taatcccctt gtccacgagc cacccgacca gctccatgta
     7321 cctggctgaa gtttttgatc tgatcaccgg cgcatcagaa ttgggattct gattctcttt
     7381 gttctgctcc tgcgtctgcg acacgtgcgt cagatgctgc gccaccaacc gtttacgctc
     7441 cgtgagattc aaacaggcgc ttaaatactg ttccatatta gtccacgccc actggagctc
     7501 aggctgggtt ttggggagca agtaattggg gatgtagcac tcatccacca ccttgttccc
     7561 gcctccggcg ccatttctgg tctttgtgac cgcgaaccag tttggcaaag tcggctcgat
     7621 cccgcggtaa attctctgaa tcagtttttc gcgaatctga ctcaggaaac gtcccaaaac
     7681 catggatttc accccggtgg tttccacgag cacgtgcatg tggaagtagc tctctccctt
     7741 ctcaaattgc acaaagaaaa gagcctccgg tgccttactc acacggcgcc attccgtcag
     7801 aaagtcgcgc tgcagcttct cggccacggt caggggtgcc tgctcaatca gattcagatc
     7861 catgtcagaa tctggcggca actcccattc cttctcggcc acccagttca caaagctgtc
     7921 agaaatgccg ggcagatgct cgtcaaggtc gctggggacc ttaatcacaa tctcgtaaaa
     7981 ccccggcatg gcggctgctc gttcaaattt cgcggtcaaa atggaggttg catcctgctc
     8041 gctcactcgg ccatatatac ctcagcgtga ccacatggtg tcgcaaaatg tcgcaaaaca
     8101 ctcacgttga cctctaatac aggaccgcgg ccgctctagt gaatccacag aaactagcga
     8161 ggtaagcact tactctatgt cttttacatg gtcctgggaa agtggaaaat acaccactga
     8221 aacttttgct accaactctt acaccttctc ctacattgcc caggaataaa gaatcgtgaa
     8281 cctgttgcat gttatgtttc aacgtgttta tttttcaatt gcagaaaatt tcaagtcatt
     8341 tttcattcag tagtatagcc ccaccaccac atagcttata ttgatcaccg taccttaatc
     8401 aaactcacag aaccctagta ttcaacctgc cacctccctc ccaacacaca gagtacacag
     8461 tcctttctcc ccggctggcc ttaaaaagca tcatatcatg ggtaacagac atattcttag
     8521 gtgttatatt ccacacggtt tcctgtcgag ccaaacgctc atcagtgata ttaataaact
     8581 ccccgggcag ctcgcttaag ttcatgtcgc tgtccagctg ctgagccaca ggctgctgtc
     8641 caacttgcgg ttgctcaacg ggcggcgaag gggaagtcca tgcctacatg ggggtagagc
     8701 cataatcgtg catcaggata gggcggtggt gctgcagcag cgcgcgaata aactgctgcc
     8761 gccgccgctc cgtcctgcag gaatacaaca tggcagtggt ctcctcagcg atgattcgca
     8821 ccgcccgcag catgagacgc cttgtcctcc gggcacagca gcgcaccctg atctcactta
     8881 aatcagcaca gtaactgcag cacagcacca caatattgtt caaaatccca cagtgcaagg
     8941 cgctgtatcc aaagctcatg gcggggacca cagaacccac gtggccatca taccacaagc
     9001 gcaggtagat taagtggcga cccctcataa acacgctgga cataaacatt acctcttttg
     9061 gcatgttgta attcaccacc tcccggtacc atataaacct ctgattaaac atggcgccat
     9121 ccaccaccat cctaaaccag ctggccaaaa cctgcccgcc ggctatgcac tgcagggaac
     9181 cgggactgga acaatgacag tggagagccc aggactcgta accatggatc atcatgctcg
     9241 tcatgatatc aatgttggca caacacaggc acacgtgcat acacttcctc aggattacaa
     9301 gctcctcccg cgtcagaacc atatcccagg gaacaaccca ttcctgaatc agcgtaaatc
     9361 ccacactgca gggaagacct cgcacgtaac tcacgttgtg cattgtcaaa gtgttacatt
     9421 cgggcagcag cggatgatcc tccagtatgg tagcgcgggt ctctgtctca aaaggaggta
     9481 ggcgatccct actgtacgga gtgcgccgag acaaccgaga tcgtgttggt cgtagtgtca
     9541 tgccaaatgg aacgccggac gtagtcatat ttcctgaagc aaaaccaggt gcgggcgtga
     9601 caaacagatc tgcgtctccg gtctcgtcgc ttagctcgct ctgtgtagta gttgtagtat
     9661 atccactctc tcaaagcatc caggcgccta ggcaaaatag caccctcccg ctccagaaca
     9721 acatacagcg cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaaacctat
     9781 taaaaaacac cactcgacac ggcaccagct caatcagtca cagtgtaaaa agggccaagt
     9841 acagagcgag tatatatagg actaaaaaat gacgtaacgg ttaaagtcca caaaaaacac
     9901 ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc acaacttcct
     9961 caaatcttca cttccgtttt cccacgatac gtcacttccc attttaaaaa aactacaatt
