LOCUS       sequence-459042-       14493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the production of SARS-CoV-2 virus-like particles (VLPs) in the 
            '2-plasmid' system and packaging EGFP mRNA into the VLPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14493)
  AUTHORS   Yang Q, Kelkar A, Manicassamy B, Neelamegham S
  TITLE     Conserved role of spike S2 domain N-glycosylation across 
            betacoronaviruses.
  JOURNAL   Npj Viruses. 2025 Jan 25;3(1):4. doi: 10.1038/s44298-024-00085-7.
  PUBMED    40295734
REFERENCE   2  (bases 1 to 14493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Npj 
            Viruses."; date: "2025-01-25"; volume: "3(1):4. doi"; pages: " 
            10.1038/s44298-024-00085-7"
COMMENT     Sequence Label: LVDP 2P.2.EGFP
FEATURES             Location/Qualifiers
     source          1..14493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2666..3254
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2772..2783
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3181..3201
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     misc_feature    join(3525..3533,3534..3536,3537..4111)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3698..3717)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     3927..3944
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(4789..4842)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        complement(6145..6348)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        6349..6652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7584..7632
                     /note="synthetic polyadenylation signal"
     CDS             complement(join(8809..9525,9526..9528,9529..9531))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKG"
     primer_bind     complement(8857..8878)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9206..9225
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9465..9486
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9528..9537
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9544..9755)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             10295..10475
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10501..10518)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10507..10731
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10777..11205
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10864..10883)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11074..11095
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11214..11234)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11219..11548
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11399..11534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11461..11480
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11596..11643
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11662..12036
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12166..12287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12203..12222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12257..12276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12336..12352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12336..12352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12349..12371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12360..12376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12384..12390,12391..12408,12409..12414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12429..12450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12567..12584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12738..13326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12818..12837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13497..14288,14289..14357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14120..14139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14358..14462)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcatc tttaatttct
     2401 aatatattta gaatctttaa tttctaatat atttagaatc tttaatttct aatatattta
     2461 gaatctttaa tttctaatat atttagaatc tttaatttct aatatattta gaatctttaa
     2521 tttctaatat atttagaatc tttaatttct aatatattta gaatctttaa tttctaatat
     2581 atttagaagg atctccgcgt ttatgaacaa acgacccaac acccgtgggt tttttctgtc
     2641 tttttattgc cgtcattctc gaggggcggg gaggcggccc aaagggagat ccgactcgtc
     2701 tgagggcgaa ggcgaagatg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga
     2761 aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag
     2821 gtggcaacac aggcgagcag ccatggaaag gacgtcagct tccccgacaa caccacggaa
     2881 ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt
     2941 tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg
     3001 gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc
     3061 ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag
     3121 aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca
     3181 catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa
     3241 tccagaggtt gattgtcgag tcggtcgagt taaagaacga ggagatccgg caccctgcta
     3301 gaattgagat tcttcaccct gctgtaaacg tagaatgagg gtttcacgag agatacattg
     3361 acaatgttac aacagtaggc gcaaagcctc aatgcagtca gaattgccag tgtgacgagg
     3421 agaaatacga cgaatgccag aaacaagagc acagaattta cgatcagtgt cccggtttct
     3481 tctgatacga agctgtacat ggtggcggcg aatttctcct tgtgggttgt ggcaagctta
     3541 tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt
     3601 taacctcgac taaacacatg taaagcatgt gcaccgaggc cccagatcag atcccataca
     3661 atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg agagccattt
     3721 gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg cctttgcagg
     3781 tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg gggggttccg
     3841 ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc
     3901 ttccttcacg acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg
     3961 aatgctcgtc aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa
     4021 tatggtggaa aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc
