LOCUS       sequence-458876-        3029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  medium-copy plasmid for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdO3te
FEATURES             Location/Qualifiers
     source          1..3029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..223
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             271..1221
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELTTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     1222..1238
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1222..1238
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1561..1578)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1561..1577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1698..2903
                     /codon_start=1
                     /gene="tetA from transposon Tn10"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGV
                     LLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLS
                     GITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFI
                     AALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQL
                     IGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLG
                     FIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVS
                     LTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQET
                     SA"
     primer_bind     2904..2923
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2904..2922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3011..3029)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
       61 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
      121 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
      181 acaactcaaa ggaaaaggac atgtaattat cattgactag cccatctcaa ttggtatagt
      241 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
      301 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
      361 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
      421 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
      481 gctaaagcaa ccagagagct gacgacgaga actgtggaaa tcaggaatcc tttggttaaa
      541 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
      601 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
      661 ctggaacatg ttaagtcttt tgaaaacaaa tactccatga ggatttatga gtggttatta
      721 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
      781 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
      841 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
      901 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
      961 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     1021 acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc tttaactgca
     1081 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagcatgat
     1141 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa caacactaga gaacatactg
     1201 gctaaatacg gaaggatctg acaggaaaca gctatgacca tgcccgggta agtgactagc
     1261 ggaaacacag aaaaaagccc gcacctgaca gtgcgggctt tttttttcga ccaaaggctc
     1321 cgaagagccg agtgtcttcg gttcgagacc gaattcgcga gctctcgcta gccagtttaa
     1381 acaattgcgg ccgcgtacta gtctatcgat ttggatccac cctgcaggaa gtctagaccg
     1441 tcgacacggt ctctgagaga agacacgtcg ctcttcgacg taagtaactg actcggtacc
     1501 aaattccaga aaagaggcct cccgaaaggg gggccttttt tcgttttggt cctaagctta
     1561 actggccgtc gttttacata cggccccaag gtccaaacgg tgatagggat aacagggtaa
     1621 tgtaccattt acgttgacac cacctttcgc gtatggcatg acagcgcccg gaagagagtc
     1681 aattcagggt ggtgaatatg aatagttcga caaagatcgc attggtaatt acgttgctcg
     1741 atgccatggg gattggcctt atcatgccag tcttgccaac gttattacgt gaatttattg
     1801 cttcggaaga tatcgctaac cactttggcg tattgcttgc actttatgcg ttaatgcagg
     1861 ttatctttgc tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt
     1921 tgttgtcatt aataggcgca tcgctggatt acttattgct ggctttttca agtgcgcttt
     1981 ggatgctgta tttaggccgt ttgctttcag ggatcacagg agctactggg gctgtcgcgg
     2041 catcggtcat tgccgatacc acctcagctt ctcaacgcgt gaagtggttc ggttggttag
     2101 gggcaagttt tgggcttggt ttaatagcgg ggcctattat tggtggtttt gcaggagaga
     2161 tttcaccgca tagtcccttt tttatcgctg cgttgctaaa tattgtcact ttccttgtgg
     2221 ttatgttttg gttccgtgaa accaaaaata cacgtgataa tacagatacc gaagtagggg
     2281 ttgagacgca atcaaattcg gtgtacatca ctttatttaa aacgatgccc attttgttga
     2341 ttatttattt ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg gtgctattta
     2401 ccgaaaatcg ttttggatgg aatagcatga tggttggctt ttcattagcg ggtcttggtc
     2461 ttttacactc agtattccaa gcctttgtgg caggaagaat agccactaaa tggggcgaaa
     2521 aaacggcagt actgctcgga tttattgcag atagtagtgc atttgccttt ttagcgttta
     2581 tatctgaagg ttggttagtt ttccctgttt taattttatt ggctggtggt gggatcgctt
     2641 tacctgcatt acagggagtg atgtctatcc aaacaaagag tcatcagcaa ggtgctttac
     2701 agggattatt ggtgagcctt accaatgcaa ccggtgttat tggcccatta ctgtttgctg
     2761 ttatttataa tcattcacta ccaatttggg atggctggat ttggattatt ggtttagcgt
     2821 tttactgtat tattatcctg ctatcaatga ccttcatgtt gacccctcaa gctcagggga
     2881 gtaaacagga gacaagtgct tagtaatacg actcactata gggtaactaa ttgaggcgcg
     2941 cctaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc aaccttccta
     3001 ggtaagtgac cctttagtga gggttaatt
//
