LOCUS       sequence-458790-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for HRAS(G12V) and SNAP-p53DD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Wang NB, Adewumi HO, Lende-Dorn BA, Beitz AM, O'Shea TM, Galloway KE
  TITLE     Compact transcription factor cassettes generate functional, 
            engraftable motor neurons by direct conversion.
  JOURNAL   Cell Syst. 2025 Mar 10:101206. doi: 10.1016/j.cels.2025.101206.
  PUBMED    40086435
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Mar 10:101206. doi: 10.1016/j.cels.2025.101206."
COMMENT     Sequence Label: pENTR-HRAS(G12V)-IRES-SNAP-p53DD
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..546
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 atggacaaag actgcgaaat gaagcgcacc accctggata gccctctggg caagctggaa
       61 ctgtctgggt gcgaacaggg cctgcaccgt atcatcttcc tgggcaaagg aacatctgcc
      121 gccgacgccg tggaagtgcc tgccccagcc gccgtgctgg gcggaccaga gccactgatg
      181 caggccaccg cctggctcaa cgcctacttt caccagcctg aggccatcga ggagttccct
      241 gtgccagccc tgcaccaccc agtgttccag caggagagct ttacccgcca ggtgctgtgg
      301 aaactgctga aagtggtgaa gttcggagag gtcatcagct acagccacct ggccgccctg
      361 gccggcaatc ccgccgccac cgccgccgtg aaaaccgccc tgagcggaaa tcccgtgccc
      421 attctgatcc cctgccaccg ggtggtgcag ggcgacctgg acgtgggggg ctacgagggc
      481 gggctcgccg tgaaagagtg gctgctggcc cacgagggcc acagactggg caagcctggg
      541 ctgggtcctg caggcggatc catgactgcc atggaggagt cacagtcgga tatcagcctc
      601 aagagagcgc tgcccacctg cacaagcgcc tctcccccgc aaaagaaaaa accacttgat
      661 ggagagtatt tcaccctcaa gatccgcggg cgtaaacgct tcgagatgtt ccgggagctg
      721 aatgaggcct tagagttaaa ggatgcccat gctacagagg agtctggaga cagcagggct
      781 cactccagct acctgaagac caagaagggc cagtctactt cccgccataa aaaaacaatg
      841 gtcaagaaag tggggcctga ctcagactga
//
