LOCUS       sequence-458367-       15817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV-PHP.eB 
            serotype, utilizing the AAVone single-plasmid AAV production 
            system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15817)
  AUTHORS   Yang R, Tran NT, Chen T, Cui M, Wang Y, Sharma T, Liu Y, Zhang J, 
            Yuan X, Zhang D, Chen C, Shi Z, Wang L, Dai Y, Zaidi H, Liang J, 
            Chen M, Jaijyan D, Hu H, Wang B, Xu C, Hu W, Gao G, Yu D, Tai PWL, 
            Wang Q
  TITLE     AAVone: A cost-effective, single-plasmid solution for efficient AAV 
            production with reduced DNA impurities.
  JOURNAL   Mol Ther Nucleic Acids. 2025 May 14;36(2):102563. doi: 
            10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10.
  PUBMED    40503174
REFERENCE   2  (bases 1 to 15817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2025-05-14"; volume: "36(2):102563. doi"; 
            pages: " 10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10"
COMMENT     Sequence Label: pAAVone-AAV-PHP.eB-CMV-eGFP
FEATURES             Location/Qualifiers
     source          1..15817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        134..512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     667..687
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          717..813
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      818..827
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(824..826,827..829,830..1543)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(824..826,827..829,830..1540)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(869..890)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1130..1149)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1477..1498
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1568..2156
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1621..1641)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2039..2050)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2188..2664
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2387..2406)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2673..2802
                     /label=AAV2 ITR
     CDS             complement(4050..4061)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     rep_origin      13534..13988
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13620..13639)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13830..13851
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14014..14118
                     /gene="bla"
                     /label=AmpR promoter
     CDS             14119..14913
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             14119..14913
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14173..14192)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14783..14802
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      15084..15672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15573..15592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcgtcgg gcgacctttg gtcgcccggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
      121 cggccgcacg cgtacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      181 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc acctgcgtaa
      721 gtttagtctt tttgtctttt atttcaggtc ccggatccgg tggtggtgca aatcaaagaa
      781 ctgctcctca gtggatgttg cctttacttc taggcgcgcc accatggtga gcaagggcga
