LOCUS       sequence-459162-        9419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR 
            Clover, and P38 KTR Dronpa in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Qian Y, Celiker OT, Wang Z, Guner-Ataman B, Boyden ES
  TITLE     Temporally multiplexed imaging of dynamic signaling networks in 
            living cells.
  JOURNAL   Cell. 2023 Dec 7;186(25):5656-5672.e21. doi: 
            10.1016/j.cell.2023.11.010. Epub 2023 Nov 28.
  PUBMED    38029746
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11.010"; journalName: "Cell"; date: "2023-12-7-
            7"; volume: "186"; issue: "25"; pages: "5656-5672.e21"
COMMENT     Sequence Label: 
            pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P
            38KTRDronpa
FEATURES             Location/Qualifiers
     source          1..9419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   35..164
                     /label=AAV2 ITR
     promoter        487..763
                     /label=chicken beta-actin promoter
     primer_bind     1042..1064
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1126..1145
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1373..2089
                     /codon_start=1
                     /product="reversibly switchable enhanced green fluorescent 
                     protein (Grotjohann et al., 2011)"
                     /label=rsEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTM
                     KFICTTGKLPVPWPTLVTTLTYGVLCFARYPDHMKQHDFFKSAMPEGYVQERTISFEDD
                     GYYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLNSHNAYITADKQKNGIR
                     SNFKIRLNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQNKLSKDPNEKRDHMVLL
                     EFVTASGITLGMDELYK"
     CDS             2096..2434
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVS"
     CDS             2444..2500
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2768..3445
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDGNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     misc_feature    3466..4038
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3633..3650)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3860..3879
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4956..5012
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5217..5894
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     misc_feature    5919..6507
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5972..5992)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6390..6401)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6550..6671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6561..6580)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6615..6634
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7177..7765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7257..7276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7936..8727,8728..8796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8559..8578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8797..8901)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8927..9382)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9064..9085)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9276..9295
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc
       61 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
      121 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
      181 cccgccatgc tacttatcta cgtagccatg ctctaggaag atcgtaggtt aattaaccat
      241 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      301 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      361 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      421 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      481 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      541 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      601 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
      661 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      721 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
      781 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
      841 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
      901 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
      961 ggagggccct ttgtgcgggg ggagcggctc ggggctgtcc gcggggggac ggctgccttc
     1021 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     1081 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     1141 ttgtgctgtc tcatcatttt ggcaaagaat tggatccgcc accatgagta accctaagat
     1201 cctaaaacag agcatgacct tgaacctggc cgacccggtg ggcagtctga agccgcacct
     1261 ccgcgccaag cgctcggccg gtctcaacga agacgagtcg ccatctaaga agctggcgtc
     1321 gccggaggtg tcgtcaaggc tggagcgcct gaccctccag tccagcacta gtatggtgag
     1381 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1441 aaacggccac aagttcagcg tgcgcggcga gggcgagggc gatgccacca acggcaagct
     1501 gaccatgaag ttcatctgca ccaccggcaa gctgccagtg ccctggccca ccctcgtgac
     1561 caccctgacc tacggcgtgc tgtgcttcgc ccgctacccc gaccacatga agcagcacga
     1621 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatca gcttcgagga
     1681 cgacggctat tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1741 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1801 gtacaacctc aacagccaca acgcctatat cacggccgac aagcagaaga acggcatcag
     1861 gtctaacttc aagatccgcc tcaacgtcga ggacggcagc gtgcagctcg ccgaccacta
     1921 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1981 cacccagaat aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2041 gttcgtgacc gcctccggga tcactctcgg catggacgag ctgtacaagc tgcaagttca
     2101 actggtggag tcaggtggtg cacttgttca gccgggcggt agtttgcgac tttcatgcgc
     2161 ggcgtcaggt ttccctgtaa accgatacag tatgaggtgg tatagacaag cgcccggcaa
     2221 agaacgagaa tgggttgcag gaatgagtag tgcaggagat cgaagttcct acgaagacag
     2281 cgttaaaggg cgcttcacta tcagtcgaga tgatgcacgg aacaccgtgt atctgcaaat
     2341 gaacagcctt aaacctgaag atacggccgt atattattgt aacgtcaacg tcggatttga
     2401 atactgggga cagggtacgc aagtcacggt atctggttcc ggagctacta acttcagcct
     2461 gctgaagcag gctggagacg tggaggagaa ccctggacct agatctggtg gctccatgaa
     2521 gggccgaaag cctcgggacc tggaacttcc acttagccca agcctgctgg gtggccaggg
     2581 acctgaacgg actccaggat caggaacaag ctctggtctt caggcaccgg ggccagcgct
