LOCUS       sequence-458714-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Isl1 with mRuby2 fluorescent reporter with 
            upstream open reading frame.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Wang NB, Lende-Dorn BA, Beitz AM, Han P, Adewumi HO, O'Shea TM, 
            Galloway KE
  TITLE     Proliferation history and transcription factor levels drive direct 
            conversion to motor neurons.
  JOURNAL   Cell Syst. 2025 Apr 16;16(4):101205. doi: 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13.
  PUBMED    40086434
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-04-16"; volume: "16(4):101205. doi"; pages: " 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13"
COMMENT     Sequence Label: pMXs-uORFx2-Isl1-P2A-mRuby2
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      791..800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgggagaca tgggcgatcc accaaaaaaa aaacgtctga tttccctgtg tgttggttgc
       61 ggcaatcaaa ttcacgacca gtatattctg agggtttctc cggatttgga gtggcatgca
      121 gcatgtttga aatgtgcgga gtgtaatcag tatttggacg aaagctgtac gtgctttgtt
      181 agggatggga aaacctactg taaaagagat tatatcaggt tgtacgggat caaatgcgcc
      241 aagtgcagca taggcttcag caagaacgac ttcgtgatgc gcgcccgctc taaggtgtac
      301 cacatcgagt gtttccgctg tgtagcctgc agccgacagc tcatcccggg agacgaattc
      361 gccctgcggg aggatgggct tttctgccgt gcagaccacg atgtggtgga gagagccagc
      421 ctgggagctg gagatcctct cagtcccttg catccagcgc ggcctctgca aatggcagcc
      481 gaacccatct cggctaggca gccagctctg cggccgcacg tccacaagca gccggagaag
      541 accacccgag tgcggactgt gctcaacgag aagcagctgc acaccttgcg gacctgctat
      601 gccgccaacc ctcggccaga tgcgctcatg aaggagcaac tagtggagat gacgggcctc
      661 agtcccagag tcatccgagt gtggtttcaa aacaagcggt gcaaggacaa gaaacgcagc
      721 atcatgatga agcagctcca gcagcagcaa cccaacgaca aaactaatat ccaggggatg
      781 acaggaactc ccatggtggc tgctagtccg gagagacatg atggtggttt acaggctaac
      841 ccagtagagg tgcaaagtta ccagccgccc tggaaagtac tgagtgactt cgccttgcaa
      901 agcgacatag atcagcctgc ttttcagcaa ctggtcaatt tttcagaagg aggaccaggc
      961 tctaattcta ctggcagtga agtagcatcg atgtcctcgc agctcccaga tacacccaac
     1021 agcatggtag ccagtcctat tgaggca
//