      841 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      901 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      961 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1021 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1081 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1141 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1201 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1261 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1321 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1381 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1441 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1501 cgccgccggg atcactctcg gcatggacga gctgtacaag taacgtacgg atatcaagct
     1561 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1621 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1681 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1741 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1801 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1861 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1921 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     1981 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2041 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2101 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2161 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     2221 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2281 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     2341 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     2401 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     2461 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     2521 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     2581 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     2641 accactgctc ccttccctgt ccttttaatt aaaggaaccc ctagtgatgg agttggccac
     2701 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     2761 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgccaac cggtgtcgac
     2821 gtaatccgta gatgtacctg gacatccagg tgatgccggc ggcggtggtg gaggcgcgcg
     2881 gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa aaagtgctcc atggtcggga
     2941 cgctctggcc ggtgaggcgt gcgcagtcgt tgacgctcta gaccgtgcaa aaggagagcc
     3001 tgtaagcggg cactcttccg tggtctggtg gataaattcg caagggtatc atggcggacg
     3061 accggggttc gaaccccgga tccggccgtc cgccgtgatc catgcggtta ccgcccgcgt
     3121 gtcgaaccca ggtgtgcgac gtcagacaac gggggagcgc tccttttggc ttccttccag
     3181 gcgcggcggc tgctgcgcta gcttttttgg ccactggccg cgcgcggcgt aagcggttag
     3241 gctggaaagc gaaagcatta agtggctcgc tccctgtagc cggagggtta ttttccaagg
     3301 gttgagtcgc aggacccccg gttcgagtct cgggccggcc ggactgcggc gaacgggggt
     3361 ttgcctcccc gtcatgcaag accccgcttg caaattcctc cggaaacagg gacgagcccc
     3421 ttttttgctt ttcccagatg catccggtgc tgcggcagat gcgcccccct cctcagcagc
     3481 ggcaagagca agagcagcgg cagacatgca gggcaccctc cccttctcct accgcgtcag
     3541 gaggggcaac atcctacatc gatagatctg cagaattcgg cttggcggct gcgcgttcaa
     3601 acctcccgct tcaaaatgga gaccctgcgt gctcactcgg gcttaaatac ccagcgtgac
     3661 cacatggtgt cgcaaaatgt cgcaaaacac tcacgtgacc tctaatacag gacctctaga
     3721 gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaaag ccgaattcca
     3781 gcacactggc ggccgttact agtggatcct agagcatgga aactagataa gaaagaaata
     3841 cgcagagacc aaagttcaac tgaaacgaat taaacggttt attgattaac aagcgatcgt
     3901 tattacagat tacgagtcag gtatctggtg ccaatggggc ggggttcact atatacacct
     3961 tcagtattaa cagcaaattc aacattatta gacttgtaat agttggaagt gtactggatc
     4021 tccgggttcc agcgcttgct gttttccttc tgcagctccc actcgatctc cacgctgact
     4081 tggccagtag aatactgggt gatgaaagag ttcagcttgt ccttgttgaa ggccgttgga
     4141 ggatccgcag gtacaggtgt gtttttgatg aggatctgag gaggcgggtg cttcattcca
