LOCUS       sequence-459298-       10306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral helper virus for G-deleted rabies tracing, with U6-driven
            transcript amenable for nuclear capture in the presence of RVdG 
            expressing MS2-KASH..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Shin D, Urbanek ME, Larson HH, Moussa AJ, Lee KY, Baker DL, 
            Standen-Bloom E, Ramachandran S, Bogdanoff D, Cadwell CR, Nowakowski
            TJ
  TITLE     High-Complexity Barcoded Rabies Virus for Scalable Circuit Mapping 
            Using Single-Cell and Single-Nucleus Sequencing.
  JOURNAL   bioRxiv [Preprint]. 2024 Dec 11:2024.10.01.616167. doi: 
            10.1101/2024.10.01.616167.
  PUBMED    39713304
REFERENCE   2  (bases 1 to 10306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Dec 11:2024.10.01.616167. doi: 
            10.1101/2024.10.01.616167."
COMMENT     Sequence Label: 
            pSico-U6-tornado:6xMBS:8G8ApA-syn-GFP-TVA-oG-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..10306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..704
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             751..931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(949..1147)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1149..1528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1700..1719
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1794..1898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1899..1967,1968..2759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2117..2136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2930..3518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3419..3438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3580..3760
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        4147..4395
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4147..4167
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4318..4337
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     4843..4862
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4843..4861
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5107..5554
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      5569..5578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5575..5577,5578..5580,5581..6291)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5575..5577,5578..5580,5581..6291)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5620..5641)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5881..5900)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6228..6249
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6301..6354
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6841..6900
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     misc_feature    8526..9114
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8579..8599)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8997..9008)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9229..9453
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    9511..9628
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9926..9970)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(10155..10306,1..82)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa
       61 gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct
      121 ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa
      181 ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccggcc
      241 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
      301 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
      361 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
      421 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
      481 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
      541 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
      601 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
      661 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
      721 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
      781 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
      841 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
      901 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag
      961 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     1021 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     1081 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     1141 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     1201 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     1261 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     1321 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     1381 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     1441 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     1501 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     1561 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     1621 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     1681 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
     1741 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2761 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2821 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3061 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3541 tacggttcct ggccttttgc tggccttttg ctcacatgct gctagagatt ttccacactg
     3601 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     3661 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     3721 agagctccca ggctcagatc tggtctaacc agagagaccc agtacagtcc ggatgcagct
     3781 ctcgggccat gtgatgaaat gctaggcggc tgtcaaacct ccactctaat acttctctct
     3841 ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gcgggtgttc tctccttcat
     3901 tggcttcttc taccttctct tgctcaactg gtactagctt gtagcaccat ccaaaggtca
     3961 gtggatatct gatccctggc cctggtgtgt agttctgcca atcagggaag tagccttgtg
     4021 tgtggtagat ccacagatca aggatatctt gtcttcgttg ggagtgaatt agcccttcca
     4081 gtcccccctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa gtcattggtc
     4141 ttaaaggagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     4201 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     4261 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     4321 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     4381 aaaggacgaa acaccgtcac cggccgcact cgccggtccc aagcccggat aaaatgggag
     4441 ggggcgggaa accgcctaac catgccgagt gcggccgcaa cctacaaacg aacctacaaa
     4501 cgggtggagg atcaccccac ccgacacttc acaatcaagg ggtacaatac acaagggtgg
     4561 aggaacaccc caccctccag acacattaca cagaaatcca atcaaacaga agcaccatca
     4621 gggcttctgc taccaaattt atctcaaaaa actacaacaa ggaatcacca tcagggattc
