LOCUS       sequence-459396-        9485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single transcript cageIL-10 (mRens) + cleaveIL-6 (mRens) + ER-renin 
            (H2M).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Aldrete CA, Call CC, Sant'Anna LE, Vlahos AE, Pei J, Cong Q, Gao XJ
  TITLE     Orthogonalized human protease control of secreted signals.
  JOURNAL   Nat Chem Biol. 2025 Jan 15. doi: 10.1038/s41589-024-01831-x.
  PUBMED    39814991
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Jan 15. doi: 10.1038/s41589-024-01831-x."
COMMENT     Sequence Label: CA245
            CMVTO_ERret-H2M-Renin_P2A_mRens-hIL6-ren...
FEATURES             Location/Qualifiers
     source          1..9485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(570..1361,1362..1430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1193..1212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1431..1535)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1610..1629)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1796..2175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2176..2379
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2330..2350
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2381..2399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2402..2420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2430..2454
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2545..2554
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3856..3876
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4009..4065
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4741..4794
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6079..6108
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6109..6162
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6163..6873
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6308..6326)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6576..6595
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6879..6908
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6964..6981)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6970..7194
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7366..7385)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7517..7564
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7572..8594
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7572..8594
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8724..8845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8761..8780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8815..8834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8894..8910)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8894..8910)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8907..8929)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8918..8934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8942..8948,8949..8966,8967..8972))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8987..9008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9125..9142)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9296..9485,1..399))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9376..9395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
       61 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      241 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      481 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      541 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      601 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      661 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      721 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      781 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      841 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      901 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      961 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1021 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1081 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1141 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1201 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1261 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1321 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1381 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1441 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1501 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1561 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     1621 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     1681 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     1741 atctgcttag ggttaggcgt tttgcgctgc ttcgtacggg ccagatatac gcgttgacat
     1801 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1861 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1921 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1981 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2041 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2101 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2161 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     2221 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     2281 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     2341 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat
     2401 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
     2461 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
     2521 actctagcgt ttaaacttaa gcttgccacc atggacggtt ggagaaggat gccccggtgg
     2581 ggccttttgc tgctcctgtg gggaagctgc accttcgggg ctagcctcac tctcggtaat
     2641 actacttcct cagtcatctt aaccaactac atggatacgc agtattacgg ggaaatcggc
     2701 attggcacac cccctcagac ttttaaggta gtttttgata ctgggtctag caatgtttgg
     2761 gtccctagca gtaagtgttc ccgcctgtat acagcgtgtg tttaccataa gttatttgac
     2821 gcctcagact cctctagcta taaacacaac ggcaccgagc tcaccatcca ctacagcaca
     2881 gggactgttt cggggtttct gtcccaggac attattacag tgggaggtat tacagtgacc
     2941 caaatgtttg gggaggtgac cgagatgccg gctctgccgt tcatgctggc agagttcgac
     3001 ggggtcgttg gcatgggctt catcgagcag gctgtggggc gggtaacccc catcttcgac
     3061 aacatcatta gccagggcgt gctaaaggag gacgtgttca gcttttatta caatagagat
     3121 tctgaaaact ctcagagttt gggcggccag atagtgcttg gaggcagtga tccccagcac
     3181 tacgaaggca attttcacta tataaatctg atcaaaactg gggtgtggca gatccagatg
     3241 aagggggtgt cagtgggttc ctctacgtta ttgtgcgagg acggatgtct ggccgtggta
     3301 gacactggag ctagttacat cagcggctca accagctcaa ttgaaaagct gatggaggca
     3361 ctgggagcaa aaaagcggtt gttcgattac gtcgtgaaat gtaatgaggg acctaccctg
     3421 cccgacatct ctttccatct gggtggaaaa gagtatactc tgacaagcgc cgattacgta
     3481 tttcaggagt cctactcgtc caaaaaactc tgcacgttag caatccacgc catggacatc
     3541 cctccaccaa ccggacctac ctgggctctg ggggccacat ttatacgaaa attctatacc
