LOCUS       sequence-458630-       14702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Flag-tagged wild-type UBR5 over-expression construct for transient 
            expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14702)
  AUTHORS   Mark KG, Kolla S, Aguirre JD, Garshott DM, Schmitt S, Haakonsen DL, 
            Xu C, Kater L, Kempf G, Martinez-Gonzalez B, Akopian D, See SK, 
            Thoma NH, Rape M
  TITLE     Orphan quality control shapes network dynamics and gene expression.
  JOURNAL   Cell. 2023 Aug 3;186(16):3460-3475.e23. doi: 
            10.1016/j.cell.2023.06.015. Epub 2023 Jul 20.
  PUBMED    37478862
REFERENCE   2  (bases 1 to 14702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.06.015"; journalName: "Cell"; date: "2023-08-3-
            3"; volume: "186"; issue: "16"; pages: "3460-3475.e23"
COMMENT     Sequence Label: pCMV 3xFLAG-UBR5
FEATURES             Location/Qualifiers
     source          1..14702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             956..1021
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    9486..10108
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(9685..9704)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(10132..10152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10150..10447
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             10501..11295
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10555..10574)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11165..11184
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11947..12081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11984..12003)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12038..12057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12086..12105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12087..12105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12119..12135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12119..12135)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12132..12154)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12143..12159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12167..12173,12174..12191,12192..12197))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12212..12233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12350..12367)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12521..13109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12601..12620)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13280..14071,14072..14140))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13903..13922
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14141..14245)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14272..14700
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14359..14378)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14569..14590
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaattaa ccatggacta
      961 caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
     1021 gcttgcggcc gcgaattcag gccggcccat gacgtccatc catttcgtgg ttcacccgct
     1081 gccgggcacc gaggaccagc tcaatgacag gttacgagaa gtttctgaga agctgaacaa
     1141 atataattta aacagccacc cccctttgaa tgtattggaa caggctacta ttaaacagtg
     1201 tgtggtggga ccaaatcatg ctgcctttct tcttgaggat ggtagagttt gcaggattgg
     1261 tttttcagta cagccagaca gattggaatt gggtaaacct gataataatg atgggtcaaa
     1321 gttgaacagc aactcggggg cagggaggac gtcaaggcct ggtaggacaa gcgactctcc
     1381 atggtttctc tcaggttctg agactctagg caggctggca ggcaacacct taggaagccg
     1441 ctggagttct ggagtgggtg gaagtggtgg aggatcctct ggtaggtcat cagctggagc
     1501 tcgagattcc cgccggcaga ctcgagttat tcggacagga cgggatcgag ggtctgggct
     1561 tttgggcagt cagccccagc cagttattcc agcatctgtc attccagagg agctgatttc
     1621 acaggcccaa gttgttttac aaggcaaatc cagaagtgtc attattcgag aacttcagag
