LOCUS       sequence-458568-       18234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of the erg8, erg19, idi1, and erg20 genes in 
            Aspergillus oryzae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18234)
  TITLE     Aspergillus oryzae plasmids
REFERENCE   2  (bases 1 to 18234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pwAB-erg8erg19idi1erg20
FEATURES             Location/Qualifiers
     source          1..18234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8354..8368
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             9537..9548
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     RBS             13410..13418
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(16013..16029)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16013..16029)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16026..16048)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16037..16053
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16061..16067,16068..16085,16086..16091))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16106..16127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16244..16261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16415..17003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16495..16514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17174..17965,17966..18034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17797..17816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18035..18139)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     18207..18225
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccttgcct
      421 taatctggtg tgggatggac tttgaatcgt ttcacaatta aattgtagat acttatagaa
      481 tacgtggcga gaaaaactca agtcgtcgcc tcttctcgtc tgtaggcctg ctgtcaagta
      541 tcccatcata ggttgtctcc tcaactgcac atcctcaatc ccaatacagt gcaggtcaaa
      601 gaagatttgg aataaattct tcgagcatcg agatctagct tagcccagtt gtgaggttat
      661 gtatagcgtt tcgtcttgat atatttgtaa ttccacggtc aattaattca ttgagctgtc
      721 attcatctcg cgaaccgaac cgataccatg ccactctgca tactaacggg atgcttcgac
      781 ctttttcagt tcctccctgc agtcaaacca acaatattag taccatgtct cgacgtaata
      841 tcaacagcca caatggcacc gaacgcctag caactgctgg agctgtggcc accacggcca
      901 ccatagggga ctaatacgca cttcaactgc ttcgagaagt cctcagcggc atcatcgtca
      961 tcccagctct cttcccaaag gtgaccatta gcgccgtttg ctgatccaga agcttgttcg
     1021 gtttcttctt gtggccagtc tgtagatgga aaccgtcggt cagcgcaact ctcatctcac
     1081 gaagcaatcg aagcttccag taatgcaatc aggtcgctgg tcttccttgc tattttcacc
     1141 gggcggaatc cactattatt gtctgacctt caacggggaa atcctcgaat tcatcgtctt
     1201 cctccaacac ggggggcttc tgctgttggg gttgctgtga agcttcgggc ttatcgtggg
     1261 attgtgttga gttcgacatg gtgggtgggg gtgagagaac gatgcgtttg gagtattagg
     1321 cgatagacca gatatgtttt caaaagtgga tattcaggaa gggctcgaat gtgagagagc
     1381 cgaagctagt aatcgcccgt ttgggtttgg gggaacacct tcatccatag taagcttgtg
