LOCUS       sequence-458811-        1383 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of jRCaMP1b calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Wang NB, Adewumi HO, Lende-Dorn BA, Beitz AM, O'Shea TM, Galloway KE
  TITLE     Compact transcription factor cassettes generate functional, 
            engraftable motor neurons by direct conversion.
  JOURNAL   Cell Syst. 2025 Mar 10:101206. doi: 10.1016/j.cels.2025.101206.
  PUBMED    40086435
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Mar 10:101206. doi: 10.1016/j.cels.2025.101206."
COMMENT     Sequence Label: pMXs-jRCaMP1b
FEATURES             Location/Qualifiers
     source          1..1383
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..84
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..120
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(124..132,133..147)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 atgctgcaga acgagcttgc tcttaagttg gctggacttg atattaacaa gactggagga
       61 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
      121 cgggatctgt acgacgatga cgataaggat ctcgcaacaa tggtcgactc atcgcgacgt
      181 aagtggaata agtggggtca cgcagtcaga gctataggtc ggctgagctc agcgaacaac
      241 accgaaatga tgtacccagc ggatggtggt ctgcgtggtt acactcacat ggcgctgaaa
      301 gttgatggcg gcggtcacct gtcctgttct ttcgtgacca cctaccgctc caaaaagact
      361 gtcggcaaca ttaagatgcc tgccattcat tacgtcagcc accgtctgga gcgcctggag
      421 gagagcgata acgaaatgtt tgtcgtacag cgtgaacacg cagttgccaa gtttgtgggc
      481 ctgggtggtg gcggcggtac cggagggagc atgaactccc tgatcaagga gaacatgcgt
      541 atgaaagtgg ttctggaagg ctccgtaaac ggccaccagt tcaaatgcac tggtgaaggc
      601 gaaggcaacc cgtatatggg cacccagact atgcgtatca aagtgatcga gggtggtccg
      661 ctgccgtttg cgttcgacat cctggcgacg tcctttatgt atggctcccg taccttcatc
      721 aaatatccga aaggcatccc ggatttcttt aagcagtcct tcccggaagg ttttacctgg
      781 gaacgtgtga cccgttacga agacggcggc gtaattaccg ttatgcaaga cacgtctctg
      841 gaggatggct gcctggtgta tcacgtgcag gttcgcggtg tgaacttccc gagcaatggt
      901 gctgtaatgc aaaagaaaac caaaggttgg gagcctacgg actcccaact gactgaagag
      961 cagatcgcag aatttaaaga ggctttctcc ctatttgaca aggacgggga tgggacaata
     1021 acaaccaagg agatggggac ggtgatgcgg tctctggggc agaaccccac agaagcagag
     1081 ctgcaggaca tgatcaatga agtagatgcc gacggtgacg gcacaatcga cttccctgag
     1141 ttcctgatta tgatggcagg caaaatgaaa tacacagaca gtgaagaaga aattagagaa
     1201 gcgttcggcg tgtttgataa ggatggcaat ggctacatca gtgcagcaga gcttcgccac
     1261 gtgatgacaa accttggaga gaagttaaca gatgaagagg ttgatgaaat gatcagggaa
     1321 gcagacagcg atggggatgg tcaggtaaac tacgaagagt ttgtacaaat gatgacagcg
     1381 aag
//
