LOCUS       sequence-458423-       10699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA cloning vector with direct capture sequence 1 in stem loop 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Liu S, Hamilton MC, Cowart T, Barrera A, Bounds LR, Nelson AC, 
            Dornbaum SF, Riley JW, Doty RW, Allen AS, Crawford GE, Majoros WH, 
            Gersbach CA
  TITLE     Characterization and bioinformatic filtering of ambient gRNAs in 
            single-cell CRISPR screens using CLEANSER.
  JOURNAL   Cell Genom. 2025 Feb 4:100766. doi: 10.1016/j.xgen.2025.100766.
  PUBMED    39914388
REFERENCE   2  (bases 1 to 10699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Genom.
            2025 Feb 4:100766. doi: 10.1016/j.xgen.2025.100766."
COMMENT     Sequence Label: pLenti-hU6-cs1-PuroR-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..10699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2620..2868
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2620..2640
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2791..2810
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        4938..5149
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5150..6088
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6045..6065
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6109..6705
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6109..6128)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6605..6625
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6715..6768
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6781..7479
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(7499..7532)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7588..8176
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7641..7661)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8059..8070)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8179..8195)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8180..8196)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8705..8885
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8947..9535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9027..9046)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9706..10497,10498..10566))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10329..10348
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10567..10671)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag agggcctatt tcccatgatt
     2641 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
     2701 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
     2761 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
     2821 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg agacggttgt
     2881 aaatgagcac acaaaataca catgctaaaa tattatattc tatgaccttt ataaaatcaa
     2941 ccaaaatctt ctttttaata actttagtat caataattag aatttttatg ttcctttttg
     3001 caaactttta ataaaaatga gcaaaataaa aaaacgctag ttttagtaac tcgcgttgtt
     3061 ttcttcacct ttaataatag ctactccacc acttgttcct aagcggtcag ctcctgcttc
     3121 aatcattttt tgagcatctt caaatgttct aactccacca gctgctttaa ctaaagcatt
     3181 gtctttaaca actgacttca ttagtttaac atcttcaaat gttgcacctg attttgaaaa
     3241 tcctgttgat gttttaacaa attctaatcc agcttcaaca gctatttcac aagctttcat
     3301 gatttcttct tttgttaata aacaattttc cataatacat ttaacaacat gtgatccagc
     3361 tgcttttttt acagctttca tgtcttctaa aactaattca taatttttgt cttttaatgc
     3421 accaatattt aataccatat caatttctgt tgcaccatct ttaattgctt cagaaacttc
     3481 gaatgctttt gtagctgttg tgcatgcacc tagaggaaaa cctacaacat ttgttattcc
     3541 tacatttgtg ccttttaata attctttaca atagcttgtt caatatgaat taacacaaac
     3601 tgttgcaaaa tcaaattcaa ttgcttcatc acataattgt ttaatttcag ctttcgtagc
     3661 atcttgtttt aataatgtgt gatctatata tttgtttagt ttcatttttt ctcctatata
     3721 ttcattttta attttaattc tttaataatt tcgtctactt taactttagc gttttgaaca
     3781 gattcaccaa cacctataaa ataaattttt agtttaggtt cagttccact tgggcgaaca
     3841 gcaaatcatg acttatcttc taaataaaat tttagtaagt cttgtcctgg catattatac
     3901 attccatcga tgtagtcttc aacattaaca actttaagtc cagcaatttg agttaagggt
     3961 gttgctctca atgatttcat taatggttca atttttaatt tcttttcttc tggtttaaaa
     4021 ttcaagttta aagtgaaagt gtaatatgca cccatttctt taaataaatc ttctaaatag
     4081 tctactaatg ttttattttg ttttttataa aatcaagcag cctctgctat taatatagaa
     4141 gcttgtattc catctttatc tctagctgag tcatcaatta catatccata actttcttca
     4201 taagcaaaaa caaaatttaa tccgttatct tcttctttag caatttctct acccattcat
     4261 ttaaatccag ttaaagtttt tacaatatta actccatatt tttcatgagc gattctatca
     4321 cccaaatcac ttgttacaaa acttgaatat agagccggat tttttggaat gctatttaag
     4381 cgttttagat ttgataattt tcaatcaatt aaaattggtc ctgtttgatt tccatctaat
     4441 cttacaaaat gaccatcatg ttttattgcc attccaaatc tgtcagcatc tgggtcattc
     4501 ataataataa tatctgcatc atgtttaata ccatattcaa gcggtatttt tcatgcagga
     4561 tcaaattctg gatttggatt tacaacattt ttaaatgttt catcttcaaa tgcatgctct
     4621 tcaacctcaa taacgttata tcctgattca cgtaatattt ttggggtaaa tttagttcct
     4681 gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg ctcttttttg
     4741 tacgtctctg tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc
     4801 gttatcaact tggccgcttt aaggccggtc ctagcaaggc caagtggcac cgagtcggtg
     4861 ctttttttct cgagtactag gatccattag gcggccgcgt ggataaccgt attaccgcca
     4921 tgcatgtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     4981 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     5041 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     5101 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     5161 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     5221 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     5281 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     5341 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     5401 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     5461 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     5521 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     5581 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     5641 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     5701 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     5761 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     5821 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     5881 gttctcgcgc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     5941 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     6001 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     6061 ggttcaaagt ttttttcttc catttcaggt gtcgtgagct agcccaccat gaccgagtac
     6121 aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc
     6181 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag
     6241 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6301 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6361 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6421 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6481 ctggccaccg tcggcgtgtc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6541 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6601 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6661 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc gggagagggc
     6721 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccacc ggtcgccacc
     6781 agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac
     6841 catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
     6901 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc
     6961 ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag
     7021 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
     7081 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
     7141 agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag
     7201 gccttcaccg agaccctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc
     7261 ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag
     7321 aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga
     7381 atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac
     7441 tgcgacctcc ctagcaaact ggggcacaag cttaattaag aattcgtcga gggacctaat
     7501 aacttcgtat agcatacatt atacgaagtt atacatgttt aagggttccg gttccactag
     7561 gtacaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     7621 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7681 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7741 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7801 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7861 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7921 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7981 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8041 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8101 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8161 tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc
     8221 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     8281 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     8341 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8401 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     8461 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     8521 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     8581 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     8641 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     8701 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8761 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8821 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8881 tagcagcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8941 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9001 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9061 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9121 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9181 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9241 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9301 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9361 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9421 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9481 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9541 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9601 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9661 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9721 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9781 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9841 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9901 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9961 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10021 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10081 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10141 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10201 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10261 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10321 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10381 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10441 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10501 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10561 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10621 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10681 ccgaaaagtg ccacctgac
//