     1441 cgttgttccc aaacacggga cctgctagcc ataaggaatt tttagatagg gattattatg
     1501 atttttgttc tacgtattct attagatttt ttgtttaaat tttgtttttt tgtttttttg
     1561 ttttttttgt tccttgtcta tatgaaaaaa attttcatct acatactacg tagattgtca
     1621 ccgctcagga ggggcaacaa cagatcttac ctaagtacac tagtaaggtc cgagacagta
     1681 agggattgat ctaaatgagg ggcacacagc agcgatcata tgtgaaagaa aaactagaaa
     1741 ggcaaaaaaa gaaaagaaaa agagaagaaa atttttccta ggtatccgta gtctgtcaac
     1801 attgcctctt tgcctcctaa cagcatatgt acggagtatc tgtacgtact ctagatagcg
     1861 agagtaaaaa aaaaaaagat tttcactgtg gaccagacag gcgccactcg gccgggccac
     1921 agctgcttgg gtgttgaccg ggagcggacc aattaaggac tcgaacgacc gcggggttca
     1981 aatgcaaaca agtacaacac gcagcaaacg aagcagccca ccactgcgtt gatgcccagt
     2041 ttgactgtcc gaaatccacc ggaaaggtgg aaacatacta tgtaacaatc agagggaaga
     2101 aaaaattttt atcgacgagg caggatagtg actgatggtg gggtcatggt cgggtctccg
     2161 agcgaaagag aaccaaggaa acaagatcaa cgaggttggt gtacccaaaa ggccgcagca
     2221 acaagagtca tcgcccaaaa gtcaacagtc tggaagagac tccgccgtgc agattctgcg
     2281 tcggtcccgc acatgcgtgg tgggggcatt acccctccat gtccaatgat aagggcggcg
     2341 gtcgagggct taagcccgcc cactaattcg ccttctcgct tgcccctcca tataaggatt
     2401 ccccctcctt cccctcccac aacttttttc cttctttctc tcttcgtccg catcagtacg
     2461 tatatctttc ccccatacct cctttcctac tcttcttcca ttcattcaac tcttctcctt
     2521 actgacatct gttttgctca gtacctctac gcgatcagcc gtagtatctg agcaagcttc
     2581 tctacagaat ctttctagta tcttacaaag aactacaaag ttcgcaccac cttcaaaccc
     2641 atgtcttatc cgccatccgg gagcaccgcc ttgtccgccc cgggcaaggt ccttcttacc
     2701 ggtggttacc tcgttttaga ccgcaattac acggggacag tgatcgcact cgatgctagg
     2761 atccacgtta tcgttcaaca attgaaaaga ggccatcgac gtggtgcatc attcagctca
     2821 gtgaaggggg gtcccgatac ggagacagtc gaggacggaa gtgctgtgga cgacaaggaa
     2881 aaagaagacg tcgtggttgt acgctcgccg caatttgtca atgcaatttg ggagtacggt
     2941 atacagcgtt gtgagaatgg aggtggaatc aaagtgattc aaaggaacga cgggtaagag
     3001 agtgtttttg acctctccag ttcaataact aagggatccc aggcgttcca atccgttcgt
     3061 cgaaacttcc ctcaactacg ctctcaccta tatcagctat gtggccgact cgaaggactt
     3121 tgggtccctc tccgtgacca tcctggccga cactgattac tactctgaga ctgccttctc
     3181 tagggtttct gagtcccctg gaagattcgt gaacttcggt gttcctcttc acgaggccca
     3241 caagacagga ctaggttcct ctgcggctct agtaactgcc ctagtatcat ccctcgttat
     3301 tcaccgtacc ctgcagcctg acgaccttgg agcttctcgt gacaagcttc ataacttggc
     3361 acaggctgcc cactgtgctg ctcaaggtaa agtgggatcc gggtttgatg tggctgctgc
     3421 tatctacggc tcttgcctat atcgccgatt ctccccaagc attttggaat ccgtcggcga
     3481 cgccggttca cctgggtttg aagagcggct gtttgcagtc gtggaggacg ccgaccctaa
     3541 gcatccgtgg gatacagagt gcttggattt cggcatgcga cttcctcgag gcatgcagat
     3601 ggtcctgtgc gatgttgagt gcggctcaaa ttccccttcg atggtcaaga aagtgctcga
     3661 atggcgaaaa caaaaccagc aggaagccga tcttctttgg gctgccctcc agtcaaacaa
     3721 tgaaaggctc tgtcttcagc tcaagcagct agcccagagc cctgaccaag aatcgcccga
