LOCUS       sequence-459062-       12493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Seeling JM, Miller JR, Gil R, Moon RT, White R, Virshup DM
  TITLE     Regulation of beta-catenin signaling by the B56 subunit of protein 
            phosphatase 2A.
  JOURNAL   Science. 1999 Mar 26. 283(5410):2089-91.
  PUBMED    10092233
REFERENCE   2  (bases 1 to 12493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1999 Mar 26. 283(5410):2089-91."
COMMENT     Sequence Label: V246 pCEP 4HA B56beta
FEATURES             Location/Qualifiers
     source          1..12493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        57..436
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        437..640
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     591..611
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     637..661
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             694..720
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             736..825
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(2998..3014)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2999..3015)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3030..3164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3054..3073)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3108..3127
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3307..3329)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3889..5678
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5980..7905)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(8340..8358)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8426..8530
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8531..8599,8600..9391)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8749..8768)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9562..10150
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10051..10070
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10581..10726
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10769..11806
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11804..11823)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11848..11896
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttgaca
       61 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      121 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      181 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      241 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      301 gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      361 ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt
      421 catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt
      481 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      541 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg
      601 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
      661 ctctagaagc tgggtaccac gcgtcatatg gcttacccat acgatgttcc agattacgct
      721 agcggccgca tcttttaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc
      781 ccggactatg caggatccta tccatatgac gttccagatt acgctgctca gtgcggccgc
      841 atggagacga agctgccccc tgcaagcacc cccactagcc cctcctcccc cgggctgtcg
      901 cctgtgcccc cacccgacaa ggtggacggc ttctcccgcc gttccctccg cagagcccgg
      961 ccccgccgct cccacagctc ctctcagttc cgctatcaga gcaaccagca agagctcaca
     1021 ccgctgcccc tgctcaaaga tgtgccggct tccgagctgc acgagctgct gagccggaag
     1081 ctggcccagt gtggggtgat gtttgacttc ttggactgtg tggccgacct caaggggaag
     1141 gaggtgaagc gggcagccct caacgagctg gtggagtgtg tggggagcac ccggggtgtc
     1201 ctcatcgagc ccgtctaccc agacatcatc cgcatgatct cagtgaatat cttccggact
     1261 ctgccgccca gtgagaaccc tgaatttgac cctgaagagg atgagcccaa tcttgagcct
     1321 tcgtggccac acctgcagct ggtatatgag tttttcctgc gtttcttgga gagcccagac
     1381 ttccagccct ccgtggccaa gagatatgtg gatcaaaagt ttgtcctgat gctcctggag
     1441 ctatttgata gtgaggatcc ccgggagcgt gagtacctca agaccatcct gcaccgggtc
     1501 tatggcaagt tcctgggtct ccgggcctac atccgcaaac agtgcaacca catcttcctc
     1561 cggttcatct atgaattcga gcacttcaat ggtgtggctg agctgctgga gatcctagga
     1621 agcatcatca atggctttgc gctgcccctg aagacggagc acaagcagtt cctggttcgc
     1681 gtcctgatcc ccctgcactc tgtcaagtcg ctgtctgtct tccatgccca gctggcatac
     1741 tgtgtggtgc agttcctgga gaaggatgcc actctgacag agcacgtgat ccgggggctg
     1801 ctcaaatact ggccaaaaac ctgcacccag aaggaggtga tgtttctggg ggagatggaa
     1861 gagattcttg atgtcgtcga gccctcccag tttgtgaaga tccaggagcc cctttttaag
     1921 caggtggctc gctgtgtttc cagcccccat ttccaggttg cagagcgggc tctgtatttc
     1981 tggaacaatg agtatatcct aagcctcatt gaggacaact gccacactgt gctgcctgct
     2041 gtgtttggga ccctctacca agtctccaag gagcactgga accaaaccat cgtatcactg
     2101 atctacaatg tgctcaagac cttcatggag atgaatggga agctgtttga tgagctcaca
     2161 gcctcctaca agctggaaaa gcagcaggag cagcagaagg cccaggagcg tcaggagtta
     2221 tggcaaggtc tggaggagct gcggctacgc cggctacagg ggacccaggg ggccaaggag
     2281 gcccccctcc agcggcttac accccaggtg gccgccagtg ggggtcagag ctagacagca
     2341 cctcagaagg ggaaaagcta aacccagagc tgtcagtccc tctatccctt ctcctgtcca
     2401 ggggcccaga gagaaacaca cctaccccgg gccttgccag agtggcttct gaggactccc
     2461 tgcccagccc agctttcact ggggggagac gaggagaggc aatggtggtc ttggcaacag
     2521 aatgctcagc ccctcgtggc aggacttgac aagggcaagc ttgaccagga agctgccatc
     2581 agggatcttc ccctgccccg caaagctagg ctccagctgc aggcgggctc ccaccctctg
     2641 ctcctggcct tgggcaaggg cactcagcgc ctcgcctgcc cctgccttgg ccaatgcgag
     2701 gtccttcctt atccccacca tggggtccat ggtctattta ttctcgccca gctcaccctc
     2761 tacacagacg ctgtcctggg tgcacactcc tcccttccct cgctgtgtac ttccttgtcc