     3601 gagttcgatc gcaggaacaa ccgtatcggc tttgcacttg ccaggggcgc cgggacagga
     3661 gggtctgggg gtggcactgg cggatctgga ggagactaca aggacgatga cgacaacggc
     3721 ggatcggggg ggtctggtgg tagtggcggt tcggggggag gcacaggtgg atccatggct
     3781 ctcattgtct tgggcggtgt ggccgggtta ctgctgttca tcggacttgg catatttttc
     3841 gggggcggct ctctcgagaa cttgtacttc cagagtggca gtggaggtac ccttctcgaa
     3901 gccctgacct tagccagtgc acggggaccc ctgaggaaga gatctgtgcc aatggctaag
     3961 gcgaaaccga aattctccat ctcacctgac agcttgagcg gaagcggagc tactaacttc
     4021 agcctgctga agcaggctgg cgacgtggaa gaaaacccag gacctatgaa cagcttctct
     4081 accagcgctt ttggacccgt ggccttttcg ttgggcctgc ttctcgttct tcctgccgca
     4141 ttccctgcac cagtcccccc aggcgaagat tctaaagatg tggctgctcc acacagacag
     4201 ccccttacta gcagtgaacg tattgataaa cagatcaggt acatcctgga cggaatctca
     4261 gccctaagaa aagagacatg caataagtcc aacatgtgtg agagttccaa ggaggccctc
     4321 gccgagaata acctgaatct acccaagatg gccgagaagg acggttgttt ccagagtggg
     4381 tttaacgaag agacttgcct ggtgaaaatt ataaccgggc tgctggaatt cgaagtgtac
     4441 ctggagtatt tgcaaaatcg gttcgagtca tctgaagagc aggccagggc tgtccagatg
     4501 tctaccaaag tattaatcca gttcttgcag aagaaagcta agaacctgga tgcatacatc
     4561 catcccttcc acctgctcta ccacaacaag agcataacaa ctcctgaccc caccacaaac
     4621 gcgagcctgc ttacaaagct ccaggcgcag aatcagtggt tacaagacat gacgacccat
     4681 ctgattctcc gatcatttaa ggaatttttg caatcctccc tccgggcact gcgccaaatg
     4741 gaaggtcggg ggagtttgct tacctgtggc gatgtcgagg agaatccagg acccatgcac
     4801 tcctccgcgc tcttgtgttg cctagtttta ttgaccggag tccgcgcatc acctgggcaa
     4861 ggcactcaga gtgaaaattc ttgcactcac ttccctggaa ttctccccaa catgctcaga
     4921 gacctgcgag atgctttctc tagagtcaaa acgttctttc aaatgaagga ccagctagat
     4981 aatcttttgc tgaaagagtc ccttctcgag gattttaaag gctatctggg ctgtcaggct
     5041 cttagcgaga tgatacagtt ctacctcgag gaggtgatgc cacaggctga gaaccaagac
     5101 ccagacatta aagcccatgt aatctcactg ggggaaaacc tgaacacatt acgcctgcgg
     5161 ctgcggaggt gccataggct gctgccttgt gaaaatggtg ggggcagtgg aggcaagagc
     5221 aaggccgtgg aacaggtgaa gaatgccttt aataagctgc aggaaaaggg gatctacaag
     5281 gcaatgagcg agtttgatat attcatcaac tatatcgagg cctacatgac catgaaaatt
     5341 cgtaacggcg cctacatcca tcccttccac ctgctctacc acaacaagag cggcggtggg
     5401 ggatctggtg gagggggatc aggaggtggc ggatcccatg gcactgagct tccatctcct
     5461 ccttctgtat ggttcgaagc agagttcttc catcacattc tgcattggac tcccattcca
     5521 aaccaatcgg agagcacctg ctacgaagtg gctctgttaa gatacggcat tgagtcttgg
     5581 aacagcatca gcaactgctc acaaacgctc tcttatgatt tgaccgcggt gacacttgac
     5641 ctctaccact caaacggcta tagagcccgg gttcgagccg tggatggctc ccgacactcc
     5701 aactggaccg tcactaatac acggtttagc gtggacgagg tgaccctgac agtgggaagt
     5761 gtgaacctcg aaattcataa cgggtttatt ctgggaaaaa tacagctgcc tcgtccaaag
     5821 atggcaccag ccaacgacac gtacgaaagc attttcagcc acttccggga gtacgaaatc
     5881 gcaatccgca aggtaccggg taattttact tttacccaca agaaggttaa gcacgaaaac
     5941 ttcagcttgt taacctctgg cgaggtcgga gagttttgcg tgcaagtgaa gcctagcgtc
     6001 gcttcacgct ccaataaagg tatgtggagt aaagaggagt gcatcagtct cacaaggcag
     6061 tattttacgg tcaccaatga gcagaagctc atcagcgaag aggacctgga gggacgggga
     6121 agtttgctta cctgtggcga tgtcgaggag aatccaggtc ccatggtgag caagggcgag
     6181 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     6241 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     6301 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     6361 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga cattcccgac
     6421 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     6481 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     6541 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     6601 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     6661 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     6721 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     6781 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     6841 tccaccggcg gcatggacga gctgtacaag tagggcccga acaaaaactc atctcagaag
     6901 aggatctgaa tagcgccgtc gaccatcatc ataatcatca ttgagtttaa acccgctgat
     6961 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     7021 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     7081 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     7141 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggagatct
     7201 atcgatgttc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     7261 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7321 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7381 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7441 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7501 atggttcacg tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa
     7561 cttccttggc caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     7621 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     7681 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     7741 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     7801 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     7861 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     7921 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     7981 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     8041 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     8101 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     8161 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     8221 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     8281 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     8341 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     8401 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     8461 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     8521 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     8581 gggcaaagga atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     8641 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     8701 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     8761 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8821 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     8881 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8941 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     9001 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     9061 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     9121 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     9181 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9241 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9301 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9361 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9421 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9481 cgctt
//