     3781 agatttcaat gatgtccgca acctcatcca gcgctcacgc aatcaccttc gcagcatgac
     3841 ccgcaaggcg ggagtcccca ttgagccgcg ggtacagaca gaactgcttg atgccgtgtc
     3901 agccgttgac ggagtgattg gtggtgtggt tcctggtgcg ggagggtatg atgcgattgc
     3961 tgtcttgatc cgcgatgacc aggaggtgct taaaaagtta actgagctct ttaagaactg
     4021 ggaaagtaag gtggaggacg atttcggtgg caagatcgga actgtccggc tcctcggtgt
     4081 gcgtcacggc tcagatggag tcaaaaatga ggttctcgac caatatgccg gttggcttta
     4141 agatgtctct tgatacgatg tctctgccga tacgaagaaa gaaatcagat accagtacat
     4201 aaattgatca atccaagaac ggaaacgaac aaaagaaaca tatttgactg taaaagtgga
     4261 ggccgctttt ggtgactgga aatattgcat gactggggaa gctggagttg aatgattggt
     4321 caattacgat gtatgtagaa gtagtatgag gatgaatcca cttaattaat tgatgcatct
     4381 atacgcgcta tccttctctc aggctttttt actgtcacta gtccatggga tgttgattcg
     4441 tacctgctcc cacgcacgag gtaaggagcc ccactgcagc attgtggcgg tcctcagcct
     4501 tacgaagcac caaactcgat acgtcaatca gtgctaggta ggtacgtctg tactccggcc
     4561 cctggtatcg agacgatgaa tgaagttccg gaccagaatt cccagaatcg cattgaagat
     4621 gtgtttcatt gtgttctatt tggggtccag tgtgatggta tgacgccagc cgggaccgcg
     4681 agccatgcaa ccatgacaag cgcgtgatcc tcatttgcct accggtgaat cttttcagga
     4741 atcaggagag cagaagaggc ttagagagat cgctaagccc atgatatgag tgtcgagagg
     4801 gaagaggggc agtggaccat agttgatccg gtccgatatc tcggcccgga aacggaaagg
     4861 tcacaccgag tgcccctcat ttttccattg cttccatcca ttaagcttgg gtgggatgct
     4921 gtggtctgta gtgttagtct gtatggccag attgtaaatt acatcatgcc cctctatggg
     4981 gatgcctcag gtatgggacc ccagggtatc atttccccct caattgcttg aactacggaa
     5041 caaaggacaa aaagatagag taatagccgg gatcgtcttc ctcgtagcct aggtagtact
     5101 gccccctcga ttccgaaaaa ctggcaaaag attcacgaga tggtaggatt gagtacccgg
     5161 catgctggat ttgaggcacg cttattggcc agaccggtag ctgccgagga gaggcagagt
     5221 cccaaatatc gtgagtctcc tgctttgccc ggtgtatgaa accggaaagg gctgctggga
     5281 gctggggagc ggcgcaagcc gggaaaacag ctgacaagga cccatttcac tctggatctt
     5341 gaggagagct gtagcttttg ccccgtctgt ccacccggtg actggattag tgacctggtc
     5401 gttgcgtcag tcaacattgc tcttttttta tctccccctc ccccgccgtc cgacttttct
     5461 ccccttttct actctcttcg tatactcacc actgcaatca ccttatccct ttgtcttttt
     5521 acttaaagtg agtcgtctcc cgcccatcat tccctttgga tcttcacttt caagtgccta
     5581 ccgtttccct ttccacacag attgactgac agctaccccg ccacaccaac agacacatct
     5641 aaacaatggc tgctccttct gacagtacgg tctttcgggc caccaccaca gccccagtca
     5701 atatcgctgt tatcaagttt gtcttccata cccctgaata gcacgattgt cactaggcta
     5761 cattgatact aatcatggat ctgaagatac tggggtaaaa gggacgccac tctgaacttg
     5821 cctacgaact cctccctctc tgtaaccctc tcgcagcgtt ctttgcgcac cctcaccact
     5881 gcctcatgct ctgccaagta cccgactgcc gacgagctta tcctcaatgg caaacctcaa
     5941 gatatccagt cctccaagcg cacccttgct tgcctttcga atctgcgctc cctccgccag
     6001 gaacttgaag ctgccgactc ttctctgccg agactgtcta cccttcccct acggatcgtt
     6061 tccgagaaca acttccccac cgccgctggc ctcgccagtt ccgccgccgg tttcgcagcg
