LOCUS       sequence-459309-       10604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minigene of human MAPT containing exon 9, 10, 11 and relevant 
            intronic sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Recinos Y, Bao S, Wang X, Phillips BL, Yeh YT, Weyn-Vanhentenryck 
            SM, Swanson MS, Zhang C
  TITLE     Lineage-specific splicing regulation of MAPT gene in the primate 
            brain.
  JOURNAL   Cell Genom. 2024 Jun 12;4(6):100563. doi: 
            10.1016/j.xgen.2024.100563. Epub 2024 May 20.
  PUBMED    38772368
REFERENCE   2  (bases 1 to 10604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2024-06-12"; volume: "4(6):100563. doi"; pages: " 
            10.1016/j.xgen.2024.100563. Epub 2024 May 20"
COMMENT     Sequence Label: pcDNA5/FRT-hMAPT
FEATURES             Location/Qualifiers
     source          1..10604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3599..3616)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3605..3829
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3962..3981)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4113..4160
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4168..5190
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4168..5190
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5320..5441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5357..5376)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5411..5430
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5490..5506)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5490..5506)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5503..5525)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5514..5530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5538..5544,5545..5562,5563..5568))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5583..5604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5721..5738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5892..6480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5972..5991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6651..7442,7443..7511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7274..7293
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7512..7616)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7691..7710)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7882..8261
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8262..8465
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8416..8436
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8510..8529
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8510..8528
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagccccata gcccattgga agttgggctg aaggtggtgg cacctgagac tgggctgccg
       61 cctcctcccc cgacacctgg gcaggttgac gttgagtggc tccactgtgg acaggtgacc
      121 cgtttgttct gatgagcgga caccaaggtc ttactgtcct gctcagctgc tgctcctaca
      181 cgttcaaggc aggagccgat tcctaagcct ccagcttatg cttagcctgc gccaccctct
      241 ggcagagact ccagatgcaa agagccaaac caaagtgcga caggtccctc tgcccagcgt
      301 tgaggtgtgg cagagaaatg ctgcttttgg cccttttaga tttggctgcc tcttgccagg
      361 agtggtggct cgtgcctgta attccagcac tttgggagac taaggcggga ggttcgcttg
      421 agcccaggag ttcaagacca gcctgggcaa caatgagacc cctgtgtcta caaaaagaat
      481 taaaattagc caggtgtggt ggcacgcacc tgtagtccca gctacttggg aggctgaggt
      541 gggaggattg cctgagtccg ggaggcggaa gttgcaagga gccatgatcg cgccactgca
      601 cttcaaccta ggcaacagag tgagactttg tctcaaaaaa caatcatata ataattttaa
      661 aataaataga tttggcttcc tctaaatgtc cccggggact ccgtgcatct tctgtggagt
      721 gtctccgtga gattcgggac tcagatcctc aagtgcaact gacccacccg ataagctgag
      781 gcttcatcat cccctggccg gtctatgtcg actgggcacc cgaggctcct ctcccaccag
      841 ctctcttggt cagctgaaag caaactgtta acaccctggg gagctggacg tatgagaccc
      901 ttggggtggg aggcgttgat ttttgagagc aatcacctgg ccctggctgg cagtaccggg
      961 acactgctgt ggctccgggg tgggctgtct ccagaaaatg cctggcctga ggcagccacc
     1021 cgcatccagc ccagagggtt tattcttgca atgtgctgct gcttcctgcc ctgagcacct
     1081 ggatcccggc ttctgccctg aggccccttg agtcccacag gtagcaagcg cttgccctgc
     1141 ggctgctgca tggggctaac taacgcttcc tcaccagtgt ctgctaagtg tctcctctgt
     1201 ctcccacgcc ctgctctcct gtccccccag tttgtctgct gtgaggggac agaagaggtg
     1261 tgtgccgccc ccacccctgc ccgggccctt gttcctggga ttgctgtttt cagctgtttg
     1321 agctttgatc ctggttctct ggcttcctca aagtgagctc ggccagagga ggaaggccat
     1381 gtgctttctg gttgaagtca agtctggtgc cctggtggag gctgtgctgc tgaggcggag
     1441 ctggggagag agtgcacacg ggctgcgtgg ccaacccctc tgggtagctg atgcccaaag
     1501 acgctgcagt gcccaggaca tctgggacct ccctggggcc cgcccgtgtg tcccgcgctg
     1561 tgttcatctg cgggctagcc tgtgacccgc gctgtgctcg tctgcgggct agcctgtgtc
     1621 ccgcgctctg cttgtctgcg gtctagcctg tgacctggca gagagccacc agatgtcccg
     1681 ggctgagcac tgccctctga gcaccttcac aggaagccct tctcctggtg agaagagatg
     1741 ccagcccctg gcatctgggg gcactggatc cctggcctga gccctagcct ctccccagcc
