LOCUS       sequence-458645-       14201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitous expression of mScarlet in Drosophila melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14201)
  AUTHORS   Port F, Buhmann MA, Zhou J, Stricker M, Vaughan-Brown A, Michalsen 
            A-C, Roßmanith E, Poltl A, Großkurth L, Huber J, Menendez Kury LB, 
            Weberbauer B, Hubl M, Heigwer F, Boutros M
  TITLE     Enhanced in vivo gene knockout with undetectable off-targets using 
            multiplexed Cas12a sgRNAs
  JOURNAL   bioRxiv 2024.11.26.625385
REFERENCE   2  (bases 1 to 14201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.26.625385"
COMMENT     Sequence Label: act5C-mScarlet
FEATURES             Location/Qualifiers
     source          1..14201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(382..453,855..1128,1203..1857,1919..2234,2438..2569,
                     2640..3254)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4027..4060
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    10962..11031
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(11250..11268)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11289..11305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11289..11305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11302..11324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11313..11329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11337..11343,11344..11361,11362..11367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11382..11403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11520..11537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11691..12279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11771..11790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12450..13241,13242..13310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13073..13092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13311..13415)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13441..13896)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13578..13599)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13790..13809
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14023..14045
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14037..14054
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14038..14054
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14061..14080
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14061..14079
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14113..14131)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 atcagtttta aatttacttc gatccaaggg tatttgatgt accaggttct ttcgattacc
       61 tctcactcaa aatgacattc cactcaaagt cagcgctgtt tgcctccttc tctgtccaca
      121 gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg
      181 ttacgtagcg tcaatgtccg ccttcagttg cattttgtca gcggtttcgt gacgaagctc
      241 caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt
      301 atttttgttt gttttttgag tgattggggt ggtgattggt tttgggtggg taagcagggg
      361 aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc
      421 agcaaacacc catctgccga gcatctgaac aatgtgagta gtacatgtgc atacatctta
      481 agttcacttg atctatagga actgcgattg caacatcaaa ttgtctgcgg cgtgagaact
      541 gcgacccaca aaaatcccaa accgcaattg cacaaacaaa tagtgacacg aaacagatta
      601 ttctggtagc tgttctcgct atataagaca atttttgaga tcatatcatg atcaagacat
      661 ctaaaggcat tcattttcga ctatattctt ttttacaaaa aatataacaa ccagatattt
      721 taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc gtaggatact
      781 tcggggtact ttttgttcgg ggttagatga gcataacgct tgtagttgat atttgagatc
      841 ccctatcatt gcagggtgac agcggagcgg cttcgcagag ctgcattaac cagggcttcg
      901 ggcaggccaa aaactacggc acgctccggc cacccagtcc gccggaggac tccggttcag
      961 ggagcggcca actagccgag aacctcacct atgcctggca caatatggac atctttgggg
     1021 cggtcaatca gccgggctcc ggatggcggc agctggtcaa ccggacacgc ggactattct