     6121 ctcgtccgtg ccgtcgccga cctttaccag cttccccagt ccccccgaga cctcagccgc
     6181 atcgctcgtc agggatctgg ttccgcttgt cggtctctga tgggcggata tgtggcctgg
     6241 cgcgccggaa atcttgccga tggtagcgac agcttggctg aggaggttgc tcccgagtca
     6301 cactggcctg agatgcgtgc gctcatcctg gtcgtcagcg ctgaaaagaa ggatgtgccc
     6361 agtacggagg gtatgcaaac caccgttgct acttccaacc tcttcgcgac ccgcgcggaa
     6421 tctgttgtac ccgagcggat ggcggctatt gagactgcca ttcagaaccg agatttccct
     6481 gccttcgccg agattaccat gcgggactcc aatggcttcc acgccacctg tctcgactcc
     6541 tggcctccca tcttctatat gaacgatgtc tctcgcgccg ctgttaggct cgtccacgat
     6601 attaaccgtg ccgtcggtcg tacggtttgc gcttacactt ttgatgctgg tccgaacgcg
     6661 gtcatctact accttgagaa ggattctgaa ctcgttgccg gtaccgtcaa ggctatcctg
     6721 ggcgccagca gtgagggctg ggacggtccg ttctacgaac ctcttaagag cttcaccgct
     6781 ccgggtgtgg cattggataa ggtggactct agggctgttg atgtgctcaa ggatggtgtc
     6841 agtcgtgtga tcttaaccgg cgtcggtgag ggtcctgtca gtgtcaacga ccacctcgtc
     6901 agtgagacag gtgacattct ctccaactaa cgcgatctgt agtagctcgt gaagggtgga
     6961 gagtatatga tggtactgct attcaatctg gcattggaca gtgagtttga gtttgatgta
     7021 cagttggagt cgttactgct gtcatcccct tatactcttc gattgttttt cgaaccctaa
     7081 tgccaagcac gctagtctat tataggaaag gatcccctct tatagttgcg gcgctggctc
     7141 atgattgcca ctgacatgtc acttggacaa caatccagaa tctgcagcac taaaaaggtc
     7201 cacaactcgt gtggccaccc aaagagtggg acaacaggac agcacccgag tctaaggggc
     7261 acactagggg ctatttttgg ccgacaatgg atccccccag attttcactc ttcaaagttc
     7321 acagaccctt ccactaagac caaacgtgcg aggataacat tcaattaggg ccaaccgtca
     7381 tctgactgat cacaaacacc aattgtccag cagaagaagg ccactgtaac attctctccc
     7441 ttacagcatc ccttaacgaa tgatcgcagg caaagtggtg gaagacacga ccactaagtt
     7501 tggagactag accaaagttg caacgtccca agagagccct ggaggcttct cgatcttgcc
     7561 ggcctataaa cttggaaaag tccgccggct atctacaggg tcgctcgatt tgattggttt
     7621 gtcaggggcc ttagccgaag ctctcgtgaa acccgacgaa tcagatcatt ccaatgacga
     7681 gaaaaagagt atccgagttt caatcatacg gcaacaaccc tgatactaaa ttacctttgc
     7741 ttgggattgt cccctgtcgt tttgaaaaaa ggaaaagaaa attgaaattg aaattgaaag
     7801 aaagtaaaat tcaccctaaa ccaaggaaga gtaaaaataa aataaaccta agactgcccc
     7861 ccccgcttta ggtagcttta attaaatccc cgtctttctc ctctgctttc tttctcttcc
     7921 tccttttctc tccacagtcc ttcgttaact acttactaac tacgcgtttc tatacactcg
     7981 ttaatttctt cgagttttat tatcgcttca gcgtctttgt tattttcaaa ccgtcaagat
     8041 ggcggagatt actattttat tcccctttcc ccctcctcaa ggtcggatcg ataagtacaa
     8101 tacccctctt tggattttta ttttgtgtga gcgtcatatt ttagctgtca caatgagcgt
     8161 aactactaca accaccgagc cccctagaat cacggcggag aatgtcgcca ctctcttccc
     8221 agaggtcgat acttctttgg ctcgtgaagt cctcccaaaa gccgatggca atccctccgc
     8281 agctagcagc aacgagctgg cgggttatga cgacgaacag gtccgtctaa tggatgaagt
     8341 atgcatcgtt ctggatgatg atgacaagcc tattggaagt gccagcaaga agacctgtaa
     8401 gataatccct ctacatgact actcgtctgg gttgcagtgt ggttttcctg ctagcgtttt
