LOCUS       sequence-458639-       13322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tracing of CRISPR activity in whole animals..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13322)
  AUTHORS   Port F, Buhmann MA, Zhou J, Stricker M, Vaughan-Brown A, Michalsen 
            A-C, Roßmanith E, Poltl A, Großkurth L, Huber J, Menendez Kury LB, 
            Weberbauer B, Hubl M, Heigwer F, Boutros M
  TITLE     Enhanced in vivo gene knockout with undetectable off-targets using 
            multiplexed Cas12a sgRNAs
  JOURNAL   bioRxiv 2024.11.26.625385
REFERENCE   2  (bases 1 to 13322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.26.625385"
COMMENT     Sequence Label: pGRACE
FEATURES             Location/Qualifiers
     source          1..13322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        69..173
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(174..242,243..1034)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(392..411)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1205..1793
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1694..1713
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1947..1964
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2081..2102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2117..2122,2123..2140,2141..2147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2155..2171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2160..2182
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2179..2195
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2179..2195
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2216..2234
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2453..2522
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    2551..2685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2575..2594)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2629..2648
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2957..2977)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          3107..3172
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(join(3298..4008,4009..4011,4012..4014))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3340..3361)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3689..3708
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3948..3969
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(4123..4143)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(5962..8472)
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     protein_bind    complement(8543..8576)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(9351..9965,10036..10167,10371..10686,
                     10748..11402,11477..11750,12152..12223))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     12675..12693
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     complement(12726..12745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12727..12745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12752..12769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12752..12768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      join(12910..13322,1..43)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12997..13016)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13207..13228
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
       61 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      121 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      181 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      241 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      301 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      361 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      421 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      481 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      541 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      601 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      661 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
      721 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      781 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      841 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      901 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      961 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1021 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1081 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1141 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1201 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1261 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1321 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1381 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1441 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1501 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1561 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1621 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1681 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1741 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1801 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1861 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     1921 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     1981 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2041 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     2101 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     2161 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta
