LOCUS       sequence-459159-        8931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin 
            Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Qian Y, Celiker OT, Wang Z, Guner-Ataman B, Boyden ES
  TITLE     Temporally multiplexed imaging of dynamic signaling networks in 
            living cells.
  JOURNAL   Cell. 2023 Dec 7;186(25):5656-5672.e21. doi: 
            10.1016/j.cell.2023.11.010. Epub 2023 Nov 28.
  PUBMED    38029746
REFERENCE   2  (bases 1 to 8931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11.010"; journalName: "Cell"; date: "2023-12-7-
            7"; volume: "186"; issue: "25"; pages: "5656-5672.e21"
COMMENT     Sequence Label: pLentiCMV Puro DEST ERKKTRrsFastLime
FEATURES             Location/Qualifiers
     source          1..8931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2220..2446
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2447..2627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2674..2799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3292..3525
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3710..3754
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4021..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4160..4463
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4464..4667
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4618..4638
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4664..4688
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4772..4796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5054..5731
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDGNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     protein_bind    complement(5755..5779)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5810..6398
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5863..5883)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6281..6292)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6412..6911
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6447..6470)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6844..6863
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6932..7531
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6932..6951)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7428..7448
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7669..7902
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7974..8095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8011..8030)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8065..8084
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8135..8270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8290..8309)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8291..8309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8319..8336)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8319..8335)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8328..8350)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8477..8905
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8564..8583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8774..8795
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2641 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     2701 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     2761 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     2821 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     2881 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     2941 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     3001 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     3061 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     3121 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     3181 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     3241 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     3301 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     3361 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     3421 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     3481 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     3541 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3601 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3661 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3721 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     3781 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3841 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3901 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3961 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac
     4021 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4081 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc
     4141 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     4201 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4261 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4321 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     4381 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4441 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     4501 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4561 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4621 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     4681 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct
     4741 agtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctccac
     4801 catgaagggc cgaaagcctc gggacctgga acttccactt agcccaagcc tgctgggtgg
     4861 ccagggacct gaacggactc caggatcagg aacaagctct ggtcttcagg caccggggcc
     4921 agcgctttcg cccagcaagc gtagcggtct cgaggaccca gcgacgccat cgaagaagcc
     4981 acgtacccca tcggtgtcgt caaggctgga gcgcctgacc ctccagtcca gctttcaatt
     5041 cccatccact agtatggtga gtgtgattaa accagacatg aagatcaagc tgcgtatgga
     5101 aggcgctgta aatggacacc cgttcgcgat tgaaggagtt ggccttggga agcctttcga
     5161 gggaaaacag agtatggacc ttaaagtcaa agaaggcgga cctctgcctt tcgcctatga
     5221 catcttgaca actgtgttct gttacggcaa cagggtattc gccaaatacc cagaaaatat
     5281 agtagactat ttcaagcagt cgtttcctga gggctactct tgggaacgaa gcatgaatta
     5341 cgaagacggg ggcatttgta acgcgacaaa cgacataacc ctggatggtg actgttatat
     5401 ctatgaaatt cgatttgatg gtgtgaactt tcctgccaat ggtccagtta tgcagaagag
     5461 gactgtgaaa tgggagccat ccactgagaa attgtatgtg cgtgatggag tgctgaaggg
     5521 tgatggtaac atggctctgt cgcttgaagg aggtggccat taccgatgtg acttcaaaac
     5581 tacttataaa gctaagaagg ttgtccagtt gccagactat cactttgtgg accaccacat
     5641 tgagattaaa agccacgaca aagattacag taatgttaat ctgcatgagc acgccgaagc
     5701 gcattctgag ctgccgaggc aggccaagta ggcggccgca ctcgagatat ctagacccag
     5761 ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgaca atcaacctct
     5821 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     5881 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     5941 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6001 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6061 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6121 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6181 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6241 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6301 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6361 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac cgggtagggg
     6421 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     6481 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     6541 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     6601 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     6661 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     6721 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     6781 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     6841 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     6901 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     6961 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     7021 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     7081 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     7141 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     7201 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     7261 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     7321 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     7381 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     7441 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     7501 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     7561 cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga ccaatgactt
     7621 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag
     7681 ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7741 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7801 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7861 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7921 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     7981 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8041 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8101 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8161 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8221 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     8281 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     8341 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8401 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8461 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8521 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8581 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8641 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     8701 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8761 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     8821 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     8881 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat t
//