     4201 aaccctccca tcagcggaga agggtgaaag ttgccgtccg tgtgaggaat tttggcccaa
     4261 atgggtcctt gcaggtacac atctctgtcc tgccaaacca tacccggaag tattccttgg
     4321 ttttgaaccc agccggtctg cgcctgtgcc ttaaaaggca ccgccaaagt cccatcactc
     4381 tggtggtttg tggccacttg tccataggac tccgttgcta ccgggttagt agttttaatt
     4441 tcttcttcgt tggttatcat gactttgtcc gcatccacgt tgtctcttcc agttccttgt
     4501 ttgccaaaaa ttaaagatcc agacaaagga aagaaacggt cctctccttc tttgtggctg
     4561 gccatagcag gtccaggatt catcaagcta ttacgtccat tgagagccca agaagaagct
     4621 ccaggccaag caaattcgct gttgttgttt tgagtcacag tggttgagac acgttgttgt
     4681 cggtagctgg gtccaggtat gtagtttctt ccctggacag ccatgttgct gggtccggcc
     4741 acactgaatt ttagcgtttg ttgattctgt ccagaaccgt taatagtctt tgagagatag
     4801 tacaagtatt ggtcgatgag tggattcatt agtcggtcca ggctttggct gtgagcgtag
     4861 ctgctatgga aaggtacgtt ctcaaactcg tagctgaact ggaagttgtt acccgttctt
     4921 agcatttgcg acgggaaata ttccaggcag taaaaggacg aacgacccac ggcctggctt
     4981 ccatcattaa gcgtcagata cccgtactga ggaatcatga aaacgtccgc tgggaacggc
     5041 gggaggcagc cctcgtgagc cgacccgagc acgtacggga gctgatagtc tgagtccgtg
     5101 aagacctgga ccgtgctggt aaggttattg gcgatggtct tgactccatt gttgtccgta
     5161 acctctttga cctgaatgtt gaagagcttg aagttgagtc gcttaggccg gaatccccag
     5221 ttgttgttga tgagtcgctg ccagtcacgt ggtgagaagt ggcagtggaa tctgttgaag
     5281 tcaaaatacc cccagggggt gctgtagccg aagtaggcgt tgtcatttga agatcctcca
     5341 gatgtgctgt tggagatttg cttgtagagg tgattgttgt aggtgggcag ggcccaggtt
     5401 cgggtgctgg tggtgatgac tctgtccccc agccattggg aatcgcaatg ccaatttccc
     5461 gaggaactac ccactccatc ggcaccttcg ttattgtctg ccactggtgc gccaccacct
     5521 gaagccattg taagagatcc cacacctgag ggggctgcgg gaggttctcc gattggttga
     5581 gggtctggga ctgactctgt gtcgccagtc tgaccgaaat tgagtctctt tttagcgggc
     5641 tgtgcacccg atttgccaat acccgcggag gagtccggtt cctgaggaga ctgctctaca
     5701 ggcctcttct ttccaggagc cgtcttagcc gcttcctcaa ccagaccaag aggttcaaga
     5761 agcctctttt tggcctggaa gactgctcgc ccgaggttgc ccccaaaaga cgtatcttct
     5821 ttgagccgct cctggaactc ggcgtcggcg tggttgtact tgaggtacgg gttgtctccg
     5881 gccttgagct gctggtcgta ggccttgtcg tgctcgaggg ccgccgcgtc tgctgcgttg
     5941 accggctccc ccttgtcgag tccgttgccg ggtccaaggt atttgtaacc cggaagcaca
     6001 agacctcgag cgttgtcttg atgttgttga tttgccttgg gttgaggggc tccaggtttc
     6061 aaagcccacc actcgcgaat tccttcacta aggttgtcct cgagccaatc tggaagataa
     6121 ccatcggcag ccatacctga tttaaatcat ttattgttca aagatgcagt catccaaatc
     6181 cacattgacc agatcgcagg cagtgcaagc gtctggcacc tttcccatga tatgatgaat
     6241 gtagcacagt ttctgatacg cctttttgac gacagaaacg ggttgagatt ctgacacggg
     6301 aaagcactct aaacagtctt tctgtccgtg agtgaagcag atatttgaat tctgattcat
     6361 tctctcgcat tgtctgcagg gaaacagcat cagattcatg cccacgtgac gagaacattt
     6421 gtttacgtac ctgtctgcgt agttgatcga agcttccgcg tctgacgtcg atggctgcgc
     6481 aactgactcg cgcacccgtt tgggctcact tatatctgcg tcactggggg cgggtctttt
     6541 cttggctcca ccctttttga cgtagaattc atgctccacc tcaaccacgt gatcctttgc
     6601 ccaccggaaa aagtctttga cttcctgctt ggtgaccttc ccaaagtcat gatccagacg
     6661 gcgggtgagt tcaaatttga acatccggtc ttgcaacggc tgctggtgtt cgaaggtcgt
     6721 tgagttcccg tcaatcacgg cgcacatgtt ggtgttggag gtgacgatca cgggagtcgg
     6781 gtctatctgg gccgaggact tgcatttctg gtccacgcgc accttgcttc ctccgagaat
     6841 ggctttggcc gactccacga ccttggcggt catcttcccc tcctcccacc agatcaccat
     6901 cttgtcgaca cagtcgttga agggaaagtt ctcattggtc cagtttacgc acccgtagaa
     6961 gggcacagtg tgggctatgg cctccgcgat gttggtcttc ccggtagttg caggcccaaa
