LOCUS       sequence-458643-       16493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific expression of GFP in wing and haltere imaginal 
            discs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16493)
  AUTHORS   Port F, Buhmann MA, Zhou J, Stricker M, Vaughan-Brown A, Michalsen 
            A-C, Roßmanith E, Poltl A, Großkurth L, Huber J, Menendez Kury LB, 
            Weberbauer B, Hubl M, Heigwer F, Boutros M
  TITLE     Enhanced in vivo gene knockout with undetectable off-targets using 
            multiplexed Cas12a sgRNAs
  JOURNAL   bioRxiv 2024.11.26.625385
REFERENCE   2  (bases 1 to 16493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.26.625385"
COMMENT     Sequence Label: GMR11F02-Gal4 UAS-GFP
FEATURES             Location/Qualifiers
     source          1..16493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1957..1981
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5807..5928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5818..5837)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5872..5891
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6200..6220)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6350..6415
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    6506..6530
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(6531..7244,7245..7247,7248..7250))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6576..6597)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6925..6944
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7184..7205
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(7251..7275)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(7318..7556)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(7350..7371)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    7575..7669
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             complement(join(8477..9091,9162..9293,9497..9812,
                     9874..10528,10603..10876,11278..11349))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     11801..11819
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     complement(11852..11871)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11853..11871)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11878..11895)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11878..11894)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11887..11909)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12036..12491
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12123..12142)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12333..12354
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12517..12621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12622..12690,12691..13482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12840..12859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13653..14241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14142..14161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14395..14412
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14529..14550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14565..14570,14571..14588,14589..14595)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14603..14619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14608..14630
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14627..14643
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14627..14643
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        14664..14682
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    14901..14970
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     15028..15044
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15028..15044
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(15540..16493,1..1692))
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 atagctactt acagacataa cagttctttg tgctgcatcg cttaacatga tggagcatcg
       61 tttaacttca taactttgat gatcattttg atcctgttct agttgtgact ttttctgggt