     4081 agtaacgtta gggggggggg agggagaggg gcgggatcct tactggacga gcaaagcaat
     4141 gttatcagaa gatgatgagt ggtctgtatt cagtttatag ttccctatcc tgtagcgtga
     4201 ataggctgca aatccactat cgccagcgac cctttgggac gctccaagct tgtaatagct
     4261 caaagttcga ctggtagcta cagttatttc ttttggcaaa tccttaatat cacagcgacc
     4321 aagatgatgt cctgcgatcc tcaagtggcc cctcaggatc acggctccaa tcaccaattc
     4381 tgattcaagg agcggtcggg tcaaaattgt gccatgcagg gggacattaa gcaagatatt
     4441 tgtttcaggg ttaaatgacc acatgcttcg cgtgcgtgca aaaagtctga atgacgctat
     4501 aaaataggag agccacatca aacctaccag gcatgccata gctattgcta tgccgccagt
     4561 tatccagttt attcgataca ccgccgcgag tacgaagcag gcaagggtca caggccacag
     4621 gagccaaagg aatataagtt ttataatata gaggaatcgg ttgcgattcg cgtatgcgaa
     4681 ttgcaggaga caaatccatg tcaagaagag aaatccaatc acaaggttcc attgctccaa
     4741 cagttttttc agctcctcta cagtaatggt cccatttgag tcggccattg ggccaggatt
     4801 ctcctccacg tcaccgcatg ttaggagact tcctctgccc tctcctccgg acccctgggt
     4861 gctatcggcg ctagacatag actgctggag ctgcttggaa aaatcgtcca ggtcggcggc
     4921 tggcagcagt gtcacggtct gctgcttctt ctgtctctga ggcagggcct gggtctcatc
     4981 ggccttcttc ttcttgtcct tcttgggctc tgtaggggga aaggtcttat aggcatcgat
     5041 gtgcttgttc agcaggatga cctgatcctt gaagttaggg tccttatcgt ccagcttgat
     5101 ggcgcctgtg taggtcagcc atgtgccgga tggggtcacc tccattccga tcctgctcat
     5161 gccaaagaag gcggaggcgc ttggtgcgaa ctgtgcgatc tgaggccagt gcttataatc
     5221 ggtgccctgc ctgatcagct cctggtcgcc aaaattgccc tgtgtctgct ctggtccgcg
     5281 cctgccgaag gcctgggtca cgttgtaggc ctttgtggcg gtgcgcttct gccttggctt
     5341 cttgctggcc tcggcggcag acttctttgt cacggtctgt ccctgctgct gctgtccctt
     5401 gccgctcatc ttagactcca gctgattcag tctatccagc agcagcaggg ccagggcggc
     5461 gtcgcctccg tttcctgcca ttcttgcagg gcttgtgccc atggagctgc ctggggtaga
     5521 attccgagag gagtttctgg accggctaga ggagcggcta gaggcctggg agccgcccct
     5581 gctgccctct gcgtaaaagc ccttgggcag tgtggtgccc tgtggcagct ggagcacgat
     5641 tgctgcattg ttggcgggat tgcgtgtgcc gatgtgatcc tttggtgtgt tcagggcgcc
     5701 ctcggttgcc acccagatga ttccgtcctt attggcgcca tatggcagtc ctgcctcagg
     5761 tccggtgccc agatagtaga agtaccatct gggggacaga tccttcatct tgccgtcgcc
     5821 tccccggatt ctccttgttg ccctcctata gtagccgatc tgatcgtcag gggagctatt
     5881 ggtgttgata ggcacgccct ggcccctggg aaacttcagg tcctccttgc cgtgctgtgt
     5941 cagggcggtg aaccagctgg cggtattgtt gggcaggccc tgtggtctcc gctgcttaga
     6001 gcgtgctccg gacctctctc cattctggtt ggagcctgta gaatcggagg ggccgccgaa
     6061 ggtgattctt ggggcattcc gctggttctg agggccgttg tcagacatgg tggcgagcta
     6121 gcggatctga cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct
     6181 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
     6241 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
     6301 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
     6361 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
     6421 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
     6481 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     6541 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     6601 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
     6661 catatatggg ctatgaacta atgaccccgt aattgattac tattaatcga atttgtggat
     6721 ccgtgacgca ctgaacaggt tgcccaagga ggctgtggat gccccatccc tgcaggcatt
     6781 caaggccagg ctggatgtgg ctctgggcag cctgggctgc tggttggcga ccctgcacat
     6841 agcagggggt tggagctggg tgagcactgt gctcctttgc aacccaggcc attctatgat
     6901 tctgtcattc taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc
     6961 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcgcgtgc caccttcccc
     7021 gtgccgggct gtccccgcac gctgccggct ccggctcggg gatgcggggg gagcgccgga
     7081 ccggagcgga gccccgggcg gctcgctgct gccccctagc ggaagaggga tgcaattaca
     7141 tccctggggg ctttggcggg gggctctccc ttaattaact gacagccacg atcccatagg
     7201 tgaaggcaaa ggcgtggcag ctaggaacac tcactacacc cccactcaag ggaacaccgt
     7261 gcccagctac cctgagacag ccccactccc aggaaatgca ggtgccatgc ctcactgggg
     7321 ctggttggca cctgagccag acacccccaa atagtccctt cccacctcct caagcatctg
     7381 cactctgggt gaacccagaa aaactggggt acctggggag acatagaagg gccaggccag
     7441 gaactcccca ataagcagga acagagacct gacccctgag cctcccccac cctctaaggc
     7501 tgctcaatgt caaggcagga gggatctcca cacacctgca ctctgggtaa ggacaagttg
     7561 gcccccactg ccccacctct agacacacaa aaaaccaaca cacagatcta atgaaaataa
     7621 agatctttta tttctagcag gcgatcgcct tatggagttg ggatctatct ttatagccac
     7681 ggaacctcca agagctagct tttgttgtat aaacccacaa atgtaagtga aaaaaccctc
     7741 tttagagtca ttttcttttg taacattttt agtcttaggg tcgtacatat cactaataat
     7801 gagatcccat ttattagctg tatgtacagt tgcacaatca ccaatcaaag ttgaatctgc
     7861 atcagagaca aagtcattaa gatctgaatc gacaagcagc gtacccgtag gcaaccactg
     7921 tcttaaaaca gctgtacctg gtgcaactcc tttatcagaa ccagcaccaa aatgtataac
     7981 tctcatatta tagggtacag ctaatgttaa tgtgtttaaa tattgacaca gttgagtata
     8041 ttttgcgaca ttcatcatta tgcctttagg taatgttgca ctatcaccat aattttgaag
     8101 gtcacacttt tctaatagca ttctttgcat tttgtaaaga ttaggcatag caacacccgg
     8161 ttgccacgct tgactagatt gtaattttgg gtaaaatgtt tctacatggc catctttaca
     8221 ccaaagcata aatgaaattt ctgtatagtc aatagtcact ttgacaacct tagaaactac
     8281 agataaatct tgggatttta ttatttcaac aaaatcatca agtaataaat caataacaga
     8341 acacacacac ttagatgaac ctgtttgcgc atctgttatg aaatagtttt taactgtact
     8401 gtccatagga ataaaatctt ctaattcaaa aggtgattcc ttaaaacgtt tagctagtcc
     8461 aatcagtaga tgtaaaccac ctaactgact atgactaaaa tctccataaa cgatatgttc
     8521 gaaggcatag ccttctaatt tataccgttc aatgaattca tccatagcta attctaagaa
     8581 atcaatttcc atttgactcc tgggtttaaa ttcttgtaaa tttctactct gagtaaagta
     8641 agtttcaggt aattgttgga caacaccatc aactttctta taataattga actgtgtttt
     8701 tacggcttct ccaattaatg tgactccatt aagactagct tgtttgggac ctacagagat
     8761 ctatatctcc ttcttaaagg aggcggccgc ataagcgaat tcttgatttt atcccttgta