     8461 gcctcttccc ctctctgcta ataccgttta ctgatggctc atccaattgg tgaaaaatag
     8521 gccacctcat gaccaacatt gaccgcggcc ttctccaccg tgccttttcc gtgttcctct
     8581 tcgattccaa caagcgcttg cttctccaac agcgcgccac tgagaagatt acattcccag
     8641 atatgtggac aaacacttgc tgctctcacc ctcttggaat tgctggcgag accggttctg
     8701 agctggatgc cgctatcttg ggcgtgaagc gggctgcgca gcggaagttg gaacatgagc
     8761 ttggaattaa gccggagcaa gtacccctgg ataagttcga tttcttcacg agaatacatt
     8821 acaaggctcc tagtgatggg aagtggggag agcatgagag tatgtttgat atcctttgac
     8881 attcatttca tgatcaccgg tcgtcgaaaa ctaacattat taagtcgact atattctctt
     8941 catccaggca gatgtagagc tgaagcctag cccgaatgag gttcgagaca cgaagtacgt
     9001 ctcggctgac gaattgaaga cgatgtttga gcagccgggg ttgaaattca cgccttggtt
     9061 caaacttatc tgcaattcga tgttgttcga atggtggagc catctcggct ctccaaccct
     9121 ggagaagtac aagggcgaga aaggtatccg gcgtatgtga atgaaccgta tgattatatg
     9181 acgtctacca gccaacctga cacattatcg aaagatggtt ggactcggga ccacactgca
     9241 tacccgggtt cccgctactt gcaacctctg agctgcacaa agcactacag atgatattct
     9301 cttaagattt atgatgacga ttcgattagg ccagatatag tggcagtaat gcaataaaag
     9361 tgccaaaaaa gtccctgccc atgtagaccg tcgctagtag ctatagtctc ccaatatact
     9421 tcgtagatat ttactttata atgtataatg atgttgactc ctaatgaatg ggcgtgaatg
     9481 gtaagggtac acgtgacccc gctagcccca ccaccagatc gttttatcgt gaagttattg
     9541 agggacggct caccagctca cttaccacca tcggcccatt agctcattac gcagatcctc
     9601 tggcatatta tgcaacccat ggccctattt agatcgcttt gcaatcccag cagcttgcat
     9661 gggtgacaga ttagtcacag aatccctagt gtcactatgg tgaatgaagt ttacgttatg
     9721 aatagcatca agtatacgca ttttggcggg tattgattag tagccacacg caagtaaatc
     9781 gttgttccat gacaggcgga atgagatgta gggctccttt ccaccaatga accgcgaatg
     9841 tgatcgttta tcaaggggac atcccgagga tcgatgcata gatagtacta ttttgagatc
     9901 cggaatataa caacaccgac tgcgtgaagc ttccccacct ttttcaattt tagtattatg
     9961 actattttct tttttagggt cagctgagca gggacaagag ggcctagaca taaaaagacg
    10021 caaaggacag aaagatgccg aacgagttgg aaaggaaaga acgggtacgg ggatgagatc
    10081 tccgccgatt tccccgatgt ttggctatag cacgaaagaa atacggagag ggaatgggtc
    10141 aatcttttag caggagggga gaaccccaga ttcgcgcaac aacaacatca caattcaaat
    10201 ttgtcatgtt gtgtttatga accggttaat gagggtatcc agcacggggg ttgaaggtgg
    10261 atctcataca ggagaattat tatctatttt ctaatttcta cactgcatga atggcatgtc
    10321 ataggtgttt cgggtccatg gaagaagaga aggtgatgat gtcatgtgat ggcggggatt
    10381 ttcgggaggc gcctgggtcc tgttgggcct gaggccagca accagcgtca tcgttgaacc
    10441 ccaccaaaca agtccaagtc gtcacgggaa gaaaagggaa gaaggacacg aacttccatc
    10501 cattaccctc gggcactggg aaatgactaa ctaaatacta cttcttctgc cttgtttctc
    10561 ggcttcttct tctttctcct tttttccctt tttcttatat atcatcataa cccccgcctc
    10621 ttcttctttc ccctcccctc caattctcag catcacgcca gcaaccggtc agtgaaatca
    10681 tactgatacc atccgttccc cactacccgg tgagttcttt cgagtgctgt tgcccctcca
    10741 ttagtggctc agctgcccct cctcgtccct ccctttgcca atgacacttt tattaaatcc
