LOCUS       sequence-459320-        5118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0140h_3xC2 is an enhancer sequence, designed to drive 
            AAV-mediated transgene expression in striatal Pvalb interneurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP15481:
            pAAV-AiE0140h_3xC2-minBG-iCre(R297T)...
FEATURES             Location/Qualifiers
     source          1..5118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      826..835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             838..858
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2152..2263
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2381..2521
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2381..2510
                     /label=AAV2 ITR
     rep_origin      2596..3051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2683..2702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2893..2914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3068..3087)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3187..3209
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3247..3265)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3333..3437
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3438..3506,3507..4298)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3656..3675)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4469..5057
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4958..4977
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtccaaga aattagaata aagggaaaaa tgactttgag
      181 atgtactttc ttaaaataac tgaagtaatg accgcatgag ccagctgatg ggtttttaaa
      241 tggatacgtt tctatcagcc tgctgcttga tgccagagcc aaatatatat ggagtgtatt
      301 atatcatatc ctgtaggcag gagactgttg gggcattggg ggacccaaga aattagaata
      361 aagggaaaaa tgactttgag atgtactttc ttaaaataac tgaagtaatg accgcatgag
      421 ccagctgatg ggtttttaaa tggatacgtt tctatcagcc tgctgcttga tgccagagcc
      481 aaatatatat ggagtgtatt atatcatatc ctgtaggcag gagactgttg gggcattggg
      541 ggacccaaga aattagaata aagggaaaaa tgactttgag atgtactttc ttaaaataac
      601 tgaagtaatg accgcatgag ccagctgatg ggtttttaaa tggatacgtt tctatcagcc
      661 tgctgcttga tgccagagcc aaatatatat ggagtgtatt atatcatatc ctgtaggcag
      721 gagactgttg gggcattggg ggacgagctc gggctgggca taaaagtcag ggcagagcca
      781 tctattgctt acatttgctt ctgggatcca gatctttcga agctagccac catggtgccc
      841 aagaagaaga ggaaagtctc caacctgctg actgtgcacc aaaacctgcc tgccctccct
      901 gtggatgcca cctctgatga agtcaggaag aacctgatgg acatgttcag ggacaggcag
      961 gccttctctg aacacacctg gaagatgctc ctgtctgtgt gcagatcctg ggctgcctgg
     1021 tgcaagctga acaacaggaa atggttccct gctgaacctg aggatgtgag ggactacctc
     1081 ctgtacctgc aagccagagg cctggctgtg aagaccatcc aacagcacct gggccagctc
     1141 aacatgctgc acaggagatc tggcctgcct cgcccttctg actccaatgc tgtgtccctg
     1201 gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg agagagccaa gcaggccctg
     1261 gcctttgaac gcactgactt tgaccaagtc agatccctga tggagaactc tgacagatgc
     1321 caggacatca ggaacctggc cttcctgggc attgcctaca acaccctgct gcgcattgcc
     1381 gaaattgcca gaatcagagt gaaggacatc tcccgcaccg atggtgggag aatgctgatc
     1441 cacattggca ggaccaagac cctggtgtcc acagctggtg tggagaaggc cctgtccctg
     1501 ggggttacca agctggtgga gagatggatc tctgtgtctg gtgtggctga tgaccccaac
     1561 aactacctgt tctgccgggt cagaaagaat ggtgtggctg ccccttctgc cacctcccaa
     1621 ctgtccaccc gggccctgga agggatcttt gaggccaccc accgcctgat ctatggtgcc
     1681 aaggatgact ctgggcagag atacctggcc tggtctggcc actctgccag agtgggtgct
     1741 gccaccgaca tggccagggc tggtgtgtcc atccctgaaa tcatgcaggc tggtggctgg
     1801 accaatgtga acattgtgat gaactacatc agaaacctgg actctgagac tggggccatg
     1861 gtgaggctgc tcgaagatgg ggactgaggc gcgccgcggc cttaaagaga ccggttcact
     1921 gtgacagtaa aagagaccgg ttcactgtga gaatgaaaga gaccggttca ctgtgatcgg
     1981 aaaagagacc ggttcactgt gagcggcctt gaaacccagc agacaatgta gctcagtaga
     2041 aacccagcag acaatgtagc tgaatggaaa cccagcagac aatgtagctt cggagaaacc
     2101 cagcagacaa tgtagctgtc gacgaattca agcttctcga gagatcttcg actgtgcctt
     2161 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     2221 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     2281 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     2341 atagcaggca tgagatctca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     2401 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     2461 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     2521 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     2581 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2641 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     2701 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2761 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     2821 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     2881 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     2941 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     3001 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     3061 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     3121 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     3181 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     3241 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3301 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3361 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3421 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3481 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3541 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3601 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3661 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3721 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3781 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3841 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3901 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3961 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4021 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4081 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4141 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4201 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4261 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4321 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4381 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4441 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4501 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4561 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4621 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4681 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4741 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4801 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4861 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4921 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4981 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5041 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5101 ggccttttgc tcacatgt
//