     1681 aacaaatctt gatgtgaacc ttgctgtaaa taatttactt agccgggatg atgaagatgg
     1741 agatgatggg gatgatacag ccagcgaatc ttatttgcct ggagaggatc ttatgtctct
     1801 ccttgatgcc gacattcatt ctgcccaccc aagtgtcatt attgatgcag atgccatgtt
     1861 ttctgaagac attagctatt ttggttaccc ttcttttcgt cgttcatcac tttccaggct
     1921 aggctcatct cgagttctcc ttcttccctt agagagagac tctgagctgt tgcgtgaacg
     1981 tgaatccgtt ttacgtttac gtgaacgaag gtggcttgat ggagcctcat ttgataatga
     2041 aaggggttct accagcaagg aaggagagcc aaacttggat aagaagaata cacctgttca
     2101 aagtccagta tctctaggag aagatttgca gtggtggcct gataaggatg gaacaaaatt
     2161 catctgtatt ggggctctgt attctgaact tctggctgtc agcagtaaag gagaacttta
     2221 tcagtggaaa tggagtgaat ctgagcctta cagaaatgcc cagaatcctt cattacatca
     2281 tccacgagca acatttttgg ggttaaccaa tgaaaagata gtcctcctgt ctgcaaatag
     2341 cataagagca actgtagcta cagaaaataa caaggttgct acatgggtgg atgaaacttt
     2401 aagttctgtg gcttctaaat tagagcacac tgctcagact tactctgaac ttcaaggaga
     2461 gcggatagtt tctttacatt gctgtgccct ttacacctgc gctcagctgg aaaacagttt
     2521 atattggtgg ggtgtagttc cttttagtca aaggaagaaa atgttagaga aagctagagc
     2581 aaaaaataaa aagcctaaat ccagtgctgg tatttcttca atgccgaaca tcactgttgg
     2641 tacccaggta tgcttgagaa ataatcctct ttatcatgct ggagcagttg cattttcaat
     2701 tagtgctggg attcctaaag ttggtgtctt aatggagtca gtttggaata tgaatgacag
     2761 ctgtagattt caacttagat ctcctgaaag cttgaaaaac atggaaaaag ctagcaaaac
     2821 tactgaagct aagcctgaaa gtaagcagga gccagtgaaa acagaaatgg gtcctccacc
     2881 atctccagca tccacgtgta gtgatgcatc ctcaattgcc agcagtgcat caatgccata
     2941 caaacgacga cggtcaaccc ctgcaccaaa agaagaggaa aaggtgaatg aagagcagtg
     3001 gtctcttcgg gaagtggttt ttgtggaaga tgtcaagaat gttcctgttg gcaaggtgct
     3061 aaaagtagat ggtgcctatg ttgctgtaaa atttccagga acctccagta atactaactg
     3121 tcagaacagc tctggtccag atgctgaccc ttcttctctc ctgcaggatt gtaggttact
     3181 tagaattgat gaattgcagg ttgtcaaaac tggtggaaca ccgaaggttc ccgactgttt
     3241 ccaaaggact cctaaaaagc tttgtatacc tgaaaaaaca gaaatattag cagtgaatgt
     3301 agattccaaa ggtgttcatg ctgttctgaa gactggaaat tgggtacgat actgtatctt
     3361 tgatcttgct acaggaaaag cagaacagga aaataatttt cctacaagca gcattgcttt
     3421 ccttggtcag aatgagagga atgtagccat tttcactgct ggacaggaat ctcccattat
     3481 tcttcgagat ggaaatggta ccatctaccc aatggccaaa gattgcatgg gaggaataag
     3541 ggatcccgat tggctggatc ttccacctat tagtagtctt ggaatgggtg tgcattcttt
     3601 aataaatctt cctgccaatt caacaatcaa aaagaaagct gctgttatca tcatggctgt
     3661 agagaaacaa accttaatgc aacacattct gcgctgtgac tatgaggcct gtcgacaata
     3721 tctaatgaat cttgagcaag cggttgtttt agagcagaat ctacagatgc tgcagacatt
     3781 catcagccac agatgtgatg gaaatcgaaa tattttgcat gcttgtgtat cagtttgctt
     3841 tccaaccagc aataaagaaa ctaaagaaga agaggaagcg gagcgttctg aaagaaatac
     3901 atttgcagaa aggctttctg ctgttgaggc cattgcaaat gcaatatcag ttgtttcaag
     3961 taatggccca ggtaatcggg ctggatcatc aagtagccga agtttgagat tacgggaaat
     4021 gatgagacgt tcgttgagag cagctggttt gggtagacat gaagctggag cttcatccag
     4081 tgaccaccag gatccagttt caccccccat agctccccct agttgggttc ctgaccctcc
     4141 tgcgatggat cctgatggtg acattgattt tatcctggcc cccgctgtgg gatctcttac
     4201 cacagcagca accggtactg gtcaaggacc aagcacctcc actattccag gtccttccac
     4261 agagccatct gtagtagaat ccaaggatcg aaaggcgaat gctcatttta tattgaaatt
     4321 gttatgtgac agtgtggttc tccagcccta tctacgagaa cttctttctg ccaaggatgc
     4381 aagagggatg accccattta tgtcagctgt aagtggccga gcttatcctg ctgcaattac
     4441 catcttagaa actgctcaga aaattgcaaa agctgaaata tcctcaagtg aaaaagagga
     4501 agatgtattc atgggaatgg tttgcccatc aggtaccaac cctgatgact ctcctttata
     4561 tgttttatgt tgtaatgaca cttgcagttt tacatggact ggagcagagc acattaacca
     4621 ggatattttt gagtgtcgaa cttgtggctt gctggagtca ctgtgttgtt gtacggaatg
     4681 tgcaagggtt tgtcataaag gtcatgattg caaactcaaa cggacatcac caacagccta