    10801 cctgctccgt attcgtcgtt cggtaaagat ccttagttcg gaagctccta cagaacttat
    10861 ttctagctgt tcacttcctc ctccatcctt tccttctcct cttccatttc ttctcccaaa
    10921 ctaatccaat tcctagtcga gtaagaccag ctatctgatc gaatctttca actatcacaa
    10981 atgcctgcca ctgaccgtgc cgctttccag gaggtcttcc cctccttggc cgaagatatc
    11041 ctggcctatg cgaaggagtc aaacttgccc gaaaatgccc tccagtggtt tgagaaggta
    11101 tactccccgt tttttgcacg attcgctgcg gaggacatgg actgaccact cttgctacgt
    11161 acaacaggcc ctcaatgtta acgttcccgg tggaaagctg aaccgcggtc tttcctgccc
    11221 cgacaccggt ctcgctcttc tggagaagcc cctgaccgag gagcagttca agcacctcag
    11281 cattctggga tggctcaccg agcttctcca ggctttcttc ctggtcagcg atgacattat
    11341 ggacagctcg atcacccgtc gtggtcagcc atgctggtac cgtcaggagg gtgtcggtct
    11401 gatcgccatt aacgatgcct tcctgctcga atcttccatt tatgtcattc tcaagaagca
    11461 cttccgctcg caccccgcct acgccgactt catcgatctc ttccacgaga ctacttggca
    11521 gactgagcta ggtcagctct gtgacctgat taccgccccc gaggacaagg tcgacctcaa
    11581 caacttctcc atggagaagt atatgttcat cgtcacctac aagaccgcct actacagctt
    11641 ctacctgcct gtcgccctgg ctctgctcta ccttcagcgt gccacgccca gcaacctccg
    11701 ccaggcccac gacatcctca tccctctggg ccagtacttc caggttcagg atgactacct
    11761 cgatgcctac ggcgatcctg aagtcattgg taagatcggt accgatatca aggacaacaa
    11821 gtgctcttgg ctcgtcaatc aggctctcca gcgctgcaat gctgagcagc gcaaggtcct
    11881 cgacgaggcc tacggccgta aggatgacgc tcttgaggcc aaggtcaagg ctctctacaa
    11941 ggagctagat ctcgagaagg tctaccaaga gtacgaggag aagatcgtcg gcgagctgaa
    12001 ggagaagatc gctgccgtgg atgagtctgg gggcctgaag aaggaggtct tcaacgcctt
    12061 cctcggcaag atctacaagc gctccaaata aggggctggt taatatgtgc ggggatgcaa
    12121 ttatgaacct tttatgagca caaaaatatg gaattctcat gaaattagac ttcaattatg
    12181 tagttgattc tacagaactt gaatcatgat tttattcgtt aaacttgttg ccatctgtag
    12241 actgtgcact gttcggtaac tgaataactt attagcacga agcccgttag tttaatcacg
    12301 ttcaactgct cctgccggcg gatacaatga cagtatgggt gcacttgatc aatgtatctc
    12361 tgttcctgat gggccatttc aacgcacacg tatcataatt cttagagcca tcaatgatgc
    12421 ttggaggcct ctcccttata gcagtgccta tcctccctga ctgctcgata ttaattgatc
    12481 catctcttca gtttaccacc aacaacctgt acaagtaaca tcatcaatcc ctgcatgtct
    12541 ggcgtggcac accgacaagt gtttggatag tacaaatgtt gtattatatc ttatactaaa
    12601 acactgtcca tgtcagaaaa gtcttgatgg aaatgtgtag tcgcgaaata tgatccctgg
    12661 tggcgcagtg accaaactat cgtgagagat atatatcgta gcttgccact agcgagtagc
    12721 tttacgccaa attacatgac ttccacacaa cagtacactt ccgtcacagg tcagaaaagc
    12781 gtcttgcgga caactttccc tcttcaagtt attctaagta ctagtttgtg tgttggatcc
    12841 tgatcacaga ttgtcgattt aaatcctcat ataaccgtcg cgagttagta ccaaggaatt
    12901 gaacctctcg aactacttga ggatgtacgt actactaagc ccgaccgaca acccggagaa
    12961 tggcaactaa gcagtcctgt tcctactagt agactagtag tagctagcga atttacaggc
    13021 atactgcgtg gaaaaagtaa actgaatgca ccggccccct ttgtggcaat cgacagttat
    13081 gaaaaagctg agctaagccc cagccaccag catatttaca gaggatttca aatgaaagca