    10021 cccaatacat gcaagttact ccgccctaaa acgcccgggc gaccgcaccc tgtgacgaaa
    10081 gccgcccgca agctgcgccc ctgagttagt catctgaact tcggcctggg cgtctctggg
    10141 aagtaccaca gtggtgggag cgggactttc ctggtacacc agggcagcgg gccaactacg
    10201 gggattaagg ttattacgag gtgtggtggt aatagccgcc tgttcgagga gaattcggtt
    10261 tcggtgggcg cggattccgt tgacccggga tatcatgtgg ggtcccgcgc tcatgtagtt
    10321 tattcgggtt gagtagtctt gggcagctcc agccgcaagt cccatttgtg gctggtaact
    10381 ccacatgtag ggcgtgggaa tttccttgct cataatggcg ctgacgacag gtgctggcgc
    10441 cgggtgtggc cgctggagat gacgtagttt tcgcgcttaa atttgagaaa gggcgcgaaa
    10501 ctagtcctta agagtcagcg cgcagtattt gctgaagaga gcctccgcgt cttccagcgt
    10561 gcgccgaagc tgatcttcgc ttttgtgata caggcagctg cgggtgaggg agcgcagaga
    10621 cctgtttttt attttcagct cttgttcttg gcccctgctt tgttgaaata tagcatacag
    10681 agtgggaaaa atcctatttc taagctcgcg ggtcgatacg ggttcgttgg gcgccagacg
    10741 cagcgctcct cctcctgctg ctgccgccgc tgtggatttc ttgggctttg tcagagtctt
    10801 gctatccggt cgcctttgct tctgtgtgac cgctgctgtt gctgccgctg ccgctgccgc
    10861 cggtgcagta ggggctgtag agatgacggt agtaatgcag gatgttacgg gggaaggcca
    10921 cgccgtgatg gtagagaaga aagcggcggg cgaaggagat gttgccccca cagtcttgca
    10981 agcaagcaac tatggcgttc ttgtgcccgc gccacgagcg gtagccttgg cgctgttgtt
    11041 gctcttgggc taacggcggc ggctgcttag acttaccggc cctggttcca gtggtgtccc
    11101 atctacggtt gggtcggcga acaggcagtg ccggcggcgc ctgaggagcg gaggttgtag
    11161 cgatgctggg aacggttgcc aatttctggg gcgccggcga ggggaatgcg accgagggtg
    11221 acggtgtttc gtctgacacc tcttcggcct cggaagcttc gtctaggctg tcccagtctt
    11281 ccatcatctc ctcctcctcg tccaaaacct cctctgcctg actgtcccag tattcctcct
    11341 cgtccgtggg tggcggcggc ggcagctgca gcttcttttt gggtgccatc ctgggaagca
    11401 agggcccgcg gctgctgata gggctgcggc ggcgggggga ttgggttgag ctcctcgccg
    11461 gactgggggt ccaggtaaac cccccgtccc tttcgtagca gaaactcttg gcgggctttg
    11521 ttgatggctt gcaattggcc aaggatgtgg ccctgggtaa tgacgcaggc ggtaagctcc
    11581 gcatttggcg ggcgggattg gtcttcgtag aacctaatct cgtgggcgtg gtagtcctca
    11641 gggagaagga aatggccagt cgggaagagg agcagcgcga aaccaccccc gagcgcggac
    11701 gcggtgcggc gcgacgtcca ccaaccatgg aggacgtgtc gtccccgtcg ccgtcgccgc
    11761 cgcctccccg cgcgccccca aaaaagagac tgcggagacg cctggagtcc gaggacgagg
    11821 aagacagttc ccaggacgct ctggtgccta ggacaccatc tccaagaccc tccacttcca
    11881 ccgccgacct ggccattgcc agcaaaaaaa agaagaagag gccctcccct aaacctgaac
    11941 gaccacccag cccagaagtg atcgtggact cagaggaaga gcgcgaggac gtcgctctgc
    12001 agatggtggg attcagcaat ccacctgtgc tcatcaagca cggcaaaggg ggaaaacgga
    12061 ccgtgcgcag gctgaacgag gatgatcctg tggcaagggg catgaggaca caggaggaga
    12121 aggaagaatc ctccgaggcc gagagcgaga gcacagtgat taatccactg tccctgccaa
    12181 ttgtgagcgc ttgggagaag ggaatggagg ccgcccgggc cctgatggac aagtaccacg
    12241 tggacaacga cctgaaggct aatttcaaac tgctgcctga ccaggtggag gccctggctg
    12301 ccgtgtgcaa gacatggctg aatgaggagc atagagggct gcagctgacc tttacatcaa
    12361 acaagacctt tgtgaccatg atgggcaggt ttctgcaggc ctacctgcaa tctttcgccg
    12421 aagtgactta caagcaccac gagcccaccg gctgcgccct gtggctgcac agatgcgccg
    12481 agattgaggg cgagctgaaa tgcctgcacg gctctatcat gattaacaag gagcacgtga
    12541 tcgagatgga tgtgacttcc gaaaacggcc agcgcgccct gaaggagcag tccagcaagg
    12601 ccaagatcgt gaagaacagg tggggcagga acgtggtgca gatttctaac acagacgcca
    12661 gatgttgcgt gcatgacgcc gcctgccctg ccaatcagtt tagcggcaag agctgcggaa