     7021 cagccagatg gtgttcctct tgccgaactt tttcgtggcc catcccagaa agacggaagc
     7081 cgcatattgg ggatcgtacc cgtttagttc caaaatttta taaatccgat tgctggaaat
     7141 gtcctccacg ggctgctggc ccaccaggta gtcgggggcg gttttagtca ggctcataat
     7201 ctttcccgca ttgtccaagg cagccttgat ttgggaccgc gagttggagg ccgcattgaa
     7261 ggagatgtat gaggcctggt cctcctggat ccactgcttc tccgaggtaa tccccttgtc
     7321 cacgagccac ccgaccagct ccatgtacct ggctgaagtt tttgatctga tcaccggcgc
     7381 atcagaattg ggattctgat tctctttgtt ctgctcctgc gtctgcgaca cgtgcgtcag
     7441 atgctgcgcc accaaccgtt tacgctccgt gagattcaaa caggcgctta aatactgttc
     7501 catattagtc cacgcccact ggagctcagg ctgggttttg gggagcaagt aattggggat
     7561 gtagcactca tccaccacct tgttcccgcc tccggcgcca tttctggtct ttgtgaccgc
     7621 gaaccagttt ggcaaagtcg gctcgatccc gcggtaaatt ctctgaatca gtttttcgcg
     7681 aatctgactc aggaaacgtc ccaaaaccat ggatttcacc ccggtggttt ccacgagcac
     7741 gtgcatgtgg aagtagctct ctcccttctc aaattgcaca aagaaaagag cctccggtgc
     7801 cttactcaca cggcgccatt ccgtcagaaa gtcgcgctgc agcttctcgg ccacggtcag
     7861 gggtgcctgc tcaatcagat tcagatccat gtcagaatct ggcggcaact cccattcctt
     7921 ctcggccacc cagttcacaa agctgtcaga aatgccgggc agatgctcgt caaggtcgct
     7981 ggggacctta atcacaatct cgtaaaaccc cggcatggcg gctgctcgtt caaatttcgc
     8041 ggtcaaaatg gaggttgcat cctgctcgct cactcggcca tatatacctc agcgtgacca
     8101 catggtgtcg caaaatgtcg caaaacactc acgttgacct ctaatacagg accgcggccg
     8161 ctctagtgaa tccacagaaa ctagcgaggt aagcacttac tctatgtctt ttacatggtc
     8221 ctgggaaagt ggaaaataca ccactgaaac ttttgctacc aactcttaca ccttctccta
     8281 cattgcccag gaataaagaa tcgtgaacct gttgcatgtt atgtttcaac gtgtttattt
     8341 ttcaattgca gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata
     8401 gcttatattg atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac
     8461 ctccctccca acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca
     8521 tatcatgggt aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca
     8581 aacgctcatc agtgatatta ataaactccc cgggcagctc gcttaagttc atgtcgctgt
     8641 ccagctgctg agccacaggc tgctgtccaa cttgcggttg ctcaacgggc ggcgaagggg
     8701 aagtccatgc ctacatgggg gtagagccat aatcgtgcat caggataggg cggtggtgct
     8761 gcagcagcgc gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg
     8821 cagtggtctc ctcagcgatg attcgcaccg cccgcagcat gagacgcctt gtcctccggg
     8881 cacagcagcg caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa
     8941 tattgttcaa aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag
     9001 aacccacgtg gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca
     9061 cgctggacat aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata
     9121 taaacctctg attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct
     9181 gcccgccggc tatgcactgc agggaaccgg gactggaaca atgacagtgg agagcccagg
     9241 actcgtaacc atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca
     9301 cgtgcataca cttcctcagg attacaagct cctcccgcgt cagaaccata tcccagggaa
     9361 caacccattc ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca
     9421 cgttgtgcat tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag
     9481 cgcgggtctc tgtctcaaaa ggaggtaggc gatccctact gtacggagtg cgccgagaca
     9541 accgagatcg tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc
     9601 ctgaagcaaa accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgtcgctta
     9661 gctcgctctg tgtagtagtt gtagtatatc cactctctca aagcatccag gcgcctaggc
     9721 aaaatagcac cctcccgctc cagaacaaca tacagcgctt ccacagcggc agccataaca
     9781 gtcagcctta ccagtaaaaa aacctattaa aaaacaccac tcgacacggc accagctcaa