     1081 gcaacgagcg acacataccg gcgcccagga aacatttgct caagaacggt gagtttctat
     1141 tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc caattaccaa tttgaaactc
     1201 agtttgcggc gtggcctatc cgggcgaact tttggccgtg atgggcagtt ccggtgccgg
     1261 aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc aagtatcgcc
     1321 atccgggatg cgactgctca atggccaacc tgtggacgcc aaggagatgc aggccaggtg
     1381 cgcctatgtc cagcaggatg acctctttat cggctcccta acggccaggg aacacctgat
     1441 tttccaagcc atggtgcgga tgccacgaca tctgacctat cggcagcgag tggcccgcgt
     1501 ggatcaggtg atccaggagc tttcgctcag caaatgtcag cacacgatca tcggtgtgcc
     1561 cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg cctccgaggc
     1621 tctaaccgat ccgccgcttc tgatctgcga tgagcccacc tccggactgg actcctttac
     1681 cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga ccgtcatcct
     1741 gaccattcat cagccgtcat ccgagctgtt tgagctcttt gacaagatcc ttctgatggc
     1801 cgagggcagg gtagctttct tgggcactcc cagcgaagcc gtcgacttct tttcctagtg
     1861 agttcgatgt gtttattaag ggtatctagt attacataac atctcaactc ctatccagcg
     1921 tgggtgccca gtgtcctacc aactacaatc cggcggactt ttacgtacag gtgttggccg
     1981 ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc caagatatgc gacaattttg
     2041 ccattagcaa agtagcccgg gatatggagc agttgttggc caccaaaaat ctggagaagc
     2101 cactggagca gccggagaat gggtacacct acaaggccac ctggttcatg cagttccggg
     2161 cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac
     2221 ttattcagac aacggtgagt ggttccagtg gaaacaaatg atataacgct tacaattctt
     2281 ggaaacaaat tcgctagatt ttagatagaa ttgcctgatt ccacaccctt cttagttttt
     2341 ttcaatgaga tgtatagttt atagttttgc agaagataaa taaatttcat ttaactcgcg
     2401 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     2461 catctttttg ggccaacaac tcacgcaagt gggtgtgatg aatatcaacg gagccatctt
     2521 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcatgt
     2581 ttagaataca tttgcatttc aataatttac taactttcta atgaatcgat tcgatttagg
     2641 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     2701 acacatactt tctgggcaaa acgattgccg aattgccgct ttttctcaca gtgccactgg
     2761 ttttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     2821 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     2881 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     2941 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3001 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3061 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3121 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3181 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     3241 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt ttaccattat
     3301 taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca
     3361 ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt
     3421 agactttgga tttgtttcta accaaaagac ttacacacct gcatacctta catcaaaaac
     3481 tcgtttatcg ctacataaaa caccgggata tattttttat atacatactt ttcaaatcgc
     3541 gcgccctctt cataattcac ctccaccaca ccacgtttcg tagttgctct ttcgctgtct
     3601 cccacccgct ctccgcaaca cattcacctt ttgttcgacg accttggagc gactgtcgtt
     3661 agttccgcgc gattcggttc gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa
     3721 attagtaata aatatttgta tgtacaattt atttgctcca atatatttgt atatatttcc
     3781 ctcacagcta tatttattct aatttaatat tatgactttt taaggtaatt ttttgtgacc
     3841 tgttcggagt gattagcgtt acaatttgaa ctgaaagtga catccagtgt ttgttccttg
     3901 tgtagatgca tctcaaaaaa atggtgggca taatagtgtt gtttatatat atcaaaaata
     3961 acaactataa taataagaat acatttaatt tagaaaatgc ttggatttca ctggaactag
     4021 gctagcataa cttcgtataa tgtatgctat acgaagttat gctagcggat tcgaagacag
     4081 atggagatct cgctgcctgt tatgtggccc acaaaccaag acacgtttta tggccattaa
     4141 agctggctga tcgtcgccaa acaccaaata cataatgaat atgtacacat tcgagaaaga
     4201 agcgatcaaa gaagcgtctt cgggcggagt aggagaatgc ggaggagaag gagaacgagc
     4261 tgatctagta tctctccaca atccaatgcc aactgaccaa ctggccatat tcggagcaat