    13141 gggaatgctt gttgttttcc tttggggcat ggaagggggc atgggaacga cacgtgacca
    13201 cgacggccat cttcactatt gccgaatgcg gcgacgactc ccgttaacgt tgcaacgacg
    13261 agaacagact ttgctacagt tcgtctctcc caatgcaatt gaatcaaggc caatcatcta
    13321 tgtcattatt cggtggtctg cacgcatatg caaagcttgc ttgccggctg tatgtgccag
    13381 gagcttcgga aactgagttt ggaacaccca cctccttacg gtagcgacgt ggctgacctg
    13441 acgtgtaccg cagattgtct ctcagggagg caaattcttg agtctagaat tgtatatata
    13501 cagtgtcaat atgattcaag gtggatgtca gcggtctcaa gagcgtaacg gtaggattcg
    13561 ccagatcgat tctcagacca tattggcgat tgagcacaca actcccaatg gagacgatcg
    13621 ttatcgctcg catgatatgg tcttggcttc ccaataatat taaaagactc tagaagattt
    13681 tgacaatgtg acctcattca tttggctgga aacgagcttg tgattgccct ttgagtgatc
    13741 ggtggaatgc tcgtccaagt tctttgcatg ccgtaattga gacatagtct ccactgtcgc
    13801 atgtggtagt aactctatct tggtataaat catacttaat atctagccaa tcgctttggg
    13861 aaaacaattt gatcagcatc tctgcgactc ttgactggca ctttcttgac ccaacatgga
    13921 ggggccacgc ggcgtctatc tcttcggaga ccagacaagt gatttcgacg ccggcttacg
    13981 tcgcctccta caagtaaaga ataacacaat tgttgcatcg ttcttccaga gatgctttca
    14041 cgctttgcgc caagagatcg cgaggctttc accatctgaa cggaagatct tcccccggtt
    14101 tacgagcata gtggatctac tggcgcgtca ccgggagtca gaccctaatc cggctctgga
    14161 gagtgcgttg acctgtatct atcaattggg atgctttata aagtacgtgt aactgcagat
    14221 cctgacccgt ttgaacgagc ctaacctgag atagctacta cggagacctt ggaaacgtgt
    14281 acccatctgc ttcagactgc catatagttg gcctgtgcgc gggtcttctt agttctgcag
    14341 ctgtaagctg ttcgaacaat gttggagaat tgctccccgc tgcggttgaa gcggtggtgg
    14401 tagctctccg acttggtcta tgcgtcctta aagttcgaga gctggtgagc tctgaccaag
    14461 cgtcgtcaac aagctggtca gtcttgattt cagggattag cgagaaagat gcctcgcagc
    14521 ttataggaga attcactgct gaacgggtaa gtcaattgat ctgaaatagt ttgcaggaca
    14581 gaatgttcta accactggat aaaggcaatt cctccttcat ccaaaccgta tatcagtgcg
    14641 gtgggatata acagtataac catcagcgca ccgcctaagg tccttgatga tttaattgat
    14701 tctaggctgt ctaagagcca taagccggtg agggcgcaaa tccatggtcc ttaccatgca
    14761 gcacatctgt actatggccg agatgtcgac aggatcatcg aaagctgcca taatgaggtc
    14821 gtttcaaact acacaccccg tatccccgta ctatcaagta ctacgggaca gccgatagag
    14881 gccaaacaca tgaaagatct acttaaggcc gcccttgaag agattctact acgtcaacta
    14941 tgctgggaga aagtgaccga tgcctgctat tccatattaa aaactgctcg tcatcaacca
    15001 tgcaagttgt tcccaatttc aagcactgcg acacaaagct tgtttacagc tcttacgaaa
    15061 gccgggataa ccgacatcga agtggaaaat gggctcggag atgttcccac gaacccgaag
    15121 gacaacctta acatcagcgg cagggcggac tgctccaaga tagctatcat tggcatgtct
    15181 ggacgattcc cagaagctga tggcacagag agtttctggg accttctgta taatggcctc
    15241 gatgtacacc ggaaggtgcc tgcagagcgt tgggatgttg atgcccacgt tgatcctacc
    15301 ggaacaaaac ggaacaccag caaggttcca tacggatgct ggataaacga accggggtta
    15361 tttgaccccc gcttcttcaa tatgtcgcca cgcgaagccc tccaggcaga tcccgctcaa
    15421 agacttgcat tgctcacggc ctatgaagct cttgaaatgg ccggctttat ccccgacagc
