LOCUS       sequence-459390-        8746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single transcript cageIL-10 (mRens) + ER-renin (H2M).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Aldrete CA, Call CC, Sant'Anna LE, Vlahos AE, Pei J, Cong Q, Gao XJ
  TITLE     Orthogonalized human protease control of secreted signals.
  JOURNAL   Nat Chem Biol. 2025 Jan 15. doi: 10.1038/s41589-024-01831-x.
  PUBMED    39814991
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Jan 15. doi: 10.1038/s41589-024-01831-x."
COMMENT     Sequence Label: CA244
            CMVTO_ERret-H2M-Renin_T2A_mRens-cageIL-1...
FEATURES             Location/Qualifiers
     source          1..8746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(570..1361,1362..1430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1193..1212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1431..1535)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1610..1629)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1796..2175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2176..2379
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2330..2350
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2381..2399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2402..2420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2430..2454
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2546..2555
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3857..3877
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4001..4054
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5339..5368
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5369..5422
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5423..6133
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5568..5586)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5836..5855
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6140..6169
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6225..6242)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6231..6455
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6627..6646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6778..6825
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6833..7855
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6833..7855
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7985..8106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8022..8041)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8076..8095
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8155..8171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8155..8171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8168..8190)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8179..8195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8203..8209,8210..8227,8228..8233))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8248..8269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8386..8403)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8557..8746,1..399))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8637..8656)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
       61 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      241 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      481 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      541 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      601 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      661 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      721 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      781 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      841 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      901 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      961 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1021 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1081 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1141 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1201 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1261 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1321 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1381 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1441 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1501 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1561 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     1621 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     1681 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     1741 atctgcttag ggttaggcgt tttgcgctgc ttcgtacggg ccagatatac gcgttgacat
     1801 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1861 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1921 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1981 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2041 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2101 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2161 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     2221 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     2281 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     2341 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat
     2401 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
     2461 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
     2521 actctagcgt ttaaacttaa gcttcgccac catggacggt tggagaagga tgccccggtg
     2581 gggccttttg ctgctcctgt ggggaagctg caccttcggg gctagcctca ctctcggtaa
     2641 tactacttcc tcagtcatct taaccaacta catggatacg cagtattacg gggaaatcgg
     2701 cattggcaca ccccctcaga cttttaaggt agtttttgat actgggtcta gcaatgtttg
     2761 ggtccctagc agtaagtgtt cccgcctgta tacagcgtgt gtttaccata agttatttga
     2821 cgcctcagac tcctctagct ataaacacaa cggcaccgag ctcaccatcc actacagcac
     2881 agggactgtt tcggggtttc tgtcccagga cattattaca gtgggaggta ttacagtgac
     2941 ccaaatgttt ggggaggtga ccgagatgcc ggctctgccg ttcatgctgg cagagttcga
     3001 cggggtcgtt ggcatgggct tcatcgagca ggctgtgggg cgggtaaccc ccatcttcga
     3061 caacatcatt agccagggcg tgctaaagga ggacgtgttc agcttttatt acaatagaga
     3121 ttctgaaaac tctcagagtt tgggcggcca gatagtgctt ggaggcagtg atccccagca
     3181 ctacgaaggc aattttcact atataaatct gatcaaaact ggggtgtggc agatccagat