     4741 ctgtgattgt tgggagaaat gtaaatgtaa aactcttatt gctggacaga aatctgctcg
     4801 tcttgatcta ctttatcgcc tgctcactgc tactaatctg gttactctgc caaacagcag
     4861 gggagagcac ctcttactat tcttagtaca gacagtcgca aggcagacgg tggagcattg
     4921 tcaatacagg ccacctcgaa tcagggaaga tcgtaaccga aaaacagcca gtcctgaaga
     4981 ttcagatatg ccagatcatg atttggagcc tccaagattt gcccagcttg cattggagcg
     5041 tgttctacag gactggaatg ccttgaaatc tatgattatg tttgggtcgc aggagaataa
     5101 agaccctctt agtgccagca gtagaatagg ccatcttttg ccagaagagc aagtatacct
     5161 caatcagcaa agtggcacaa ttcggctgga ctgtttcact cattgcctta tagttaagtg
     5221 tacagcagat attttgcttt tagatactct actaggtaca ctagtgaaag aactccaaaa
     5281 caaatataca cctggacgta gagaagaagc tattgctgtg acaatgaggt ttctacgttc
     5341 agtggcaaga gtttttgtta ttctgagtgt ggaaatggct tcatccaaaa agaaaaacaa
     5401 ctttattcca cagccaattg gaaaatgcaa gcgtgtattc caagcattgc taccttacgc
     5461 tgtggaagaa ttgtgcaacg tagcagagtc actgattgtt cctgtcagaa tggggattgc
     5521 tcgtccaact gcaccattta ccctggctag tactagcata gatgccatgc agggcagtga
     5581 agaattattt tcagtggaac cactaccacc acgaccatca tctgatcagt ctagcagctc
     5641 cagtcagtct cagtcatcct acatcatcag gaatccacag cagaggcgca tcagccagtc
     5701 acagcccgtt cggggcagag atgaagaaca ggatgatatt gtttcagcag atgtggaaga
     5761 ggttgaggtg gtggagggtg tggctggaga agaggatcat catgatgaac aggaagaaca
     5821 cggggaagaa aatgctgagg cagagggaca acatgatgag catgatgaag acgggagtga
     5881 tatggagctg gacttgttag cagcagctga aacagaaagt gatagtgaaa gtaaccacag
     5941 caaccaagat aatgctagtg ggcgcagaag cgttgtcact gcagcaactg ctggttcaga
     6001 agcaggagca agcagtgttc ctgccttctt ttctgaagat gattctcaat cgaatgactc
     6061 aagtgattct gatagcagta gtagtcagag tgacgacata gaacaggaga cctttatgct
     6121 tgatgagcca ttagaaagaa ccacaaatag ctcccatgcc aatggtgctg cccaagctcc
     6181 ccgttcaatg cagtgggctg tccgcaacac ccagcatcag cgagcagcca gtacagcccc
     6241 ttccagtaca tctacaccag cagcaagttc agcgggtttg atttatattg atccttcaaa
     6301 cttacgccgg agtggtacca tcagtacaag tgctgcagct gcagcagctg ctttggaagc
     6361 tagcaacgcc agcagttacc taacatctgc aagcagttta gccagggctt acagcattgt
     6421 cattagacaa atctcggact tgatgggcct tattcctaag tataatcacc tagtatactc
     6481 tcagattcca gcagctgtga aattgactta ccaagatgca gtaaacttac agaactatgt
     6541 agaagaaaag cttattccca cttggaactg gatggtcagt attatggatt ctactgaagc
     6601 tcaattacgt tatggttctg cattagcatc tgctggtgat cctggacatc caaatcatcc
     6661 tcttcacgct tctcagaatt cagcgagaag agagaggatg actgcgcgag aagaagctag
     6721 cttacgaaca cttgaaggca gacgacgtgc caccttgctt agcgcccgtc aaggaatgat
     6781 gtctgcacga ggagacttcc taaattatgc tctgtctcta atgcggtctc ataatgatga
     6841 gcattctgat gttcttccag ttttggatgt ttgctcattg aagcatgtgg catatgtttt
     6901 tcaagcactt atatactgga ttaaggcaat gaatcagcag acaacattgg atacacctca
     6961 actagaacgc aaaaggacgc gagaactctt ggaactgggt attgataatg aagattcaga
     7021 acatgaaaat gatgatgaca ccaatcaaag tgctactttg aatgataagg atgatgactc
     7081 tcttcctgca gaaactggcc aaaaccatcc atttttccga cgttcagact ccatgacatt
     7141 ccttgggtgt atacccccaa atccatttga agtgcctctg gctgaagcca tccccttggc
     7201 tgatcagcca catctgttgc agccaaatgc tagaaaggag gatctttttg gccgtccaag
     7261 tcagggtctt tattcttcat ctgccagtag tgggaaatgt ttaatggagg ttacagtgga
     7321 tagaaactgc ctagaggttc ttccaacaaa aatgtcttat gctgccaatc tgaaaaatgt
     7381 aatgaacatg caaaaccggc aaaaaaaaga aggggaagaa cagcccgtgc tgccagaaga
     7441 aactgagagt tcaaaaccag ggccatctgc tcatgatctt gctgcacaat taaaaagtag
     7501 cttactagca gaaataggac ttactgaaag tgaagggcca cctctcacat ctttcaggcc
     7561 acagtgtagc tttatgggaa tggttatttc ccatgatatg ctgctaggac gttggcgcct
     7621 ttctttagaa ctgttcggca gggtattcat ggaagatgtt ggagcagaac ctggatcaat
     7681 cctaactgaa ttgggtggtt ttgaggtaaa agaatcaaaa ttccgcagag aaatggaaaa
