LOCUS       sequence-458646-       22275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level P vector for improved autonomous 
            bioluminescence in plants encoding kanamycin resistance cassette, 
            nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22275)
  AUTHORS   Shakhova ES, Karataeva TA, Markina NM, Mitiouchkina T, Palkina KA, 
            Perfilov MM, Wood MG, Hoang TT, Hall MP, Fakhranurova LI, Alekberova
            AE, Malyshevskaia AK, Gorbachev DA, Bugaeva EN, Pletneva LK, Babenko
            VV, Boldyreva DI, Gorokhovatsky AY, Balakireva AV, Gao F, Choob VV, 
            Encell LP, Wood KV, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     An improved pathway for autonomous bioluminescence imaging in 
            eukaryotes.
  JOURNAL   Nat Methods. 2024 Mar;21(3):406-410. doi: 
            10.1038/s41592-023-02152-y. Epub 2024 Jan 22.
  PUBMED    38253843
REFERENCE   2  (bases 1 to 22275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-02152-y"; journalName: "Nat Methods"; date: 
            "2024-03"; volume: "21"; issue: "3"; pages: "406-410"
COMMENT     Sequence Label: pNK497
FEATURES             Location/Qualifiers
     source          1..22275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    28..52
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(175..763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(255..274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(934..1728)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(1729..1833)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2116..2310
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2376..3449)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3878..4507)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4771..4795
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4979..5158
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5189..5243
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5245..6039
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5299..5318)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5909..5928
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6066..6773
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        6873..7218
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      12412..13119
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        16155..16500
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(17061..17072)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        17994..18339
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      19357..20064
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     terminator      21981..22233
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cacgaagtgg tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
       61 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
      121 tccatttgta tgtgccaacc acaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      181 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      241 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      301 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      361 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      421 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      481 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      541 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      601 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      661 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      721 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      781 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      841 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      901 ctaaagtata tatgagtaaa cttggtctga cagctaaaac aattcatcca gtaaaatata