     4681 cctgtgcaat atacgtcaaa cgagggccac gacgggagga cgatcacgcc tcccgaatat
     4741 cggcatgtct ggctttcgaa ttcagtgcgt ggagcatcag cccacgcagc caatcagagt
     4801 cggcgagaag gtcactgccc gtatgtgcgg acacagggat tttaatacga ctcactatag
     4861 ggataatttc gaattctgca gtcgacggta ccgttaaccc ttggcacccg agaattccag
     4921 ttaaccgcta gcttttcagt cgagaaaaaa aagaaaaaaa gaaaaaaaga aaaagtggcc
     4981 gcggtcggcg tggactgtag aacactgcca atgccggtcc caagcccgga taaaagtgga
     5041 gggtacagtc cacgcttttt tctaattagg cggccgcgta ttaccgccat gcatgtgccc
     5101 gtcagtagtg caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac
     5161 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct
     5221 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc
     5281 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga
     5341 aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg
     5401 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca
     5461 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg
     5521 cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga aaccggtcgc caccatggtg
     5581 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     5641 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     5701 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     5761 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     5821 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     5881 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     5941 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     6001 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     6061 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     6121 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     6181 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     6241 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggggtccggg
     6301 gaagggcgcg gttccttgct tacgtgtggt gacgtggagg agaatcctgg gcctcgcacg
     6361 atggcaaggc ttcttccagc ccttcttctg ctcctgctgc caggcaatgt aacggggaac
     6421 ggctcaggta atggatcatt gtcccggtgt ccaccaggtc aattcaggtg tagtgagccc
     6481 ccgggcgcac atggggaatg ttacccacag gattggcttt gcgacgggca tccagactgc
     6541 gacgatggaa gagatgaatg gggctgtggt acaagtgcaa ttcctgcggt gcctacggat
     6601 aacggaactg aggcacccac ggctccagct ccaggtcggg cgcttcccgc acggaatcac
     6661 ggacggatgt ggatgctgat aacagcggtt ttgctctgtt gtctcgtagc cgtcgggggg
     6721 atagccgcat ggggcaagtc caaagcaaag agccgctctg atattttttc attggagtca
     6781 gcttctaagg aacttctcgt cccggataag agtcaggcgg acctgttcag tggttcagga
     6841 cagtgcacga attatgcctt gctcaaattg gccggagacg tcgagtcaaa cccaggtcct
     6901 gcaggcacca tggtgcccca agcgctgttg ttcgttcctc tgttggtgtt cccactgtgc
     6961 tttggtaaat tccctattta caccataccg gataagctcg gtccgtggtc tcctatcgac
     7021 atccaccatt tgtcttgccc taacaacctg gtagtagaag atgagggatg caccaacttg
     7081 agtgggttct cctatatgga gctcaaggta gggtatattt cagccatcaa aatgaatggt
     7141 tttacgtgta caggtgttgt aactgaagct gagacataca ctaactttgt cgggtatgta
     7201 accaccacgt ttaaaagaaa acattttaga ccaacgcctg acgcgtgccg agcagcttac
     7261 aattggaaaa tggctggcga tccgaggtat gaagagtcac tgcataatcc ctacccagat
     7321 taccattggt tgcgaactgt gaaaacgaca aaagaatccc tcgtaatcat tagtccctca
     7381 gtcgcagatc tggaccctta cgaccggagc ctgcacagtc ctgtgttccc gggcggcaat
     7441 tgtagcggcg tagcggtgag ttccacctat tgcagcacca accatgatta cacgatctgg
     7501 atgccggaga atcccaggtt gggtatgagt tgtgacatat tcactaactc tcgcggcaag
     7561 cgggcgtcca agggtagtga gacgtgtgga tttgtagatg aaaggggcct ttacaaatca
     7621 ttgaaaggtg cctgcaaact caaactctgc ggcgtccttg gtcttcggtt gatggatgga
     7681 acctgggtgg cgatgcaaac ttccaacgag acaaagtggt gtccaccagg ccagctggtg
     7741 aatctccacg acttccggag tgatgaaata gaacatttgg tcgttgaaga acttgtgaag
     7801 aagagagaag agtgcctgga cgctctcgaa tctattatga ctactaaatc tgtttctttc
     7861 cgccggttgt cccatcttag gaaacttgta ccaggctttg ggaaagcata tacaattttc
     7921 aacaaaactc tcatggaggc tgacgcgcat tacaagtctg ttcggacttg gaacgagatc
     7981 ataccatcca aggggtgctt gcgggttggt ggtagatgcc acccccacgt aaacggcgta
     8041 ttcttcaatg gtattatcct tggccccgac ggcaacgtgc tgattccaga gatgcaaagc
     8101 tcccttttgc aacaacacat ggagttgctc gtttcttccg tcataccact tatgcatcct
     8161 ctcgcagacc ccagtacggt attcaaaaat ggtgatgagg ccgaagactt tgtagaggtc
     8221 cacttgccag atgtacatga acgaatcagt ggggtagacc tcgggcttcc aaactggggc
     8281 aagtacgttc ttctcagtgc cggagcgttg accgccctca tgctgataat attcctcatg
     8341 acgtgctggc ggagagtgaa cagatccgaa ccaacccaac ataacctgcg agggacaggt
     8401 cgggaagtgt ctgtaactcc acaatccggg aaaatcatat cttcctggga gagccataag
     8461 tctggtggtg agacccgact gtaatttgta gcggccgcta caaagcttat gccgacgtac
     8521 gatccaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     8581 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     8641 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     8701 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     8761 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     8821 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     8881 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     8941 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     9001 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     9061 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcctg
     9121 gacctcccta gcaaactggg gcacaagcga attcccacct aggtagggat ccaagggtac
     9181 cttcggcgcg ccgtatgatc attggctagc tcgacatgtc tactcgagct gtgccttcta
     9241 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     9301 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     9361 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     9421 gcaggcatgc tggggatgcg gtgggctcta tggctagagc gggcccggta ctaaccaaac
     9481 tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
     9541 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
     9601 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt gtctgcagaa
     9661 ttggcgcacg cagtgccgat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     9721 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     9781 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     9841 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     9901 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     9961 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
    10021 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
    10081 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
    10141 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
    10201 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
    10261 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattg
//
