LOCUS       sequence-459317-        3373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified version of the pBLADE_ONLY_C (#168050). It expresses 
            VVD-AraC, but the f1 ori was deleted and the chloramphenicol 
            resistance gene was replaced by gentamicin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Hollo G, Park JH, Boni E, Schaerli Y
  TITLE     A tool for modeling gene regulatory networks (GRN_modeler) and its 
            applications to synthetic biology.
  JOURNAL   Mol Syst Biol. 2025 Sep 29. doi: 10.1038/s44320-025-00148-8.
  PUBMED    41023501
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol. 2025 Sep 29. doi: 10.1038/s44320-025-00148-8."
COMMENT     Sequence Label: pJP_Bla01
FEATURES             Location/Qualifiers
     source          1..3373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(408..425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(542..1087)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(622..641)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1163..1182
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     terminator      complement(2465..2551)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2617..3150)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3339..3344,3345..3361,3362..3367))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gctcctggcg ttccacatcg ctcattttcg ccaaaagttg gcccagggct tcccggtatc
       61 aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc
      121 ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
      181 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
      241 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta
      301 ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct
      361 gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac
      421 tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat
      481 ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt
      541 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
      601 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc
      661 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt
      721 ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg
      781 cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      841 aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga
      901 gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg
      961 cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa
     1021 accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg
     1081 atctcaagaa gatcatctta ttaatcagat aaaatatttc taggctcatg agcccgaagt
     1141 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     1201 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctgctca tgtttgacag
     1261 cttatcattt ttttaggcta ccgatgtagc ctaacgcgat cgatttatga caacttgacg
     1321 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
     1381 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
     1441 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
     1501 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac
     1561 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
     1621 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
     1681 ttttccgttt cgcactggaa acccatgctg taccggtatt cccctgtttc atctcgcacc
     1741 ggaatcatcg tcaagaagtt gacaaaccgt tggccgttct tcttaaaatt gaccacctca
     1801 acctgcacct cggcgttcct atcaatcgct ttcctcatcg tattgatcgt gttggagtcg
     1861 acgtacttcc ttgtcgattt cggcttgacc attccgtcgg gtgactgaag aaaacggcag
     1921 tttctcccca agacctccgc attgctgtat cctgtcatat agagaaaagc ttccgaggcg
     1981 tacacaattg gcgtgtcttt ttgcttcagg tcgcacagaa tcagagcaac tgacgtgtca
     2041 acaggtccca gttctacttg ggggtttggc ctcttcataa tctgaatcag atagcccata
     2101 atgtcataac cgccgggagc gtagagcgta tgcatggtat atctccttct ctagtctcta
     2161 gtagctagca tatacctagg actgagctag tgtaaactct agaaatgcat aatgtgcctg
     2221 tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgagctcg cgccgattaa
     2281 atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt
     2341 cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg
     2401 gtctccccat gcgagagtag ggaactgcca ctaagtggtg ttcagttcgg ttctagggcg
     2461 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     2521 agtctttcga ctgagccttt cgttttattt gatgccgacg tccaatttac cgaacaactc
     2581 cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat
     2641 atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc
     2701 gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt
     2761 gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctctccgg agactgcgag
     2821 atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag
     2881 agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag
     2941 caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa
     3001 ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag ggccgagcct
     3061 acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc gactgccctg
     3121 ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     3181 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat aggctgtaca aaaaaacagt
     3241 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     3301 tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt
     3361 atgtcaagac gtc
//