      961 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
     1021 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
     1081 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
     1141 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
     1201 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcctctt cccagttttc
     1261 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
     1321 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
     1381 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
     1441 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
     1501 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
     1561 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
     1621 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatcttttg cgcagcggta
     1681 tttttcgatc agttttttca attccggtga tattctcatt ttagccatac tcttcctttt
     1741 tcaatattat tgaagcattt atcaaggtta ttgtctcatg agcggataca tatttgaatg
     1801 tatttagaaa aataaacaaa taggggttcc gcgcacgaat tggccagcgc tgccattttt
     1861 ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag ctcggtgcct
     1921 tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg
     1981 cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg
     2041 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg
     2101 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
     2161 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
     2221 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
     2281 tttgggttcc caatgtacgt gctatccaca ggaaagaagc tttttcgacc tttttcccct
     2341 gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc
     2401 tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc
     2461 aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc
     2521 ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct
     2581 gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc
     2641 aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg
     2701 tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg
     2761 atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg
     2821 gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc
     2881 aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg
     2941 tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat
     3001 tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg
     3061 gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca
     3121 gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg
     3181 cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc
     3241 ggcttcctaa tcgacgacgc accggctgcc ggcggttgcc gggattcttt gcggattcga
     3301 tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
     3361 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
     3421 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
     3481 cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
     3541 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
     3601 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
     3661 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
     3721 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
     3781 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
     3841 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
     3901 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
     3961 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
     4021 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
     4081 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
     4141 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
     4201 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
