LOCUS       sequence-459209-        8352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of poly H35R SUMO1 protein under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Kim J, Qin S, Zhou HX, Rosen MK
  TITLE     Surface Charge Can Modulate Phase Separation of Multidomain 
            Proteins.
  JOURNAL   J Am Chem Soc. 2024 Feb 7;146(5):3383-3395. doi: 
            10.1021/jacs.3c12789. Epub 2024 Jan 23.
  PUBMED    38262618
REFERENCE   2  (bases 1 to 8352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c12789"; journalName: "J Am Chem Soc"; date: 
            "2024-02-7- 7"; volume: "146"; issue: "5"; pages: "3383-3395"
COMMENT     Sequence Label: poly H35R SUMO1
FEATURES             Location/Qualifiers
     source          1..8352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(447..452,453..468,469..475)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    483..499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     488..510
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             569..1669
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1526..1535
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1643..1666
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1718..1729
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1742..1762
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3410..3430
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3431..3448
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3461..3478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3461..3477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3470..3492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3656..3673)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3826..3912
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4004..4031
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4050..4141
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4142..4210,4211..5002)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4360..4379)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5044..5557)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5239..5260)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5451..5470
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5668..6256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6157..6176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6410..6427
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6533..6552)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6693..6715
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6709..6900)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        7396..7473
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             join(7474..8352,1..204)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7493..7512)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
       61 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
      121 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      181 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta
      241 ggcacaattc tcatgtttga cagcttatca tcgactgcac ggtgcaccaa tgcttctggc
      301 gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc
      361 gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt
      421 tctggcaaat attctgaaat gagctgttga caattaatca tcggctcgta taatgtgtgg
      481 aattgtgagc ggataacaat ttcacacagg aaacagccag tccgtttagg tgttttcacg
      541 agcacttcac caacaaggac catagattat gaaaactgaa gaaggtaaac tggtaatctg
      601 gattaacggc gataaaggct ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga
      661 taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat tcccacaggt
      721 tgcggcaact ggcgatggcc ctgacattat cttctgggca cacgaccgct ttggtggcta
      781 cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg acaagctgta
      841 tccgtttacc tgggatgccg tacgttacaa cggcaagctg attgcttacc cgatcgctgt
      901 tgaagcgtta tcgctgattt ataacaaaga tctgctgccg aacccgccaa aaacctggga
      961 agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa
     1021 cctgcaagaa ccgtacttca cctggccgct gattgctgct gacgggggtt atgcgttcaa
     1081 gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg gataacgctg gcgcgaaagc
     1141 gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag acaccgatta
     1201 ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg
     1261 ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt
     1321 caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta acgccgccag
     1381 tccgaacaaa gagctggcaa aagagttcct cgaaaactat ctgctgactg atgaaggtct
     1441 ggaagcggtt aataaagaca aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga
     1501 gttggcgaaa gatccacgta ttgccgccac catggaaaac gcccagaaag gtgaaatcat
     1561 gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc
     1621 cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagacta attcgagctc
     1681 gaacaacaac aacaataaca ataacaacaa cctcgggatc gagggaagga tttcagaatt
     1741 tgaaaacctg tattttcagg gccatatggg aggcggctca ggtggttcta tgtctgatca
     1801 agaggcgaag ccatccacgg aggacttggg agataaaaag gagggggaat atattaaact
     1861 taaggtcatc ggtcaagatt catcagaaat ccggtttaaa gtgaaaatga cgacgcactt
     1921 gaaaaaatta aaagaatcct actgccagcg gcaaggcgta ccgatgaata gcttgcggtt
     1981 tttgttcgaa ggacagcgta ttgccgacaa tcatactcca aaagaactcg gaatggaaga
     2041 ggaagatgtc attgaggtct accaagaaca aacagttgta gggggatctg gtggctctgg
     2101 cggaagtggc gggtcgatgt cggaccaaga ggcgaaacca agtaccgagg acctgggaga
     2161 caagaaggaa ggtgagtata tcaagctcaa agttattggg caggactcat ctgaaattcg
     2221 gtttaaggtt aaaatgacca ctcatcttaa aaaactgaag gagtcctatt gccagcgtca
     2281 gggggtacca atgaactcac tgcgtttcct ctttgaggga cagcgcatcg cggataacca
     2341 taccccaaag gaacttggaa tggaggagga agatgtgatc gaagtttacc aagagcaaac
     2401 cgtggttggc ggttctgggg gctctggggg gtcggggggc agcatgtccg atcaagaggc
     2461 taaaccatct actgaagatc tcggggataa aaaggaggga gaatatatca agttaaaagt
     2521 aatcggacaa gactcaagtg aaatccgctt caaagtaaag atgaccaccc atctcaaaaa
     2581 gcttaaagag tcttattgtc agcgccaagg ggtgccaatg aatagcctgc ggtttctttt
     2641 cgaaggtcag cggattgcag acaaccatac acctaaggag ttaggtatgg aggaggagga
