LOCUS       sequence-458820-        9427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer plasmid with EGFP reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Yang Q, Davidson BA, Pajic P, Chen X, Gokcumen O, Gao M, Neelamegham
            S
  TITLE     Tuning the tropism and infectivity of SARS-CoV-2 virus-like 
            particles for mRNA delivery.
  JOURNAL   Nucleic Acids Res. 2025 Feb 27;53(5):gkaf133. doi: 
            10.1093/nar/gkaf133.
  PUBMED    40037714
REFERENCE   2  (bases 1 to 9427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-02-27"; volume: "53(5):gkaf133. doi"; 
            pages: " 10.1093/nar/gkaf133"
COMMENT     Sequence Label: pLKO.1 EF1a-EGFP
FEATURES             Location/Qualifiers
     source          1..9427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..229
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          230..1168
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1125..1145
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1192..1201
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1198..1200,1201..1203,1204..1920)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKG"
     primer_bind     complement(1243..1264)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1504..1523)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1851..1872
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             1993..2226
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2298..2419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2335..2354)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2389..2408
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2459..2594
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2614..2633)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2615..2633)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2643..2660)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2643..2659)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2652..2674)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2801..3256
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2888..2907)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3098..3119
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3282..3386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3387..3455,3456..4247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3605..3624)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4418..5006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4907..4926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5160..5177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5294..5315
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5329..5334,5335..5352,5353..5359)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5367..5383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5372..5394
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5391..5407
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5391..5407
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5426..5446
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5428..5446
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5474..5700
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5701..5881
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5928..6053
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6546..6779
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6964..7008
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7306..7554
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7306..7326
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7477..7496
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aattcgtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
       61 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      121 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      181 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
      241 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
      301 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
      361 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
      421 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
      481 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
      541 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
      601 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
      661 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
      721 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
      781 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
      841 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
      901 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
      961 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     1021 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     1081 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     1141 ggttcaaagt ttttttcttc catttcaggt gtcgtgagga tcccaccggt cgccaccatg
     1201 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1261 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1321 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1381 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1441 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1501 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1561 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1621 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1681 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1741 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1801 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1861 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggataa
     1921 gtagcgcttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     1981 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     2041 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     2101 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     2161 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     2221 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     2281 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2341 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2401 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     2461 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     2521 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     2581 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     2641 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2701 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2761 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     2821 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2881 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2941 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3001 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3061 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3121 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3181 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3241 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3301 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     3361 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     3421 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     3481 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     3541 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     3601 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     3661 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     3721 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     3781 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3841 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3901 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3961 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4021 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4081 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4141 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4201 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4261 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4321 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4381 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4441 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4501 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4561 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4621 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4681 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4741 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4801 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4861 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4921 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4981 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5041 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5101 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     5161 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     5221 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     5281 agcgcaacgc aattaatgtg agttagctca ctcattggca ccccaggctt tacactttat
     5341 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5401 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     5461 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     5521 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     5581 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     5641 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     5701 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     5761 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     5821 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     5881 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     5941 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     6001 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     6061 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     6121 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     6181 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     6241 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     6301 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     6361 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     6421 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     6481 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     6541 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     6601 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     6661 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     6721 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     6781 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     6841 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     6901 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     6961 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     7021 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     7081 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     7141 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     7201 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     7261 cggtatcgat cacgagacta gcctcgagcg gccgccccct tcaccgaggg cctatttccc
     7321 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     7381 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     7441 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     7501 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggtgtg
     7561 ttgtaaatga gcacacaaaa tacacatgct aaaatattat attctatgac ctttataaaa
     7621 tcaaccaaaa tcttcttttt aataacttta gtatcaataa ttagaatttt tatgttcctt
     7681 tttgcaaact tttaataaaa atgagcaaaa taaaaaaacg ctagttttag taactcgcgt
     7741 tgttttcttc acctttaata atagctactc caccacttgt tcctaagcgg tcagctcctg
     7801 cttcaatcat tttttgagca tcttcaaatg ttctaactcc accagctgct ttaactaaag
     7861 cattgtcttt aacaactgac ttcattagtt taacatcttc aaatgttgca cctgattttg
     7921 aaaatcctgt tgatgtttta acaaattcta atccagcttc aacagctatt tcacaagctt
     7981 tcatgatttc ttcttttgtt aataaacaat tttccataat acatttaaca acatgtgatc
     8041 cagctgcttt ttttacagct ttcatgtctt ctaaaactaa ttcataattt ttgtctttta
     8101 atgcaccaat atttaatacc atatcaattt ctgttgcacc atctttaatt gcttcagaaa
     8161 cttcgaatgc ttttgtagct gttgtgcatg cacctagagg aaaacctaca acatttgtta
     8221 ttcctacatt tgtgcctttt aataattctt tacaatagct tgttcaatat gaattaacac
     8281 aaactgttgc aaaatcaaat tcaattgctt catcacataa ttgtttaatt tcagctttcg
     8341 tagcatcttg ttttaataat gtgtgatcta tatatttgtt tagtttcatt ttttctccta
     8401 tatattcatt tttaatttta attctttaat aatttcgtct actttaactt tagcgttttg
     8461 aacagattca ccaacaccta taaaataaat ttttagttta ggttcagttc cacttgggcg
     8521 aacagcaaat catgacttat cttctaaata aaattttagt aagtcttgtc ctggcatatt
     8581 atacattcca tcgatgtagt cttcaacatt aacaacttta agtccagcaa tttgagttaa
     8641 gggtgttgct ctcaatgatt tcattaatgg ttcaattttt aatttctttt cttctggttt
     8701 aaaattcaag tttaaagtga aagtgtaata tgcacccatt tctttaaata aatcttctaa
     8761 atagtctact aatgttttat tttgtttttt ataaaatcaa gcagcctctg ctattaatat
     8821 agaagcttgt attccatctt tatctctagc tgagtcatca attacatatc cataactttc
     8881 ttcataagca aaaacaaaat ttaatccgtt atcttcttct ttagcaattt ctctacccat
     8941 tcatttaaat ccagttaaag tttttacaat attaactcca tatttttcat gagcgattct
     9001 atcacccaaa tcacttgtta caaaacttga atatagagcc ggattttttg gaatgctatt
     9061 taagcgtttt agatttgata attttcaatc aattaaaatt ggtcctgttt gatttccatc
     9121 taatcttaca aaatgaccat catgttttat tgccattcca aatctgtcag catctgggtc
     9181 attcataata ataatatctg catcatgttt aataccatat tcaagcggta tttttcatgc
     9241 aggatcaaat tctggatttg gatttacaac atttttaaat gtttcatctt caaatgcatg
     9301 ctcttcaacc tcaataacgt tatatcctga ttcacgtaat atttttgggg taaatttagt
     9361 tcctgttcca ttaactgcgc taaaaataat ttttaaatct tttttagctt cttgctcttt
     9421 tttgtag
//