     4261 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
     4321 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
     4381 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
     4441 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
     4501 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
     4561 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
     4621 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
     4681 gatttcatgc agccgcacgg ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
     4741 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt
     4801 gacgcttaga caacttaata acacattgcg gacgttttta atgtactggg gtggatgcag
     4861 tgggccccac tctgtggaag acaatgccga attcggatcc ggagcggaga attaagggag
     4921 tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga
     4981 accgcaacgt tgaaggagcc actgagccgc gggtttctgg agtttaatga gctaagcaca
     5041 tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa
     5101 tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga
     5161 gtctcatatt cactctccta tttttacaac aattaccaac aacaacaaac aacaaacaac
     5221 attacaatta catttacaat taccatggtt gaacaagatg gattgcacgc aggttctccg
     5281 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5341 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5401 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     5461 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5521 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5581 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5641 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5701 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5761 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgactcatgg cgatgcctgc
     5821 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     5881 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     5941 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6001 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgcta
     6061 gagtcctgct ttaatgagat atgcgagacg cctatgatcg catgatattt gctttcaatt
     6121 ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc
     6181 ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg
     6241 ttcaatttac tgattgtacc ctactactta tatgtacaat attaaaatga aaacaatata
     6301 ttgtgctgaa taggtttata gcgacatcta tgatagagcg ccacaataac aaacaattgc
     6361 gttttattat tacaaatcca attttaaaaa aagcggcaga accggtcaaa cctaaaagac
     6421 tgattacata aatcttattc aaatttcaaa agtgccccag gggctagtat ctacgacaca
     6481 ccgagcggcg aactaataac gctcactgaa gggaactccg gttccccgcc ggcgcgcatg
     6541 ggtgagattc cttgaagttg agtattggcc gtccgctcta ccgaaagtta cgggcaccat
     6601 tcaacccggt ccagcacggc ggccgggtaa ccgacttgct gccccgagaa ttatgcagca
     6661 tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca gtgacgacaa
     6721 atcgttgggc gggtccaggg cgaattttgc gacaacatgt cgaggctcag ccgctgcaag
     6781 aattcaagct tggaggtcaa catggtggag cacgacactc tggtctactc caaaaatgtc
     6841 aaagatacag tctcagaaga tcaaagggct attgagactt ttcaacaaag gataatttcg
     6901 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa
     6961 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat
     7021 ctctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     7081 gaagaggttc caaccacgtc tacaaagcaa gtggattgat gtgacatctc cactgacgta
     7141 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
     7201 tttcatttgg agaggacacg ctcgagtata agagctcatt tttacaacaa ttaccaacaa
     7261 caacaaacaa caaacaacat tacaattaca tttacaatta tcgatacaat gaactcaagt
     7321 aagaatccgc cctccacatt gttggatgta tttctcgaca cggctagaaa tcttgatacc
     7381 gctagtagga atgtcctgga atgcggggaa catcgttggt catatcgtga attggatacg