     9841 tcagtcacag tgtaaaaagg gccaagtaca gagcgagtat atataggact aaaaaatgac
     9901 gtaacggtta aagtccacaa aaaacaccca gaaaaccgca cgcgaaccta cgcccagaaa
     9961 cgaaagccaa aaaacccaca acttcctcaa atcttcactt ccgttttccc acgatacgtc
    10021 acttcccatt ttaaaaaaac tacaattccc aatacatgca agttactccg ccctaaaacg
    10081 cccgggcgac cgcaccctgt gacgaaagcc gcccgcaagc tgcgcccctg agttagtcat
    10141 ctgaacttcg gcctgggcgt ctctgggaag taccacagtg gtgggagcgg gactttcctg
    10201 gtacaccagg gcagcgggcc aactacgggg attaaggtta ttacgaggtg tggtggtaat
    10261 agccgcctgt tcgaggagaa ttcggtttcg gtgggcgcgg attccgttga cccgggatat
    10321 catgtggggt cccgcgctca tgtagtttat tcgggttgag tagtcttggg cagctccagc
    10381 cgcaagtccc atttgtggct ggtaactcca catgtagggc gtgggaattt ccttgctcat
    10441 aatggcgctg acgacaggtg ctggcgccgg gtgtggccgc tggagatgac gtagttttcg
    10501 cgcttaaatt tgagaaaggg cgcgaaacta gtccttaaga gtcagcgcgc agtatttgct
    10561 gaagagagcc tccgcgtctt ccagcgtgcg ccgaagctga tcttcgcttt tgtgatacag
    10621 gcagctgcgg gtgagggagc gcagagacct gttttttatt ttcagctctt gttcttggcc
    10681 cctgctttgt tgaaatatag catacagagt gggaaaaatc ctatttctaa gctcgcgggt
    10741 cgatacgggt tcgttgggcg ccagacgcag cgctcctcct cctgctgctg ccgccgctgt
    10801 ggatttcttg ggctttgtca gagtcttgct atccggtcgc ctttgcttct gtgtgaccgc
    10861 tgctgttgct gccgctgccg ctgccgccgg tgcagtaggg gctgtagaga tgacggtagt
    10921 aatgcaggat gttacggggg aaggccacgc cgtgatggta gagaagaaag cggcgggcga
    10981 aggagatgtt gcccccacag tcttgcaagc aagcaactat ggcgttcttg tgcccgcgcc
    11041 acgagcggta gccttggcgc tgttgttgct cttgggctaa cggcggcggc tgcttagact
    11101 taccggccct ggttccagtg gtgtcccatc tacggttggg tcggcgaaca ggcagtgccg
    11161 gcggcgcctg aggagcggag gttgtagcga tgctgggaac ggttgccaat ttctggggcg
    11221 ccggcgaggg gaatgcgacc gagggtgacg gtgtttcgtc tgacacctct tcggcctcgg
    11281 aagcttcgtc taggctgtcc cagtcttcca tcatctcctc ctcctcgtcc aaaacctcct
    11341 ctgcctgact gtcccagtat tcctcctcgt ccgtgggtgg cggcggcggc agctgcagct
    11401 tctttttggg tgccatcctg ggaagcaagg gcccgcggct gctgataggg ctgcggcggc
    11461 ggggggattg ggttgagctc ctcgccggac tgggggtcca ggtaaacccc ccgtcccttt
    11521 cgtagcagaa actcttggcg ggctttgttg atggcttgca attggccaag gatgtggccc
    11581 tgggtaatga cgcaggcggt aagctccgca tttggcgggc gggattggtc ttcgtagaac
    11641 ctaatctcgt gggcgtggta gtcctcaggg agaaggaaat ggccagtcgg gaagaggagc
    11701 agcgcgaaac cacccccgag cgcggacgcg gtgcggcgcg acgtccacca accatggagg
    11761 acgtgtcgtc cccgtcgccg tcgccgccgc ctccccgcgc gcccccaaaa aagagactgc
    11821 ggagacgcct ggagtccgag gacgaggaag acagttccca ggacgctctg gtgcctagga
    11881 caccatctcc aagaccctcc acttccaccg ccgacctggc cattgccagc aaaaaaaaga
    11941 agaagaggcc ctcccctaaa cctgaacgac cacccagccc agaagtgatc gtggactcag
    12001 aggaagagcg cgaggacgtc gctctgcaga tggtgggatt cagcaatcca cctgtgctca
    12061 tcaagcacgg caaaggggga aaacggaccg tgcgcaggct gaacgaggat gatcctgtgg
    12121 caaggggcat gaggacacag gaggagaagg aagaatcctc cgaggccgag agcgagagca
    12181 cagtgattaa tccactgtcc ctgccaattg tgagcgcttg ggagaaggga atggaggccg
    12241 cccgggccct gatggacaag taccacgtgg acaacgacct gaaggctaat ttcaaactgc
    12301 tgcctgacca ggtggaggcc ctggctgccg tgtgcaagac atggctgaat gaggagcata
    12361 gagggctgca gctgaccttt acatcaaaca agacctttgt gaccatgatg ggcaggtttc
    12421 tgcaggccta cctgcaatct ttcgccgaag tgacttacaa gcaccacgag cccaccggct
    12481 gcgccctgtg gctgcacaga tgcgccgaga ttgagggcga gctgaaatgc ctgcacggct
    12541 ctatcatgat taacaaggag cacgtgatcg agatggatgt gacttccgaa aacggccagc
    12601 gcgccctgaa ggagcagtcc agcaaggcca agatcgtgaa gaacaggtgg ggcaggaacg