     2221 accctcacta aagggaacaa aagctggcta gaactagtgt cgacatgccc gccgtgaccg
     2281 tcgagaaccc gctgacgctg ccccgcgtat ccgcacccgc cgacgccgtc gcacgtcccg
     2341 tgctcaccgt gaccaccgcg cccagcggtt tcgagggcga gggcttcccg gtgcgccgcg
     2401 cgttcgccgg gatcaactac cgccacctcg acccgttcat catgatggac cagatgggtg
     2461 aggtggagta cgcgcccggg gagcccaagg gcacgccctg gcacccgcac cgcggcttcg
     2521 agaccgtgac ctacatcgtc gacactagtg gatccagaca tgataagata cattgatgag
     2581 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     2641 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     2701 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     2761 ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac atcaaatatt
     2821 tttccataat tttcttgtat agcagtgcag ctttttcctt tgtggtgtaa atagcaaagc
     2881 aagcaagagt tctattacta aacacagcat gactcaaaaa acttagcaat tctgaaggaa
     2941 agtccttggg gtcttctacc tttctcttct tttttggagg agtagaatgt tgagagtcag
     3001 cagtagcctc atcatcacta gatggcattt cttctgagca aaacaggttt tcctcattaa
     3061 aggcattcca ccactgctcc cattcatcag ttccataggt tggaatctaa aatacacaaa
     3121 caattagaat cagtagttta acacattata cacttaaaaa ttttatattt accttagagc
     3181 tttaaatctc tgtaggtagt ttgtccaatt atgtcacacc acagaagtaa ggttccttca
     3241 caaagatcct ctagaggtac cctcgagtta ttcctgttta cgcttctcgg gccgcggctt
     3301 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
     3361 gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt
     3421 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
     3481 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
     3541 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     3601 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     3661 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     3721 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     3781 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
     3841 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
     3901 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
     3961 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtgaa
     4021 ttcttagcta gattcctatt aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     4081 agggttttcc cagtcacgac gttgtaaaac gacgggatct ttcaccttgc gcttcttctt
     4141 ggggattccg gagttccctg gaaaccgggg aataccagcc atggtggatg ggagttccct
     4201 ggaaaccggg gatcgatcct gtaagctgca atggaaagaa tgcggatttc ccattagttt
     4261 ttggttacct tatttggtaa tgtagaaatg gcaaaaaaaa aactgagaat ttgcgtggtt
     4321 tccttggact gcgctggtct accaaagtac aattgtcata acaaactaac ttgtttttcg
     4381 tgtacaatta aaaatcactc aatgcaatgt acgttttttt agtacaaaat tttaatcttg
     4441 gacaaaaaaa aatttcataa aaaaatactt ttccgattga aaactgattt tcacttgcga
     4501 tcgaattgta ataagaaatt aaaagaacag ttacaaaata gaaggccccg ccccgaaaaa
     4561 ttaaaaccac aatacgaata tgccgaaaag ggaggggaga agcttctaga cgacacttaa
     4621 cgaatgccgg tgcaaaagaa agccgcaaag agagcacagt tgcctgtgtg tatgcgccct
     4681 taaaatggcg gctgcctaaa actcgaattg gaacccgaaa ttaacattac aaaataaaaa
     4741 aaaaaaaaaa cactaaacgc actctagaaa atttaacaat taagagccac gaaacttttc
     4801 aaagctagtt aaactttacc tttttgttcg atttaacaaa ttcaaggcgt gaaaactact
     4861 gtaaacgcaa gtggcgaggc tgagctaagc gaccgcacgg tttgaaagga atgactggaa
     4921 taaaccgact gaaagtggct ggtgaatgtt gaatgccagt taactgcgtt tttcaatcgc
     4981 tgcctgcgtc gacgtcgcca taggcgtcgc tcgctctcgc catctcgctc gctccgcgcc
     5041 atggtcgcta tgggtgcgaa ggagagcgct ggtgtgttga attgcggcgg ccaagttttg
     5101 tgaccatata tggtcatgtt tcgaggcgaa aagcaaaccg tcgtctggct ttcgctctct
     5161 cacacacaca tacactctct ctcgttattt tgcgcgtgag tgtgtgtata tgaacgaatg
     5221 ctcgaaggtt ttctccattt ttgcaactac aactttgaaa aaccggcaaa aatttctgct
     5281 tcagccaaaa cggctgccta cgtcactatt ttcaagaacc tgatctagtt ttacagatta
     5341 taatatgcat atactgtatg tatgtacatt tatggatgat ctggtgtttt agggcgtggg
     5401 aaaaaaagaa agtgttggcg aattgatttg caattcgtaa tttggcatat ctttgggtga
     5461 aatcgtgaca cgcccacatc agcgagcttg gctttaaaca ctatattaga tcctaacatt
     5521 tgagttgggg ctattgaatt gctttggttt ttataatttc gtataatttg tatgtatata
     5581 tcctgaaatc tgcagttgta atcctgattc ttgagttatg gtatttgaaa ccctggtttt