     2701 tgtcatcgaa gtctatcaag aacagacagt cgttggagga tctggcggct ccggtgggtc
     2761 gggcggtagc atgtcagatc aagaagccaa accgagcaca gaagacttag gggacaaaaa
     2821 agaaggggaa tacattaagt taaaggtgat tggccaagac agctcggaaa tcagattcaa
     2881 ggtaaaaatg actacacacc ttaagaaatt aaaggaatct tactgtcaac ggcaaggtgt
     2941 ccctatgaac tctttgcgtt ttctgttcga agggcaacgt attgccgata accacacccc
     3001 gaaggagctc ggcatggaag aagaagacgt gattgaagtg tatcaagaac aaaccgtcgt
     3061 cggtgggagc gggggtagcg gtggaagtgg aggttcaatg agtgatcagg aagcgaagcc
     3121 gtcgacggaa gacctcggtg ataaaaagga gggcgagtac atcaaactta aagtaattgg
     3181 tcaggatagc tctgagattc gttttaaagt caaaatgaca acccacctga agaaacttaa
     3241 ggaaagctat tgccaacgcc agggagtccc gatgaattcg cttcgctttt tatttgaagg
     3301 tcagcgtatc gctgataatc acactccgaa agagttgggg atggaggagg aggatgttat
     3361 tgaggtgtac caagaacaga ccgtagtagg cggctcctgt ggtggatccg aaaacctgta
     3421 ttttcagggc catcatcatc atcatcactg aaagcttggc actggccgtc gttttacaac
     3481 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     3541 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3601 gcctgaatgg cgaatggcag cttggctgtt ttggcggatg agataagatt ttcagcctga
     3661 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
     3721 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
     3781 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     3841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     3901 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     3961 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
     4021 gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat
     4081 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4141 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4201 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4261 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4321 cgaagaacgt ttcccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4381 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4441 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4501 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4561 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4621 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4681 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4741 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4801 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4861 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4921 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4981 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5041 tttaccccgg ttgataatca gaaaagcccc aaaaacagga agattgtata agcaaatatt
     5101 taaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     5161 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagcccgag
     5221 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     5281 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     5341 aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     5401 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     5461 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     5521 acacccgccg cgcttaatgc gccgctacag ggcgcgtaaa aggatctagg tgaagatcct
     5581 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5641 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5701 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5761 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5821 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5881 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5941 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6001 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6061 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6121 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6181 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6241 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6301 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6361 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6421 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     6481 ttcacaccgc atgtggtgca ctctcagtac accgcatgtg gtgcactctc agtacaatct
     6541 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     6601 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     6661 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     6721 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag
     6781 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     6841 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     6901 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga
     6961 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga
     7021 gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg
     7081 ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat
     7141 gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac
     7201 acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc
     7261 gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     7321 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac
     7381 gctgcccgaa attccgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag
     7441 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc
     7501 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac
     7561 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc
     7621 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc
     7681 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa
     7741 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg
     7801 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat
     7861 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat
     7921 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg
     7981 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta
     8041 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa
     8101 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc
     8161 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
     8221 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg
     8281 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc
     8341 aaacaggatt tt
//
