LOCUS       sequence-459408-       14780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14780)
  AUTHORS   Aldrete CA, Call CC, Sant'Anna LE, Vlahos AE, Pei J, Cong Q, Gao XJ
  TITLE     Orthogonalized human protease control of secreted signals.
  JOURNAL   Nat Chem Biol. 2025 Jan 15. doi: 10.1038/s41589-024-01831-x.
  PUBMED    39814991
REFERENCE   2  (bases 1 to 14780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Jan 15. doi: 10.1038/s41589-024-01831-x."
COMMENT     Sequence Label: CA-0248
            EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2...
FEATURES             Location/Qualifiers
     source          1..14780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(830..850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        835..1164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1015..1150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2298..2363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2493..2513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2938..3072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2975..2994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3029..3048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3240..3873
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3920..4045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4538..4771
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4956..5000
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5275..5486
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5487..6425
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6382..6402
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7622..7651
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7652..7705
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9011..9031
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             9155..9208
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9209..9919
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9354..9372)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9622..9641
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    9934..10522
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9987..10007)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10405..10416)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10727..11360
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(12839..12857)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12840..12857)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(13147..13166)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(13380..13399)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13499..13521
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(13559..13577)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13645..13749
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13750..13818,13819..14610)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13968..13987)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
      841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1141 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     1201 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1261 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     1321 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     1381 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     1441 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     1501 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     1561 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     1621 attgatgacc tggtggatac gggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     1681 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     1741 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     1801 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     1861 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     1921 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     1981 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     2041 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     2101 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     2161 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     2221 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     2281 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     2341 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     2401 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     2461 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     2521 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     2581 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     2641 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     2701 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     2761 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     2821 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2881 aacctcccac acctccccct gaacctgaaa cataaaatga atgcatttgt tgttgttaac
     2941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3061 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     3121 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     3181 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     3241 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     3301 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     3361 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     3421 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3481 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     3541 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     3601 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     3661 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     3721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     3901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     3961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     4201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     4261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     4321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     4381 agccgccgct gatcttcaga cctggaggag gagatatgag ggacatttgg agaagtgaat
