LOCUS       sequence-458379-        6125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human LYN Kinase in insect cells (SF9 cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6125)
  TITLE     Mesecar lab plasmids 2024
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFastBacHTa_LYN
FEATURES             Location/Qualifiers
     source          1..6125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..31
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..73
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    1586..1720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1623..1642)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1677..1696
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1749..1914
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2098..2552
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2185..2204)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2394..2415
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2578..2682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2683..2751,2752..3543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2901..2920)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3714..4302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4203..4222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4456..4473
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4607..4831
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4898..5431)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5601..5606,5607..5623,5624..5629))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5981..6072
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5997..6014
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 catgtcgtac taccatcacc atcaccatca cgattacgat atcccaacga ccgaaaacct
       61 gtattttcag ggcgccatgg atccggaaca gggggatatc gttgtggcgt tgtacccata
      121 tgatggcatc catcccgatg atctcagctt caagaaaggc gagaaaatga aggtcttaga
      181 agaacacggc gagtggtgga aggccaagtc gcttttgact aagaaggaag gattcatacc
      241 atccaactat gtggcgaaac taaacacact ggaaacagag gaatggttct tcaaggatat
      301 cactcgcaaa gacgccgagc gtcagcttct tgcgcctggt aattccgctg gcgcttttct
      361 gatacgggaa tctgagactc tcaaaggtag cttttcactg agtgttaggg acttcgaccc
      421 tgtccacggc gacgtcatca aacattacaa aattagatct ttagacaatg ggggttatta
      481 tatctctccc cgaatcacgt tcccgtgtat ctcagatatg atcaaacact atcagaagca
      541 ggcagatgga ttatgtcgcc gattggaaaa agcctgcata tcgccgaaac cccaaaaacc
      601 gtgggacaaa gacgcatggg aaataccacg agaaagcatc aaactagtca agcggctcgg
      661 ggccggacaa tttggcgaag tgtggatggg atactacaac aattctacca aggtcgcagt
      721 caaaacactc aagcccggaa ccatgagtgt acaagccttt ctagaggaag cgaatctgat
      781 gaaaacgctg caacatgaca agctagttag attgtacgct gttgtgacca gggaggagcc
      841 catttatatt attactgagt atatggcgaa gggaagtttg cttgattttc taaaatccga
      901 cgagggtggc aaggttttat tgccaaagct aattgacttc tcggctcaaa ttgccgaagg
      961 tatggcttac atagagcgta agaactacat acaccgagac ctccgcgcag caaacgtatt
     1021 agtatccgaa agcttgatgt gcaaaattgc ggattttggg ctggctcgtg tgattgaaga
     1081 taacgaatac acggcacggg aaggggctaa atttcctatt aaatggaccg caccggaggc
     1141 gataaatttt ggatgtttta ccataaagag tgacgtatgg tcgttcggta tactattata
     1201 tgagattgtt acatatggga agatacccta ccctggtcgg acgaatgccg acgtaatgac
     1261 agcgctttca caaggatata ggatgccaag agtagagaac tgcccggatg agctgtacga
     1321 catcatgaag atgtgctgga aagaaaaggc tgaggagagg ccaacttttg attaccttca
     1381 gtcagtgctc gatgatttct atacggccac agaggggcag taccagcaac aaccttgact
     1441 cgaggcatgc ggtaccaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     1501 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     1561 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     1621 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     1681 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
     1741 gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
     1801 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
     1861 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg
     1921 catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt
     1981 catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat
     2041 taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc gaatgggacg
     2101 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2161 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2221 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2281 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     2341 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttctta atagtggact
     2401 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     2461 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2521 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     2581 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2641 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2701 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2761 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2821 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2881 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2941 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3001 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3061 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3121 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3181 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3241 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3301 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3361 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3421 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3481 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3541 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3601 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3661 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3721 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3781 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3841 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3901 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3961 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4021 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4081 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4141 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4201 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4261 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4321 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4381 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4441 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     4501 tttctcctta cgcatctgtg cggtatttca caccgcatag accagccgcg taacctggca
     4561 aaatcggtta cggttgagta ataaatggat gccctgcgta agcgggtgtg ggcggacaat
     4621 aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta aactagacag
     4681 aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg gacttttgtt
     4741 atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat taaagagggg
     4801 cgtggccaag ggcatggtaa agactatatt cgcggcgttg tgacaattta ccgaacaact
     4861 ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga
     4921 tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat
     4981 cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct
     5041 tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga
     5101 gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg taagccgcga
     5161 gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga
     5221 gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga
     5281 actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc
     5341 tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct
     5401 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
     5461 gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa gccatgaaaa
     5521 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
     5581 gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg
     5641 ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg
     5701 catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat
     5761 tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg
     5821 agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg
     5881 ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccaggac tctagctata
     5941 gttctagtgg ttggctacgt atactccgga atattaatag atcatggaga taattaaaat
     6001 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     6061 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctcggtcc
     6121 gaaac
//
