LOCUS       sequence-458484-       30615 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  Chromosomal gene manipulation (gene insertion, conversion, deletion)
            of dairy used Lactobacillus bulgaricus, a difficult gene to 
            manipulate, is now possible by conjugation using this pGMB1 
            plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 30615)
  AUTHORS   Iwamoto D, Ishizaki M, Miura T, Sasaki Y
  TITLE     Novel shuttle vector pGMbeta1 for conjugative chromosomal 
            manipulation of Lactobacillus delbrueckii subsp. bulgaricus.
  JOURNAL   Biosci Microbiota Food Health. 2022;41(1):20-29. doi: 
            10.12938/bmfh.2021-014. Epub 2021 Oct 18.
  PUBMED    35036250
REFERENCE   2  (bases 1 to 30615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Microbiota Food Health."; date: "2022"; volume: "41(1):20-29. doi"; 
            pages: " 10.12938/bmfh.2021-014. Epub 2021 Oct 18"
COMMENT     Sequence Label: pGM-beta-1
FEATURES             Location/Qualifiers
     source          1..30615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(7391..7409)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7392..7409)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7427..7443)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7427..7443)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7440..7462)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7451..7467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7475..7481,7482..7499,7500..7505))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7520..7541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7658..7675)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7829..8417)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7909..7928)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8588..9379,9380..9448))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9211..9230
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9449..9553)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9631..10086)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9768..9789)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9980..9999
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10212..10234
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10226..10243
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10227..10243
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10250..10269
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10250..10268
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      10299..10308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10303..10312
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             21414..21425
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 acatgcgctt agaatcgctt taggaaacac gatccagtcc aataatcgtc gataaaaact
       61 tttgaaaaag gttggtgaaa ttacctactt ttggaataat cacaaatcac aagtgattaa
      121 tcacaaatca caagtgatta atcacttgtt tattaagata ttaaaagcta taatttaaat
      181 aaagcgtgaa ttttattaca caaaaagagg ggggagaaac ttggaactag catttagaga
      241 aagcttaaaa aagatgagag gtaccaaatc aaaagaaaaa ttctcccaag aattagaaat
      301 gagtagatca aattattcac gaatagaatc aggaaaatca gatccaacca taaaaacact
      361 agaacaaatt gcaaagttaa ctaactcaac gctagtagtg gatttaatcc caaatgagcc
      421 aacagaacca gaaccagaaa cagaatcaga acaagtaaca ttggatttag aaatggaaga
      481 agaaaaaagc aatgacttcg tgtgaataat gcacgaaatc gttgcttatt ttttttaaaa
      541 gcggtatact agatataacg aaacaacgaa ctgaatagaa acgaaaaaag agccatgaca
      601 catttataaa atgtttgacg acattttata aatgcatagc ccgataagat tgccaaacca
      661 acgcttatca gttagtcaga tgaactcttc cctcgtaaga agttatttaa ttaactttgt
      721 ttgaagacgg tatataaccg tactatcatt atatagggaa atcagagagt tttcaagtat
      781 ctaagctact gaatttaaga attgttaagc aatcaatcgg aaatcgtttg attgcttttt
      841 ttgtattcat ttatagaagg tggagtttgt atgaatcatg atgaatgtaa aacttatata
      901 aaaaatagtt tattggagat aagaaaatta gcaaatatct atacactaga aacgtttaag
      961 aaagagttag aaaagagaaa tatctactta gaaacaaaat cagataagta tttttcttcg
     1021 gagggggaag attatatata taagttaata gaaaataaca aaataattta ttcgattagt
     1081 ggaaaaaaat tgacttataa aggaaaaaaa tctttttcaa aacatgcaat attgaaacag
     1141 ttgaatgaaa aagcaaacca agttaattaa acaacctatt ttataggatt tataggaaag
     1201 gagaacagct gaatgaatat cccttttgtt gtagaaactg tgcttcatga cggcttgtta
     1261 aagtacaaat ttaaaaatag taaaattcgc tcaatcacta ccaagccagg taaaagcaaa
     1321 ggggctattt ttgcgtatcg ctcaaaatca agcatgattg gcggtcgtgg tgttgttctg
     1381 acttccgagg aagcgattca agaaaatcaa gatacattta cacattggac acccaacgtt
     1441 tatcgttatg gaacgtatgc agacgaaaac cgttcataca cgaaaggaca ttctgaaaac
     1501 aatttaagac aaatcaatac cttctttatt gattttgata ttcacacggc aaaagaaact
     1561 atttcagcaa gcgatatttt aacaaccgct attgatttag gttttatgcc tactatgatt
     1621 atcaaatctg ataaaggtta tcaagcatat tttgttttag aaacgccagt ctatgtgact
     1681 tcaaaatcag aatttaaatc tgtcaaagca gccaaaataa tttcgcaaaa tatccgagaa
     1741 tattttggaa agtctttgcc agttgatcta acgtgtaatc attttggtat tgctcgcata
     1801 ccaagaacgg acaatgtaga attttttgat cctaattacc gttattcttt caaagaatgg