     7441 gtaagctctg ccctggccca gcaccttcga tataccgtcg gcttgagtcc tacagtagcc
     7501 gtgatcagtg agaaccaccc ttacatattg gccttgatgt tggccgtttg gaaattgggg
     7561 ggtaccttcg ctccaatcga cgtacatagt cccgccgagc tcgtggctgg catgttgaat
     7621 atagttagcc ccagctgtct ggttatacct tctagtgacg ttaccaacca gacattggcc
     7681 tgcgatctca atataccagt agtagctttc caccctcatc aatcaacgat accggaactg
     7741 aacaaaaagt acttgactga cagtcaaata tcacctgact tgcctttccc cgaccccaac
     7801 aggccggctc tttatctgtt taccagcagc gctacttctc gttctaatct gaaatgcgtc
     7861 cccttgacgc atacatttat ccttagaaac tctctttcaa aaagggcctg gtgcaaacgt
     7921 atgaggcccg aaaccgattt tgatggtatt cgtgtactcg gttgggctcc ttggtcccac
     7981 gtgcttgcac atatgcagga cattggacca ttgacgcttt tgaacgcagg gtgctacgtt
     8041 tttgctacga ctccttcaac atatccgaca gagctgaagg acgatcgaga tgtgatctcc
     8101 tgtgccgcca acgcagtcat gtataaggga gttaagtcat ttgcctgtct accttttgtg
     8161 ttgggcggac ttaaagcact ctgcgaaagc gagccatctg taaaagctca gttgcaagtg
     8221 gaagagcgag cacaattgct caaatccctg cagcatatga agatcctgga gtgcggtggt
     8281 gccatgttgg aagtctccgt tgcatcctgg gcaatagaga acagaatccc cataagcatt
     8341 gggataggca tgactgaaac tggtggagcc ctcttcgctg gaccagttca ggcaatacaa
     8401 acaggatttt catcagagga caagttcata gaggacgcca cttatctttt ggtgaaggat
     8461 gactatgagt cccacgccga ggaagatatt aacgaaggtg aactcgtcgt caagtccagg
     8521 atgctgcctc gtgggtatct ggggtataat gatccatcat tcagcgttga tgatgcaggc
     8581 tgggtgacgt tcaaaacggg agataggtac agtgttacac ccgacggcaa atttagctgg
     8641 ctggggagaa ataccgactt cattcagatg acatctggtg aaaccttgga ccctcgtccg
     8701 atagaaagtc tcctctgtga gtcctcactt atttctaggg cctgcgtaat tggcgataag
     8761 tttcttaacg ggccagcaac tgctgtttgt gccataatcg aactggaacc aaccaccgtt
     8821 gagaagggtc aagctcattc cagagacata gctaggatat tcgcccccat caacagggat
     8881 ttgccccccc ctttgcgtat cgcatggtca catgtcctgg ttttgcagcc tagtgagaag
     8941 atacctatga caaagaaggg gacgattttt cgaaaaaaga tcgaacaagt ctttgggtcc
     9001 gcattgggtg gatcatccgg cgacaatagc caagctacta ctgacgctag cgtggtaaga
     9061 cgtgacgaac tcagtaacac agtgaagcac attatatccc gagtgttggg ggtgtctgat
     9121 gacgaactct tgtggaccct ctctttcgcc gagttgggta tgacttctgc tttggccacc
     9181 aggattgcaa atgagttgaa cgaagtcctt gtgggagtga acttgcctat aaacgcctgc
     9241 tatattcatg tggatcttcc gagcctcagt aatgcagtct atgccaaact cgcacacttg
     9301 aagttgccag accgtactcc ggaaccacga aaggcaccag ttgaaaatcc cggaggtaaa
     9361 gagatcgtca ttgtaggcca ggccttccgt ttgcccggga gtatcaacga cgtcgcctct
     9421 cttagggatg cctttttggc taggcaggcc agctcaatta ttaccgagat acctccggac
     9481 aggtgggacc acgcatcctt ctacccaaaa gatattcgtt tcaataaggc tgggctggtg
     9541 gacatcgcca actacgatca ttccttcttt gggctgaccg ccacggaagc actctatctc
     9601 agccctacga tgcgactcgc cttggaggtg tcatttgaag cattggagaa tgccaacatt
     9661 ccagtttctc aactgaaggg ctcccagaca gcagtgtatg ttgcaacaac agacgatggt
     9721 tttgaaacgt tgctcaacgc cgaagcaggt tacgatgcct acaccagatt ttacgggact
     9781 gggagggctg cctctacggc atcaggccgt ataagttatc tcctcgatgt acatggacca
     9841 tccatcactg tggacacagc ctgcagtgga ggagcagtgt gcatagatca agccattgat
     9901 tatctccagt cctcaagtgc tgctgatacc gctattattt gtgctagtaa tacccattgc
     9961 tggccggggt catttatgtt tctcagcgct caaggtatgg tttcatctgg gggtagatgt
    10021 gctacattca cgactgatgc agacggatat gttccatccg aaggagctgt agctttcata
    10081 cttaagacaa gagaggctgc tatgagagat aaagatacca tattggcaac aataaaggct
    10141 acgcaaattt cccacaacgg acgatcccaa ggactcgtgg ctcctaacgt taatagtcaa
    10201 gccgatctgc atcgtagcct ccttcagaaa gccgggcttt cccctgccga tatacatttc
    10261 atagaagcac acggcactgg gacaagtctc ggagacttgt ctgaaatcca agccattaac
    10321 gacgcttata cctctagtca acccagaaca gccggaccat tgattgtgtc cgcctccaaa