      121 aaaattagtg aaaaaatctc ttaatacata aatgataaga gacaactgtt tccacttcag
      181 ttcgaatctt gtaaaggata gccaagggtg ttccttcaac aaattggtta gagcggtggt
      241 ggaaatatcc atttgtaaaa actttggtgc ctgtctcgaa acctcctcaa tctcattaca
      301 aatcatcaag catttttttg cacatatagg acttttttct gcagttactg ttttgtctag
      361 ttcatagatt tttgtgaaaa cttgtaagag ccttgctgtt tcaatgatgc catgatatat
      421 ggtgggacct gttgtggtac gctgcacatc gtcgacagaa gaagggaagg agattgtatt
      481 ctgagaaagc tggatggatc gaccataaag cagggacaat tggatctccc aagagtagac
      541 agaccaccaa attcggcgtc tttgttccag aatgctgcta tcactgaagg acgaggggag
      601 gtccctattc aagcccaatg atatggccat tcttatggaa aagctgtgaa aattatagct
      661 agtatttgtt ttctgcctcc actgtgtata tcgcgacaga agatgtaggg ctgtcaccaa
      721 aattatggaa cctgactcga agaccttgct cgtcaaatga gatttagcat tttgatagta
      781 aaaaacatct atatcagtag attccccctc tatacaccag gctccaatgg ctaatatgca
      841 gttaaaaagg atttgccatt gatccttcga cgcgatttca atctggttat tatacaacat
      901 cattagcgtc ggtgagtgca cgatagggca gtaggggtga aaattattga gataactttg
      961 aagtaaacgg gatgttgtgg atctagaagc caacgtgtat ctatccgtaa tcatggtcgg
     1021 gagcctgtta acgttagagt tcgtgtaatt ttccggttta aagccaatag atcgaagaat
     1081 acataagaga gaaccgtcgc caaagaaccc attattgttg gggtccgttt tcaggaaggg
     1141 caagccatcc gacatgtcat cctcttcaga ccaatcaaat ccatgaagag catccctggg
     1201 cataaaatcc aacggaattg tggagttatc atgatgagct gccgagtcaa tcgatacagt
     1261 caactgtctt tgacctttgt tactactctc ttccgatgat gatgtcgcac ttattctatg
     1321 ctgtctcaat gttagaggca tatcagtctc cactgaagcc aatctatctg tgacggcatc
     1381 tttattcaca ttatcttgta caaataatcc tgttaacaat gcttttatat cctgtaaaga
     1441 atccattttc aaaatcatgt caaggtcttc tcgaggaaaa atcagtagaa atagctgttc
     1501 cagtctttct agccttgatt ccacttctgt cagatgtgcc ctagtcagcg gagacctttt
     1561 ggttttggga gagtagcgac actcccagtt gttcttcaga cacttggcgc acttcggttt
     1621 ttctttggag cacttgagct ttttaagtcg gcaaatatcg catgcttgtt cgatagaaga
     1681 cagtagcttc atctttcagg aggcttgctt caagcttggg ctgcagattg tttagcttgt
     1741 tcagctgcgc ttgtttattt gcttacggga tcccggatct ggtaccgttt ggtatgcgtc
     1801 ttgtgattca aagttggctt attcaaagga tattaacgaa ggcagcggca cgtctgctcg
     1861 gctcgaacat tcattcacaa ctgatgctct cagccacccc gcgccctttt ataccgctgc
     1921 gctcgatccc cgggcgagct cggccggccg tttatcacca ctttgtacaa gaaagctggg
     1981 tcgaattcgc ccttggggat tggtccgaaa agaggataca actgaaaaat ggaaatgtga
     2041 aaaattcagt tgaaataaaa ctatttcccc gctatcaact agagttttcg agtccatata
     2101 ccaccttccc taccgtcctt cagcattcgt aaattattca caatatattg tcggttccag
     2161 taaagcaatt ttcaacaaat tttcacagca ctctgcgtga ctctaagtaa cggaaatatc
     2221 cgctaactat cacgggaaaa tcgagaagga acaagtttac caatacgacc accctctgat
     2281 cacgacagct gacggctttt ggtttcgatt cattgaaaca atatgaataa ttggccatag
     2341 ccaataagac tagttactgg aacaccagaa attgattgac agctgtggtg tgctagctgt
     2401 tgttgtcatt tcaaacgtca acacgtaccc ataatgacca ccaaaaaccc cattgaactc
     2461 gttggccacc agacggaact tggctggcaa attaatttct atcgagtgga aatatgttta
     2521 tgaattatta ctcaattgcc gtgggttcta cacctgatcg attacgtctg ttgtcatcgg
     2581 ttaggggact tgcattgcgg ccagggggtg ttccgttcaa aaaacaataa atattatgac
     2641 atattgcatg ttttgtatat aatttctgtg gtcagcagga ggcgagtcca tatacaagag
     2701 gtccgttgca tgattaaccc atatctaccc aatgcaggtc cttagtgcag ccaataaaaa
     2761 ctaaaaaaaa tatactatat aaagtcttta tttcataaag ctcataaagg ttcctttaaa
     2821 ttgtataaat ttgtattgct tcatttttag tataacataa tatatattcg catttttagt
     2881 atttatgatt ccgtcccacc tcgttcataa tgggttaatc ataaccacat ttccagttct
     2941 tagccaggga ttagccaaat gccaattggt aattcgtaaa ccagttcggg gcgccacttt
     3001 atccaattga aatgccaaac gactgccacg ccccccggca tccaggcggc cctcccttta
     3061 cccaaccgcc cccctttcgc catcgccggc gacggactcc tctgtcttat tcgcaatacg
     3121 aaattaattc tttgcatatt ggcatggtat tgtataaatt aaatttgctt acactgtcca
     3181 cgccccttgc atgttgttta gcatatgcca cgcccccaat tggggcttgc agcacttggc
     3241 atccttttcg gtcgcttaat tatagcttat cacatttcca tagccgcgga tcgagctcgt