     1861 caagattggt ctttcaaaca aacagataat aagggcttta ctcgttcaag tctaacggtt
     1921 ttaagcggta cagaaggcaa aaaacaagta gatgaaccct ggtttaatct cttattgcac
     1981 gaaacgaaat tttcaggaga aaagggttta atagggcgta ataacgtcat gtttaccctc
     2041 tctttagcct actttagttc aggctattca atcgaaacgt gcgaatataa tatgtttgag
     2101 tttaataatc gattagatca acccttagaa gaaaaagaag taatcaaaat tgttagaagt
     2161 gcctattcag aaaactatca aggggctaat agggaataca ttaccattct ttgcaaagct
     2221 tgggtatcaa gtgatttaac cagtaaagat ttatttgtcc gtcaagggtg gtttaaattc
     2281 aagaaaaaaa gaagcgaacg tcaacgtgtt catttgtcag aatggaaaga agatttaatg
     2341 gcttatatta gcgaaaaaag cgatgtatac aagccttatt tagtgacgac caaaaaagag
     2401 attagagaag tgctaggcat tcctgaacgg acattagata aattgctgaa ggtactgaag
     2461 gcgaatcagg aaattttctt taagattaaa ccaggaagaa atggtggcat tcaacttgct
     2521 agtgttaaat cattgttgct atcgatcatt aaagtaaaaa aagaagaaaa agaaagctat
     2581 ataaaggcgc tgacaaattc ttttgactta gagcatacat tcattcaaga gactttaaac
     2641 aagctagcag aacgccctaa aacggacaca caactcgatt tgtttagcta tgatacaggc
     2701 tgaaaataaa acccgcacta tgccattaca tttatatcta tgatacgtgt ttgttttttc
     2761 tttgctgttt agcgaatgat tagcagaaat atacagagta agattttaat taattattag
     2821 ggggagaagg agagagtagc ccgaaaactt ttagttggct tggactgaac gaagtgaggg
     2881 aaaggctact aaaacgtcga ggggcagtga gagcgaagcg aacacttgat tttttaattt
     2941 tctatctttt ataggtcatt agagtatact tatttgtcct ataaactatt tagcagcata
     3001 atagatttat tgaataggtc atttaagttg agcatattag aggaggaaaa tcttggagaa
     3061 atatttgaag aacccgatta catggattgg attagttctt gtggttacgt ggtttttaac
     3121 taaaagtagt gaatttttga tttttggtgt gtgtgtcttg ttgttagtat ttgctagtca
     3181 aagtgattaa atagaattca tatccaattt atttttttct taacaaggga ggtgtttttt
     3241 aacatgacta aagtagggta tgcacgtgtc agtagcaaag aacagaactt agatagacaa
     3301 ctgaaagcgt tagagggcgt ttctaaggtc ttttcagaca aagcaagcgg tcaatcggtc
     3361 gaacgcccac aattacaagc tatgcttaac tatattcgtg aaggggatat agttgttgtt
     3421 actgaattag atcgattagg acgaaataat aaagaattaa cagaattgat gaatcaaatt
     3481 caaattaagg gggcaaccct ggaagtctta aatttaccct caatgaatgg tattgaagat
     3541 gaaaatttaa gacggctgat taataattta gtgattgaat tgtataagta ccaagcggaa
     3601 tctgaacgca aacgaattaa agaacgccaa gcccaaggaa ttgaaattgc taagaaaaaa
     3661 ggaaaattca aagggcgaca actgaaattc aaagaaaatg atccacgttt acaacacgct
     3721 ttcgatttgt ttttgaacgg tttatccgat aaagaagttg aagaacaaac tggaattaat
     3781 cgccgaacgt ttagaaggta tcgatcaaga tacaacgtga cagtcgatca aagaaaaaac
     3841 aatgaaaaga gggatagtta atgagtacgg ttattttagc tgaaaaacca agccaggcat
     3901 tagcctatgc aagtgcttta aaacaaagca ccaaaaaaga cggttatttt gagatcaaag
     3961 acccaatctt tgcagatgaa acgtttatca cgtttggttt tgggcattta gtcgagttag
     4021 cagaaccagg tcattatgac gaaaagtggc aaaattggaa acttgaatca ttgccgattt
     4081 ttcctgatcg atacgatttt gaagtggcaa cagataaaaa aaagcagttt aaaattgttg
     4141 ctgaactttt aaaacaagca aatacaatca ttgtcgcaac agatagcgac agagaaggcg
     4201 aaaacattgc ctggtcgatc attcataaag caaatgcctt ttctaaagat aaaacgtata
     4261 aaagactatg gatcaatagt ttagaaaaag atgtgatccg tagcggtttt caaaatttgc
     4321 aaccaggaat gaattactat cccttttatc aagaagcgca aacacgccaa attgccgatt
     4381 ggttgatcgg catgaatgca agccctttgt atacgttaaa tttacagcag aagggcgtac
     4441 aaggtacatt ttcactagga cgtgttcaaa cgcccacctt atatcttatt tttcagcgcc
     4501 aggaagccat agaaaacttt agaaaagaac cttttttcga ggtggaagct agtataaaag
     4561 taaaccaagg gtcatttaag ggcgttataa gccccacaca gcgctttaaa acccaagagg
     4621 agcttttagc ttttgtttct tctgaacaag ctaaaatagg caatcaagag gggataattg
     4681 ctgatgttca aaccaaagag aagaaaacga atagtccgag tttgttttct ttaagtagtt
     4741 tgcaatcaaa agttaatcag ctttataaag cgacagcgag ccaaacttta aaagctatgc
     4801 aaggactgta tgaagcaaaa ttattgagtt atccaagaac agatacacca tttattacag
     4861 agaacgaatt tgcttattta aaagcgaatt ttggcaaata tagcggtttt ttaggacttg
     4921 atcttgaaat ggttcaaaca gagcctagaa agcgttatgt ggacggtagt aaggtacagg
     4981 aacaccacgc cattatccca acaaaacaag tacctaccga atctgcatta gcgaaaatgg
     5041 acgatttaca acgaaaaata tatgctttag tcgttaaaac gaccgttgcc atgtttctac
     5101 ctgattattt gtatgaagaa actaagatac aaaccaaagt agccgactta ctttttcaat
     5161 caataggcaa gacaccaaag caagaaggtt ggaaaattct tttcaaacaa caaaccaaag
     5221 aagaagaaga ggacgttcaa acgttaccct tggttatcat tggagaacat gccgaggttg
     5281 acgttaagag tgccgaaaaa gaaacacaac caccgaaagc ttttacagag ggtacattat
     5341 taactgctat gaaaacggcg aataaaacgg ttgatgatga agaagcaatc aagattttac
     5401 aagaagttga ggggattgga acagaagcga caagagcaag cattattgaa gccttgaaac
     5461 aaaaagaata tatccaagtg attaagaata agcttgttgt aactgaaaaa ggaaaattat
     5521 tgtgccaggc agttgaaagt cagcaccttt taacgagtgc tgaaatgacg gctaaatggg
     5581 aaacgtattt aaaaaaaatc ggtaaaagag aaggcaatca agagaacttt attacgaata
     5641 tcaaaaaatt cattgttcat ttactggaag ctgtacctaa cgatatagaa aaactaaatt
     5701 tttctgatta ccaggaacag aaagaaaaag aagcagaaaa aagtattgta ggaaaatgtc
     5761 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
     5821 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actcaccaaa acaaatgtaa
