LOCUS       sequence-458885-        8183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing human STX3 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8183)
  TITLE     Lentiviral plasmids expressing popular genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti puro HA-STX3
FEATURES             Location/Qualifiers
     source          1..8183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    181..306
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             353..533
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(534..760)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(788..808)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(788..806)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(857..873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(857..873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(870..892)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    881..897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(905..911,912..929,930..935))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    950..971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1088..1105)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1259..1847)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1339..1358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2018..2809,2810..2878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2641..2660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2879..2983)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3051..3069
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3107..3129)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3229..3248
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3442..3464
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3456..3473
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3457..3473
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3505..3524
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3505..3523
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3544..3679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3719..3840
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3730..3749)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3784..3803
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3912..4145
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4273..4872)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4356..4376)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4853..4872
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4894..5404)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4957..4975)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    5453..5570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5925..5936)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(6525..6551)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(6599..6802)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6628..6648)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(7091..7111)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(7409..7453)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7638..7871
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
       61 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
      121 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
      181 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
      241 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
      301 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
      361 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
      421 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
      481 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccgtttatg
      541 tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca
      601 atccatgtca gacccgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc
      661 accaatcggc atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt
      721 accatgttgc aagactacaa gagtattgca taagactaca ttaagcttgc agctccagct
      781 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatgctgc aggcatgcaa
      841 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      961 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1021 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1081 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1141 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1201 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1261 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1321 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1381 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1441 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1501 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1561 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1621 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1681 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1741 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1801 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1861 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1921 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1981 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2041 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2101 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2161 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2221 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2281 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2341 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2401 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2461 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2521 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2581 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2641 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2701 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2761 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2821 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2881 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2941 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3001 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3061 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     3121 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     3181 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     3241 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     3301 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     3361 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     3421 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg
     3481 agctcggtac ccggggagcg cgcgtaatac gactcactat agggcgaatt gggtacgtcc
     3541 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     3601 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     3661 gcggagttag gggcgggatg ggcggagtta ggggcgggat agctagagcc agacatgata
     3721 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     3781 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3841 cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca
     3901 tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     3961 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     4021 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     4081 ccagagagac ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc
     4141 ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     4201 tggtcttaaa ggtaccgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc
     4261 gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg
     4321 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg
     4381 cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag
     4441 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag
     4501 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc
     4561 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
     4621 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca
     4681 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
     4741 ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
     4801 ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt
     4861 gtactcggtc atggtaagct ccggtggatc cccctgggga gagaggtcgg tgattcggtc
     4921 aacgagggag ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc
     4981 gggcaggaac agggcccaca ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg
     5041 gccgctgctc tcggcgcgcc ctgctgagca gccgctattg gccacagccc atcgcggtcg
     5101 gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc
     5161 ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg
     5221 agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg aagatccggg tgacgctgcg
     5281 aacggacgtg aagaatgtgc gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg
     5341 cccagagcag ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa
     5401 ccccctcgag ccgcggccaa agtggatctc tgctgtccct gtaataaacc cgaaaatttt
     5461 gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta
     5521 ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga
     5581 atactgccat ttgtctcgag gtcgagaatt ctctagagaa gcttgagctc gagatctgag
     5641 tccggctaat tgagtccaac agagaggccg ataatgaggg cgagaatacc gaggagaacc
     5701 acaaccaaca ctattatgat gatcagcttc ttccgtgcct gtgactgata tttaacggct
     5761 tttttggttt cgtcgcgtgc tttttcaacg tggtctaccg tatgcattac attcaactca
     5821 atgttgtcga gcatttcgcc ctgattctcc acgagcatgg caatgtccat gaacatgtcg
     5881 tgaagctcct ttatactgct ttccaggcgc actatgtcct tgtgcctccc ttcgatctcg
     5941 ctcaatgctt gtttgctgat ttgactgtct attataccac ttgtaaaaat tgcgggattg
     6001 ccggattcca acatttcctc caactcctcg tctgttgttt tctttccagt aatttccaat
     6061 tgcctctgta tcctaccttt agatcgctcg cgaaagtcga cctgtgcctc gttgtatttc
     6121 gtcatcacct ccacaaattt gcgagagagg acgctatgct gggactttcg tattcggagg
     6181 tcggcagaag accggacctc gtcttcttct atatgtttct ccatactttt cagtttgttc
     6241 ctgacattat tcgccctctt ctttatttct gtcgtgagct gttccaaatc atccttcgtc
     6301 ttgggttcag gtatgggagc actcaatatg atggaataca gtttcttcgc ctcctcaacg
     6361 tgttcagaaa tcttatcgat gttgagccgc gtttcctcaa tctctgaaaa aaattcgtcc
     6421 atgaacgcgg tgttatcaat agcgatctcc actgcatctg tgtcatcatc ttgcgtcaac
     6481 tgcttagctt tcagttgttc caggcgatct ttcatggatc cgctagcgta atccggaaca
     6541 tcgtatgggt acatggtggc gaccggtagc gctagcggat ctgacggttc actaaaccag
     6601 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     6661 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     6721 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     6781 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     6841 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     6901 cattgacgtc aatagggggc gtacttggca tatgatagtc cattttaaaa cataatttta
     6961 aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct
     7021 ttgtgtttac agtcaaatta attccaatta tctctctaac agccttgtat cgtatatgca
     7081 aatatgaagg aatcatggga aataggccct cggtgaaggg ggcggccgct cgaggctagt
     7141 ctcgtgatcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct
     7201 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg
     7261 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     7321 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     7381 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     7441 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     7501 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     7561 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     7621 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     7681 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     7741 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     7801 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     7861 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     7921 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     7981 aattgtccct catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg
     8041 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     8101 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     8161 tctgatcctg tctgaaggga tgg
//