     1801 tgggggcccc ttcccagcag gctggccctg ctccttctct acctgggacc cttctgcctc
     1861 ctggctggac cctggaagct ctgcagggcc tgctgtcccc ctccctgccc tccaggtatc
     1921 ctgaccaccg gccctggctc ccactgccat ccactcctct cctttctggc cgttccctgg
     1981 tccctgtccc agcccccctc cccctctcac gagttacctc acccaggcca gagggaagag
     2041 ggaaggaggc cctggtcata ccagcacgtc ctcccacctc cctcggccct ggtccacccc
     2101 ctcagtgctg gcctcagagc acagctctct ccaagccagg ccgcgcgcca tccatcctcc
     2161 ctgtccccca acgtccttgc cacagatcat gtccgccctg acacacatgg gtctcagcca
     2221 tctctgcccc agttaactcc ccatccataa agagcacatg ccagccgaca ccaaaataat
     2281 tcgggatggt tccagtttag acctaagtgg aaggagaaac caccacctgc cctgcacctt
     2341 gttttttggt gaccttgata aaccatcttc agccatgaag ccagctgtct cccaggaagc
     2401 tccagggcgg tgcttcctcg ggagctgact gataggtggg aggtggctgc ccccttgcac
     2461 cctcaggtga ccccacacaa ggccactgct ggaggccctg gggactccag gaatgtcaat
     2521 cagtgacctg ccccccaggc cccacacagc catggctgca tagaggcctg cctccaaggg
     2581 acctgtctgt ctgccactgt ggagtcccta cagcgtgccc cccacagggg agctggttct
     2641 ttgactgaga tcagctggca gctcagggtc atcattccca gagggagcgg tgccctggag
     2701 gccacaggcc tcctcatgtg tgtctgcgtc cgctcgagct tactgagaca ctaaatctgt
     2761 tggtttctgc tgtgccacct acccaccctg ttggtgttgc tttgttccta ttgctaaaga
     2821 caggaatgtc caggacactg agtgtgcagg tgcctgctgg ttctcacgtc cgagctgctg
     2881 aactccgctg ggtcctgctt actgatggtc tttgctctag tgctttccag ggtccgtgga
     2941 agcttttcct ggaataaagc ccacgcatcg accctcacag cgcctcccct ctttgaggcc
     3001 cagcagatac cccactcctg cctttccagc aagatttttc agatgctgtg catactcatc
     3061 atattgatca cttttttctt catgcctgat tgtgatctgt caatttcatg tcaggaaagg
     3121 gagtgacatt tttacactta agcgtttgct gagcaaatgt ctgggtcttg cacaatgaca
     3181 atgggtccct gtttttccca gaggctcttt tgttctgcag ggattgaaga cactccagtc
     3241 ccacagtccc cagctcccct ggggcagggt tggcagaatt tcgacaacac atttttccac
     3301 cctgactagg atgtgctcct catggcagct gggaaccact gtccaataag ggcctgggct
     3361 tacacagctg cttctcattg agttacaccc ttaataaaat aatcccattt tatccttttt
     3421 gtctctctgt cttcctctct ctctgccttt cctcttctct ctcctcctct ctcatctcca
     3481 ggtgcaaata gtctacaaac cagttgacct gagcaaggtg acctccaagt gtggctcatt
     3541 aggcaacatc catcataaac cagtgagtct agagggcccg tttaaacccg ctgatcagcc
     3601 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3661 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3721 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     3781 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     3841 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     3901 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3961 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4021 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4081 aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct
     4141 ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag
     4201 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     4261 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     4321 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     4381 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     4441 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     4501 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     4561 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     4621 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     4681 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     4741 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     4801 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     4861 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     4921 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     4981 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     5041 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     5101 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     5161 cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga ttccaccgcc
     5221 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     5281 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     5341 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5401 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     5461 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5521 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5581 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5641 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5701 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5761 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5821 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5881 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5941 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6001 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6061 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6121 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6181 