     5881 aagaattact agagggaaaa gaaaccctgg taaaaggaat caaaacgaaa gatagaaagt
     5941 cctacaatgc cgttgtaaaa atcggagaaa agggatatat tgattttata tctttctcaa
     6001 aataaacata aaagcccttt aaagagggct tttatatatt aatcacaaat cacttatcac
     6061 aaatcacaag tgatttgtga ttgttgatga taaaataaga ataagaagaa atagaaagaa
     6121 gtgagtgatt gtgggaaatt taggcgcaca aaaagaaaaa cgaaatgata caccaatcag
     6181 tgcaaaaaaa gatataatgg gagataagac ggttcgtgtt cgtgctgact tgcaccatat
     6241 cataaaaatc gaaacagcaa agaatggcgg aaacgtaaaa gaagttatgg aaataagact
     6301 tagaagcaaa cttaagagtg tgttgatagt gcagtatctt aaaattttgt ataataggaa
     6361 ttgaagttaa attagatgct aaaaatttgt aattaagaag gagtgattac atgaacaaaa
     6421 atataaaata ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac
     6481 aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa
     6541 cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat
     6601 tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata
     6661 ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggagt attccttacc
     6721 atttaagcac acaaattatt aaaaaagtgg tttttgaaag ccatgcgtct gacatctatc
     6781 tgattgttga agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc
     6841 tcttgcacac tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc
     6901 ctaaaccaaa agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag
     6961 ataaatattg gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc
     7021 aactgtttac taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa
     7081 gtaccgttac ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga
     7141 ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt tacacgttac taaagggaat
     7201 gtagataaat tattaggtat actactgaca gcttccaagg agctaaagag gtccctagcg
     7261 cctacgggga atttgtatcg ataaggggta caaattccca ctaagcgctc gggcactagt
     7321 gaattcgcgg ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata
     7381 gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc
     7441 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7501 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7561 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7621 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7681 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7741 cagaatcagg ggataacgca gggaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7801 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcctccct gacgagcatc
     7861 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7921 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7981 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8041 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8101 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8161 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8221 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8281 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8341 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8401 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8461 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8521 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8581 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8641 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8701 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8761 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8821 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8881 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8941 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9001 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9061 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9121 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9181 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9241 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9301 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9361 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9421 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9481 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9541 taggggttcc gcgcacattt ccccgaaaag tgccacctga tgcggtgtga aataccgcac
     9601 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat
     9661 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     9721 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     9781 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     9841 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     9901 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     9961 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
    10021 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
    10081 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    10141 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    10201 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
    10261 actatagggc gaattgggcc cgacgtcgca tgctcccggc cgccatggcg gccgcgggaa
    10321 ttcctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg tattaaaaaa
    10381 atcgttttat ggttgttcaa attatcctga atgtaccttc actttagctg aacattttag
    10441 aaagaaaaaa ctcaccaaaa caaatgtaaa agaattacta gagggaaaag aaaccctggt