     4441 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     4501 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     4561 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     4621 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     4681 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     4741 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     4801 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     4861 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     4921 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     4981 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     5041 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     5101 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     5161 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     5221 acagatccat tcgattagtg aacggatctc gacggtatcg atgtgcccgt cagtgggcag
     5281 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     5341 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     5401 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     5461 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     5521 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     5581 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     5641 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     5701 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     5761 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     5821 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     5881 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     5941 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     6001 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     6061 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     6121 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     6181 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgcgcttt tggagtacgt
     6241 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     6301 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     6361 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     6421 ttcaggtgtc gtgagctagc tcgccaccat gtacaggatg caactcctgt cttgcattgc
     6481 actaagtctt gcacttgtca caaacgctag cgctccaacc tccagctcta caaaaaagac
     6541 tcagctgcag ctggagcacc tgctgctcga cctacaaatg atactcaacg gaatcaacaa
     6601 ctataagaat cctaagctga cgcggatgct gaccgccaag ttttacatgc ccaaaaaggc
     6661 gaccgagctg aagcatttgc agtgtctgga ggaagagctc aagcctttgg aggaggtgct
     6721 taatctcgcc caaagcaaaa atttccactt tgatccgcgg gacgtcgtgt caaatatcaa
     6781 cgtctttgtg ctggaactga aaggctctga gacaaccttt atgtgtgaat atgccgatga
     6841 aactgctacc atcgttgagt ttttaaatcg atggataaca ttttgccaga gtataatttc
     6901 tactttgacc ggcgccggga caggatacat ccatcccttc cacctgctct accacaacaa
     6961 gagcggcggt gggggatccg cggtgaatgg cacttcccag ttcacatgct tctacaattc
     7021 gagagccaac atctcctgtg tctggagcca agatggggct ctgcaggaca cttcctgcca
     7081 agtccatgcc tggccggaca gacggcggtg gaaccaaacc tgtgagctgc tccccgtgag
     7141 tcaagcatcc tgggcctgca acctgatcct cggagcccca gattctcaga aactgaccac
     7201 agttgacatc gtcaccctga gggtgctgtg ccgtgagggg gtgcgatgga gggtgatggc
     7261 catccaggac ttcaagccct ttgagaacct tcgcctgatg gcccccatct ccctccaagt
     7321 tgtccacgtg gagacccaca gatgcaacat aagctgggaa atctcccaag cctcccacta
     7381 ctttgaaaga cacctggagt tcgaggcccg gacgctgtcc ccaggccaca cctgggagga
     7441 ggcccccctg ctgactctca agcagaagca ggaatggatc tgcctggaga cgctcacccc
     7501 agacacccag tatgagtttc aggtgcgggt caagcctctg caaggcgagt tcacgacctg
     7561 gagcccctgg agccagcccc tggccttcag gacaaagcct gcagcccttg ggaaggacac
     7621 cgagcagaag ctcatcagcg aagaggacct ggaaggtcgg gggagtttgc ttacctgtgg
     7681 cgatgtcgag gagaatccag gacccatgga cggttggaga aggatgcccc ggtggggcct
     7741 tttgctgctc ctgtggggaa gctgcacctt cggggctagc ctcactctcg gtaatactac
     7801 ttcctcagtc atcttaacca actacatgga tacgcagtat tacggggaaa tcggcattgg
     7861 cacaccccct cagactttta aggtagtttt tgatactggg tctagcaatg tttgggtccc
     7921 tagcagtaag tgttcccgcc tgtatacagc gtgtgtttac cataagttat ttgacgcctc
     7981 agactcctct agctataaac acaacggcac cgagctcacc atccactaca gcacagggac
     8041 tgtttcgggg tttctgtccc aggacattat tacagtggga ggtattacag tgacccaaat
     8101 gtttggggag gtgaccgaga tgccggctct gccgttcatg ctggcagagt tcgacggggt
     8161 cgttggcatg ggcttcatcg agcaggctgt ggggcgggta acccccatct tcgacaacat
     8221 cattagccag ggcgtgctaa aggaggacgt gttcagcttt tattacaata gagattctga
     8281 aaactctcag agtttgggcg gccagatagt gcttggaggc agtgatcccc agcactacga
     8341 aggcaatttt cactatataa atctgatcaa aactggggtg tggcagatcc agatgaaggg
     8401 ggtgtcagtg ggttcctcta cgttattgtg cgaggacgga tgtctggccg tggtagacac
     8461 tggagctagt tacatcagcg gctcaaccag ctcaattgaa aagctgatgg aggcactggg
     8521 agcaaaaaag cggttgttcg attacgtcgt gaaatgtaat gagggaccta ccctgcccga
     8581 catctctttc catctgggtg gaaaagagta tactctgaca agcgccgatt acgtatttca
     8641 ggagtcctac tcgtccaaaa aactctgcac gttagcaatc cacgccatgg acatccctcc
     8701 accaaccgga cctacctggg ctctgggggc cacatttata cgaaaattct ataccgagtt
     8761 cgatcgcagg aacaaccgta tcggctttgc acttgccagg ggcgccggga caggagggtc
     8821 tgggggtggc actggcggat ctggaggaga ctacaaggac gatgacgaca acggcggatc
     8881 gggggggtct ggtggtagtg gcggttcggg gggaggcaca ggtggatcca tggctctcat
     8941 tgtcttgggc ggtgtggccg ggttactgct gttcatcgga cttggcatat ttttcggggg
     9001 cggctctctc gagaacttgt acttccagag tggcagtgga ggtacccttc tcgaagccct
     9061 gaccttagcc agtgcacggg gacccctgag gaagagatct gtgccaatgg ctaaggcgaa
     9121 accgaaattc tccatctcac ctgacagctt gagcgaggga cggggaagtt tgcttacctg
     9181 tggcgatgtc gaggagaatc caggtcccat ggtgagcaag ggcgaggagg ataacatggc
     9241 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     9301 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     9361 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     9421 gtacggctcc aaggcctacg tgaagcaccc cgccgacatt cccgactact tgaagctgtc