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6241 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6301 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6361 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6421 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6481 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6541 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6601 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6661 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6721 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6781 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6841 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6901 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6961 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7021 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7081 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7141 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7201 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7261 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7321 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7381 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7441 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7501 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7561 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7621 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     7681 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     7741 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     7801 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     7861 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     7921 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     7981 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     8041 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     8101 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     8161 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     8221 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     8281 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     8341 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     8401 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     8461 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
     8521 actataggga gacccaagct ggctagcgtt taaacttagt gaacctccaa aatcagggga
     8581 tcgcagcggc tacagcagcc ccggctcccc aggcactccc ggcagccgct cccgcacccc
     8641 gtcccttcca accccaccca cccgggagcc caagaaggtg gcagtggtcc gtactccacc
     8701 caagtcgccg tcttccgcca agagccgcct gcagacagcc cccgtgccca tgccagacct
     8761 gaagaatgtc aagtccaaga tcggctccac tgagaacctg aagcaccagc cgggaggcgg
     8821 gaaggtgaga gtggctggct gcgcgtggag gtgtgggggg ctgcgcctgg aggggtaggg
     8881 ctgtgcctgg aagggtaggg ctgcgcctgg aggtgcgcgg ttgagcgtgg agtcgtggga
     8941 ctgtgcatgg aggtgtgggg ctccccgcac ctgagcaccc ccgcataaca ccccagtccc
     9001 ctctggaccc tcttcaagga agttcagttc tttattgggc tctccactac actgtgagtg
     9061 ccctcctcag gcgagagaac gttctggctc ttctcttgcc ccttcagccc ctgttaatcg
     9121 gacagagatg gcagggctgt gtctccacgg ccggaggctc tcatagtcag ggcacccaca
     9181 gcggttcccc acctgccttc tgggcagaat acactgccac ccataggtca tttctcatca
     9241 aaacgctgat attttcacac ctccctggtg tctgcagaaa gaaccttcca gaaatgcagt
     9301 cgtgggagac ccatccaggc cacccctgct tatggaagag ctgagaaaaa gccccacggg
     9361 agcatttgct cagcttccgt tacgcaccta gtggcattgt gggtgggaga gggctggtgg
     9421 gtggatggaa ggagaaggca cagccccccc ttgcagggac agagccctcg tacagaaggg
     9481 acaccccaca tttgtcttcc ccacaaagcg gcctgtgtcc tgcctacggg gtcagggctt
     9541 ctcaaacctg gctgtgtgtc agaatcacca ggggaacttt tcaaaactag agagactgaa
     9601 gccagactcc tagattctaa ttctaggtca gggctagggg ctgagattgt aaaaatccac
     9661 aggtgattct gatgcccggc aggcttgaga acagccgcag ggagttctct gggaatgtgc
     9721 cggtgggtct agccaggtgt gagtggagat gccggggaac ttcctattac tcactcgtca
     9781 gtgtggccga acacattttt cacttgacct caggctggtg aacgctcccc tctggggttc
     9841 aggcctcacg atgccatcct tttgtgaagt gaggacctgc aatcccagct tcgtaaagcc
     9901 cgctggaaat cactcacact tctgggatgc cttcagagca gccctctatc ccttcagctc
     9961 ccctgggatg tgactcgacc tcccgtcact ccccagactg cctctgccaa gtccgaaagt
    10021 ggaggcatcc ttgcgagcaa gtaggcgggt ccagggtggc gcatgtcact catcgaaagt
    10081 ggaggcgtcc ttgcgagcaa gcaggcgggt ccagggtggc gtgtcactca tccttttttc
    10141 tggctaccaa aggtgcagat aattaataag aagctggatc ttagcaacgt ccagtccaag
    10201 tgtggctcaa aggataatat caaacacgtc ccgggaggcg gcagtgtgag taccttcaca
    10261 cgtcccatgc gccgtgctgt ggcttgaatt attaggaagt ggtgtgagtg cgtacacttg
    10321 cgagacactg catagaataa atccttcttg ggctctcagg atctggctgc gacctctggg
    10381 tgaatgtagc ccggctcccc acattccccc acacggtcca ctgttcccag aagccccttc
    10441 ctcatattct aggagggggt gtcccagcat ttctgggtcc cccagcctgc gcaggctgtg
    10501 tggacagaat agggcagatg acggaccctc tctccggacc ctgcctggga agctgagaat
    10561 acccatcaaa gtctccttcc actcatgccc agccctgtcc ccag
//