    10501 aaaaggaatc aaaacgaaag atagaaagtc ctacaatgcc gttgtaaaaa tcggagaaaa
    10561 gggatatatt gattttatat ctttctcaaa ataaacataa aagcccttta aagagggctt
    10621 ttatatatta atcacaaatc acttatcaca aatcacaagt gattaatcac aaatcacttg
    10681 tgatttgtga ttcctaatga tacaatatta ctatacaaaa aaagaatggg gcgtagttat
    10741 ggagaaggaa gaactcaaaa tacttgaaga attaagacgt attttaaaca gtaaaaatga
    10801 agcgattgtt atcttaaaca attattttaa aggtggtgtt ggaaagtcaa aattatcgac
    10861 tatgtttgct tacttgacag acaaatttaa tttaaaagtt ttaatgatcg ataaggactt
    10921 acaagcaaca ttgacaaaag acttagcaaa aacatttaag gtagaattgc cacgtgttaa
    10981 tttttatgaa ggactgaaaa atggaaactt ggcttcttct attgttcatt tgactgataa
    11041 tttagacttg atccctggca cgtttgattt gatgttactg ccaaaattaa ctcgctcatg
    11101 gacttttgaa aatgaaagta gattgcttgc tactctttta gcacctttaa aaagtgacta
    11161 tgatctcatt attattgata ctgtaccaac gccaagcgtt tatacaaata atgcaatcgt
    11221 ggcaagtgat tacgtcatga tccctttaca agcagaagaa gaaagtacaa acaacattca
    11281 aaactatatt tcctatttga ttgatttaca agaacagttt aaccctggac tagatatgat
    11341 cggttttgtt ccttatttag ttgatacgga cagcacaacg ataaaatcaa acctggaaga
    11401 actgtacaag caacataaag aagataactt ggttttccaa aatattatca agcgaagtaa
    11461 taaagtaagt acctggtcta aaaacggcat tacagaacac aaaggctatg acaaaaaagt
    11521 tttatctatg tatgagaacg tattttttga aatgcttgag cgaatcattc aattagaaaa
    11581 cgaaaaagaa tagaatcaca aatcacaagt gattaatcac aaatcacttg tgatttgtga
    11641 ttgttgatga taaaataaga gtaagaagaa atagaaagaa gtgagtggtt gtgggaaatt
    11701 taggcgcaca aaaagcaaaa cgaaatgata caccaatcag tgcaaaaaaa gatattatgg
    11761 gggataagac ggttcgtgtt cgtgctgact tgcaccatat tataaaaatc gaaacagcaa
    11821 agaatggcgg aaacgtaaaa gaagttatgg ataaagcctt agaagaatat attcggaaat
    11881 atttacctga caaactttaa aaggagtgaa aatgaaatgg cagttactta tgaaaaaaca
    11941 tttgaaatag agatcattaa cgaattatcg gcaagcgttt ataatcgagt attaaactat
    12001 gttttgaatc atgaattaaa taaaaatgac tctcaattat tggaagtcaa tttattaaac
    12061 caattaaagc ttgcaaaacg tgtaaatctt tttgattatt ctttagaaga attacaagcc
    12121 gttcatgagt attggcggtc aatgaatcgc tattcaaaac aagttttgaa taaagagaaa
    12181 gtggcttaat atggcaaata tagtcaattt tactgacaaa caatttgaaa atcgcttaaa
    12241 tgataattta gaagaattgg ttcaaggaaa aaaagcggtt gaatcgccaa ccgctttttt
    12301 acttggtggg caaccagggt cagggaaaac gagtttgcga tcagcaattc ttgaagaaac
    12361 acaagggaat gttattgtca ttgataatga tacctttaaa caacagcacc ctaattttga
    12421 tgaactagcg aaactttatg agaaagacgt agtaaaacac gttacccctt attctaatcg
    12481 catgacagaa gcgatcataa gccgtttaag cgatcaaggc tataacttgg tgattgaagg
    12541 tacaggacga acaacagacg ttcccattca aaccgcaaca atgcttcaag ccaaaggtta
    12601 tgaaacaaaa atgtatgtca tggcagtacc taaaatcaac tcatatttag gaacaattga
    12661 acgatatgaa accatgtatg cagatgatcc aatgacagcc agggcaacac caaaacaagc
    12721 gcatgatatt gttgtcaaaa acttaccgac caatttagaa acccttcata aaacgggctt
    12781 atttagcgat ataaggcttt acaatagaga aggagtgaaa ctctattcaa gcttagaaac
    12841 gccttccatt agtccgaaag agaccttaga aagagaatta aatcgtaaag tatcagggaa
    12901 agaaattcaa ccgactttgg agagaataga gcaaaaaatg gttcaaaatc aacaccaaga
    12961 aacccctgaa tttaaagcaa ttcaacaaaa attggaaagc ttgcagccac ctacaccacc
    13021 aatacccaaa acacctaaac ttccaggact ttaaacctta aaaaaggaaa agagtagtta
    13081 ccaaaaaacg gtaactactc tttttttata aaaacatttc ctcatgtttt aaaagctgat
    13141 cgagaaataa agcacacaac ggtattcttt cttttttcag ttggtacatt ttgcagtctg
    13201 taacgtgtca tttactgttt gttaccttaa catatccagt cgaaaacaca agggtatata
    13261 aacgagcaag ctcgcccttg tgttttcttt gtgtctatgt tgcgttctcg tcacagcaaa
    13321 cgacacgaac acactggcga aaatgaacca acaaaacgaa aaaagaaagg agcgtatact
    13381 gtaatttatt tctagctttt aaaacatgag gaaaacagct tcacttcctc aaacgcctaa
    13441 caaaaatggt tgtcaaaaaa ctaaaaatca aaaaaaagaa aagaggaaaa gaaaatggaa
    13501 aaaacaaaaa aattacaatt agaagatttt acagaaaatg agttttttgg aacacaagaa
    13561 cagaaatatc taaaagctca agtaagagaa gagctaaaag agcaaggctt tatcatagat
    13621 agcagttttg agggcgattt taaaacatgg ataggtgtat acgcaagacc aaaagataaa
    13681 ccgacctact tagaccctca aaacgataaa gaagcagaag aacaagagca atattctatt
    13741 aacggcttta aacaagattt tagcgaatgg tttgagtggg aaataaaaaa tttgaaaatt
    13801 aaagaaatgt aaaaaaaaag cctagcaaaa aaagctaggt ttttctttta ggtaaaacac
    13861 caaaaagaaa aacctaagaa ggataaaata agttgttcca cttgcggtgc agttgctcct
    13921 cgttccaaac ccaacttatt tctttgcaaa gatatttgta tttgactaac acctttcctc
    13981 gaatggtaaa agctaaccgt tcgttactct acctcagata ctttcttttt tcagttggta
    14041 cattttgcag tctgtaacgt gtcacttact gtttgttacc ttaacatatc cagtcgaaaa
    14101 cacaagggta tataaacgag caagctcgcc cttgtgtttt ctttgtgtct atgttgcgtt
    14161 ctcgccacag caaacgaaac gaacacactg gcgaaaatga accacctaaa cgaaaaaaga
    14221 aaggagcaag atatgtagaa cattcacagc ttttaccatt cgaggaaaac agcttcactt
    14281 cctcaaacgc ctaacaaaaa tggttgtcaa aaagccaaaa ataaaaaaga aaaggaatga
    14341 taaaaatgaa atacattaaa aacgttgaaa cattggaaga attgaaaaaa gcctacaaaa
    14401 agttagcttt gaagttacac cccgattgtg gcgggaatga agaagaaatg aaaattttga
    14461 acaatgaata tgacgaactg tttagcaaac ttaaaaacac tcataagaat aaagaaggtg
