LOCUS       sequence-459416-        1294 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of p300 KAT enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Dewing SM, Showalter SA
  TITLE     Probing Enzymatic Acetylation Events in Real Time With NMR 
            Spectroscopy: Insights Into Acyl-Cofactor Dependent p300 
            Modification of Histone H4.
  JOURNAL   Proteins. 2025 Jun 1. doi: 10.1002/prot.26848.
  PUBMED    40452297
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2025 Jun 1. doi: 10.1002/prot.26848."
COMMENT     Sequence Label: p300 KAT LIC 1M
FEATURES             Location/Qualifiers
     source          1..1294
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      363..372
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gap             1240..1253
                     /estimated_length=14
     gap             1274..1293
                     /estimated_length=20
ORIGIN
        1 ctnctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcaagc ttgtcgacgg cgctcgaatt cggatccgtt atccacttcc aatgttatta
      121 ggtgtagaca aagcggtcct ggctctgcgt gtgcagctcc accagcatgc acatggtgga
      181 ccactgggct cttcggagtg aagagaactc caggtgcttg tcccttgcca gcgtgagaaa
      241 cgcatcccga ccatccatca gatcgcaggg gatgagagga tcaggatcaa caatgggagg
      301 cagggagttg gcagcagggc cagcaatgag gcggatcaca aagaagacct ctttatgctt
      361 ctccatggtg gcatatagtt tctgtgagag gtcgttagat acattgggca tcccgggttt
      421 cttcttgttg cccctactca ggctgctctt atttttgctg gttttcttat tattcttctt
      481 tttagcattt ttgctgtctc ccttggtcac atctgtgctt tcattgctgg tgttttcctc
      541 tcgttttctc tcttcttcct cctgttccag ttccttaatg ctttcttcca gaacattggg
      601 ccagaaatta ccctcgaaat aaggcaattc ctttgcactt gttaatctat cttcagtagc
      661 ttgtttaaaa atatccttgt agtcatggac aatacgctct gatacagcct tgtcaagcat
      721 ttttttgtac cattcctgca gtcgcttggg cttgggtatc ttctggtcag gaggatggca
      781 atggaagata taatcatctc cctcacttgg tggacatgcc caaatatgcc ctgttgtgta
      841 acctaatttc ttgacatatt ctaaatatcc aattaggatt tcatgataga ctgcagtcct
      901 caagcattta ggacggaaga aatgaacact atcgaggtaa gatatgtata ctctcctctg
      961 gttgggtgga gggcagtcag agccatactc ttgaacatgc atgccaaaga agcacaggtc
     1021 aacaccatca atttcttcaa aggnaagagg gctttggttc ggtatggaaa ggatnctgcc
     1081 atctctcnct gtcacaaacn tgctttcatg cctgnnttta ctncnnnntt nntcnnanca
     1141 tnactactct aacannngaa cctnnnncng acntcaggng atncnnnnnc nccctcnnaa
     1201 angtcntnnn nncnannnnn tnnnaangnn nnnannnngn nnnnnnnnnn nnncnnannn
     1261 nantttcttt nncnnnnnnn nnnnnnnnnn nnng
//