     9481 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     9541 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     9601 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     9661 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     9721 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     9781 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     9841 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     9901 ggacgagctg tacaagtagt acgaattccc gataatcaac ctctggatta caaaatttgt
     9961 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
    10021 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
    10081 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
    10141 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
    10201 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
    10261 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
    10321 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
    10381 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
    10441 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
    10501 tccctttggg ccgcctcccc gcatcgggat ttctgcagtc gagacctaga aaaacatgga
    10561 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
    10621 gaggaggagg aggtgggttt tccagtcaca cctcaggttc ctttaagacc aatgacttac
    10681 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
    10741 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
    10801 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
    10861 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
    10921 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
    10981 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggag
    11041 tacttcaaga actgctgaca tcgagcttgc tacaagggac tttccgctgg ggactttcca
    11101 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatgctg catataagca
    11161 gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    11221 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    11281 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    11341 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
    11401 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tataatagat ttagcaggaa
    11461 ttgaactagg agtggagcac acaggcaaag ctgcagaagt acttggaaga agccaccaga
    11521 gatactcacg attctgcaca tacctggcta atcccagatc ctaaggatta cattaagttt
    11581 actaacattt atataatgat ttatagttta aagtataaac ttatctaatt tactattctg
    11641 acagatatta attaatcctc aaatatcata agagatgatt actattatcc ccatttaaca
    11701 caagaggaaa ctgagaggga aagatgttga agtaattttc ccacaattac agcatccgtt
    11761 agttacgact ctatgatctt ctgacacaaa ttccatttac tcctcaccct atgactcagt
    11821 cgaatatatc aaagttatgg acattatgct aagtaacaaa ttaccctttt atatagtaaa
    11881 tactgagtag attgagagaa gaaattgttt gcaaacctga atagcttcaa gaagaagaga
    11941 agtgaggata agaataacag ttgtcattta acaagtttta acaagtaact tggttagaaa
    12001 gggattcaaa tgcataaagc aagggataaa tttttctggc aacaagacta tacaatataa
    12061 ccttaaatat gacttcaaat aattgttgga acttgataaa actaattaaa tattattgaa
    12121 gattatcaat attataaatg taatttactt ttaaaaaggg aacatagaaa tgtgtatcat
    12181 tagagtagaa aacaatcctt attatcacaa tttgtcaaaa caagtttgtt attaacacaa
    12241 gtagaatact gcattcaatt aagttgactg cagattttgt gttttgttaa aattagaaag
    12301 agataacaac aatttgaatt attgaaagta acatgtaaat agttctacat acgttctttt
    12361 gacatcttgt tcaatcattg atcgaagttc tttatcttgg aagaatttgt tccaaagact
    12421 ctgaaataag gaaaacaatc tattatatag tctcacacct ttgttttact tttagtgatt
    12481 tcaatttaat aatgtaaatg gttaaaattt attcttctct gagatcattt cacattgcag
    12541 atagaaaacc tgagactggg gtaattttta ttaaaatcta atttaatctc agaaacacat
    12601 ctttattcta acatcaattt ttccagtttg atattatcat ataaagtcag ccttcctcat
    12661 ctgcaggttc cacaacaaaa atccaaccaa ctgtggatca aaaatattgg gaaaaaatta
    12721 aaaatagcaa tacaacaata aaaaaataca aatcagaaaa acaggacagt ataacaactt
    12781 tatttagcat ttacaatcta ttaggtatta taagtaatct agaattaatt ccgtgtattc
    12841 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
    12901 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
    12961 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
    13021 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcggta
    13081 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
    13141 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
    13201 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
    13261 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
    13321 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
    13381 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
    13441 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
    13501 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
    13561 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
    13621 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    13681 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
    13741 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
    13801 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
    13861 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
    13921 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
    13981 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    14041 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    14101 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    14161 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    14221 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    14281 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    14341 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    14401 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    14461 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    14521 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    14581 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    14641 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    14701 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    14761 gaaaagatca aaggatcttc
//