    14521 aaacttacac caaagaaaca acagaaacac ccgaacagtt taaggatatt attaataaat
    14581 tgttcaacct aaaaatggac ggcgtagcaa tcgaagtagt aggaacattt atatggctaa
    14641 caggtaatac aaaaccttat aaggacgaca taaaagccct agaatttcgt tattcaccaa
    14701 aaaaatacgc atggtataaa gcaccaagcg attataaaaa acgtagtcga aaaaattatg
    14761 atatggacac aataagggga atgtatggaa gtcaaaaggt gaaggaagat aaagaagaaa
    14821 aaaaatactt acaagccaat taaggcttgt aagtttatca gaaaggaagt tttaaagtgg
    14881 atattaaaat aaaaaaaata aattttgaag gtaatatttt aaaagttata aaagcgatag
    14941 taacagaaat gagaggaata aataatcatc aaaaatatga ttttgattta tatcaaatag
    15001 aagcacgttc gccaatgtca acaagagaaa taactttaac agttgatttt atagaaaaaa
    15061 aagtatcagg tgatattatc gcttttggtg attggtacga tttggatata gaatcagtaa
    15121 atgaaatatt aaagcaatta aaaaaggaag aacaaacatt aagaacaatc aattttattt
    15181 aaaaaagtat caaaaaaagg aaaattattt ttcctttttt tgatactttt taggtttatt
    15241 tgctttaata tattcagaaa aaatttttaa aaattcttcc gtttcttcta cagttaaata
    15301 gtcaatttca aaatactttt ctaataaagc acctttttca attagacgct ttgttcttct
    15361 ttttctgtta atattttgct cgctattcgc tcgcaaaata tttttttgaa tttttagttt
    15421 ttctattctt ttgttgatgt catttaaatc attatttacc ataactattt aagtaattca
    15481 gaaaaatttt gactattcgc atttaataaa gtttctaatt cgtcatagaa taattctttg
    15541 ttttcttcaa tcgattcaat agaaatatca ttttttgaaa gagtagaaag gacaaaatca
    15601 ccaatatctg cttttgattt tagaattaat tcttctttct tcttttgtaa ttctttaatt
    15661 tgaagttcaa tatcagaaac agtttttttc tttctctttt ttggttttgt ttttggaatg
    15721 atttgttcat tttctgtctt gatgttttga tcttggtttt ccataaaata cccctcctaa
    15781 taatttatat atgtctatca tattatatta tttttctaaa atcaataaat ataaatggaa
    15841 atgtcaggat aaacatattt acttttataa tgataagtgg taaaattaat ttattaagga
    15901 ttcccttaga cgatttacta agggcgcact tataccccta aatttcattt agtggtaatt
    15961 gtgctataaa acttaattta catttcgttt tatcgtcaag cgtgtttgtt aaaattcgct
    16021 acgctcatgt ttgaaaagaa agagaggtga tacaattggc aatcttccat ttatctatga
    16081 caatagcaaa aagagaaggc ggaaaaagaa gtttaattgc aatggcttct tatcgaagcg
    16141 gtgaaaaatt gtatagtgaa ctatatgaaa aaactaatct atacaaccat agaactgtta
    16201 aaccagaagc ttttatttta aagcctgatt atgtacctaa tgaattttta gatagacaga
    16261 ctttatggaa caaaatggaa ttagcagaaa aacaggttaa ttctcaaatt tgccgagaag
    16321 taaatgtagc attgccaatt gaattaaata atacagatca acgatcactg atagaagaat
    16381 ttgttaaaga taattttgtc agcgaaggaa tgattgcaga cgtagccatt catagagatg
    16441 atgaaaacaa tccccatgct catattatgc taacaatgag agaagtcgat agtgaaggaa
    16501 atattcttaa caagcgaaaa aagataccta aactagatga aaatgggaac ccgattttat
    16561 tagaaaatgg taaaattaaa atggtttcta ttaaaacaaa tgattgggat agaaaatctc
    16621 ttgtttctga aattcgtaaa gattgggcag acaaagtcaa ccaatattta aaagatagaa
    16681 atatcgatca acaaattaca gaaaaatcgc atgcggaact tggaaaaaaa gaactaccaa
    16741 caattcatga aggtttttac tcaaaaaaat tagaagaaaa aggaattata agtgagttaa
    16801 aaagaaaaaa tttagagatt caaagttaca atgatattct agccgaactt gataaacttg
    16861 aaagtcaaga aaaagtctta aaacaagacc aaaacttcac tttaaaattt gaaaaaactt
    16921 tctcaccttt agaaaaagga gaactgaaaa atctttcaaa agaattgaaa ttatttatta
    16981 gtgatgaaaa cattgataaa cgattaggtg aattaaaacg atgggaaaat tcacttatct
    17041 ttaataataa aatggaaatt caaaaacaac gtttgatgtt aagtaaaatt agtagtgaac
    17101 gagatatgct tacaaaggca aatgaaattt tagataaaca agcagaaaga ttcttcaaaa
    17161 aatcttatcc aagtttgaat attgacaaat tttcaaatca cgaagttaga gcaatggtta
    17221 atgaaaccat atttagaaaa cagttattga ataaagacca gttagcagag gtcatttaca
    17281 atgaaagagt aatagaaaaa gaagaaagta aaaagatttt taaagaaaaa ccatttcaaa
    17341 ctagccgtta tcttgattca aaaattaaac aagttgaaga tagtataaca aaagaaaata
    17401 atcctgaaag aaaagaaatt ttatcaatta aaaaagaaaa actaatagga ataaaacaag
    17461 gattgataga atatgttcaa tcagaagttg aacggaaatt tgataaaaat gtttcaatag
    17521 attcagtcat agaaggcgaa atgttacttg caaaagctga ctattacaaa acaactgatt
    17581 tttctaaagt cgaaggagtt gctagattca gcagtgagga agttaattcc atgttggaac
    17641 aatcaaaagg cttcttaact aacattcaga cggtgaaaat tcctaatgat tgccagggcg
    17701 tattttttgt tcaagatagc atgaaacata ttgatgaact aagtccatta gcaaaacaca
    17761 atctgaaaaa ggtcgttaat cgcaatgctt atttacctga ttctgataag atagaattaa
    17821 gtaaagaaat tgaaaatacc aataaagatc aatcccaaga attggataaa gacgtaccag
    17881 aaaaaaatga agtgactgta aaaatgttcc aatttgcgaa gtcaattaat cgtttgttga
    17941 gtggtaacca actacagaaa aaacgaaacc tagacaaatt gattaagcaa acaaaagcaa
    18001 aaaaaaatca atcattacaa aggaatattc ctttgcgata aaataaaaac aagaggtgta
    18061 taaaatgaaa aaatttatca aagatacaaa gtttaagctt ggaagtgcgg ttgttgcgtt
    18121 gggtacattg tttattactg atccagtgtt tgcagccact gatccacaag caaaattagt
    18181 tcaagcaggt aacactataa aaggtgtttt aacagcctta attgttgtag ttggtgggat
    18241 tgcttgtgca aaaattgtta ttaaatattt gccgtctatt gatgatccac aagaaaaaaa
    18301 taccatgtat aaagccttgg gaacagcctt gcttgttacg gcattaggtg gggcgttggt
    18361 ttggttagta ccttgggcgt atggcttact tgcttaatag agaagggagt tagttatgaa
    18421 tagcgatcaa gtgaaacaag ccctattaga tttgttaaat gcagacactg aaaaagggcg
    18481 gacttggttt tttccgtcca atgtatctga tcggtacaca gtcattttag ggctagattt