    10381 actgtgatcg ggcacactga acctgctgga ccattggtcg gtatgctctc tgtactcaat
    10441 tcctttaagg aaggggctgt cccggggctt gcccacctta ccgccgacaa cttgaatccg
    10501 gctttggatt gctctagtgt ccctttgttg attccgtatc aaccggtaca tttggctgca
    10561 cccaagccac atagggccgc tgttatgagt tatgggttct ccggtacact gggagggatc
    10621 gtgttggaag ctccggatga agagcgtctt gaagaagaac ccccaaacga caagccaatg
    10681 ttgtttgtag ttagtgctaa gacccatacc gccctgatcg agtatttggg tagatatttg
    10741 gagtttttgc ttcaagccaa cccgcaagat ttctgcgaca tatgttacac atcatgcgtc
    10801 ggccgtgaac attataggta ccgtttcgct tgcgtcgcca acgacatgga ggacctgatc
    10861 ggccaacttc aaaaaaggct ggggtccaag gtaccgccaa aaccgagcta taagcgaggc
    10921 gcacttgctt ttgcattctc cggtcaaggt actcagttta gaggtatggc aactgaactg
    10981 gcaaaagcct actcaggttt taggaagata gtcagtgact tggctaagag ggcctccgaa
    11041 ctctccggcc atgctattga taggttcttg ttggcttatg atattggcgc tgaaaatgtg
    11101 gcaccagact cagaagcaga ccaaatatgc atattcgtgt accaatgcag cgtgctcaga
    11161 tggcttcaga ccatgggcat tcgtcctagt gctgtaatag gtcactcact gggagaaatc
    11221 tctgcttcag ttgcagcagg ggcactcagt ctcgacagcg ccctggatct cgtgataagc
    11281 agggctagat tgttgagatc ttctactaat gcccccgctg gtatggctgc catgtctgct
    11341 agccaggatg aggtcgtgga actcatcgga aaattggacc ttgataaagc taactccttg
    11401 tcagtctcag tcatcaatgg cccccagaac accgttgtgt ccggtagctc agcagccatt
    11461 gaatcaatag tagcattggc caagggtagg aaaattaaag caagcgctct taatattaat
    11521 caggcatttc actccccata tgttgattct gcagtaccgg gcttgcgagc ctggtcagaa
    11581 aagcatatct catccgcaag gccgctccag ataccgttgt attcaacctt gcttggtgct
    11641 caagtcagcg aagggcaaat gcttaatccg gatcactggg tagatcatgc ccgtaagcct
    11701 gttcagtttg ctcaagccgc aacaatcatg aaagagtcct ttactggggt gatcatcgac
    11761 atcggtcccc aggtggttgc ctggtcactg ctcttgagca acggtttgac atccgtcact
    11821 gcactcgccg ctaagcgtgg gcgaagccaa caggtcgctt tcctcagcgc cttggccgat
    11881 ctgtatcaag actatggtgt agtacctgac tttgtcgggt tgtacgccca gcaggaggac
    11941 gctagcaggc ttaaaaagac agatattttg acctacccgt ttcaaagggt cagacgatat
    12001 ccgtctttca ttccgtcacg tcgtgcaccg acacatgccc acgttcagga cgaggagact
    12061 ctgtcatccg ggagcagtac gcccacattg gaaaataccg accttgattc tggtaaggag
    12121 agtctcatgg gaccgacaag agggttgttg cgtgtcgacg acttgaggga ctccatcgtt
    12181 agttctgtga aggacgtact cgaactcaaa tccaacgagg acctcgattt gtcagaatca
    12241 ttgaacgctc ttgggatgga ctccatcatg ttcgcacagt tgagaaagag gataggcgaa
    12301 gggcttggat tgagtgttcc tatggttttt ctgtcagacg ctttctccat aggtgaaatg
    12361 gttagcaacc tggtggaaca ggctgaggct tccgaggaca actagcttgt cctgctttaa
    12421 tgagatatgc gagaagccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt
    12481 gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg
    12541 aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca atttactgat
    12601 tgtaccctac tacttatatg tacaatatta aaatgaaaac aatatattgt gctgaatagg
    12661 tttatagcga catctatgat agagcgccac aataacaaac aattgcgttt tattattaca
    12721 aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat tacataaatc
    12781 ttattcaaat ttcaaaagtg ccccaggggc tagtatctac gacacaccga gcggcgaact
    12841 aataacgctc actgaaggga actccggttc cccgccggcg cgcatgggtg agattccttg
    12901 aagttgagta ttggccgtcc gctctaccga aagttacggg caccattcaa cccggtccag
    12961 cacggcggcc gggtaaccga cttgctgccc cgagaattat gcagcatttt tttggtgtat
    13021 gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt
    13081 ccagggcgaa ttttgcgaca acatgtcgag gctcagcagg accgctacta gaattcgagc
    13141 tcggagtggc agacatactg tcccacaaat gaagatggaa tctgtaaaag aaaacgcgtg
    13201 aaataatgcg tctgacaaag gttaggtcgg ctgcctttaa tcaataccaa agtggtccct
    13261 accacgatgg aaaaactgtg cagtcggttt ggctttttct gacgaacaaa taagattcgt
    13321 ggccgacagg tgggggtcca ccatgtgaag gcatcttcag actccaataa tggagcaatg