     4321 ttgaagccaa tttccatcgc ctggcgatcg ctccattctt ggctatatgt ttttcaccgt
     4381 tacccggggc cattttcaaa gactcgtcgg caagataaga ttgtgtcact cgctgtctct
     4441 cttcatttgt cgaagaatgc tgaggaattt cgcgatgacg tcggcgagta ttttgaagaa
     4501 tgagaataat ttgtatttat acgaaaatca gttagtggaa ttttctacaa aaacatgtta
     4561 tctatagata attttgttgc aaaatatgtt gactatgaca aagattgtat gtatatacct
     4621 ttaatgtatt ctcattttct tatgtattta taatggcaat gatgatactg atgatatttt
     4681 aagatgatgc cagaccaaaa ggcttgaatt tctgcgtctt ttgccgaacg cagtgcatgt
     4741 gcaattgttg ttttttggaa tattcaattt tcggactgtc cgctttgatt tcagtttctt
     4801 ggcttattca aaaagcaaag taaagccaaa aaagcgagat ggcaatacca aatgcggcaa
     4861 aacggtagtg gaaggaaagg ggtgcggggc agcggaagga agggtggggc ggggcgtggc
     4921 ggggtctgtg gctgggcgcg acgtcaccga cgttggagcc actcctttga ccatgtgtgc
     4981 gtgtgtgtat tattcgtgtc tcgccactcg ccggttgttt ttttcttttt atgctgcgct
     5041 ctctctagcg ccatctcgct tacgcatgct caacgcaccg catgttgccg tttcctttta
     5101 tgcgtcattt tggctcgaaa taggcaatta tttaaacaaa gattagtcaa cgaaaacgct
     5161 aaaataaata agtctacaat atggttactt attgccatgt gtgtgcagcc aacgatagca
     5221 acaaaagcaa caacacaggt ggctttccct ctttcacttt ttgtttgcaa gccgcgtgcg
     5281 agcaagacgg cacgaccggc aaacgcaatt acgctgacaa agagcagacg aagttttggc
     5341 gaaaaacatc aaggcgcctg atacgaatgc atttgcaata acaattgcga tatttaatat
     5401 tgtttatgaa gctgtttgac ttcaaaacac acaaaaaaaa aaaataaaac aaattatttg
     5461 aaagagaatt aggaatcgga cgcttatcgt tagggtaaca acaagaaatg cttactgagt
     5521 cacagcctct ggaaaactgc cgcaagccag agagagagag aaaaagaggg agagcagctt
     5581 agaccgcatg tgcttgtgtg tgaggcgtct ctctcttcgt ctctgttgcg caaacgcata
     5641 gactgcactg aaaaaatcga ttacctattt tttatgaatg aatatttgca ctattactat
     5701 tcaaaactat taagatagca atcacattca atagccaaat actataccac ctgagcgatg
     5761 caacgaaatg atcaatttga gcaaaaatgc tgcatattta ggacggcatc attatagaaa
     5821 tgcttcttgc tgtgtacttt tctctcgtct ggcagctgtt tcgccgttat tgttaaaacc
     5881 ggcttaagtt aggtgtgttt tctacgacta gtgaatgccc tactagaaga tgtgtgttgc
     5941 acaaaatgtc cctggaataa ccaatttgaa gtgcagatag cagtaaacgt aagctaatat
     6001 gaatattatt taactgtaat gttttaatat cgctggacat tactaataaa cccactataa
     6061 acacatgtac atatgtatgt tttggcatac aatgagtagt tggggaaaaa atgtgtaaaa
     6121 gcaccgtgac catcacagca taaagataac cagctgaagt atcgaatatg agtaaccccc
     6181 aaattgaatc acatgccgca actgatagga cccatggaag tacactcttc atggcgatat
     6241 acaagacaca cacaagcacg aacacccagt tgcggaggaa attctccgta aatgaaaacc
     6301 caatcggcga acaattcata cccatatatg gtaaaagttt tgaacgcgac ttgagagcgg
     6361 agagcattgc ggctgataag gttttagcgc taagcgggct ttataaaacg ggctgcggga
     6421 ccagttttca tatcactacc gtttgagttc ttgtgctgtg tggatactcc tcccgacaca
     6481 aagccgctcc atcagccagc agtcgtctaa tccagagaca ccaaaccgaa agacttaatt
     6541 tatatttatt taattaattt taataaaaca caccaaatgt aagtagcttt ccccttccca
     6601 acaacaaaac accatcgaac cactcccacc aagaaaaagc aataatcgag aaaagccgcg
     6661 gaaaatgtgt gatttttttt gtaaacaaaa cattttttta tgtgccagtg ctgaaagtga
     6721 tcaaaaaata ctagccacga gctaaagagt tattgtattg accaaaactc caaaaatacc
     6781 caagtttggc cctaaattgt caatcaaaat accaataggt cgaaagacat caaaattaac
     6841 aaaaccaggg tttcaaatac cataactcaa gaatcaggat tacaactgca gatttcagga
     6901 tatatacata caaattatac gaaattataa aaaccaaagc aattcaatag ccccaactca
     6961 aatgttagga tctaatatag tgtttaaagc caagctcgct gatgtgggcg tgtcacgatt
     7021 tcacccaaag atatgccaaa ttacgaattg caaatcaatt cgccaacact ttcttttttt
     7081 cccacgccct aaaacaccag atcatccata aatgtacata catacagtat atgcatatta
     7141 taatctgtaa aactagatca ggttcttgaa aatagtgacg taggcagccg ttttggctga
     7201 agcagaaatt tttgccggtt tttcaaagtt gtagttgcaa aaatggagaa aaccttcgag
     7261 cattcgttca tatacacaca ctcacgcgca aaataacgag agagagtgta tgtgtgtgtg
     7321 agagagcgaa agccagacga cggtttgctt ttcgcctcga aacatgacca tatatggtca
     7381 caaaacttgg ccgccgcaat tcaacacacc agcgctctcc ttcgcaccca tagcgaccat
