LOCUS       sequence-458376-        6611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human SYK Kinase in insect cells (SF9 cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6611)
  TITLE     Mesecar lab plasmids 2024
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFastBacHTa_SYK
FEATURES             Location/Qualifiers
     source          1..6611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..31
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..73
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2072..2206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2109..2128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2163..2182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2235..2400
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2584..3038
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2671..2690)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2880..2901
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3064..3168
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3169..3237,3238..4029)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3387..3406)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4200..4788
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4689..4708
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4942..4959
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5093..5317
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5384..5917)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6087..6092,6093..6109,6110..6115))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        6467..6558
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     6483..6500
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 catgtcgtac taccatcacc atcaccatca cgattacgat atcccaacga ccgaaaacct
       61 gtattttcag ggcgccatgg atccgatggc aagttctggt atggccgaca gcgcaaatca
      121 cctgccattt tttttcggga atatcactcg cgaggaagca gaagactacc tagtacaggg
      181 ggggatgtcc gacgggttat atctactgcg acagtcacgc aactacttag gcgggtttgc
      241 tcttagtgtc gcccatggtc ggaaagccca ccattacact attgaaaggg agttaaacgg
      301 tacgtacgct atcgccggag gacgtacaca cgcgagtccg gcggatctgt gccattatca
      361 ctctcaagag tcagatggac tagtatgctt actcaaaaag ccgttcaacc gaccgcaggg
      421 agttcaaccg aagactggcc cgttcgagga tttgaaggag aacctaatcc gagaatatgt
      481 gaagcaaact tggaatctac aaggccaggc cttagagcaa gccattattt cacaaaagcc
      541 acagcttgag aagttgatag ccaccacggc tcacgaaaaa atgccatggt tccatggcaa
      601 aatttcccgc gaagaatcgg aacaaatagt gctcattggg tcgaagacca acggcaaatt
      661 tcttataagg gctcgggaca ataatggatc atatgcactt tgcttactgc atgaagggaa
      721 ggttctacat tatagaatag ataaagacaa aactggcaag ctctcaatcc ctgagggtaa
      781 gaaattcgac acgctttggc aactcgttga acactacagc tataaagcgg acggattact
      841 gagagtcctt actgtgccat gccagaagat cggaacacag ggcaacgtta actttggggg
      901 ccgtccccaa ttgcccggct cgcacccagc gtcctctccc gcacagggaa ataggcagga
      961 atcgaccgtt agctttaacc cgtatgaacc tgagttggca ccttgggccg ctgataaagg
     1021 gccacaacgc gaagcgcttc cgatggatac agaagtatat gagtctccgt acgctgatcc
     1081 cgaagagatc cggcctaaag aagtgtatct cgaccgtaaa ctactgacgt tggaggataa
     1141 ggaattaggg tccgggaatt ttggtaccgt gaagaaggga tattaccaaa tgaaaaaggt
     1201 agtgaaaaca gtagcagtaa aaatactgaa aaatgaggct aacgatccag ctttgaagga
     1261 tgagctccta gccgaagcga atgtcatgca gcaattggac aatccataca ttgtcaggat
     1321 gataggaata tgtgaagcgg agtcttggat gcttgtcatg gaaatggctg aattaggtcc
     1381 cctaaataaa tatctccagc aaaaccgtca cgtgaaggat aagaatataa tcgagttggt
     1441 tcatcaggta tctatgggta tgaaatacct agaggagagc aatttcgtcc atcgagacct
     1501 cgctgccaga aatgttctgc tcgtgacgca acactatgcg aagatttcgg actttggact
     1561 tagtaaggcg ttacgcgcag atgaaaacta ctacaaagcg cagacacatg gtaaatggcc
     1621 cgtaaagtgg tatgcccctg agtgtatcaa ctattacaaa ttcagctcga aaagtgacgt
     1681 ctggagtttc ggtgtgttga tgtgggaggc gttttcctac ggccagaaac cttaccgggg
     1741 catgaagggt tcagaggtca ccgcgatgtt agaaaaaggc gaacggatgg gttgtcccgc
     1801 aggttgtcct agagagatgt atgatctgat gaacttgtgc tggacatatg acgtcgagaa
     1861 cagacccggc ttcgcagctg tagagcttcg attgcggaat tattactacg atgttgttaa
     1921 ctgactcgag gcatgcggta ccaagcttgt cgagaagtac tagaggatca taatcagcca
     1981 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     2041 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     2101 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2161 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga
     2221 gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa
     2281 ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa
     2341 ataatcctta aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca
     2401 gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact ccatgtgaca
     2461 aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct gtcatctctt
     2521 cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg aatggcgaat
     2581 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2641 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2701 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2761 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2821 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag
     2881 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     2941 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3001 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     3061 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3121 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3181 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3241 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3301 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3361 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3421 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3481 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3541 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3601 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3661 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3721 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3781 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3841 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     3901 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3961 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4021 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4081 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4141 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4201 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4261 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4321 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4381 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4441 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4501 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4561 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4621 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4681 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4741 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4801 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4861 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4921 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     4981 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca gccgcgtaac
     5041 ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg
     5101 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
     5161 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
     5221 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
     5281 gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac aatttaccga
     5341 acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     5401 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     5461 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     5521 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     5581 ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag
     5641 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     5701 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     5761 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     5821 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     5881 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     5941 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     6001 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     6061 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     6121 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     6181 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc
     6241 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgccctggc
     6301 ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg
     6361 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta
     6421 gctatagttc tagtggttgg ctacgtatac tccggaatat taatagatca tggagataat
     6481 taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta
     6541 ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatct
     6601 cggtccgaaa c
//
