LOCUS       sequence-458690-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter 
            with upstream open reading frame.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Wang NB, Lende-Dorn BA, Beitz AM, Han P, Adewumi HO, O'Shea TM, 
            Galloway KE
  TITLE     Proliferation history and transcription factor levels drive direct 
            conversion to motor neurons.
  JOURNAL   Cell Syst. 2025 Apr 16;16(4):101205. doi: 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13.
  PUBMED    40086434
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-04-16"; volume: "16(4):101205. doi"; pages: " 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13"
COMMENT     Sequence Label: pMXs-uORFwx1-mNgn2x3HA-P2A-mRuby2
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             829..855
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             865..891
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             901..927
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgttcgtca aatctgagac tctggagttg aaggaggaag aggaggtact gatgctgctg
       61 ggctcggctt ccccggcctc ggcgaccctg accccgatgt cctccagcgc ggacgaggag
      121 gaggacgagg agctgcgccg gccgggctcc gcgcgtgggc agcgtggagc ggaagccggg
      181 cagggggtgc agggcagtcc ggcgtcgggt gccgggggtt gccggccagg gcggctgctg
      241 ggcctgatgc acgagtgcaa gcgtcgcccg tcgcgctcac gggccgtctc ccgaggtgcc
      301 aagacggcgg agacggtgca gcgcatcaag aagacccgca ggctcaaggc caacaaccgc
      361 gagcgcaacc gcatgcacaa cctaaacgcc gcgctggacg cgctgcgcga ggtgctgccc
      421 accttccccg aggatgccaa gctcacgaag atcgagacgc tgcgcttcgc ccacaattac
      481 atctgggcgc tcaccgagac tctgcgcctg gcggaccact gcgccggcgc cggtggcctc
      541 cagggggcgc tcttcacgga ggcggtgctc ctgagcccgg gagctgcgct cggcgccagc
      601 ggggacagcc cttctccacc ttcctcctgg agctgcacca acagcccggc gtcatcctcc
      661 aactccacgt ccccatacag ctgcacttta tcgcccgcta gccccgggtc agacgtggac
      721 tactggcagc ccccacctcc ggagaagcat cgttatgcgc ctcacctgcc cctcgccagg
      781 gactgtatcg ggtctggctc cggatcaagt ggtggcagcg gatcaggata tccatacgat
      841 gtaccagact atgcgggatc gggttacccc tacgacgtcc ccgactacgc cggctccggc
      901 tacccctacg acgtccctga ttatgct
//