    13381 acgtaagggc ttacgaaata agtaagggta gtttgggaaa tgtccactca cccgtcagtc
    13441 tataaatact tagcccctcc ctcattgtta agggagcaaa atctcagaga gatagtccta
    13501 gagagagaaa gagagcaagt agcctagaag tagtcaaggc ggcgaagtat tcaggcaggt
    13561 ggccaggaag aagaaaagcc aagacgacga aaacaggtaa gagctaagct taatggtcca
    13621 agacacctca agcgctagta ccagtccaat tttgacaaga tggtatatag atactagacc
    13681 tttgactgct tctaccgcag ccttgccact gttggaaact ctgcagcctg cagaccagat
    13741 aagtgtccaa aagtactacc atctgaagga taagcatatg tctctcgcat ccaatcttct
    13801 caagtacttg tttgttcaca ggaattgccg aatcccatgg tctagcattg taatatcaag
    13861 aactccagat ccccatagaa ggccctgtta tataccaccc tctggttctc aagaggacag
    13921 ctttaaggat ggctacactg gaattaatgt cgagttcaac gtatcccatc aagcaagcat
    13981 ggtggcaata gcaggcactg catttacacc caactctgga ggtgattcca aacttaagcc
    14041 agaggttggc atagacatta cttgcgtgaa tgagcgacag gggcgaaacg gtgaggagcg
    14101 atcccttgaa agtttgaggc agtacataga tattttctca gaggtttttt ccaccgcaga
    14161 gatggcaaac atcagaagat tggatggggt gtcatcaagc tccttgagcg ccgataggtt
    14221 ggtggactat ggttaccgat tgttttacac ttattgggct cttaaggaag catacatcaa
    14281 gatgacaggg gaagccctgc tggcaccatg gctcagggag ttggaattta gtaatgtcgt
    14341 tgccccagca gcagtggccg aatccggaga ttccgccgga gactttggag agccttatac
    14401 cggggtacga acaacccttt acaaaaactt ggtcgaggat gttaggatag aggtggctgc
    14461 tctgggaggt gactacttgt tcgccactgc tgcacgaggg ggcggtatag gtgcctccag
    14521 ccgaccaggg ggaggccctg atggctcagg aattaggagt caagatccct ggcgaccatt
    14581 taaaaagctc gacatagaga gagacataca gccttgcgcc actggagtct gtaattgtct
    14641 gtctcgaggt agttaactag cttaccgcac tgtgtgtggt ttctcaagac caagacagct
    14701 aaagcctaaa gtcagagatc taatatgtgt attgttattc atgacaccac agctgccact
    14761 tttggtgtta tgatctgttt gtagaagtag gaattctttt ttttctactt aataatagct
    14821 taaagagctg tgcaatttgg tctgtatttt ttgtgtattt tgcactcatt atttgtgaac
    14881 agtttgagaa ctatttattt tctaagattt gtgcacgtat gaaccacttt tcatctatat
    14941 accaccatgt ttattctgca tctatgggat tgagtttgaa tattcgttga tcaacaaagt
    15001 tatatttggt ggatactact tgaaggtgca tatactttgt gctcatatat ttagttgata
    15061 ttctggattt tgagctggac aaattgatca aggtagtcta atctggtctg gttactaata
    15121 aaactcaaga gatcactgtc tcgctttacg aattcccatg gggaggaatt ccaatcccac
    15181 aaaaatctga gcttaacagc acagttgctc ctctcagagc agaatcgggt attcaacacc
    15241 ctcatatcaa ctactacgtt gtgtataacg gtccacatgc cggtatatac gatgactggg
    15301 gttgtacaaa ggcggcaaca aacggcgttc ccggagttgc acacaagaaa tttgccacta
    15361 ttacagaggc aagagcagca gctgacgcgt acacaacaag tcagcaaaca gacaggttga
    15421 acttcatccc caaaggagaa gctcaactca agcccaagag ctttgctaag gccctaacaa
    15481 gcccaccaaa gcaaaaagcc cactggctca cgctaggaac caaaaggccc agcagtgatc
    15541 cagccccaaa agagatctcc tttgccccgg agattacaat ggacgatttc ctctatcttt
    15601 acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc actgataatg
    15661 agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt agagaggcct
    15721 acgcagcaag tctcatcaag acgatctacc cgagtaacaa tctccaggag atcaaatacc
    15781 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc aagaacacag
    15841 agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
    15901 ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt cctactgaat
    15961 ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact cgccgtcaag
    16021 actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa aatcttcgtc
    16081 aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa
    16141 gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc
    16201 cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac
    16261 aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc cgacagtggt