     2641 ttcgcccagc aagcgtagcg gtctcgagga cccagcgacg ccatcgaaga agccacgtac
     2701 cccatcggtg tcgtcaaggc tggagcgcct gaccctccag tccagctttc aattcccatc
     2761 cactagtatg gtgagtgtga ttaaaccaga catgaagatc aagctgcgta tggaaggcgc
     2821 tgtaaatgga cacccgttcg cgattgaagg agttggcctt gggaagcctt tcgagggaaa
     2881 acagagtatg gaccttaaag tcaaagaagg cggacctctg cctttcgcct atgacatctt
     2941 gacaactgtg ttctgttacg gcaacagggt attcgccaaa tacccagaaa atatagtaga
     3001 ctatttcaag cagtcgtttc ctgagggcta ctcttgggaa cgaagcatga attacgaaga
     3061 cgggggcatt tgtaacgcga caaacgacat aaccctggat ggtgactgtt atatctatga
     3121 aattcgattt gatggtgtga actttcctgc caatggtcca gttatgcaga agaggactgt
     3181 gaaatgggag ccatccactg agaaattgta tgtgcgtgat ggagtgctga agggtgatgg
     3241 taacatggct ctgtcgcttg aaggaggtgg ccattaccga tgtgacttca aaactactta
     3301 taaagctaag aaggttgtcc agttgccaga ctatcacttt gtggaccacc acattgagat
     3361 taaaagccac gacaaagatt acagtaatgt taatctgcat gagcacgccg aagcgcattc
     3421 tgagctgccg aggcaggcca agtaaagaag gatcttagct taaggcccct ctccctcccc
     3481 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     3541 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     3601 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     3661 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     3721 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     3781 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     3841 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     3901 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     3961 tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     4021 tgaaaaacac gatgataatg gccacagaat tcatggagat gccagaggaa cccgccaaca
     4081 gtgggcattc actacccccg gtttatattt acagtcccga gtatgtcagc atctgcgact
     4141 cccttgtgaa ggtccccaaa cgggccagta tggtcaacga agacgaggcg ccatctagaa
     4201 ggaaggcgtc gggtcaggtg tcgtcaaggc tggagcgcct gaccctccag tccagcacta
     4261 gtatggtgag caagggcgag gagctgttca ccggggttgt gcccatcctg gtcgagctgg
     4321 acggcgacgt aaacggccac aagttcagcg tccgcggcga gggcgagggc gatgccacca
     4381 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4441 ccctcgtgac caccttcggc tacggcgtgg cctgcttcag ccgctacccc gaccacatga
     4501 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4561 ctttcaagga cgacggtacc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4621 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4681 acaagctgga gtacaacttc aacagccaca acgtctatat cacggccgac aagcagaaga
     4741 acggcatcaa ggctaacttc aagatccgcc acaacgttga ggacggcagc gtgcagctcg
     4801 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4861 actacctgag ccatcagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4921 tcctgctgga gttcgtgacc gccgccggtt ccggagctac taacttcagc ctgctgaagc
     4981 aggctggaga cgtggaggag aaccctggac ctagatctgg tggctccatg cgtaagccag
     5041 atctccgcgt tcttatccca cctggcagca agaacacgat gccatcagtg aatcaaagga
     5101 taaataactc caagcgctcg gccggtctca acgaagacga gtcgccatct aagaagctgg
     5161 cgtcgccgga ggtgtcgtca aggctggagc gcctgaccct ccagtccagc actagtatgg
     5221 tgagtgtgat taaaccagac atgaagatca agctgcgtat ggaaggcgct gtaaatggac
     5281 acccgttcgc gattgaagga gttggccttg ggaagccttt cgagggaaaa cagagtatgg
     5341 accttaaagt caaagaaggc ggacctctgc ctttcgccta tgacatcttg acaactgtgt
     5401 tctgttacgg caacagggta ttcgccaaat acccagaaaa tatagtagac tatttcaagc
     5461 agtcgtttcc tgagggctac tcttgggaac gaagcatgaa ttacgaagac gggggcattt
     5521 gtaacgcgac aaacgacata accctggatg gtgactgtta tatctatgaa attcgatttg
     5581 atggtgtgaa ctttcctgcc aatggtccag ttatgcagaa gaggactgtg aaatgggagc
     5641 catccactga gaaattgtat gtgcgtgatg gagtgctgaa gggtgatgtt aacatggctc
     5701 tgtcgcttga aggaggtggc cattaccgat gtgacttcaa aactacttat aaagctaaga
     5761 aggttgtcca gttgccagac tatcactttg tggaccacca cattgagatt aaaagccacg
     5821 acaaagatta cagtaatgtt aatctgcatg agcacgccga agcgcattct gagctgccga
     5881 ggcaggccaa gtgagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     5941 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6001 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6061 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6121 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6181 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6241 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6301 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     6361 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6421 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6481 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acccgggcgg ccgcttcgag
     6541 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     6601 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     6661 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     6721 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatcttc
     6781 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     6841 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgcccgg
     6901 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcattaat gaatcggcca
     6961 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8221 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8281 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8341 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8401 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8461 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8521 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8581 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8641 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8701 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8761 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8821 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     8941 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     9001 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     9061 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     9121 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     9181 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     9241 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     9301 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     9361 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaagg
//