     7441 ggcgcggagc gagcgagatg gcgagagcga gcgacgccta tggcgacgtc gacgcaggca
     7501 gcgattgaaa aacgcagtta actggcattc aacattcacc agccactttc agtcggttta
     7561 ttccagtcat tcctttcaaa ccgtgcggtc gcttagctca gcctcgccac ttgcgtttac
     7621 agtagttttc acgccttgaa tttgttaaat cgaacaaaaa ggtaaagttt aactagcttt
     7681 gaaaagtttc gtggctctta attgttaaat tttctagagt gcgtttagtg tttttttttt
     7741 tttttatttt gtaatgttaa tttcgggttc caattcgagt tttaggcagc cgccatttta
     7801 agggcgcata cacacaggca actgtgctct ctttgcggct ttcttttgca ccggcattcg
     7861 ttaagtgtcg tctagaagct tctcccctcc cttttcggca tattcgtatt gtggttttaa
     7921 tttttcgggg cggggccttc tattttgtaa ctgttctttt aatttcttat tacaattcga
     7981 tcgcaagtga aaatcagttt tcaatcggaa aagtattttt ttatgaaatt tttttttgtc
     8041 caagattaaa attttgtact aaaaaaacgt acattgcatt gagtgatttt taattgtaca
     8101 cgaaaaacaa gttagtttgt tatgacaatt gtactttggt agaccagcgc agtccaagga
     8161 aaccacgcaa attctcagtt ttttttttgc catttctaca ttaccaaata aggtaaccaa
     8221 aaactaatgg gaaatccgca ttctttccat tgcagcttac aggatcgatc cccggatggt
     8281 tagtaagggc gaagctgtaa taaaggagtt tatgaggttc aaggtacaca tggagggttc
     8341 gatgaatggc catgaatttg agatcgaagg agagggtgag ggacggcctt acgagggcac
     8401 gcaaactgcc aagttgaagg tgactaaagg cggccccttg cctttctcct gggatatctt
     8461 gtcgccacaa ttcatgtacg gatcgcgggc ctttacaaag catcctgcgg acataccgga
     8521 ctattacaag caatccttcc cggagggctt caaatgggaa cgggttatga actttgaaga
     8581 tggaggagcc gttacggtta ctcaagatac gagtctggag gacggaaccc tgatctacaa
     8641 ggtgaagttg cgtggaacaa attttcctcc agatggtccg gtcatgcaaa agaaaacgat
     8701 gggctgggag gcatcgacgg aacgcctgta ccccgaggat ggcgtgttga agggtgatat
     8761 aaaaatggcc ttgaggctca aagatggtgg taggtacctc gctgatttta aaacgacata
     8821 caaggcgaaa aagccagtcc agatgcccgg cgcgtataat gttgaccgta agctcgacat
     8881 aacgagccac aacgaggact acactgtggt tgaacaatac gagcggagcg agggtaggca
     8941 ttccactggc ggaatggatg aactgtataa gtgatctaga gaaggatcgc ttgtctgggc
     9001 aagaggatca ggatcgggat ggtcttgatt ctgctggagg aggaggagga gaagtcgagg
     9061 aagcagcagc gaaagtgcaa gtgcgagtgg tggaagtttg gagtgcagca caacaaaatc
     9121 aacaacaaca ccaactacaa gatgaaaaga gcggaaccac ctgcacacca tcatcactat
     9181 catcatcgtt ttgggcgcat gttgtgtggt tccagcgtat taatataatt aatttattcc
     9241 acatgagata tgatatgata tactatgtat tttttgtttt ttttttattt gtaaaccttt
     9301 aatataacaa gaactacaaa aaatgaaaat gagcgaaaat gcatattctg ccattccaca
     9361 cacacaccaa caacacccaa cacacgcaca cccacaagct tacacacaca cattcgcggc
     9421 atgacaagga catcaagata aagaagaact taaagaagat atttcccaaa gcgcaaaaag
     9481 aacacacaca cattgcaaaa cacaaacaac acactagcgt tttgtacaat tcgtcagcaa
     9541 ccttatgtat tattttttaa ttatgatgta attataaaca aagtgaaaca aaaatatgaa
     9601 aacaaaaagg aaaatcaaat ctgtcttctc tttctcccgc tctcctcgct ctctgctgct
     9661 aacctcgccc tctcctctct catctttttg tctgtctctc ttccacattt ttgccggccg
     9721 gcaaaataat aacccacaca cactcacact tggctgcagt ttcgcgtgcg atattcacac
     9781 acattcaagc atacaagcat acatacatat gtattttttt tttatttgta cacttttcta
     9841 attgcatgcg tatcgattga taagtttacg cctgaaaatg ttaattaaaa tgtgaaaatg
     9901 caactgaaaa actgatgaaa tgaaacaaca acaagcgaac aatttgctac atgtgtattg
     9961 tctaacaacc gttactgcaa cggttgcttc ggaaaagggt gaagaggaag agggcacgag
    10021 gtcgacgaac taaggctgcg acgacaagcg gctgtctcgc tcgcacgcac gcacgcatgc
    10081 gggctaatga tgcgattaaa gtgccgtttt aaatggccaa gatccaaata aaagtcgaga
    10141 taataaggca aacacgctaa cacaaatcgc tggcatagtt tcatccgcca gtttggccaa
    10201 aagcagcggc tgcaagcgcc gtttatccgg tttttcccaa acattttcca ttcatgcggc
    10261 caaagaggag gagcacccat ctttccatct ttctctgact cgcacgggca atgcagtgca
    10321 ccggaaagaa agtggtgcga attgggattt atagacttac tttatagact tacacgtaca
    10381 aatatacata gatattatat tatttaaata catttttatt atattgctta tatattagtt
    10441 cgacttcgat atcatttctg ccagtataat tatgggctct gcttgatttg gcgctcggat
    10501 gctacggtaa aagttatctt tctgtgcttt ctcctttgat tcttgagttt caacggttcc