     7741 actgagaaac cagcagtcaa gagatttgtc actagaggtt gatcgggatc gagatcttct
     7801 cattcagcag actatgaggc agcttaacaa tcactttggt cgaagatgtg ctactacacc
     7861 aatggctgta cacagagtaa aagtcacatt taaggatgag ccaggagagg gcagtggtgt
     7921 agcacgaagt ttttatacag ccattgcaca agcattttta tcaaatgaaa aattgccaaa
     7981 tctagagtgt atccaaaatg ccaacaaagg cacccacaca agtttaatgc agagattaag
     8041 gaaccgagga gagagagacc gggaaaggga gagagaaagg gaaatgagga ggagtagtgg
     8101 tttgcgagca ggttctcgga gggaccggga tagagacttt agaagacagc tttccatcga
     8161 cactaggccc tttagaccag cctctgaagg gaatcctagc gatgatcctg agcctttgcc
     8221 agcacatcgg caggcacttg gagagaggct ttatcctcgt gtacaagcaa tgcaaccagc
     8281 atttgcaagt aaaatcactg gcatgttgtt ggaattatcc ccagctcagc tgcttctcct
     8341 tctagcaagt gaggattctc tgagagcaag agtggatgag gccatggaac tcattattgc
     8401 acatggacgg gaaaatggag ctgatagtat cctggatctt ggattagtag actcctcaga
     8461 aaaggtacag caggaaaacc gaaagcgcca tggctctagt cgaagtgtag tagatatgga
     8521 tttagatgat acagatgatg gtgatgacaa tgcccctttg ttttaccaac ctgggaaaag
     8581 aggattttat actccaaggc ctggcaagaa cacagaagca aggttgaatt gtttcagaaa
     8641 cattggcagg attcttggac tatgtctgtt acagaatgaa ctctgtccta tcacattgaa
     8701 tagacatgta attaaagtat tgcttggtag aaaagtcaat tggcatgatt ttgctttttt
     8761 tgatcctgta atgtatgaga gtttgcggca actaatcctc gcgtctcaga gttcagatgc
     8821 tgatgctgtt ttctcagcaa tggatttggc atttgcaatt gacctgtgta aagaagaagg
     8881 tggaggacag gttgaactca ttcctaatgg tgtaaatata ccagtcactc cacagaatgt
     8941 atatgagtat gtgcggaaat acgcagaaca cagaatgttg gtagttgcag aacagccctt
     9001 acatgcaatg aggaaaggtc tactagatgt gcttccaaaa aattcattag aagatttaac
     9061 ggcagaagat tttaggcttt tggtaaatgg ctgcggtgaa gtcaatgtgc aaatgctgat
     9121 cagttttacc tctttcaatg atgaatcagg agaaaatgct gagaagcttc tgcagttcaa
     9181 gcgttggttc tggtcaatag tagagaagat gagcatgaca gaacgacaag atcttgttta
     9241 cttttggaca tcaagcccat cactgccagc cagtgaagaa ggattccagc ctatgccctc
     9301 aatcacaata agaccaccag atgaccaaca tcttcctact gcaaatactt gcatttctcg
     9361 actttacgtc ccactctatt cctctaaaca gattctcaaa cagaaattgt tactcgccat
     9421 taagaccaag aattttggtt ttgtgtgagg cgcgccttcg gtaccagtcg actctagagg
     9481 atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
     9541 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
     9601 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
     9661 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
     9721 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
     9781 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
     9841 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
     9901 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
     9961 ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg
    10021 gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg
    10081 ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttggctg tggaatgtgt
    10141 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    10201 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
    10261 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
    10321 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    10381 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    10441 tttttggagg aattgatcag cttgggatct gatcaagaga caggatgagg atcgtttcgc
    10501 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
    10561 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
    10621 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
    10681 caggacgagg cagcgcggct atcgtggctg gcgacgacgg gcgttccttg cgcagctgtg
    10741 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
    10801 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
    10861 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
    10921 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