    16321 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt tccaaccacg
    16381 tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga cgcacaatcc
    16441 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
    16501 cgctcgagta taagagctca tttttacaac aattaccaac aacaacaaac aacaaacaac
    16561 attacaatta catttacaat tatcgataca atgaggtcta ctatctcttt gtctagtctc
    16621 cttgagagac tgagcaaatt gtcatcccga tccatcgcaa ttacctgcgg cgtagtcttg
    16681 gcaagtgcaa tagcattccc aattatccga agagactatc aaacgttcct tgaagtcggt
    16741 ccgagctatg ccccacagaa cttccgaggc tatatcactg tttgtgtctt gtcacttttt
    16801 aggcaagagc agaaagggtt ggcaatctat gacaggcttc cagaaaagag gcgttggctt
    16861 gccgacttgc cgtttcgtga agggccaagg ccatccataa cctcccacat tatacagcgt
    16921 cagcgtactc agctggtaga tcaagagttt gcaaccagag aacttatcga taaggtgatc
    16981 ccacgtgtgc aagcaagaca cacagacaaa acttttctca gtacgtcaaa atttgagttt
    17041 catgcaaagg ccatcttcct tctcccctct atccctatta atgatcctct taacattccc
    17101 tcacacgata cggtaagaag aaccaagcgt gagatcgctc acatgcatga ttaccatgat
    17161 tgctctctgc acttggctct tgctcctcag gatggtaagg aagttttgaa gaaggggtgg
    17221 ggccagcgtc acccactggc cggaccagga gtcccaggcc ctcctactga gtggaccttc
    17281 ctttacgcac caaggaacga agaggaggcc agagttgtcg agatgatagt cgaagctagt
    17341 attggctaca tgactaacga tcctgctggt aagattgtcg aaaatgccaa aggtagttaa
    17401 ctagcttgct ctcaagatca aaggcttaaa aagctggggt tttatgaatg ggatcaaagt
    17461 ttcttttttt cttttatatt tgcttctcca tttgtttgtt tcatttccct ttttgttttc
    17521 gtttctatga tgcacttgtg tgtgacaaac tctctgggtt tttacttacg tctgcgtttc
    17581 aaaaaaaaaa accgctttcg ttttgcgttt tagtcccatt gttttgtagc tctgagtgat
    17641 cgaattgatg cctctttatt ccttttgttc cctataattt ctttcaaaac tcagaagaaa
    17701 aaccttgaaa ctctttgcaa tgttaatata agtattgtat aagattttta ttgatttggt
    17761 tattagtctt acttttgcta cctccatctt cacttggaac tgatattctg aatagttaaa
    17821 gcgttacatg tgttccattc acaaatgaac ttaaactagc acaaagtcag atattttaag
    17881 atcgcaccat ttcgctcaga gaattcgcat gcggaggtca acatggtgga gcacgacact
    17941 ctggtctact ccaaaaatgt caaagataca gtctcagaag atcaaagggc tattgagact
    18001 tttcaacaaa ggataatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac
    18061 ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa
    18121 ggaaaggcta tcattcaaga tctctctgcc gacagtggtc ccaaagatgg acccccaccc
    18181 acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca agtggattga
    18241 tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
    18301 tcctctatat aaggaagttc atttcatttg gagaggacac gctcgagtat aagagctcat
    18361 ttttacaaca attaccaaca acaacaaaca acaaacaaca ttacaattac atttacaatt
    18421 atcgatacaa tggcacctat ttcatccacg tggagcaggt tgataaggtt cgtggctgta
    18481 gaaacttccc tggttcatat tggtgagcca attgatgcaa cgatggacgt tggcctcgcc
    18541 cgtagagaag gtaagacaat tcaagcatac gaaataatcg gaagcggcag tgccctcgat
    18601 ctttctgcac aagtctctaa gaacgtactc acagttaggg aattgttgat gccgctcagt
    18661 cgagaagaga ttaagaccgt tcgatgcctc ggcttgaact atccggtcca cgccacagaa
    18721 gctaacgtgg ccgtgccgaa gtttcctaac ctgttctata agcccgtaac atcattgatt
    18781 ggtccaggcg gtcttattac tattccctcc gtcgttcaac cccccaagga gcaccagtca
    18841 gattacgagg ctgagctggt tatagtaata ggtaaggcag caaagaacgt atcagaggat
    18901 gaggctttgg attatgtatt gggctatacc gctgcaaacg atatatcttt ccgtaagcat
    18961 caattggctg tcagtcaatg gagcttcagc aagggctttg atggaactaa tccacttggt
    19021 ccctgcttgg tttccgcctc cagtatccct gacccacagg atattccaat acagtgcaaa
    19081 ttgaatggtg gagtcgtcca aaatggcaac accagagatc aaatcttcaa cgttaagaaa
    19141 accatatcat ttctttccca ggggactact ttggagcccg gttccattat ccttaccggg
    19201 accccagatg gggtggggtt cgtacgaaac ccgcccctgt atttgaagga tggcgatgag
