LOCUS       sequence-458491-        8254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic construct encoding mNG in combination with mScarlet-dPCP
            via an EMCV-IRES sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Kuffner CJ, Marzilli AM, Ngo JT
  TITLE     RNA-stabilized coat proteins for sensitive and simultaneous imaging 
            of distinct single mRNAs in live cells.
  JOURNAL   Nat Methods. 2025 Sep 22. doi: 10.1038/s41592-025-02782-4.
  PUBMED    40983766
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2025 Sep 22. doi: 10.1038/s41592-025-02782-4."
COMMENT     Sequence Label: pcDNA3-mNG-IRES-mScarlet-dPCP
FEATURES             Location/Qualifiers
     source          1..8254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        31..410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        411..614
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     565..585
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     659..678
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        659..677
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      691..700
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             697..1407
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      952..961
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1543..2092
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1687..1704)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1914..1933
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2095..2790
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2228..2246)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2496..2515
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2794..2820
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2794..2820
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3641..3658)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3647..3871
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3917..4345
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4004..4023)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4214..4235
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4354..4374)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4359..4688
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4539..4674
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4601..4620
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4755..5549
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4809..4828)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5419..5438
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5723..5844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5760..5779)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5814..5833
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5893..5909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5893..5909)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5906..5928)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5917..5933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5941..5947,5948..5965,5966..5971))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5986..6007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6124..6141)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6295..6883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6375..6394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7054..7845,7846..7914))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7677..7696
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7915..8019)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8094..8113)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
       61 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      121 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      181 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      241 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      301 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      361 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      421 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      481 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      541 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      601 atataagcag agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta
      661 atacgactca ctatagggag acccaagctt gccaccatgg tgagcaaggg cgaggaggat
      721 aacatggcct ctctcccagc gacacatgag ttacacatct ttggctccat caacggtgtg
      781 gactttgaca tggtgggtca gggcaccggc aatccaaatg atggttatga ggagttaaac
      841 ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg
      901 tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg
      961 gtagatggct ccggatacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt
     1021 actgttaact accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgaag
     1081 gggactggtt tccctgctga cggtcctgtg atgaccaact cgctgaccgc tgcggactgg
     1141 tgcaggtcga agaagactta ccccaacgac aaaaccatca tcagtacctt taagtggagt
     1201 tacaccactg gaaatggcaa gcgctaccgg agcactgcgc ggaccaccta cacctttgcc
     1261 aagccaatgg cggctaacta tctgaagaac cagccgatgt acgtgttccg taagacggag
     1321 ctcaagcact ccaagaccga gctcaacttc aaggagtggc aaaaggcctt taccgatgtg
     1381 atgggcatgg acgagctgta caagtaacgg ctctagatcg cgaacgcgtg aattcctcga
     1441 gggcggccgc tctagagtcg acgggccgcg gtaacaattg ttaactaact taagctagca
     1501 acggtttccc tctagcggga tcaattccgc cccccccccc taacgttact ggccgaagcc
     1561 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     1621 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     1681 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     1741 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     1801 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     1861 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     1921 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     1981 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     2041 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatggtg
     2101 agcaagggcg aggcagtgat caaggagttc atgcggttca aggtgcacat ggagggctcc
     2161 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2221 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttctcctg ggacatcctg
     2281 tcccctcagt tcatgtacgg ctccagggcc ttcatcaagc accccgccga catccccgac
     2341 tactataagc agtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2401 ggcggcgccg tgaccgtgac ccaggacacc tccctggagg acggcaccct gatctacaag
     2461 gtgaagctcc gcggcaccaa cttccctcct gacggccccg taatgcagaa gaagacaatg
     2521 ggctgggaag cgtccaccga gcggttgtac cccgaggacg gcgtgctgaa gggcgacatt
     2581 aagatggccc tgcgcctgaa ggacggcggc cgctacctgg cggacttcaa gaccacctac
     2641 aaggccaaga agcccgtgca gatgcccggc gcctacaacg tcgaccgcaa gttggacatc
     2701 acctcccaca acgaggacta caccgtggtg gaacagtacg aacgctccga gggccgccac
     2761 tccaccggcg gcatggacga gctgtacaag agttacccct acgacgtgcc cgactacgcc
     2821 ggtaccggcg ggagtggcgg tagttccaag actgcgtata gagtaaacct gaaactggat
     2881 caagccgatg tcgtagatag tggggctcct aaagtgaggt atactcaagt ctggtcacat
     2941 gacgtaacga tagtcgctaa ctctacggaa gcgagccgaa aaagcctcta cgatctcacg
     3001 aagtccctcg tggctacttc tcaagtcgag gatctcgtag tcaatcttgt cccgcttggg
     3061 cgcgcggatc cgctagcctc aaagacaata gtgttgagtg tgggggaagc tacgcggacc
     3121 ttgactgaaa tacaacgcac cgcagatcgg cagattttcg aggagaaggt ggggcctgct
     3181 gtcggccgct tgaggcttac cgcttcactg cgacagaacg gagctaaaac cgcctatagg
     3241 gtcaacttga aattggatca ggcagacgtc gttgattctg gggcgccaaa ggtaaggtat
     3301 acccaagtat ggtcccacga cgttacgatt gtagccaatt ctactgaggc ttcacgaaag
     3361 tcactctacg acttgaccaa atctcttgtt gcaacatcac aggtggagga ccttgtcgtc
     3421 aatttggtgc cactcgggcg agcgggcgcc ctagccagta agaccattgt gcttagcgta
     3481 ggggaagcca cacggacgct taccgaaata cagagaacgg ccgaccggca gatttttgaa
     3541 gaaaaagtag gccccgcagt tggacgcctg aggcttacgg cttcccttcg acagaggcgt
     3601 cgaggataat ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     3661 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3721 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3781 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3841 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3901 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3961 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4021 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4081 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4141 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4201 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4261 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4321 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4381 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4441 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4501 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4561 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4621 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4681 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     4741 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4801 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4861 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4921 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4981 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     5041 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5101 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5161 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5221 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5281 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5341 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5401 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5461 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5521 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5581 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5641 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5701 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5761 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5821 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5881 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5941 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6001 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6061 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6121 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6181 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6241 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6301 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6361 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6421 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6481 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6541 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6601 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6661 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6721 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6781 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6841 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6901 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6961 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7021 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7081 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7141 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7201 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7261 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7321 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7381 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7441 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7501 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7561 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7621 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7681 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7741 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7801 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7861 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7921 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7981 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8041 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     8101 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     8161 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     8221 aagaatctgc ttagggttag gcgttttgcg ctgc
//