     3301 ttttcatctt tgtcattctg tggggctctg taattgcggg tcttcgatat tttcacagtc
     3361 gcataaatca aaacggggag ttaacagcct ttgactcttc gaaacccctg caccctgcac
     3421 tcctcttcgc ctgaccttgg attttattat aataaaatcg caagttaggc attcggtata
     3481 ccgttagtcg ctgtaggcca caaaaactaa ttgcaaaaca aaagcattcg ccaaaaatgt
     3541 cacaacaaag caaagttccc gaaaaaaaag gagaaaactt tcggaaggcg cacaaaaaat
     3601 gtcgcagaca taggggaatg cgaagttttt tccgcccctc cctcattgtt tgctatttaa
     3661 attttgatgg cattgtgaac accaacaacg atcggtcttc aaagcggtgc cgaaagtctt
     3721 ttcgttggcc agaagggcgg ggtcatcgaa atccggtgcg gaatacagta gctatagaat
     3781 acagccagca ttagcatcat cgatctgcct ttgatgtatc tttgtatctg tctttggcat
     3841 ttcgtcatcg tcgatggctc gcaagctgct agcggtagtt ttcagggttg ccacatacat
     3901 aatttgtcat ttaaaacaaa aacttttgcc cagaatcaat aaaaaatttg gatttccaat
     3961 aaaattccta tttattataa caattattgt cacaaaaaca gaattttatt ttctttaaat
     4021 taaaatcaat ataatgaaaa ataagtaatt gtcaagctta cttcattgac ttggcaactc
     4081 tggtcgttgt ttttgtttgc cccagcagac agaatgttgg cgatttaaat tttttggcag
     4141 cggcaagaag cggctgcaac cgcaaagtcg aaagccaaac aacaatcggc aacaatcaac
     4201 taccaattca gtctataacc cgaaaaccga aagccgaaaa tatttatgca ttttgatgcg
     4261 gcaaaatgaa tgatttgact ttgacgaaaa gatgtcaatg ccatgaggag aagtcgcaaa
     4321 aagacgtaca cacacacaca cagcgcgccg aaagacaaaa gctggtcaca gaaggaatgc
     4381 attggggaca aggactggtc gcttttggtg gatattagtg caaaacgctt ggatcctgcc
     4441 gacaatttaa atgcgcgcat ggggcaggag gagcggcaga acctgatccg gtccacagtg
     4501 gcaacaataa tcagaacatg gcctcaactc aactcaactc aactcagctc agctcggctc
     4561 agctcaacgc cgctcctttg cttttgcttt gctttgtggc aaatgtcgtt gatgccgctt
     4621 gatgaaagcg tcagaaattg atggatgtga atgctggctg aatgctgtga gaggggttct
     4681 ggccagggac tttggctccc agccgcaacg caaatctttt gggctactgt tgggagaccc
     4741 ggagctcttg tccctggccc catccatcgg ctttgtggcc tttgaagtcg ctcgttgcgg
     4801 tagcaatttg tgctttaatt tgagtttaaa aactatgcac agctccagct gaagttccat
     4861 tgttaaagcg tagcgaaaat tgacggacaa agctgcgtct tctacaatat atgggtatgt
     4921 gtcatttgtg gccctaaaaa gtcaacaaat tagtaaattt catctaatag acaaacgaca
     4981 aaaaagcatt ccatattatt tgtatcattt aaatatcaat gcgatatacg tttcattttc
     5041 catctgcatg tccagttgac aaggaataca ccaaagaaat accataaaaa tgccgaacac
     5101 aaggcagaga catttaaatt tgttgtaata aattgtgcga tgtacgagcg gccatcgaaa
     5161 ataacaaccg cgaagacaaa gcgtaaccga ccatcaatcg cgatctgaag tgcgacaggg
     5221 aacgggagac attgcggcag aaatgattgc agttcataag gccgactgcc ttctgaatat
     5281 atgcgtgtaa tggcttcctt aaggcccaaa ccaaagccaa ccaaaacggg taggaggagg
     5341 gggcaagagg agggcagggg tacgatcttc agcacatgat caaaaatcat acggccgaca
     5401 gtggaagtca agaaaggcaa gacccgctca ccgatggcca acatcatgat gataatgatg
     5461 aggttgtcaa cgtggccaat gccgagaatc agtcaccagc aaaattaacg gacagtgtca
     5521 aggatggagt ttcgattatt cgtagttcct gactaaatat ggtgtttata ctaaacggat
     5581 ttagtaatta ttattattat cagaataatg atagtaccta atgtgtgtat tgtactgatg
     5641 tagaaactta tcttattgca atatcaagat tctttaggga agaaaaagtt tctagtcttt
     5701 aaaatattag taatatgttt tacttaattt ccaattattt atttaagtac tttatgtttt
     5761 tattaaaagg tgcagccagc agttacaagt tgaaggagag acatgataag atacattgat
     5821 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     5881 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     5941 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     6001 aacctctaca aatgtggtat ggctgattat gatctctagt caaggcacta tacatcaaat
     6061 atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg taaatagcaa
     6121 agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc aattctgaag
     6181 gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa tgttgagagt
     6241 cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg ttttcctcat
     6301 taaaggcatt ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac
     6361 aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata tttaccttag
     6421 agctttaaat ctctgtaggt agtttgtcca attatgtcac accacagaag taaggttcct