     5641 gttaattttg atgtctttcg acctattggt attttgattg acaatttagg gccaaacttg
     5701 ggtatttttg gagttttggt caatacaata actctttagc tcgtggctag tattttttga
     5761 tcactttcag cactggcaca taaaaaaatg ttttgtttac aaaaaaaatc acacattttc
     5821 cgcggctttt ctcgattatt gctttttctt ggtgggagtg gttcgatggt gttttgttgt
     5881 tgggaagggg aaagctactt acatttggtg tgttttatta aaattaatta aataaatata
     5941 aattaagtct ttcggtttgg tgtctctgga ttagacgact gctggctgat ggagcggctt
     6001 tgtgtcggga ggagtatcca cacagcacaa gaactcaaac ggtagtgata tgaaaactgg
     6061 tcccgcagcc cgttttataa agcccgctta gcgctaaaac cttatcagcc gcaatgctct
     6121 ccgctctcaa gtcgcgttca aaacttttac catatatggg tatgaattgt tcgccgattg
     6181 ggttttcatt tacggagaat ttcctccgca actgggtgtt cgtgcttgtg tgtgtcttgt
     6241 atatcgccat gaagagtgta cttccatggg tcctatcagt tgcggcatgt gattcaattt
     6301 gggggttact catattcgat acttcagctg gttatcttta tgctgtgatg gtcacggtgc
     6361 ttttacacat tttttcccca actactcatt gtatgccaaa acatacatat gtacatgtgt
     6421 ttatagtggg tttattagta atgtccagcg atattaaaac attacagtta aataatattc
     6481 atattagctt acgtttactg ctatctgcac ttcaaattgg ttattccagg gacattttgt
     6541 gcaacacaca tcttctagta gggcattcac tagtcgtaga aaacacacct aacttaagcc
     6601 ggttttaaca ataacggcga aacagctgcc agacgagaga aaagtacaca gcaagaagca
     6661 tttctataat gatgccgtcc taaatatgca gcatttttgc tcaaattgat catttcgttg
     6721 catcgctcag gtggtatagt atttggctat tgaatgtgat tgctatctta atagttttga
     6781 atagtaatag tgcaaatatt cattcataaa aaataggtaa tcgatttttt cagtgcagtc
     6841 tatgcgtttg cgcaacagag acgaagagag agacgcctca cacacaagca catgcggtct
     6901 aagctgctct ccctcttttt ctctctctct ctggcttgcg gcagttttcc agaggctgtg
     6961 actcagtaag catttcttgt tgttacccta acgataagcg tccgattcct aattctcttt
     7021 caaataattt gttttatttt ttttttttgt gtgttttgaa gtcaaacagc ttcataaaca
     7081 atattaaata tcgcaattgt tattgcaaat gcattcgtat caggcgcctt gatgtttttc
     7141 gccaaaactt cgtctgctct ttgtcagcgt aattgcgttt gccggtcgtg ccgtcttgct
     7201 cgcacgcggc ttgcaaacaa aaagtgaaag agggaaagcc acctgtgttg ttgcttttgt
     7261 tgctatcgtt ggctgcacac acatggcaat aagtaaccat attgtagact tatttatttt
     7321 agcgttttcg ttgactaatc tttgtttaaa taattgccta tttcgagcca aaatgacgca
     7381 taaaaggaaa cggcaacatg cggtgcgttg agcatgcgta agcgagatgg cgctagagag
     7441 agcgcagcat aaaaagaaaa aaacaaccgg cgagtggcga gacacgaata atacacacac
     7501 gcacacatgg tcaaaggagt ggctccaacg tcggtgacgt cgcgcccagc cacagacccc
     7561 gccacgcccc gccccaccct tccttccgct gccccgcacc cctttccttc cactaccgtt
     7621 ttgccgcatt tggtattgcc atctcgcttt tttggcttta ctttgctttt tgaataagcc
     7681 aagaaactga aatcaaagcg gacagtccga aaattgaata ttccaaaaaa caacaattgc
     7741 acatgcactg cgttcggcaa aagacgcaga aattcaagcc ttttggtctg gcatcatctt
     7801 aaaatatcat cagtatcatc attgccatta taaatacata agaaaatgag aatacattaa
     7861 aggtatatac atacaatctt tgtcatagtc aacatatttt gcaacaaaat tatctataga
     7921 taacatgttt ttgtagaaaa ttccactaac tgattttcgt ataaatacaa attattctca
     7981 ttcttcaaaa tactcgccga cgtcatcgcg aaattcctca gcattcttcg acaaatgaag
     8041 agagacagcg agtgacacaa tcttatcttg ccgacgagtc tttgaaaatg gccccgggta
     8101 acggtgaaaa acatatagcc aagaatggag cgatcgccag gcgatggaaa ttggcttcaa
     8161 attgctccga atatggccag ttggtcagtt ggcattggat tgtggagaga tactagatca
     8221 gctcgttctc cttctcctcc gcattctcct actccgcccg aagacgcttc tttgatcgct
     8281 tctttctcga atgtgtacat attcattatg tatttggtgt ttggcgacga tcagccagct
     8341 ttaatggcca taaaacgtgt cttggtttgt gggccacata acaggcagcg agatctggaa
     8401 aaatgatgtg acagtggaaa tgagaagtag gtgcatctgc aaagatttga ttcagagttg
     8461 atgccattca tgattttttt ttagaagtat catttgtgtt tttagaatat agaattgcgg
     8521 ccgcaagctt ggatccgcta gcataacttc gtatagcata cattatacga agttatgcta
     8581 gcctagttcc agtgaaatcc aagcattttc taaattaaat gtattcttat tattatagtt
     8641 gttatttttg atatatataa acaacactat tatgcccacc atttttttga gatgcatcta
     8701 cacaaggaac aaacactgga tgtcactttc agttcaaatt gtaacgctaa tcactccgaa
     8761 caggtcacaa aaaattacct taaaaagtca taatattaaa ttagaataaa tatagctgtg
     8821 agggaaatat atacaaatat attggagcaa ataaattgta catacaaata tttattacta
     8881 atttctattg agacgaaatg aaccactcgg aaccatttga gcgaaccgaa tcgcgcggaa
     8941 ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg
     9001 ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc
     9061 gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg tgttttatgt agcgataaac
     9121 gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg gttagaagac aaatccaaag
     9181 tctacttgtg gggatgttcg aaggggaaat acttgtattc tataggtcat atcttgtttt
     9241 tattggcaca aatataatta cattagcttt ttgagggggc aataaacagt aaacacgatg
     9301 gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg ctactccttg
     9361 cgtcgggccc gaagtcttag agccagatat gcgagcaccc ggaagctcac gatgagaatg