    19261 gttatgactt ggatcggtag tggaatcgga actctcgcaa atacagttcg agaagaacaa
    19321 acgtgctttg catcaggcgg ccacgaatag cttgtcctgc tttaatgaga tatgcgagaa
    19381 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc
    19441 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt
    19501 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt
    19561 atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct
    19621 atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa
    19681 aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa
    19741 aagtgcccca ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga
    19801 agggaactcc ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc
    19861 cgtccgctct accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta
    19921 accgacttgc tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga
    19981 agtgcaggtc aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg
    20041 cgacaacatg tcgaggctca gcaggaccgc ttgtggaatt cctcgaggga gtcatcaaaa
    20101 tatttagcag cattccagat tgggttcaat caacaaggta cgagccatat cactttattc
    20161 aaattggtat cgccaaaacc aagaaggaac tcccatcctc aaaggtttgt aaggaagaat
    20221 tctcagtcca aagcctcaac aaggtcaggg tacagagtct ccaaaccatt agccaaaagc
    20281 tacaggagat caatgaagaa tcttcaatca aagtaaacta ctgttccagc acatgcatca
    20341 tggtcagtaa gtttcagaaa aagacatcca ccgaggactt aaagttagtg ggcatctttg
    20401 aaagtaatct tgtcaacatc gagcagctgg cttgtgggga ccagacaaaa aaggaatggt
    20461 gcagaattgt taggcgcacc taccaaaagc atctttgcct ttattgcaaa gataaagcag
    20521 attcctctag tacaagtggg gaacaaaata acgtggaaaa gagctgtcct gacagcccac
    20581 tcactaatgc gtatgacgaa cgcagtgacg accacaaaag aattccctct atataagaag
    20641 gcattcattc ccatttgaag gatcatcaga tactgaacca atatttctca atggcctctt
    20701 ttgaaaattc actctctgtg ttgatcgtgg gagccggttt gggtggtcta gcagctgcta
    20761 tcgctctccg acgacagggc cacgtggtta agatctacga atcatctagc tttaaggccg
    20821 agctcggcgc aggcttggca gttccaccga atacactgcg tagtctacag caattgggtt
    20881 gtaacactga aaaccttaac ggcgttgata atctctgttt tactgccatg ggatatgatg
    20941 ggtcagtggg catgatgaac aatatgactg attaccgtga agcatatggc actagttgga
    21001 tcatggttca tcgtgtcgat ttgcataacg agttgatgag agttgctttg gacccagggg
    21061 gattgggccc tcccgcaacg cttcacctga atcatcgagt cacgttttgt gatgtagatg
    21121 cttgtacggt gacatttacc aacggtacaa ctcaaagtgc agatcttatc gttggggctg
    21181 acgggatacg ttcaactatc cgtagatttg tcttggagga ggacgtgacc attcccgcaa
    21241 gcggaattgt ggggtttcga tggttggtac aggctgatgc cctcgatcca tatcctgaac
    21301 tcgattggat agtcaaaaag ccaccgctcg gagctaggtt gatttctacg ccccagaatc
    21361 cacaatctgg agtgggactg gcagatcgta gaactattat tatatacgct tgtcgtgggg
    21421 ggacaatggt aaatgtgctg gccgtacacg atgacgagcg agatcagaat actgcagatt
    21481 ggagcgttcc agcatcaaag gatgacttgt ttcgagtatt ccatgactac catccgagat
    21541 tcagacggct cttggaattg gctcaagata taaatctctg gcagatgagg gttgtgccgg
    21601 tgctcaagaa atgggtgaac aaacgagtgt gcctgcttgg agatgccgct catgccatgc
    21661 ttccgactct cggccagggt ttcgggatgg gattggagga cgccgtagcc cttggtactc
    21721 tcttgccaaa aggtacgact gctagtcaaa tagagacaag actggctgtc tacgaacagc
    21781 ttagaaagga ccgagcagag tttgtagccg ctgaaagtta tgaggagcag tatgtccccg
    21841 agaagagggg cttgtacttg cgtagcaagg aacttagaga tagagtcatg gggtatgaca
    21901 taaaagtgga gtctgagaag gtactggaaa ccttgcttag gagctctaac tcagcaggta
    21961 gttaactagc ttgtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga
    22021 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
    22081 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
    22141 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
    22201 tatcgcgcgc ggtgtcatct atgttactag atcgacgctg agcttgtctt cgaggatgca
    22261 catgtgaccg aggga
//