    12661 tggtgcagat ttctaacaca gacgccagat gttgcgtgca tgacgccgcc tgccctgcca
    12721 atcagtttag cggcaagagc tgcggaatgt tcttcagcga gggagccaag gcgcaagtgg
    12781 ccttcaagca gatcaaggcc ttcatgcaag ccctgtatcc aaatgcccag accggccacg
    12841 gccacctcct gatgcccctg cgctgcgaat gtaactccaa gcccggccac gcccctttcc
    12901 tgggcaggca gctgccaaaa ctgacccctt ttgccctgag caacgccgaa gatctggatg
    12961 ccgatctgat cagcgacaag tccgtgctgg cctccgttca ccaccccgcc ctgatcgtgt
    13021 tccagtgctg caatcccgtg tacaggaata gcagagctca gggtggaggc cccaactgcg
    13081 acttcaagat ctccgcccca gatctgctga acgctctcgt gatggtgcgg agcctgtggt
    13141 ctgagaactt cacagagctg ccccgcatgg tggtgcccga gttcaagtgg agcactaagc
    13201 atcagtaccg gaatgtgtct ctgcccgtgg cccactctga cgccagacag aatccttttg
    13261 acttctgaac ggcgcagacg gcaagggtgg ggggtaaata atcacccgag agtgtacaaa
    13321 taaaaacatt tgcctttatt gaaagtgtct cctagtacat tatttttaca tgtttttcaa
    13381 gtgacaaaaa gaagtggcgc tcctaatctg cgcactgtgg ctgcggaagt agggcgagtg
    13441 gcgctccagg aagctgtaga gctgttcctg gttgcgacgc agggtgggct gtacctgggg
    13501 actgttaagc atggagttgg gtaccggatc catcgcgccc tgtagcggcg cattaagcgc
    13561 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    13621 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    13681 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    13741 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    13801 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    13861 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    13921 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
    13981 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
    14041 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    14101 tattgaaaaa ggaagagtat gattgaacaa gatggattgc acgcaggttc tccggccgct
    14161 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
    14221 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
    14281 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
    14341 gttccttgcg cagctgtact cgacgttgtc actgaagcgg gaagggactg gctgctattg
    14401 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
    14461 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
    14521 caccaagcga aacatcgcat cgagagagca cgtactcgga tggaagccgg tcttgtcgat
    14581 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
    14641 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
    14701 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
    14761 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
    14821 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
    14881 gccttctatc gccttcttga cgagttcttc tgactgtcag accaagttta ctcatatata
    14941 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    15001 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    15061 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    15121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    15181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    15241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    15301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    15361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    15421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    15481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    15541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    15601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    15661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    15721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    15781 tttgagtgag ctgatcatat ggtttaaacg ttggcag
//
