LOCUS       sequence-458424-       10962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ABE activity-based selection cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10962)
  AUTHORS   Kaplan EG, Steger RJ, Shah ST, Drepanos LM, Griffith AL, Reint G, 
            Doench JG
  TITLE     Activity-based selection for enhanced base editor mutational 
            scanning.
  JOURNAL   Nat Genet. 2025 Nov;57(11):2920-2929. doi: 
            10.1038/s41588-025-02366-0. Epub 2025 Oct 14.
  PUBMED    41087678
REFERENCE   2  (bases 1 to 10962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-025-02366-0"; journalName: "Nat Genet"; date: 
            "2025-11"; volume: "57"; issue: "11"; pages: "2920-2929"
COMMENT     Sequence Label: pRDB_345
FEATURES             Location/Qualifiers
     source          1..10962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2615..2863
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2615..2635
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2786..2805
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        complement(3154..3368)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(3214..3233)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        3375..4553
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3606..4544
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4501..4521
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4558..4567
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4564..4566,4567..4569,4570..5280)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4564..4566,4567..4569,4570..5280)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4609..4630)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4870..4889)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5217..5238
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5287..5343
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     5934..5954
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6054..6642
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6107..6127)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6525..6536)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6714..6947
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6973..6990)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6979..7203
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7249..7677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7336..7355)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7546..7567
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7686..7706)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7691..8020
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7871..8006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7933..7952
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8068..8115
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8134..8508
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8638..8759
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8675..8694)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8729..8748
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8808..8824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8808..8824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8821..8843)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8832..8848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8856..8862,8863..8880,8881..8886))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8901..8922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9039..9056)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9210..9798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9290..9309)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9969..10760,10761..10829))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10592..10611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10830..10934)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccggg cagagagggc ctatttccca tgattccttc
     2641 atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa
     2701 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     2761 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     2821 cgatttcttg gctttatata tcttgtggaa aggacgaaac accggagacg cttttttcgt
     2881 ctcagtttca gagctaagca caagagtgca tagcaagttg aaataaggct agtccgttta
     2941 caacttgaaa aagtggcacc cgagtcgggt gcttttttga aagctccaca gtgcagggga
     3001 aagaatagta gagacccaat ggaaaaaagc accgactcgg tgccactttg ctgtttccag
     3061 caaagttgat aacggactag ccttatttaa acttgctatg ctgtttccag catagctctt
     3121 aaacccgatt accctacaca tctgcgggaa agagtggtct catacagaac ttataagatt
     3181 cccaaatcca aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa
     3241 atattgcagg gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc
     3301 gctgggtgtt cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt
     3361 cagcgttcga acacggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     3421 ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg
     3481 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     3541 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     3601 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
     3661 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
     3721 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
     3781 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     3841 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     3901 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
     3961 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     4021 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     4081 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     4141 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     4201 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     4261 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     4321 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     4381 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     4441 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     4501 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaaggagcc
     4561 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     4621 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     4681 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     4741 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     4801 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     4861 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     4921 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     4981 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     5041 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     5101 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5161 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     5221 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     5281 ggaagcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     5341 ccgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     5401 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5461 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5521 atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag
     5581 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5641 tcccggctgg cagatgtgta gggtaatcgg gggttttaat tcaggtcccg gatccggtgg
     5701 tggtgcaaat caaagaactg ctcctcagtg gatgttgcct ttacttctag cgcagcaaca
     5761 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5821 cggagtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5881 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5941 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6001 gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcc tgataaattc gaaaatcaac
     6061 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6121 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6181 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6241 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6301 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6361 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6421 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     6481 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6541 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cctcgccttc
     6601 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
     6661 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6721 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     6781 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6841 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6901 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     6961 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     7021 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     7081 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     7141 acagcaaggg ggaggattgg gaagataata gcaggcatgc tggggatgcg gtgggctcta
     7201 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     7261 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7321 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7381 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7441 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7501 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7561 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7621 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     7681 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     7741 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     7801 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     7861 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7921 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     7981 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     8041 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     8101 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     8161 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     8221 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     8281 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     8341 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     8401 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     8461 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     8521 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8581 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8641 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8701 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8761 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8821 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8881 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8941 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9001 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9061 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9121 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9181 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9241 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9301 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9361 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9421 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9481 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9541 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9601 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9661 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9721 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9781 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9841 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9901 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9961 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10021 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10081 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10141 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10201 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10261 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10321 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10381 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10441 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10501 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10561 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10621 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10681 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10741 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10801 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10861 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10921 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
