LOCUS       sequence-459405-        9837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Kerby RL, Youn H, Roberts GP
  TITLE     RcoM: a new single-component transcriptional regulator of CO 
            metabolism in bacteria.
  JOURNAL   J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7.
  PUBMED    18326575
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "2008-05"; volume: "190(9)"; pages: "3336-43. 
            Epub 2008 Mar 7"
COMMENT     Sequence Label: UQ4876
FEATURES             Location/Qualifiers
     source          1..9837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(834..1916)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1878..1897
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1917..1994)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(2513..2518,2519..2534,2535..2541)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2549..2565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2554..2576
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(2738..2757)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2749..3564
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2749..3564
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2822..2841)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(4012..4060)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(4225..7272)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     7248..7270
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7262..7279
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7263..7279
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(8608..8635)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        8702..8806
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8807..8875,8876..9667)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9025..9044)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagccgaac gccagcaaga
      781 cgtagcccag cgcgtcggcc aattcgcgct aacttacatt aattgcgttg cgctcactgc
      841 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      901 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
      961 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     1021 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     1081 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     1141 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     1201 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     1261 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     1321 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     1381 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     1441 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     1501 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     1561 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     1621 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     1681 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     1741 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     1801 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     1861 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     1921 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac
     1981 cattcgatgg tgtcaacgta aatgcatgcc gcttcgcctt cgcgcgcgaa ttggccgcca
     2041 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg
     2101 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc
     2161 tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga
     2221 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc
     2281 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccg ggcttatcga
     2341 ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt
     2401 gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat
     2461 gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat
     2521 taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2581 agaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggggg ggggggggaa
     2641 agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     2701 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     2761 caacgggaaa cgtcttgctc gaggccgcga ttaaattcca acatggatgc tgatttatat
     2821 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat
     2881 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2941 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     3001 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa
     3061 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     3121 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     3181 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     3241 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag
     3301 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     3361 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     3421 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     3481 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     3541 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     3601 attacgctga cttgacggga cggcggcttt gttgaataaa tcgaactttt gctgagttga
     3661 aggatcagat cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca
     3721 aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct
     3781 ggctggatga tggggcgatt caggcctggt atgagtcagc aacaccttct tcacgaggca
     3841 gacctcagcg ccccccccca attccccgga tctaaagttt tgtcgtcttt ccagacgtta
     3901 gtaaatgaat tttctgtatg aggttttgct aaacaacttt caacagtttc agcggagtga
     3961 gaatagaaag gaacaactaa aggaattgcg aataataatt ttttcacgtt gaaaatctcc
     4021 aaaaaaaaag gctccaaaag gagcctttaa ttgtatcggt ttatcagctt gctttcgagg
     4081 tgaatttctt aaacagcttg ataccgatag ttgcgccgac aatgacaaca accatcgccc
     4141 acgcataacc gatatattcg gtcgctgagg cttgcaggga gtcaaaggcc gcttttgcgg
     4201 gatcgatccc ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg
     4261 accggcgctc agctggaatt ccgccgatac tgacgggctc caggagtcgt cgccaccaat
     4321 ccccatatgg aaaccgtcga tattcagcca tgtgccttct tccgcgtgca gcagatggcg
     4381 atggctggtt tccatcagtt gctgttgact gtagcggctg atgttgaact ggaagtcgcc
     4441 gcgccactgg tgtgggccat aattcaattc gcgcgtcccg cagcgcagac cgttttcgct
     4501 cgggaagacg tacggggtat acatgtctga caatggcaga tcccagcggt caaaacaggc
     4561 ggcagtaagg cggtcgggat agttttcttg cggccctaat ccgagccagt ttacccgctc
     4621 tgctacctgc gccagctggc agttcaggcc aatccgcgcc ggatgcggtg tatcgctcgc