     9421 gccagaccca cgtagtccag cggcagatcg gcggcggaga agttaagcgt ctccaggatg
     9481 accttgcccg aactggggca cgtggtgttc gacgatgtgc agctaatttc gcccggctcc
     9541 acgtccgccc attggttaat cagcagaccc tcgttggcgt aacggaacca tgagaggtac
     9601 gacaaccatt tgaggtatac tggcaccgag cccgagttca agaagaagcc gccaaagagc
     9661 aggaatggta tgataaccgg cggacccaca gacagcgcca tcgaggtcga ggagctggcg
     9721 caggatatta gatatccgaa ggacgttgac acattggcca ccagagtgac cagcgccagg
     9781 cagttgaaga agtgcagcac tccggcccgc agtccgatca tcggataggc aatcgccgtg
     9841 aagaccagtg gcactgtgag aaaaagcggc aattcggcaa tcgttttgcc cagaaagtat
     9901 gtgtcacagc gataaagtcg acttcgggcc tccctcataa aaactggcag ctctgaggtg
     9961 aacacctaaa tcgaatcgat tcattagaaa gttagtaaat tattgaaatg caaatgtatt
    10021 ctaaacatga cttacattta tcgtggcaaa gacgttttga aaggtcatgt tggtcaggaa
    10081 gaggaagatg gctccgttga tattcatcac acccacttgc gtgagttgtt ggcccaaaaa
    10141 gatgaggcca atcaagatgg caaccatctg caaattaaaa tgttactcgc atctcattaa
    10201 tattcgcgag ttaaatgaaa tttatttatc ttctgcaaaa ctataaacta tacatctcat
    10261 tgaaaaaaac taagaagggt gtggaatcag gcaattctat ctaaaatcta gcgaatttgt
    10321 ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac cgttgtctga
    10381 ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga tcgccacagg
    10441 accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc cggctgctcc
    10501 agtggcttct ccagattttt ggtggccaac aactgctcca tatcccgggc tactttgcta
    10561 atggcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc ccgtccgggc
    10621 acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg acactgggca
    10681 cccacgctgg ataggagttg agatgttatg taatactaga tacccttaat aaacacatcg
    10741 aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag ctaccctgcc
    10801 ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg gctgatgaat
    10861 ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga cgacgctgtg
    10921 ggcggtaaag gagtccagtc cggaggtggg ctcatcgcag atcagaagcg gcggatcggt
    10981 tagagcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac ctttcaccct
    11041 gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct ggatcacctg
    11101 atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca ccatggcttg
    11161 gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct gctggacata
    11221 ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca gtcgcatccc
    11281 ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca gcagggtcgt
    11341 ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg ccacgccgca
    11401 aactgagttt caaattggta attggaccct ttattaagat ttcacacaga tcagccgact
    11461 gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat gtgtcgctcg
    11521 ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc cggctgattg
    11581 accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc tagttggccg
    11641 ctccctgaac cggagtcctc cggcggactg ggtggccgga gcgtgccgta gtttttggcc
    11701 tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc ctgcaatgat
    11761 aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac aaaaagtacc
    11821 ccgaagtatc ctacgaagta ggtttatact tttatttatt ttttgtgcat ctaggatcag
    11881 cttaaaatat ctggttgtta tattttttgt aaaaaagaat atagtcgaaa atgaatgcct
    11941 ttagatgtct tgatcatgat atgatctcaa aaattgtctt atatagcgag aacagctacc
    12001 agaataatct gtttcgtgtc actatttgtt tgtgcaattg cggtttggga tttttgtggg
    12061 tcgcagttct cacgccgcag acaatttgat gttgcaatcg cagttcctat agatcaagtg
    12121 aacttaagat gtatgcacat gtactactca cattgttcag atgctcggca gatgggtgtt
    12181 tgctgcctcc gcgaattaat agctcctgat cctcttggcc cattgccggg atttttcaca
    12241 ctttcccctg cttacccacc caaaaccaat caccacccca atcactcaaa aaacaaacaa
    12301 aaataagaag cgagaggagt tttggcacag cactttgtgt ttaattgatg gcgtaaaccg
    12361 cttggagctt cgtcacgaaa ccgctgacaa aatgcaactg aaggcggaca ttgacgctac
    12421 gtaacgctac aaacggtggc gaaagagata gcggacgcag cggcgaaaga gacggcgata
    12481 tttctgtgga cagagaagga ggcaaacagc gctgactttg agtggaatgt cattttgagt
    12541 gagaggtaat cgaaagaacc tggtacatca aatacccttg gatcgaagta aatttaaaac
    12601 tgatcagata agttcaatga tatccagtgc agtaaaaaaa aaaaatgttt tttttatcta
    12661 ctttccgcaa aaatgggttt tattaactta catacatact aggggcccgg tacgtaccca
    12721 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
    12781 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
    12841 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
    12901 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    12961 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    13021 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    13081 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    13141 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    13201 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    13261 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    13321 aa
//