    10981 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
    11041 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
    11101 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
    11161 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
    11221 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
    11281 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
    11341 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    11401 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    11461 cccaccccgg gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc
    11521 tcgcggagtt ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc
    11581 cgcgcatcca tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcgacc
    11641 cggacgggac gctcctgcgc ctgatacaga acgaattgct tgcaggcatc tcatgagtgt
    11701 gtcttcccgt tttccgcctg aggtcactgc gtggatggag cgctggcgcc tgctgcgcga
    11761 cggcgagctg ctcaccaccc actcgccaag ctggaaccgt aaaaaggccg cgttgctggc
    11821 gtttttccat aggctccgcc gatcataatc agccatacca catttgtaga ggttttactt
    11881 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    11941 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    12001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    12061 gtatcttatc atgtctggat caattcccta tagtgagtcg tattaaattc gtaatcatgt
    12121 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    12181 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    12241 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    12301 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    12361 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    12421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    12481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    12541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    12601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    12661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    12721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12961 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    13021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    13081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    13141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    13201 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    13261 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    13321 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    13381 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    13441 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    13501 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    13561 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    13621 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    13681 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    13741 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    13801 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    13861 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    13921 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    13981 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    14041 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    14101 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    14161 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    14221 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
    14281 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    14341 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    14401 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    14461 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    14521 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    14581 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    14641 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    14701 tt
//