     2821 cctttttatt tattgggcag ggggaggggg agggcacagg caagaagaga ttcacagtgt
     2881 cctggggtaa gggggggttc acagtaatca tggtctactc ctctttccgt ggctgggggt
     2941 agacttaata aagagagaaa ttcaaaaaaa aaaaaaggaa ttcgatatca agcttatcga
     3001 taccgtcgac ctcgaggccg gcaaggccgg atccagacat gataagatac attgatgagt
     3061 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3121 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3181 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     3241 tctacaaatg tggtatggct gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg
     3301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     3361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     3421 catgaggtcg actctagagg atcgatcccc gccccggacg aactaaacct gactacgaca
     3481 tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg gtacaacttg
     3541 ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag gggttctctg
     3601 actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag tggctttcat
     3661 cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat gtgtaactct
     3721 tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc caggaagact
     3781 acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata agcggaccct
     3841 caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa cgggtagcat
     3901 atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata gcatatgtta
     3961 cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa ggaacagcga
     4021 tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat tccacgaggg
     4081 tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg cagtgaactc
     4141 tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat aactgctgag
     4201 ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag
     4261 aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa tataaccctc
     4321 acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat atctttaaca
     4381 atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc acacgaattt
     4441 atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt aagatgtgtc
     4501 ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa caaggggaaa
     4561 gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc cgaaaattaa
     4621 acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct tttttttgaa
     4681 attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa
     4741 ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc aaaccacttg
     4801 cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg gcgggccaag
     4861 ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc ctgagcgcca
     4921 agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg ggctaatgtt
     4981 gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     5041 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5101 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     5161 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     5221 cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct aatttatatc
     5281 tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg
     5341 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     5401 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     5461 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     5521 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg catatacagt
     5581 cagcatatga tacccagtag tagagtggga gtgctatcct ttgcatatgc cgccacctcc
     5641 caagggggcg tgaattttcg ctgcttgtcc ttttcctgct ggttgctccc attcttaggt
     5701 gaatttaagg aggccaggct aaagccgtcg catgtctgat tgctcaccag gtaaatgtcg
     5761 ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct gagtgacgtg acaacatggg
     5821 tatgcccaat tgccccatgt tgggaggacg aaaatggtga caagacagat ggccagaaat
     5881 acaccaacag cacgcatgat gtctactggg gatttattct ttagtgcggg ggaatacacg
     5941 gcttttaata cgattgaggg cgtctcctaa caagttacat cactcctgcc cttcctcacc
     6001 ctcatctcca tcacctcctt catctccgtc atctccgtca tcaccctccg cggcagcccc
     6061 ttccaccata ggtggaaacc agggaggcaa atctactcca tcgtcaaagc tgcacacagt
     6121 caccctgata ttgcaggtag gagcgggctt tgtcataaca aggtccttaa tcgcatcctt
     6181 caaaacctca gcaaatatat gagtttgtaa aaagaccatg aaataacaga caatggactc
     6241 ccttagcggg ccaggttgtg ggccgggtcc aggggccatt ccaaagggga gacgactcaa
     6301 tggtgtaaga cgacattgtg gaatagcaag ggcagttcct cgccttaggt tgtaaaggga
     6361 ggtcttacta cctccatata cgaacacacc ggcgacccaa gttccttcgt cggtagtcct
     6421 ttctacgtga ctcctagcca ggagagctct taaaccttct gcaatgttct caaatttcgg
     6481 gttggaacct ccttgaccac gatgctttcc aaaccaccct ccttttttgc gcctgcctcc
     6541 atcaccctga ccccggggtc cagtgcttgg gccttctcct gggtcatctg cggggccctg
     6601 ctctatcgct cccgggggca cgtcaggctc accatctggg ccaccttctt ggtggtattc
     6661 aaaataatcg gcttccccta cagggtggaa aaatggcctt ctacctggag ggggcctgcg
     6721 cggtggagac ccggatgatg atgactgact actgggactc ctgggcctct tttctccacg
     6781 tccacgacct ctccccctgg ctctttcacg acttcccccc ctggctcttt cacgtcctct
     6841 accccggcgg cctccactac ctcctcgacc ccggcctcca ctacctcctc gaccccggcc
     6901 tccactgcct cctcgacccc ggcctccacc tcctgctcct gcccctcctg ctcctgcccc
     6961 tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc
     7021 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     7081 tgcccctcct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc
     7141 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc
     7201 tgcccctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     7261 tgctcctgcc cctcctgccc ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc
     7321 ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc
     7381 tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     7441 tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc
     7501 ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc
     7561 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc
     7621 cgctcctgct cctgctcctg ttccaccgtg ggtccctttg cagccaatgc aacttggacg
     7681 tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc cgcccggggc
     7741 tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc
     7801 ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt ctcccttctc
     7861 tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc acactaaaag
     7921 agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc agactcctgc
     7981 cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac agatccaggt
     8041 ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact cgcagcccgg
     8101 aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt
     8161 cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct
     8221 cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct
     8281 gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg
     8341 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     8401 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8461 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8521 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8581 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8641 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8701 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8761 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8821 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8881 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8941 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9001 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9061 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9121 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9181 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9241 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9301 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9361 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9421 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9481 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9541 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9601 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9661 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     9721 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9781 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9841 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9901 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9961 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10021 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10081 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10141 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg
    10201 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
    10261 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
    10321 gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg
    10381 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
    10441 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccct
    10501 gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt
    10561 tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt
    10621 cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat attaaggtga
    10681 cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg
    10741 tgccgcagat cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt
    10801 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
    10861 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
    10921 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
    10981 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
    11041 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
    11101 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
    11161 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
    11221 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
    11281 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
    11341 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
    11401 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
    11461 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
    11521 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
    11581 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
    11641 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
    11701 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
    11761 acgccccagc actcgtccgg atcgggagat gggggaggct aactgaaaca cggaaggaga
    11821 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
    11881 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
    11941 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
    12001 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
    12061 gccactggcc ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg
    12121 gggttattat tttgggcgtt gcgtggggtc aggtccacga ctggactgag cagacagacc
    12181 catggttttt ggatggcctg ggcatggacc gcatgtactg gcgcgacacg aacaccgggc
    12241 gtctgtggct gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc
    12301 aagctagtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
    12361 tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt
    12421 ggcaattagc catattagtc attggttata tagcataaat caatattggc tattggccat
    12481 tgcatacgtt gta
//