    18541 aaaacaatca gcaaaagcga tcggtacggc attaataagc gtgttattgg caattcttat
    18601 tttccgtagc acagccgttt ttcctttaat tatctatgtc attgttggtt tggtgtcatt
    18661 tggtggtgta tgggcgtttt atacgattaa accaattaca gaccgcccta acatttctat
    18721 atctgatttt atgaagcaaa gaaaagactt ttctaaaaga ccaaaagtct attataaaaa
    18781 gccgaaagaa cgagtgtaaa agagaggtga tttcattgtt tgattttcta aaaacaagtt
    18841 ccaaaagtaa cgataataaa aaaagcgatc ctataaaaga aatgatttat tgggaagata
    18901 attcaagatt tcaaggcgta tttaaagatt tttttgtcgt ttatcagcca gaaaaaaagc
    18961 aatttagcct tgtgagcatg ctaaaagttg acggcttaaa cgttgatacc ttgccagtat
    19021 cagagcaaga agggttaaac gaagattttg gtgtctttct atctcaaaac gttctatatg
    19081 aaccgcagat cacttctaag aatgtaccag tagaaattga cgattttgta gaagcctggg
    19141 ggaatacagt agaaaattat cgcaaaatgc cagggcataa tgaagcttta ttacaactaa
    19201 aggctagtta ctattatcat tatagaaatt tagcaagtaa catggaaact tcaaagaaac
    19261 aacattttgt aattaattct gaaccaattt caaaggaaac ttacgacagt ttggaattgt
    19321 cctatcaagt gttacgcgat aagacaagaa caatcagaac agctttaatt gcatttttaa
    19381 gtaaatatga ttgtcaggtt gaaatgtgta cgattggcga gatgaaaaag gttttgaata
    19441 gttaggagcg ttaaaatgga gaagatacca aaagagaaaa ttattgtaat acccgaagtt
    19501 gatacggacg ttgtatctga tttagcacca tttaacttta cagtagaacg tgacaaatta
    19561 ttaatcgatg attcatacgc agttccctat gtcattacaa aatacaataa taaaccaaga
    19621 ggaaattggt ttaatcgtat tcgtaaaatg agtggagata taaccatatc tcattactac
    19681 actaaagcaa acggtaactc attgaatgat tattacaaca gaaccattaa gaacaagcaa
    19741 gcagagatcg atcgttcgca tgatccgttg acgattatcc gtttagaacg tgaaatgaaa
    19801 attgcacaaa cgcagttaga acaagccgtt gatgaaaaca cttcttatct ttacttgtat
    19861 acctatgttt tgattaaaag caagtcagaa gataagttga aaaaattgtg tgaagatttt
    19921 gaaacacgtt gtatcgcaag tggagtaaaa gccttgattc catatactat gattgataaa
    19981 gcgtattgga gtgccttgcc tttacaatct aatgaagtgc ctgaatacac gtacacaatc
    20041 gcaaactcaa tcagtgcaag tagtattttt ccttttgatg ataatgagtt aagcgtattt
    20101 actaaaaata tgattattga gggaattaat aaagatactg aaaatatcgt tagtattgat
    20161 tacaccaaca gaaaattagt agtcaatcgt aataaattcg tttttggttt atctggtggg
    20221 ggaaagacca cttacctaac gtcagactat ttaaaaaaat atgctttttc tgataattca
    20281 acagaactaa ggcacagaat cgttttattt gatcccgaag atgaacaaac agagcgtgta
    20341 cgttctctag ggggcgaaat aatcaatcta tcgtctatgt cagacgtccg tatcaatcca
    20401 tttcaaatct actcacgcaa tacgctagat gtcgatttaa aagaatcgtt atccgatttt
    20461 gaagaggacg aacttgtaga aaatcttgaa ataaagcata aagattatga aatgactgac
    20521 aatgatattg ataaagaaat cagtaaacga atgaatattt taacgcctta tttcctaatg
    20581 gtagatcatt ctttgactga tagtcaatta tccattatta aaatagaagc gaaaaaatgc
    20641 tataccactt tatacgagaa gaaaaacttg tcaaaaatgg aaaataccga ttttccaaca
    20701 ttttcagact tagaaaatcg attgaaagcc ttagaagaaa ctgatccaaa aagatacaaa
    20761 cgaattgaag attttattta ttcattagaa gattttacaa tcggaagtcg taccattttt
    20821 aacggtcata caaatataga cttaaacaat ccgttaattt gcttttcttt gcgagattta
    20881 cagaccgaag aagggatcag agatttagca tacctcaaca gctttagtta tctatttgaa
    20941 gaaataacca acaatccgca aattgtaacg tctgtttatg cagatgaatt tcacttttta
    21001 ttgaagaata aaattagtgc tgactttttc ttccaagcat ataaacgctt tagaaaatac
    21061 aatgctgatt gtaccgtatc aacccaacag attgatgatg tattaaaagc acctgataat
    21121 atcggtaaag caattattgg gaatagcttt acaaaagtat tctttggact tgatgaaacg
    21181 gaagcacaag gtatttcaaa tgagttgaaa cttaaactca caaaaaaaga attatcgttc
    21241 attacctcaa aacgtcaagg ggaagctttg ctttttcatg gtacaaagcg agcaaagata
    21301 aaagtagatt taacacagga agaaatgcgt ttgcttaacc caggcgaata tgaagatatt
    21361 tacggcgtta gtccgaagaa agagatcaac tggttgttaa gatcgaaaat tcaatagaag
    21421 ggagaaaata atgaaataca aaatcttgaa aaatttgcaa ttttactatc aagagaatgt
    21481 cattgtcgtt caaataaacg aaaaatattt gacgaatcga gaacatattt ttgatataga
    21541 agaaagtgaa caatattttg ttgatgtcga ggaaatttta accaaggacg gaaaactaga
    21601 aattgtttat caccgtccta atggctatac accactactt gaattgaaag aatatgctga
    21661 tttttataag ttggatatag tgaatcgatt acttgaaatg aatgtactag aaaaaacaaa
    21721 cacctatcta gcaatgcaaa atatcctact caaagataca cgtgacttgc tttttattta
    21781 taaagcagat cattttggta atttgcctta ctcaactaaa gaagaattag agcaatggaa
    21841 aaattttatt tgtagttttt ttggtaaata cacgcttgaa aagtatgaga agaaccgtat
    21901 cgaagttcta gcaaaagaaa aaaattcatt tttaaatgat atagaagcag ttgaaagcct
    21961 agaatcatta agagatttaa taaaaaatcg actaaccgaa gaacaaaaga atttcttttc
    22021 tactgaatta caggataaga aagcagacgt tcgaaaaatt cgcagaaata aaagcttaaa
    22081 aattgcgtta gttgtaggtg ttattgcgtt atatggtggt acggtttttc ttatgaaagt
    22141 aaatgagaat aaacaagtta cggctacaca gcaaagcgca gaaacagaga tcactatttt
    22201 aaataagatt attgataatg atagtgagaa tatcgaagag gatatgcaaa agctcaatta
    22261 tcctaagaaa aaacaagttg atatttacgt gaaacttggt gattatacca aggcttatga
    22321 acttgataaa aagtcagata aaaaaattat tcaaagtctg tacaaacaag gagaaaccga
    22381 aaaaatagaa gcccttgatt taccaggaag cgactattta gcagacttca aaaagatttt
    22441 agcgtatgac aattcaacag atattgagta tctggttcaa actagtaccg atacaacaat
    22501 tgttgaagct ttaatcgata aatcagtaaa agaaaaagac attccaacag tgaaaaatat