     6481 tcacaaagat ccgctagctt acgtcaccac tttgtacaag aaagctgggt ttacttgtac
     6541 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     6601 tcgcgcttct cgttggggtc tttgctcagc ttggactggg tgctcaggta gtggttgtcg
     6661 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     6721 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     6781 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     6841 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     6901 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     6961 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     7021 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     7081 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     7141 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     7201 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat agcctgcttt
     7261 tttgtacaaa cttgtatacc ggtagcagat ctgttaacga attcccaatt ccctattcag
     7321 agttctcttc ttgtattcaa taattacttc ttggcagatt tcagtagttg cagttgattt
     7381 acttggttgc tggttacttt taattgattc actttaactt gcactttact gcagattgtt
     7441 tagcttgttc agctgcgctt gtttatttgc ttagctttcg cttagcgacg tgttcacttt
     7501 gcttgtttga attgaattgt cgctccgtag acgaagcgcc tctatttata ctccggcgct
     7561 cgctagagtc tccgctcgga ggacagtact ccgctcggag gacagtactc cgctcggagg
     7621 acagtactcc gctcggagga cagtactccg ctcggaggac agtactccga cctgcaggca
     7681 tgcaagcatt taaatcctaa gctctagcct agttccagtg aaatccaagc attttctaaa
     7741 ttaaatgtat tcttattatt atagttgtta tttttgatat atataaacaa cactattatg
     7801 cccaccattt ttttgagatg catctacaca aggaacaaac actggatgtc actttcagtt
     7861 caaattgtaa cgctaatcac tccgaacagg tcacaaaaaa ttaccttaaa aagtcataat
     7921 attaaattag aataaatata gctgtgaggg aaatatatac aaatatattg gagcaaataa
     7981 attgtacata caaatattta ttactaattt ctattgagac gaaatgaacc actcggaacc
     8041 atttgagcga accgaatcgc gcggaactaa cgacagtcgc tccaaggtcg tcgaacaaaa
     8101 ggtgaatgtg ttgcggagag cgggtgggag acagcgaaag agcaactacg aaacgtggtg
     8161 tggtggaggt gaattatgaa gagggcgcgc gatttgaaaa gtatgtatat aaaaaatata
     8221 tcccggtgtt ttatgtagcg ataaacgagt ttttgatgta aggtatgcag gtgtgtaagt
     8281 cttttggtta gaagacaaat ccaaagtcta cttgtgggga tgttcgaagg ggaaatactt
     8341 gtattctata ggtcatatct tgtttttatt ggcacaaata taattacatt agctttttga
     8401 gggggcaata aacagtaaac acgatggtaa taatggtaaa aaaaaaaaac aagcagttat
     8461 ttcggatata tgtcggctac tccttgcgtc gggcccgaag tcttagagcc agatatgcga
     8521 gcacccggaa gctcacgatg agaatggcca gacccacgta gtccagcggc agatcggcgg
     8581 cggagaagtt aagcgtctcc aggatgacct tgcccgaact ggggcacgtg gtgttcgacg
     8641 atgtgcagct aatttcgccc ggctccacgt ccgcccattg gttaatcagc agaccctcgt
     8701 tggcgtaacg gaaccatgag aggtacgaca accatttgag gtatactggc accgagcccg
     8761 agttcaagaa gaagccgcca aagagcagga atggtatgat aaccggcgga cccacagaca
     8821 gcgccatcga ggtcgaggag ctggcgcagg atattagata tccgaaggac gttgacacat
     8881 tggccaccag agtgaccagc gccaggcagt tgaagaagtg cagcactccg gcccgcagtc
     8941 cgatcatcgg ataggcaatc gccgtgaaga ccagtggcac tgtgagaaaa agcggcaatt
     9001 cggcaatcgt tttgcccaga aagtatgtgt cacagcgata aagtcgactt cgggcctccc
     9061 tcataaaaac tggcagctct gaggtgaaca cctaaatcga atcgattcat tagaaagtta
     9121 gtaaattatt gaaatgcaaa tgtattctaa acatgactta catttatcgt ggcaaagacg
     9181 ttttgaaagg tcatgttggt caggaagagg aagatggctc cgttgatatt catcacaccc
     9241 acttgcgtga gttgttggcc caaaaagatg aggccaatca agatggcaac catctgcaaa
     9301 ttaaaatgtt actcgcatct cattaatatt cgcgagttaa atgaaattta tttatcttct
     9361 gcaaaactat aaactataca tctcattgaa aaaaactaag aagggtgtgg aatcaggcaa
     9421 ttctatctaa aatctagcga atttgtttcc aagaattgta agcgttatat catttgtttc
     9481 cactggaacc actcaccgtt gtctgaataa gtcgcacttt tacgaggagt ggttccttga
     9541 gcaccgacag ccaggatcgc cacaggaccg cccggaactg catgaaccag gtggccttgt
     9601 aggtgtaccc attctccggc tgctccagtg gcttctccag atttttggtg gccaacaact
     9661 gctccatatc ccgggctact ttgctaatgg caaaattgtc gcatatcttg gcgatccgat