     4681 cacttcaaca tcaacggtaa tcgccatttg accactacca tcaatccggt aggttttccg
     4741 gctgataaat aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa tcagcaccgc
     4801 atcagcaagt gtatctgccg tgcactgcaa caacgctgct tcggcctggt aatggcccgc
     4861 cgccttccag cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac ttacgccaat
     4921 gtcgttatcc agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca gttgtttttt
     4981 atcgccaatc cacatctgtg aaagaaagcc tgactggcgg ttaaattgcc aacgcttatt
     5041 acccagctcg atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg cgtgggacgc
     5101 ggcggggagc gtcacactga ggttttccgc cagacgccac tgctgccagg cgctgatgtg
     5161 cccggcttct gaccatgcgg tcgcgttcgg ttgcactacg cgtactgtga gccagagttg
     5221 cccggcgctc tccggctgcg gtagttcagg cagttcaatc aactgtttac cttgtggagc
     5281 gacatccaga ggcacttcac cgcttgccag cggcttacca tccagcgcca ccatccagtg
     5341 caggagctcg ttatcgctat gacggaacag gtattcgctg gtcacttcga tggtttgccc
     5401 ggataaacgg aactggaaaa actgctgctg gtgttttgct tccgtcagcg ctggatgcgg
     5461 cgtgcggtcg gcaaagacca gaccgttcat acagaactgg cgatcgttcg gcgtatcgcc
     5521 aaaatcaccg ccgtaagccg accacgggtt gccgttttca tcatatttaa tcagcgactg
     5581 atccacccag tcccagacga agccgccctg taaacgggga tactgacgaa acgcctgcca
     5641 gtatttagcg aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc aaaggatcag
     5701 cgggcgcgtc tctccaggta gcgaaagcca ttttttgatg gaccatttcg gcacagccgg
     5761 gaagggctgg tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg tggccgtggt
     5821 gtcggctccg ccgccttcat actgcaccgg gcgggaagga tcgacagatt tgatccagcg
     5881 atacagcgcg tcgtgattag cgccgtggcc tgattcattc cccagcgacc agatgatcac
     5941 actcgggtga ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca tcgccggtag
     6001 ccagcgcgga tcatcggtca gacgattcat tggcaccatg ccgtgggttt caatattggc
     6061 ttcatccacc acatacaggc cgtagcggtc gcacagcgtg taccacagcg gatggttcgg
     6121 ataatgcgaa cagcgcacgg cgttaaagtt gttctgcttc atcagcagga tatcctgcac
     6181 catcgtctgc tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc
     6241 tcgaatcagc aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg
     6301 gaaaccgaca tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac
     6361 caccgcacga tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag
     6421 acgtagtgtg acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg
     6481 cgcggtgccg ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta
     6541 gtcacgcaac tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc
     6601 attaaagcga gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga
     6661 gacgtcacgg aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc
     6721 actccagcgc agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag
     6781 gtcaaattca gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca
     6841 cagatgaaac gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca
     6901 gctttcatca acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa
     6961 cggcggattg accgtaatgg gataggtcac gttggtgtag atgggcgcat cgtaaccgtg
     7021 catctgccag tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca
     7081 gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct
     7141 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     7201 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     7261 ttgtaaaacg acggccagtg aattccccgg aaagcttggc gtaatcatgg tcatagctgt
     7321 ttcctctcta gccccccctg caggtcgacg gatcacactg gcctcctttg tcgtcaaagt
     7381 cgcacggccg gaaacgtcac gggcctttaa gcaaggattt cacaatgcaa tgcagcaatt
     7441 caacgcaatt tcccgaacgg cgggttcacg cgcgccaact agcggaaaac gtacatgaaa
     7501 cccgaaccgg ccggctggtg ccgccgtgac agcgcctgtt cgctcgcgcg cggcggacag
     7561 cacgattgcg cgacgcaacg ggccctttca tgccggtttc ccgaacggcg ggtttacctg
     7621 cgtgaacgac ccggaaattt gcgcgaaaat cggtagatag cgatactgca agataaaaat
     7681 cgcctgagag cgtggacagc cccatgagga caggagagcc cgcatgaaat cgtctgagcc
     7741 cgcttccgta tcggcggccg agcgccgcgc ggagacgttc cagcacaagc tcgagcagtt
     7801 caatccgggg atcgtctggc tggaccagca cggccgcgtc acggcattca acgacgtcgc
     7861 gctgcaaatt ctcgggccgg ccggcgagca atcgctaggc gtggcgcagg atagtctgtt
     7921 cggcatcgac gtggtgcaac tgcacccgga gaaaagccgc gacaagctgc gcttcctgct
     7981 gcagtcgaaa gacgtgggtg gctgcccggt caagtcgccg ccgccggtgg cgatgatgat
     8041 caatattccc gaccggatcc tgatgatcaa ggtgtccagc atgatcgccg ccggcggcgc
     8101 gtgcggcacc tgcatgatct tctacgacgt caccgatctg accaccgagc cctccggcct
     8161 gccggcgggc ggcagcgcgc cctcgccgag gcgcctcttc aagattcccg tgtaccggaa
     8221 gaaccgcgtg atcctgctcg atctgaaaga catcgtgcgc tttcagggcg acggccacta
     8281 cacgacgatc gtgacgcggg acgaccgcta cctgtccaat ctttcactgg ccgatctgga
     8341 gctgcgtctc gacagcagca tttatctgcg cgttcatcgc agccatatcg tgagcctgca
     8401 atatgcggtg gagctggtca aactggacga gagcgtcaat ctcgtcatgg acgacgcgga
     8461 acagacccag gtgccggtca gccgctcgcg aacggcgcaa ctgaaggagc ttctcggcgt
     8521 ggtttgagaa ggtgtaagct tggctgtttt ggcggatgag agaagaaatt cgtcgcccgc
     8581 cataaactgc caggcatcaa attaagcaga aggccatccg tcaggatggc cttctgctta
     8641 atttgatgcc tggcagttta tggcgggcga cgaatcaggt ggcacttttc ggggaaatgt
     8701 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8761 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8821 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8881 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8941 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9001 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9061 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9121 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9181 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9241 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9301 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9361 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9421 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9481 tggctggttt attgctgata aatctggcgc cggtgagcgt gggtctcgcg gtatcattgc
     9541 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9601 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9661 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9721 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9781 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