    22561 tcgtcaagta tcaattacac aaaagaaatt agcaatcgat cctaaacgtc aaatcagtat
    22621 gattgactta ttgattgaaa ataacagtga agaattagaa aatatgtata aggataattc
    22681 tttaaatgag gacttgaaga aaaaacaaac caatgacttg ttagaagaaa acaacacatt
    22741 gcttagtgaa aagattgaat taacaaacgc tgaaaaagat taggaaggtt ggtgatttca
    22801 tatcatggga gcaacagctg ctaaactgct tattcgttat cgaaaaccga tagcgaaagc
    22861 aattttaggc tttttcgtct ttatcattgt aatcttcctt cttattttta ataacgatca
    22921 aaccgcccct agtggcggac tagcaacaga aaacaaaaat ttaagtgaat ctgttttgag
    22981 gtacaaaagt accgttgaaa agtacgctag acaatatgac ggtatggaat atgtgcctta
    23041 tatcttagcc cttatgcaaa ttgaatcagg cggtgaaggt ggcgacccta tgcaatcaag
    23101 cgaaagttta ggactgcccc cgaacactat taaagacccc gaacgaagca tacaacgagg
    23161 agtagagttt tttgtagaaa atatgaaaag cgctataaac cgaggatcag atataaaaac
    23221 agctcttcaa gcgtataatt atggcggtgg ttttgttgat tttgtagtga agaatggcgg
    23281 aacgtattct tttgagttgg ctaaacaatt ttctattaat atgtcaggtg gcgaaaaggt
    23341 accttatgta aatgctttat ctactacaat gggctacaat taccgctata actacggaaa
    23401 tatgttctat gtgccaaaat tacttgaata cgttaacgaa agccaggcaa gcgcagaggg
    23461 cgttcaaaac aattctaata atactggcgc tgttcaagtt gatgtaccta gtgagtacaa
    23521 aaataaatta cgttatccaa gatatgatgg acacaactat aataccagtg gcagttatcc
    23581 ttttgggcaa tgtacttggt acgcttttaa tcgtatggct caaataggaa aaccagttga
    23641 tgattttatg ggcaatggtg gcgaatgggg ctataaaggg aaagctttag gctataaagt
    23701 aacaaataag cccaaagtag gtacggcaat ttctttccca cctggttctt ttggctctga
    23761 tccagtttac ggacatgttg cctttgtgga agtcgtaaat gcagacggta cgcttttagt
    23821 atcagaatgt aatgttgtaa acccaggaag tggaacaatc agttatcgag tagtgccgac
    23881 ttctattgca aacattgcaa gttatgttga ataaggagag gaagaaatgg agtttgacaa
    23941 aataaaagtt agtttgttta tctcaacgct tgttttactt gcggttacca taggcatgat
    24001 tgtttttgga ataaagcaag gcgaacagat ccgtcactta aaaagtgatt taaaaagcgt
    24061 acagacggaa ctagcagaca aacaagagga acaaaatact aaccaaagcg aatcggaaaa
    24121 aattatcaaa gaattttaca agaccgttta taactacgaa aaaagccaaa aagaaatatc
    24181 tatgacaacg gttaaagaat tagcaacaga caacgtttat caagaattac aaaacgagat
    24241 caacgtaaac aattcatata gcccacaaca aaacacgatt caaaagtctt cagtaaacga
    24301 aaatgaaata aaaattcttg cttatgaaag caaagataat acacaacagt atttagtaac
    24361 agcaccaatc caccaagttt ttaacggtac aaaaaatgat tttgaaataa accaaattat
    24421 ccaaattaaa aaccaacaaa ttacccaaag aacgaccatt caattaggag aggagtagaa
    24481 gcatggcgaa gaagaagcaa gagcaagtga aactgattgt cctagttata ctctatctac
    24541 tattattacc agtaacattg attgtattta aattattacc ttttatcatt gcgaatgata
    24601 caaatattga attgagtacc tttgttacaa ctattttaaa gcacccgatc aatacttttt
    24661 tagagatcat tcaatctact tacatgatcc cgttcttcat tgtacaaatt attgttttaa
    24721 tcgttttatt tgtctttcta caccctacca aacgccaacc gtttgaggtt gtcggaaaaa
    24781 caaaccctgt tcatggctcg gcttattggg gggaggaatc agaaataaat gcaccaaaaa
    24841 atgttcattt gatccctgaa aaatcaatga aaagcatatt agaaaagtca atgaggaggg
    24901 ggaaaaatga ctagtttgtt agcagaatca gacggtttaa tattggggaa atttccaagt
    24961 ggaaaagttg tgatccaacc cgaagatagc aaaataagca atcgaaatat ttttgtcgta
    25021 ggtggtccag gatcatttaa aacacaaagt tatgttttgc ctaatgttgt aaataatcgc
    25081 agtacgtcaa ttgttgtaac cgatccaaaa ggggaaattt acgagttaac caatgaaata
    25141 aaaaaagccc agggcttcaa aacggttgtc ataaatttta aagatttttt actaagttct
    25201 aggtacaatc cattacttta cattcgcaaa tcaaatgata caaacaagat tgcgaacgtc
    25261 atagtatcag caaagaatga ccccaaacgg aaagattttt ggttcaatgc acaattaaac
    25321 ttattaaata cgttgattaa atatgtttat tttgaatatg aaccaagcgc acgaacaatt
    25381 gagggcattt tagacttttt agaagaattt gatccacgct ataacgaaga aggtgtatca
    25441 gaacttgatg aacaatttga gcggttgcca gacggacacg aagcaaaacg cagttactat
    25501 ttaggcttcc gacaagcaca aagtgaagca agaccaaaca ttgttattag tctattgacg
    25561 actttacaag attttgttga taaagaagta tcagaattta ccagtacaaa cgattttttc
    25621 tttgaggaat taggaacgtc aaaaatttgt ttatacgtgc taatttcccc attagatcga
    25681 acttgggacg gcttagtgaa tctctttttc caacaaatgt ttacagagtt atatttttta
    25741 ggagataagc acaacgcaaa attgccagta cctttagtta tgttactaga tgaatttgtc
    25801 aatttgggct actttccaac atacgagaat tttcttgcaa cgtgtcgagg gtatcgtatt
    25861 tcagttagta ccatattgca atctttgccc cagggctttg aactttacgg agataagaaa
    25921 tttaaagcga tcattgggaa tcatgccatt aaaatatgtc taggtggtgt agaggaaacg
    25981 actgccgaat acttttcaag acaagtaaat gatacaacaa tcaaagtata tacaggcgga
    26041 acaagtgaaa gtaaaacgtc cgcaaaaaca actaaccgaa gcggaagtaa gtcagaaagc
    26101 tacggttacc aaaaaaggcg attgattacc gaaggggaag ttatcaattt acaacaagaa
    26161 gaaaatggca gaaaaagtat tgtgttaatt gacggtaaac cttacatgtt aagaaaaacg
    26221 ccacaatttg aattgttcgg aaatcttctt aaaaagcatg aaatttcaca gcaagattat
    26281 ataagcagtc aaacggaatt tgccaaagaa tacattgagg aattggaaaa acagcacaaa
    26341 caacgaaaag tacaactagc aagcgcccca atttttcacg agaagaaaca agaagatgat
    26401 ttgaataaaa aagaaataaa tctacctgaa caaccgatcg aatcagaagt aacagaagaa
    26461 gaggaagaaa aagaactatt aatgagtgat attctaagta gttttgatga aggtatagag
    26521 gaaaacacaa ccaacaatga caacagcgaa ttaccatttt aaagatcgga ggaaatcaaa