     9721 cacgggactc gatctcccgt ccgggcacaa cggccaacac ctgtacgtaa aagtccgccg
     9781 gattgtagtt ggtaggacac tgggcaccca cgctggatag gagttgagat gttatgtaat
     9841 actagatacc cttaataaac acatcgaact cactaggaaa agaagtcgac ggcttcgctg
     9901 ggagtgccca agaaagctac cctgccctcg gccatcagaa ggatcttgtc aaagagctca
     9961 aacagctcgg aagacggctg atgaatggtc aggatgacgg tcttgccctt ctgcgacagc
    10021 ttcttcagca cctggacgac gctgtgggcg gtaaaggagt ccagtccgga ggtgggctca
    10081 tcgcagatca gaagcggcgg atcggttaga gcctcggagg cgaatgccag acgcttcctt
    10141 tctccgccgg acagaccttt caccctgccg ggcacaccga tgatcgtgtg ctgacatttg
    10201 ctgagcgaaa gctcctggat cacctgatcc acgcgggcca ctcgctgccg ataggtcaga
    10261 tgtcgtggca tccgcaccat ggcttggaaa atcaggtgtt ccctggccgt tagggagccg
    10321 ataaagaggt catcctgctg gacataggcg cacctggcct gcatctcctt ggcgtccaca
    10381 ggttggccat tgagcagtcg catcccggat ggcgatactt ggatgccctg cggcgatcga
    10441 aaggcaaggg cattcagcag ggtcgtcttt ccggcaccgg aactgcccat cacggccaaa
    10501 agttcgcccg gataggccac gccgcaaact gagtttcaaa ttggtaattg gaccctttat
    10561 taagatttca cacagatcag ccgactgcga atagaaactc accgttcttg agcaaatgtt
    10621 tcctgggcgc cggtatgtgt cgctcgttgc agaatagtcc gcgtgtccgg ttgaccagct
    10681 gccgccatcc ggagcccggc tgattgaccg ccccaaagat gtccatattg tgccaggcat
    10741 aggtgaggtt ctcggctagt tggccgctcc ctgaaccgga gtcctccggc ggactgggtg
    10801 gccggagcgt gccgtagttt ttggcctgcc cgaagccctg gttaatgcag ctctgcgaag
    10861 ccgctccgct gtcaccctgc aatgataggg gatctcaaat atcaactaca agcgttatgc
    10921 tcatctaacc ccgaacaaaa agtaccccga agtatcctac gaagtaggtt tatactttta
    10981 tttatttttt gtgcatctag gatcagctta aaatatctgg ttgttatatt ttttgtaaaa
    11041 aagaatatag tcgaaaatga atgcctttag atgtcttgat catgatatga tctcaaaaat
    11101 tgtcttatat agcgagaaca gctaccagaa taatctgttt cgtgtcacta tttgtttgtg
    11161 caattgcggt ttgggatttt tgtgggtcgc agttctcacg ccgcagacaa tttgatgttg
    11221 caatcgcagt tcctatagat caagtgaact taagatgtat gcacatgtac tactcacatt
    11281 gttcagatgc tcggcagatg ggtgtttgct gcctccgcga attaatagct cctgatcctc
    11341 ttggcccatt gccgggattt ttcacacttt cccctgctta cccacccaaa accaatcacc
    11401 accccaatca ctcaaaaaac aaacaaaaat aagaagcgag aggagttttg gcacagcact
    11461 ttgtgtttaa ttgatggcgt aaaccgcttg gagcttcgtc acgaaaccgc tgacaaaatg
    11521 caactgaagg cggacattga cgctacgtaa cgctacaaac ggtggcgaaa gagatagcgg
    11581 acgcagcggc gaaagagacg gcgatatttc tgtggacaga gaaggaggca aacagcgctg
    11641 actttgagtg gaatgtcatt ttgagtgaga ggtaatcgaa agaacctggt acatcaaata
    11701 cccttggatc gaagtaaatt taaaactgat cagataagtt caatgatatc cagtgcagta
    11761 aaaaaaaaaa atgttttttt tatctacttt ccgcaaaaat gggttttatt aacttacata
    11821 catactaggg gcccggtacg tacccaattc gccctatagt gagtcgtatt acaattcact
    11881 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
    11941 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
    12001 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
    12061 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
    12121 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    12181 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    12241 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    12301 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    12361 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    12421 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
    12481 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    12541 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    12601 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    12661 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    12721 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    12781 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    12841 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    12901 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    12961 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    13021 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    