    12721 tgttcttcag cgagggagcc aaggcgcaag tggccttcaa gcagatcaag gccttcatgc
    12781 aagccctgta tccaaatgcc cagaccggcc acggccacct cctgatgccc ctgcgctgcg
    12841 aatgtaactc caagcccggc cacgcccctt tcctgggcag gcagctgcca aaactgaccc
    12901 cttttgccct gagcaacgcc gaagatctgg atgccgatct gatcagcgac aagtccgtgc
    12961 tggcctccgt tcaccacccc gccctgatcg tgttccagtg ctgcaatccc gtgtacagga
    13021 atagcagagc tcagggtgga ggccccaact gcgacttcaa gatctccgcc ccagatctgc
    13081 tgaacgctct cgtgatggtg cggagcctgt ggtctgagaa cttcacagag ctgccccgca
    13141 tggtggtgcc cgagttcaag tggagcacta agcatcagta ccggaatgtg tctctgcccg
    13201 tggcccactc tgacgccaga cagaatcctt ttgacttctg aacggcgcag acggcaaggg
    13261 tggggggtaa ataatcaccc gagagtgtac aaataaaaac atttgccttt attgaaagtg
    13321 tctcctagta cattattttt acatgttttt caagtgacaa aaagaagtgg cgctcctaat
    13381 ctgcgcactg tggctgcgga agtagggcga gtggcgctcc aggaagctgt agagctgttc
    13441 ctggttgcga cgcagggtgg gctgtacctg gggactgtta agcatggagt tgggtaccgg
    13501 atccatcgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    13561 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    13621 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    13681 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    13741 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    13801 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    13861 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
    13921 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
    13981 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    14041 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgattgaa
    14101 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
    14161 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
    14221 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
    14281 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt actcgacgtt
    14341 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
    14401 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
    14461 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagaga
    14521 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
    14581 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
    14641 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
    14701 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
    14761 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
    14821 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
    14881 ttctgactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    14941 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    15001 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    15061 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    15121 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    15181 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    15241 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    15301 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    15361 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    15421 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    15481 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    15541 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    15601 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    15661 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    15721 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatca tatggtttaa
    15781 acgttggcag
//