     3241 gaagggggtg tcagtgggtt cctctacgtt attgtgcgag gacggatgtc tggccgtggt
     3301 agacactgga gctagttaca tcagcggctc aaccagctca attgaaaagc tgatggaggc
     3361 actgggagca aaaaagcggt tgttcgatta cgtcgtgaaa tgtaatgagg gacctaccct
     3421 gcccgacatc tctttccatc tgggtggaaa agagtatact ctgacaagcg ccgattacgt
     3481 atttcaggag tcctactcgt ccaaaaaact ctgcacgtta gcaatccacg ccatggacat
     3541 ccctccacca accggaccta cctgggctct gggggccaca tttatacgaa aattctatac
     3601 cgagttcgat cgcaggaaca accgtatcgg ctttgcactt gccaggggcg ccgggacagg
     3661 agggtctggg ggtggcactg gcggatctgg aggagactac aaggacgatg acgacaacgg
     3721 cggatcgggg gggtctggtg gtagtggcgg ttcgggggga ggcacaggtg gatccatggc
     3781 tctcattgtc ttgggcggtg tggccgggtt actgctgttc atcggacttg gcatattttt
     3841 cgggggcggc tctctcgaga acttgtactt ccagagtggc agtggaggta cccttctcga
     3901 agccctgacc ttagccagtg cacggggacc cctgaggaag agatctgtgc caatggctaa
     3961 ggcgaaaccg aaattctcca tctcacctga cagcttgagc gaaggtcggg ggagtttgct
     4021 tacctgtggc gatgtcgagg agaatccagg acccatgcac tcctccgcgc tcttgtgttg
     4081 cctagtttta ttgaccggag tccgcgcatc acctgggcaa ggcactcaga gtgaaaattc
     4141 ttgcactcac ttccctggaa ttctccccaa catgctcaga gacctgcgag atgctttctc
     4201 tagagtcaaa acgttctttc aaatgaagga ccagctagat aatcttttgc tgaaagagtc
     4261 ccttctcgag gattttaaag gctatctggg ctgtcaggct cttagcgaga tgatacagtt
     4321 ctacctcgag gaggtgatgc cacaggctga gaaccaagac ccagacatta aagcccatgt
     4381 aatctcactg ggggaaaacc tgaacacatt acgcctgcgg ctgcggaggt gccataggct
     4441 gctgccttgt gaaaatggtg ggggcagtgg aggcaagagc aaggccgtgg aacaggtgaa
     4501 gaatgccttt aataagctgc aggaaaaggg gatctacaag gcaatgagcg agtttgatat
     4561 attcatcaac tatatcgagg cctacatgac catgaaaatt cgtaacggcg cctacatcca
     4621 tcccttccac ctgctctacc acaacaagag cggcggtggg ggatctggtg gagggggatc
     4681 aggaggtggc ggatcccatg gcactgagct tccatctcct ccttctgtat ggttcgaagc
     4741 agagttcttc catcacattc tgcattggac tcccattcca aaccaatcgg agagcacctg
     4801 ctacgaagtg gctctgttaa gatacggcat tgagtcttgg aacagcatca gcaactgctc
     4861 acaaacgctc tcttatgatt tgaccgcggt gacacttgac ctctaccact caaacggcta
     4921 tagagcccgg gttcgagccg tggatggctc ccgacactcc aactggaccg tcactaatac
     4981 acggtttagc gtggacgagg tgaccctgac agtgggaagt gtgaacctcg aaattcataa
     5041 cgggtttatt ctgggaaaaa tacagctgcc tcgtccaaag atggcaccag ccaacgacac
     5101 gtacgaaagc attttcagcc acttccggga gtacgaaatc gcaatccgca aggtaccggg
     5161 taattttact tttacccaca agaaggttaa gcacgaaaac ttcagcttgt taacctctgg
     5221 cgaggtcgga gagttttgcg tgcaagtgaa gcctagcgtc gcttcacgct ccaataaagg
     5281 tatgtggagt aaagaggagt gcatcagtct cacaaggcag tattttacgg tcaccaatga
     5341 gcagaagctc atcagcgaag aggacctgga gggacgggga agtttgctta cctgtggcga
     5401 tgtcgaggag aatccaggtc ccatggtgag caagggcgag gaggataaca tggccatcat
     5461 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     5521 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     5581 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     5641 ctccaaggcc tacgtgaagc accccgccga cattcccgac tacttgaagc tgtccttccc
     5701 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     5761 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     5821 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     5881 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     5941 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     6001 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     6061 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     6121 gctgtacaag tagaggcccg aacaaaaact catctcagaa gaggatctga atagcgccgt
     6181 cgaccatcat cataatcatc attgagttta aacccgctga tcagcctcga ctgtgccttc
     6241 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6301 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6361 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6421 tagcaggcat gctggggatg cggtgggctc tatggagatc tatcgatgtt ctggggatgc
     6481 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     6541 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6601 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6661 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6721 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtacctagaa
     6781 gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg ccaaaaagcc
     6841 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     6901 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     6961 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     7021 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     7081 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     7141 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     7201 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     7261 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     7321 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     7381 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     7441 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     7501 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     7561 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     7621 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     7681 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     7741 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     7801 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt
     7861 actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7921 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7981 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8041 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8101 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8161 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8221 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8281 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8341 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8401 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8461 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8521 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8581 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8641 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8701 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctt
//
