LOCUS       sequence-458877-        2009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  medium-copy plasmid for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdO3t
FEATURES             Location/Qualifiers
     source          1..2009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..223
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             271..1221
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELTTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     1222..1238
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1222..1238
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1561..1578)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1561..1577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1581..1628
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1647..1883
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     primer_bind     1884..1903
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1884..1902
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1991..2009)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
       61 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
      121 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
      181 acaactcaaa ggaaaaggac atgtaattat cattgactag cccatctcaa ttggtatagt
      241 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
      301 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
      361 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
      421 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
      481 gctaaagcaa ccagagagct gacgacgaga actgtggaaa tcaggaatcc tttggttaaa
      541 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
      601 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
      661 ctggaacatg ttaagtcttt tgaaaacaaa tactccatga ggatttatga gtggttatta
      721 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
      781 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
      841 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
      901 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
      961 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     1021 acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc tttaactgca
     1081 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagcatgat
     1141 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa caacactaga gaacatactg
     1201 gctaaatacg gaaggatctg acaggaaaca gctatgacca tgcccgggta agtgactagc
     1261 ggaaacacag aaaaaagccc gcacctgaca gtgcgggctt tttttttcga ccaaaggctc
     1321 cgaagagccg agtgtcttcg gttcgagacc gaattcgcga gctctcgcta gccagtttaa
     1381 acaattgcgg ccgcgtacta gtctatcgat ttggatccac cctgcaggaa gtctagaccg
     1441 tcgacacggt ctctgagaga agacacgtcg ctcttcgacg taagtaactg actcggtacc
     1501 aaattccaga aaagaggcct cccgaaaggg gggccttttt tcgttttggt cctaagctta
     1561 actggccgtc gttttacact gttgacaatt aatcatcggc atagtatatc ggcatagtat
     1621 aatacgacaa ggtgaggaac taaaccatgg gtcaaagtag cgatgaagcc aacgctcccg
     1681 ttgcagggca gtttgcgctt cccctgagtg ccacctttgg cttaggggat cgcgtacgca
     1741 agaaatctgg tgccgcttgg cagggtcaag tcgtcggttg gtattgcaca aaactcactc
     1801 ctgaaggcta tgcggtcgag tccgaatccc acccaggctc agtgcaaatt tatcctgtgg
     1861 ctgcacttga acgtgtggcc taataatacg actcactata gggtaactaa ttgaggcgcg
     1921 cctaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc aaccttccta
     1981 ggtaagtgac cctttagtga gggttaatt
//