13081 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    13141 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    13201 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    13261 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    13321 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    13381 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    13441 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    13501 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    13561 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    13621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    13681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    13741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    13801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    13861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    13921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    13981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    14041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    14101 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    14161 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    14221 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    14281 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    14341 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    14401 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    14461 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    14521 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    14581 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    14641 gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggctaga
    14701 actagtgtcg acatgcccgc cgtgaccgtc gagaacccgc tgacgctgcc ccgcgtatcc
    14761 gcacccgccg acgccgtcgc acgtcccgtg ctcaccgtga ccaccgcgcc cagcggtttc
    14821 gagggcgagg gcttcccggt gcgccgcgcg ttcgccggga tcaactaccg ccacctcgac
    14881 ccgttcatca tgatggacca gatgggtgag gtggagtacg cgcccgggga gcccaagggc
    14941 acgccctggc acccgcaccg cggcttcgag accgtgacct acatcgtcga cactagtgga
    15001 tctaagctta ccgagctcag cggccgctct agaactagtg gatctaaacg agtttttaag
    15061 caaactcact ccctgacaat aaaaacgcaa ttatctttaa ataaatatat tctatttatt
    15121 aaccaagtag ctaatatagc taaaatcaat ttgttgctaa cttaaaacta acataattat
    15181 gtattagtta tttacattct tttggttggt tacaaccaaa taaataatat cacttttaaa
    15241 aacttaagcc aggaactgaa taattttcta tttggcttta gtcgacggta tcgataagct
    15301 tctgaataag ccctcgtaat atattttcat gaagaattta ggtccaaaaa aaagatgggc
    15361 attaattcta gtcatttaaa aaattctata gatcagaggt tacatggcca agattgaaac
    15421 ttagaggagt atagttacat aaaagaaggc aaaacgatgt ataaatgaaa gaaattgaga
    15481 tggtgcacga tgcacagttg aagtgaactt gcggggtttt tcagtatcta cgattcattt
    15541 tactcttttt ttgggtttgg tggggtatct tcatcatcga atagatagtt atatacatca
    15601 tccattgtag tggtattaaa catccctgta gtgattccaa acgcgttata cgcagtttgg
    15661 tccgtccaac caggtgacag tggttttgaa ttattaccat catcaatttt actagccgtg
    15721 atttcattat tcatgaagtt atcatgaacg ttagaggagg caattggttg tgaaagcgct
    15781 tgagaatttg tttgagttgt tatgaggttc ggaccgttgc tactgttagt gaaagtgaag
    15841 gacaatgagc tatcagcaat attcccactt tgattaaaat tggcgccacc aaacaaagca
    15901 gacggggtca gtggcactaa tgattgcagc tgttgctgtt gccctagaaa aggcgtgact
    15961 gagcgatgcg aaggtgtgct tcttggtatt gtcactggag agttacgaga gggtggacgg
    16021 ttagataaca gcttgactag atcactgaaa cttgctcctg atttcaatgg cacaggtgaa
    16081 ggccctactg agccaggaga aacatattta acactgatat tgttgacatt ttcctccgga
    16141 agagtagggt attgggcgat agttgcagaa ccgacaatat ttttaatggc gctaccatta
    16201 ctattgttat aactgatatg cggtaatggg attgcacact gtgataacag aaacggcgca
    16261 catacctctt ccagtacttg aatgtatttt tcacaagtct ggattttaaa agtggccagt
    16321 ttttttaata gcatcagaac agtgttaatt tgttgtaata attgtgcggt ctcgttattc
    16381 tcagcattcg attttgagtt tgagagtaga gtctttatgg gtactaggac tgcattgaac
    16441 aagtaataag aacaattcca ggcaaaatat ggggtgacat tatgattgtc cat
//