     8821 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     8881 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
     8941 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     9001 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     9061 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     9121 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     9181 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     9241 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     9301 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
     9361 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     9421 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     9481 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgac
     9541 cggctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga ctactgcact
     9601 tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga catcactttc
     9661 ccagtttacc ccgcgccacc ttctctaggc accggatcaa ttgccgaccc ctccccccaa
     9721 cttctcgggg actgtgggcg atgtgcgctc tgcccacgtg gaattatcga ttccgcggtg
     9781 aattcttcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     9841 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     9901 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     9961 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
    10021 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
    10081 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
    10141 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
    10201 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
    10261 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
    10321 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10381 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10441 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
    10501 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
    10561 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
    10621 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
    10681 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
    10741 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
    10801 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
    10861 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
    10921 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
    10981 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
    11041 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
    11101 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
    11161 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
    11221 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    11281 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
    11341 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
    11401 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    11461 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
    11521 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
    11581 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
    11641 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
    11701 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
    11761 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
    11821 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
    11881 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
    11941 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
    12001 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
    12061 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
    12121 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
    12181 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    12241 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
    12301 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    12361 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    12421 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    12481 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    12541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    12601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    12661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    12721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    12781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    12841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    12901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    12961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    13021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    13081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    13141 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    13201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    13261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    13321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    13381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    13441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    13501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    13561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    13621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    13681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    13741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    13801 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    13861 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    13921 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    13981 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    14041 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    14101 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    14161 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    14221 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    14281 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    14341 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    14401 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    14461 cgcacatttc cccgaaaagt gccacctgac gtc
//