    26581 tgaatggatt gaaagaattt tttgggaata tcccaatgtt tcaaaagcaa gtagaacgag
    26641 aaatcgaaga aaaagggatt gaacctagtg agggtattaa aaaaatggaa tttagccgtc
    26701 aagtgatccg taacttagtg attggcgttt tagttgtcgg tattcttgct tttgtgggca
    26761 tttcttattt taacaataag aatcaaccac cagtgacacc aacagccacc accgcaagca
    26821 aagaaagtaa ccagtcagaa acaagcggag aagccacaga aaacagctca caagccgttc
    26881 aagggtcaag cgaccattta ctcaaattaa gtgcgaaaga acgagcagac gaagcgacag
    26941 aagcgtttga gagttggtac aagtctttct ctaatggtga cgtgatttta gaaatcaaca
    27001 aagaattatt gaaagaagga tcaggcggta caagcccgat tgaattacaa acaaaactga
    27061 ttgataactt aaaagccaaa tttggcgata aggtagcaga tgatttttat acgagtttgc
    27121 aaacaagttt caatttcaat ccagtgatcg ttgacggtag caaagggcta acgatttcta
    27181 agcaaaatga cgataaaagc caatggttgc gtacctggtt cttagatacc caaaaaacag
    27241 aaaagaatac gaaaattgtt ttgcgtaatg attttcccta tgagtgggca gattggcgta
    27301 acaaaggaca acatgatgaa aaagtaggat caatgttttc acaaattgat tgggataatg
    27361 atttgagata tgaggttata gggctagtaa ctagcaaaca agagaagaat attgaaaaca
    27421 ttggtggagt atttgtagtt atgcaataca acgaaaaaat aggaaaatgg caagttagtg
    27481 gaacgattgg tggtgtaatg taatatggac gtaatagacc aaataaaaga tacaggatcg
    27541 gatattttaa gttctgctgg tgaggctatc tctaatgtct ttgagggcgt aaaagagagt
    27601 gtggatagtg tattgcaccc tattgatacc ttgttaggga attggggcag tgacctttta
    27661 gaagccagta tgaaaattgc caataagacg acttttgata ttcccaatat cgaattaatt
    27721 agtaccatta ccaatgtgtt tgtctttgca acgacaacct ttgcgatttg tattgtgttg
    27781 tataaggtca ttgaagcgca aatacaagct tctaatggga gtggtgacgc attgaccgct
    27841 aatattgttc aaaagctgtt ttactcgtct attgccttag caacgttgcc ctgggcaatg
    27901 aactttttta tcaaaaagat tgttgcaccg atcggggaat atgtgattca acaagtcgct
    27961 aatgatttga acgtggaaac gatcggcgaa cgtttgcgta atttcttagt ttccaatttc
    28021 tttaatggtg gcgttagtgc cgttgttctc tttgtttttg tgatcttctt tgccttttcc
    28081 attgccaagt attttgtagc gatctgtgtc ttttatgccg atttcttggt actgactatg
    28141 ttaacaccat tagttgcctt atcgctttta acagacgaac aaaattactt tcaaatatgg
    28201 atcaaagaac tgttatccga agcactcaca atgcttatta agcttatttt gtacttactc
    28261 atgattacac tcatgattgc tgaaaaatac acgttaccaa actttatgtt aatggttggt
    28321 tgtggcttgt tgattatcaa aacaccgtca gccctacaaa atatgtggta ttcaaccaaa
    28381 gtgtcaaaag gtggcggact tggaaactta gcaatgatgg ctatgtttag aaaataagag
    28441 agggggataa cgattgtctt attattttga aatacgaatt attcttcccg aagaagaaaa
    28501 acaatttttg aatcgaaaat taagtaaaag cgaattatcc gaagtcacac actatttaca
    28561 acaaaagaca agtcgaggaa ttccagttaa atttcgtgta ggtattttta gagtagaaga
    28621 tcaaacaaaa attatgtctg ttacgctgaa tacaaaaaat accaaagaaa cggacgtaat
    28681 taacttgtta ttaaatcgag taacagatca acacgtttta gtttacttga atgaaccaac
    28741 agaaccaaca acgcttaata cgcaagaatt aaatcgtcag gagttaaaaa cgagtaatga
    28801 acgccaagaa ataccgcaga cagaaattcc aactgaaaca gtaaatgaac ctagtgttat
    28861 taaggaaata tcgaaaaaga accaagcaaa acaaacgaat agtagaaaag aatcactatc
    28921 agaatccata accaagaaaa atgttcccag aattcatttg ttcatatcta ttttgactct
    28981 ttttattgta ttattgatag gaataagtgt tattcaacaa gtacaacttc aatcagtcaa
    29041 aaaagagagc gaattattgg aagaacaaat tgaacgtgtc aaagaaacgg atatttctca
    29101 atcgaaaata gatacttttg gaagatactt tttgacttac tatttttcac aggaaaaaaa
    29161 tcaagaaaat tatcagtcaa gtttaagaac atatgtatcc gaaaaggtag atatatccga
    29221 ttggaaagcg ttagggaaaa cgcttaagag tgtcaattac tatggtagtg aacaaacaaa
    29281 aaaagggtat agcgttgaat accttctaaa tgtttcagta gacaatcgta gtaaaatgca
    29341 aaaaatcact tttgaggttg aaccaactaa aaacggcttt cttgtgacaa ctcaaccaaa
    29401 acttacagac ttctctttta actgaaatac gctaaaaaga gagtgcttaa acaatgggaa
    29461 aaattaattt aaatcaaatt tacacagcaa aagaaatgag tgaaaggata ggtaagaata
    29521 gaaattacct atcccaagct tatcgtaaca acaaacatga aatacttaaa aattttaatt
    29581 atcgaaaaat tggtggaaca attattttct ctgataatcc caataatgat ttatcgcaat
    29641 tgattacagc aaaagaagca agccagttgt taggaaagaa tgatgaatac tttgcccaca
    29701 tttataaacg gtttcctcat agactagagg gaatcgatca catttataca ggtaaaacat
    29761 tattcttaac gaaagaatcg ctagaagttt ttaaaaaaaa gatgaacaaa aatgtcagat
    29821 aaagcatatt tatgatatac tatttatgta cacaaatatt aatttttaag gagagaagaa
    29881 aatgaaaaaa attgcccttt taacactttt tagtacagcg ctattggtat cagccccact
    29941 tgttagtttt gcggacgaag caacaggtag ttcaggtgtc aatgttctag ctgatgatgt
    30001 agttgatcca gtagaaccaa ccgatccaac tactccggtt gatccagttg atccagttga
    30061 tccagttgat ccagttgatc cagttgatcc agttgatcca gtcgatccaa cagagccaac
    30121 ggaaccaaca gagccaacag agccaacaga gccaacggaa ccaacggagc caacggaacc
    30181 aacggaacca agcaagccga cagagccaac agaaccaagt aaaccagtag acacaaaccc
    30241 aatcgaaaac ccagttaata cagatacagg cgttgtgatt gttgcagttg aagatagtaa
    30301 accaatcatt caattagcag acggaacaac aaaaaaagtt gaagctaaag aaattggcgc
    30361 agacgttcaa aaagacggaa cagtaacggt taaaggttca gacggaaaaa tgaaagtttt
    30421 accaaaaaca ggagaaacag aaaatattgc attgtctgtc ctaggttcat taatggtatt
    30481 aggatcagca ttcattttta aaaagagaat ctaattaagt atttttaaaa gcatggtcgc
    30541 aagtttcact agcagccatg ctttttttga atctttgtag gacgttttaa agagaattac
    30601 ccactttaaa attaa
//
