LOCUS       sequence-458741-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of HRAS(G12V).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Wang NB, Lende-Dorn BA, Beitz AM, Han P, Adewumi HO, O'Shea TM, 
            Galloway KE
  TITLE     Proliferation history and transcription factor levels drive direct 
            conversion to motor neurons.
  JOURNAL   Cell Syst. 2025 Apr 16;16(4):101205. doi: 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13.
  PUBMED    40086434
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-04-16"; volume: "16(4):101205. doi"; pages: " 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13"
COMMENT     Sequence Label: pMXs-HRAS(GV12)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..33,34..36,37..570)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
ORIGIN
        1 atgacggaat ataagctggt ggtggtgggc gccgtcggtg tgggcaagag tgcgctgacc
       61 atccagctga tccagaacca ttttgtggac gaatacgacc ccactataga ggattcctac
      121 cggaagcagg tggtcattga tggggagacg tgcctgttgg acatcctgga taccgccggc
      181 caggaggagt acagcgccat gcgggaccag tacatgcgca ccggggaggg cttcctgtgt
      241 gtgtttgcca tcaacaacac caagtctttt gaggacatcc accagtacag ggagcagatc
      301 aaacgggtga aggactcgga tgacgtgccc atggtgctgg tggggaacaa gtgtgacctg
      361 gctgcacgca ctgtggaatc tcggcaggct caggacctcg cccgaagcta cggcatcccc
      421 tacatcgaga cctcggccaa gacccggcag ggagtggagg atgccttcta cacgttggtg
      481 cgtgagatcc ggcagcacaa gctgcggaag ctgaaccctc ctgatgagag tggccccggc
      541 tgcatgagct gcaagtgtgt gctctcctga
//