    15481 accccttcta cacagaggga tcgagtcggc ctcttctatg gaatgactag cgatgactat
    15541 cgggagataa atagtggtca agatattgat acttacttta tccctggtgg gaatcgtgct
    15601 ttcacacctg gccggataaa ctactatttc aagttcagtg ggcccagcgt cagcgttgat
    15661 acagcttgtt cttcaagtct tgcggctatt catatggctt gcaattcgat ctggagaaat
    15721 gattgcgatg ctgctattgc tggaggtgtc aatatattga caaaccctga taaccatgcc
    15781 ggtcttgacc gtggccattt cctgtccaga accgggaatt gcaacacatt tgacgatggt
    15841 gctgatggct actgtagagc agatggagtg ggtacaatca ttctcaagcg gctggaagac
    15901 gctcaggcgg acaacgatcc aatcctcggt gtgatcaatg gagcctatac caatcattcg
    15961 gcggggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc
    16021 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    16081 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    16141 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    16201 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    16261 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    16321 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    16381 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    16441 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    16501 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    16561 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    16621 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    16681 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    16741 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    16801 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    16861 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    16921 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    16981 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    17041 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    17101 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    17161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    17221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    17281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    17341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    17401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    17461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    17521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    17581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    17641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    17701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    17761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    17821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    17881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    17941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    18001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    18061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    18121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    18181 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