    10561 gtttttgttg atctcgttca tcttcttctt cttgcgttct agacgttctc ctttgttttt
    10621 tgttcacaac ggatttcgta gcaccttgag cggtttaaat atacatacat atgtaataca
    10681 tacataaata catatgtagt tagtgttttt tttcgatatt cgtatgccca aatgttcgaa
    10741 taattagtag gtctcattaa aaacatcaca aattaagagt aaagcaataa aaatatagaa
    10801 atatgtagta taaaatagta ttctaggact aagattggtc atacctgcgg tttctatgtg
    10861 aatatgatgc gatgtgttgc ttccccacta tctacagggt atcgcactct acgcttacaa
    10921 agtctagagg atccactagt gtcgacgatg taggtcacag tctcgaagcc gcggtgcggg
    10981 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
    11041 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
    11101 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
    11161 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
    11221 tcgacactag ttctagccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
    11281 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    11341 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    11401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    11461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    11521 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    11581 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    11641 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    11701 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    11761 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    11821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    11881 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    11941 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    12001 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    12061 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    12121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    12181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    12241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    12301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    12361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    12421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    12481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    12541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg ggacccacgc
    12601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    12661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    12721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    12781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    12841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    12901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    12961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    13021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    13081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    13141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    13201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    13261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    13321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    13381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    13441 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
    13501 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
    13561 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
    13621 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
    13681 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
    13741 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
    13801 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
    13861 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
    13921 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    13981 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    14041 aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt acgtaccggg
    14101 cccctagtat gtatgtaagt taataaaacc catttttgcg gaaagtagat aaaaaaaaca
    14161 tttttttttt ttactgcact ggatatcatt gaacttatct g
//
