LOCUS       sequence-459122-        5417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase vector containing the hs737 enhancer sequence (sequence 
            containing variant 1). Variant 1 is chr10:128568698-A-G where the 
            information is chromosome:positionInB38-RefAllele-AltAllele..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Padhi EM, Hayeck TJ, Cheng Z, Chatterjee S, Mannion BJ, 
            Byrska-Bishop M, Willems M, Pinson L, Redon S, Benech C, Uguen K, 
            Audebert-Bellanger S, Le Marechal C, Ferec C, Efthymiou S, Rahman F,
            Maqbool S, Maroofian R, Houlden H, Musunuri R, Narzisi G, Abhyankar 
            A, Hunter RD, Akiyama J, Fries LE, Ng JK, Mehinovic E, Stong N, 
            Allen AS, Dickel DE, Bernier RA, Gorkin DU, Pennacchio LA, Zody MC, 
            Turner TN
  TITLE     Coding and noncoding variants in EBF3 are involved in HADDS and 
            simplex autism.
  JOURNAL   Hum Genomics. 2021 Jul 13;15(1):44. doi: 10.1186/s40246-021-00342-3.
  PUBMED    34256850
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum 
            Genomics."; date: "2021-07-13"; volume: "15(1):44. doi"; pages: " 
            10.1186/s40246-021-00342-3"
COMMENT     Sequence Label: pGL4.23-hs737.1
FEATURES             Location/Qualifiers
     source          1..5417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1211..1242
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1269..1278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1275..2927
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2971..3092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2982..3001)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3036..3055
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3340..3357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3511..4099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3591..3610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4299..5090,5091..5159))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5264..5312
                     /note="synthetic polyadenylation signal"
     misc_feature    5326..5417
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5366..5385
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tgacaatgcg ctggaaaatc
       61 tccgcggatg tggaggccgt gtttggtttt ctctgtgtgt gtgttttctc taaagccata
      121 agtgcgcaac agtgaaatct tacttgcaga gatccccatt atgcatcggt cccctgaagc
      181 tgctgagatg acaccacctc ttacaggtgg cagagagaat gcggcaaacc cctgaagtct
      241 caagtcacag caatcatctt caactcccaa attgtcctgg aaggagagtg gcatcggatt
      301 tgtcttaggc tgtttggcca aactggggca cagatgggat aatgactttt acaaacagca
      361 ttttgatgag aggaggggag aaggaatcat atgtaaaatg cacaaaggct gcataaacac
      421 actgatgcct gcagctggaa caaactaaaa agatgtttaa taatttagaa ccaagcaggc
      481 agggcctgtg cagctgttgt gtgcagggga ccatgaaagt cttccatttg acagctggcc
      541 ccatctttcc ccagttgcta cgtgaagaag agaatgcaga tctgggatta gccaatccct
      601 gttacccttg cccatctgcc accaagaccc ccagctctgc ctccctcgca gaaaggcgca
      661 cacataaaga aggctggcct tcccaagcat atgcatgcgt cgctattagc cgtcagggcc
      721 gcccgctccc aggatctgtt gacacgccgc cagtacatgg ctgctgtcgt ttttgctgga
      781 gggcacattt tgcatttcac acatttgcag aaatacaaaa tgctgttgga tgcaaacaat
      841 tcagggccta attggctgaa ttacttcaat gttaccttgt gtcccccacc tgattaacat
      901 agctcagctg ggagggctgg ggaaggagga ggagagaggg atttggaggg caggtgaagc
      961 tgtgtttgcc caggaaagcg agatccttag cgaagtccca gctgagtgtg gcctgtgcca
     1021 agcagatagc tgagtgcggg aggggccgtg caggctgcat tcttccacag gacgcccgga
     1081 ggagggggct ctgtggagga acaataaaat ttcagggcac agaggaggca ggctgtcggg
     1141 gaggagcggg gggcagtgcc ccttttgtga gatgtatcaa gatctggcct cggcggccaa
     1201 gcttagacac tagagggtat ataatggaag ctcgacttcc agcttggcaa tccggtactg
     1261 ttggtaaagc caccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc
     1321 cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg
     1381 tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt
     1441 acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc
     1501 atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc
     1561 tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga
     1621 acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga
     1681 tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga
     1741 ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct
     1801 tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca
     1861 tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt
     1921 gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg
     1981 ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact
     2041 tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca
     2101 gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg
     2161 ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg
     2221 gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca
     2281 tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg
     2341 acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact
     2401 tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca
     2461 tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg
     2521 gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg
     2581 accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga
     2641 gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg
     2701 atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga
     2761 aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg
     2821 ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc
     2881 gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgtaataa ttctagagtc
     2941 ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     3001 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     3061 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     3121 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     3181 gtaaaatcga taaggatccg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     3241 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     3301 caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg
     3361 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3421 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4141 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4201 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4261 gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt accagtgctt gatcagtgag
     4321 gcaccgatct cagcgatctg cctatttcgt tcgtccatag tggcctgact ccccgtcgtg
     4381 tagatcacta cgattcgtga gggcttacca tcaggcccca gcgcagcaat gatgccgcga
     4441 gagccgcgtt caccggcccc cgatttgtca gcaatgaacc agccagcagg gagggccgag
     4501 cgaagaagtg gtcctgctac tttgtccgcc tccatccagt ctatgagctg ctgtcgtgat
     4561 gctagagtaa gaagttcgcc agtgagtagt ttccgaagag ttgtggccat tgctactggc
     4621 atcgtggtat cacgctcgtc gttcggtatg gcttcgttca actctggttc ccagcggtca
     4681 agccgggtca catgatcacc catattatga agaaatgcag tcagctcctt agggcctccg
     4741 atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca tggtaatggc agcactacac
     4801 aattctctta ccgtcatgcc atccgtaaga tgcttttccg tgaccggcga gtactcaacc
     4861 aagtcgtttt gtgagtagtg tatacggcga ccaagctgct cttgcccggc gtctatacgg
     4921 gacaacaccg cgccacatag cagtactttg aaagtgctca tcatcgggaa tcgttcttcg
     4981 gggcggaaag actcaaggat cttgccgcta ttgagatcca gttcgatata gcccactctt
     5041 gcacccagtt gatcttcagc atcttttact ttcaccagcg tttcggggtg tgcaaaaaca
     5101 ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac gaaaatgttg gatgctcata
     5161 ctcgtccttt ttcaatatta ttgaagcatt tatcagggtt actagtacgt ctctcaagga
     5221 taagtaagta atattaaggt acgggaggta ttggacaggc cgcaataaaa tatctttatt
     5281 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca
     5341 tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg
     5401 tgccagaaca tttctct
//
