LOCUS       sequence-458439-       12111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector - mCherry reporter and huCD25 for Gal4DBDVP64 
            synNotch receptors with a constitutive BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12111)
  AUTHORS   Reddy NR, Maachi H, Xiao Y, Simic MS, Yu W, Tonai Y, Cabanillas DA, 
            Serrano-Wu E, Pauerstein PT, Tamaki W, Allen GM, Parent AV, Hebrok 
            M, Lim WA
  TITLE     Engineering synthetic suppressor T cells that execute locally 
            targeted immunoprotective programs.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4793. doi: 
            10.1126/science.adl4793. Epub 2024 Dec 6.
  PUBMED    39636990
REFERENCE   2  (bases 1 to 12111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4793. doi"; pages: " 
            10.1126/science.adl4793. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR-GAL4-UAS-huCD25- IRES-mCherry-PGK-BFP
FEATURES             Location/Qualifiers
     source          1..12111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(241..259)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(242..259)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(549..568)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(782..801)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     901..923
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(961..979)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1047..1151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1152..1220,1221..2012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1370..1389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2183..2771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2672..2691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2925..2942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3012..3032)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3017..3346
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3197..3332
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4480..4545
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4675..4695
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5120..5254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5157..5176)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5211..5230
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5422..6055
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6102..6227
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6724..6957
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7142..7186
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7471..7588
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     7814..7838
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      7918..7927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8764..9337
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8931..8948)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9158..9177
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9356..10066
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9501..9519)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9769..9788
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        10151..10638
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10186..10209)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10571..10590
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10659..11360
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    11396..11984
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11449..11469)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11867..11878)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11987..12003)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11988..12004)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             join(12094..12111,1..216)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
       61 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      121 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      181 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
      241 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
      301 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac
      361 cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt
      421 ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc
      481 tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      541 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      601 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      661 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
      721 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca
      781 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
      841 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      901 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
      961 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1021 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1081 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1141 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1201 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1261 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1321 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1381 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1441 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1501 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1561 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1621 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1681 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1741 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1801 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1861 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1921 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1981 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2881 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2941 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     3001 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3061 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3181 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3241 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3301 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     3361 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     3421 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     3481 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     3541 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     3601 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     3661 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     3721 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     3781 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     3841 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     3901 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     3961 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     4021 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     4081 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     4141 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     4201 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     4261 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     4321 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     4381 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     4441 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     4501 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     4561 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     4621 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     4681 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     4741 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     4801 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     4861 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     4921 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     4981 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     5041 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     5101 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     5161 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5221 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     5281 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     5341 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     5401 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     5461 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     5521 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     5581 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     5641 tgcatgggat ggatgacccg tagagagaag tgttagagtg gaggtttgac agccgcctag
     5701 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     5761 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     5821 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     5881 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5941 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6001 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     6061 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     6121 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     6181 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     6241 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     6301 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     6361 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     6421 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     6481 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     6541 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     6601 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     6661 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     6721 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     6781 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     6841 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     6901 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     6961 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     7021 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     7081 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     7141 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     7201 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     7261 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     7321 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     7381 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     7441 gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     7501 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     7561 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     7621 ttggatcgat aagcttgata tcgaattcgg agcactgtcc tccgaacgtc ggagcactgt
     7681 cctccgaacg tcggagcact gtcctccgaa cgtcggagca ctgtcctccg aacggagcat
     7741 gtcctccgaa cgtcggagca ctgtcctccg aacgactagt taggcgtgta cggtgggagg
     7801 cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     7861 gttttgacct ccatagaaga caccgggacc gatccagcct ctcgacattc gtggatcgcc
     7921 gccatggatt catacctgct gatgtgggga ctgctcacgt tcatcatggt gcctggctgc
     7981 caggcagagc tctgtgacga tgacccgcca gagatcccac acgccacatt caaagccatg
     8041 gcctacaagg aaggaaccat gttgaactgt gaatgcaaga gaggtttccg cagaataaaa
     8101 agcgggtcac tctatatgct ctgtacagga aactctagcc actcgtcctg ggacaaccaa
     8161 tgtcaatgca caagctctgc cactcggaac acaacgaaac aagtgacacc tcaacctgaa
     8221 gaacagaaag aaaggaaaac cacagaaatg caaagtccaa tgcagccagt ggaccaagcg
     8281 agccttccag gtcactgcag ggaacctcca ccatgggaaa atgaagccac agagagaatt
     8341 tatcatttcg tggtggggca gatggtttat tatcagtgcg tccagggata cagggctcta
     8401 cacagaggtc ctgctgagag cgtctgcaaa atgacccatg ggaagacaag gtggacccag
     8461 ccccagctca tatgcacagg tgaaatggag accagtcagt ttccaggtga agagaagcct
     8521 caggcaagcc ccgaaggccg tcctgagagt gagacttcct gcctcgtcac aacaacagat
     8581 tttcaaatac agacagaaat ggctgcaacc atggagacgt ccatatttac aacagagtac
     8641 caggtagcag tggccggctg tgttttcctg ctgatcagcg tcctcctcct gagtgggctc
     8701 acctggcagc ggagacagag gaagagtaga agaacaatct cctgagatcc ttgacttgcg
     8761 gcccccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     8821 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     8881 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     8941 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     9001 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     9061 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     9121 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     9181 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     9241 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     9301 cggggacgtg gttttccttt gaaaaacacg atgataaatg agcgctggcg ggtccatggt
     9361 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     9421 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     9481 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     9541 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     9601 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     9661 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     9721 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     9781 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     9841 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     9901 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     9961 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
    10021 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtagggcc gcaactccca
    10081 cctgcaacat gcgtgactga ctgaggccgc gactctagag tcgacctgca cgaggttaac
    10141 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
    10201 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
    10261 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
    10321 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
    10381 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
    10441 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
    10501 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
    10561 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
    10621 tcatctccgg gcctttcgac ctgcagccca agcttaccat gagcgagctg attaaggaga
    10681 acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat
    10741 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
    10801 gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga
    10861 ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct
    10921 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
    10981 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat
    11041 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt
    11101 accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga
    11161 gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca
    11221 agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg
    11281 agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac
    11341 tggggcacaa gcttaattaa tgcaggcatg caagcttgat atcaagctta tcgataatca
    11401 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
    11461 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
    11521 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
    11581 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
    11641 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
    11701 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
    11761 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
    11821 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
    11881 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
    11941 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
    12001 tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
    12061 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac t
//
