LOCUS       sequence-459270-        8887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA targeting Cdkn1a, mCherry, puromycin resistance, Mammalian 
            expression, Mouse targeting, lentiviral, CRISPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Boutelle AM, Mabene AR, Yao D, Xu H, Wang M, Tang YJ, Lopez SS, 
            Sinha S, Demeter J, Cheng R, Benard BA, McCrea EM, Valente LJ, 
            Drainas AP, Fischer M, Majeti R, Petrov DA, Jackson PK, Yang F, 
            Winslow MM, Bassik MC, Attardi LD
  TITLE     Integrative multiomic approaches reveal ZMAT3 and p21 as conserved 
            hubs in the p53 tumor suppression network.
  JOURNAL   Cell Death Differ. 2025 Apr 22. doi: 10.1038/s41418-025-01513-8.
  PUBMED    40263541
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2025 Apr 22. doi: 10.1038/s41418-025-01513-8."
COMMENT     Sequence Label: pMCB320 sgCdkn1a.2
FEATURES             Location/Qualifiers
     source          1..8887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             750..930
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    977..1102
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1599..1832
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2017..2061
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2359..2476
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2729..2762)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2766..2786
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3029..3240
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3241..4179
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4136..4156
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4200..4796
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4200..4219)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4696..4716
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4806..4859
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4866..4875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4872..5582
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5017..5035)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5285..5304
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(5602..5635)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5691..6279
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5744..5764)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6162..6173)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6282..6298)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6283..6299)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6808..6988
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7050..7638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7130..7149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7809..8600,8601..8669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8432..8451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8670..8774)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8849..8868)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
       61 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
      121 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      781 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      841 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      901 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      961 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     1021 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     1081 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     1141 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1201 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1261 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1321 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1381 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1441 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1501 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1561 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1621 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1681 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1741 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1801 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1861 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1921 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1981 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     2041 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     2101 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     2161 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2221 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2281 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     2341 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2401 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2461 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc
     2521 gggcccgctc tagagatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacgga
     2581 cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag
     2641 taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct
     2701 acattttaca tgataggctt ggatttctat aacttcgtat agcatacatt atacgaagtt
     2761 ataaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat
     2821 aagtatccct tggagaacca ccttgttggt gattgcgatg cgctcagttt aagagctaag
     2881 ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca
     2941 ccgagtcggt gctttttttc tcgagtacta ggatccatta ggcggccgcg tggataaccg
     3001 tattaccgcc atgcatgtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     3061 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     3121 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     3181 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     3241 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     3301 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     3361 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     3421 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     3481 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     3541 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     3601 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     3661 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     3721 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     3781 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     3841 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     3901 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     3961 cacctcgatt agttctcgcg cttttggagt acgtcgtctt taggttgggg ggaggggttt
     4021 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     4081 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     4141 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagc tagcccacca
     4201 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     4261 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     4321 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     4381 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     4441 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     4501 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     4561 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     4621 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     4681 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     4741 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccggat
     4801 cgggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat cctggcccac
     4861 cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     4921 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     4981 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     5041 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     5101 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     5161 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     5221 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     5281 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     5341 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     5401 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     5461 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     5521 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     5581 aagaattcgt cgagggacct aataacttcg tatagcatac attatacgaa gttatacatg
     5641 tttaagggtt ccggttccac taggtacaat tcgatatcaa gcttatcgat aatcaacctc
     5701 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5761 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5821 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5881 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5941 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6001 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6061 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6121 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6181 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6241 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgat
     6301 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6361 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     6421 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6481 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     6541 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     6601 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     6661 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     6721 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     6781 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6841 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6901 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6961 ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa aaggccagca aaaggccagg
     7021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7081 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7501 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7561 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7921 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8101 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8401 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8761 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8821 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8881 agtatct
//
