LOCUS       sequence-459417-        1213 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of p300 KAT enzyme with truncated 
            autoinhibitory loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Dewing SM, Showalter SA
  TITLE     Probing Enzymatic Acetylation Events in Real Time With NMR 
            Spectroscopy: Insights Into Acyl-Cofactor Dependent p300 
            Modification of Histone H4.
  JOURNAL   Proteins. 2025 Jun 1. doi: 10.1002/prot.26848.
  PUBMED    40452297
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2025 Jun 1. doi: 10.1002/prot.26848."
COMMENT     Sequence Label: p300dAIL KAT LIC 1M
FEATURES             Location/Qualifiers
     source          1..1213
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      363..372
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gap             1150..1161
                     /estimated_length=12
     gap             1190..1203
                     /estimated_length=14
ORIGIN
        1 ctnctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcaagc ttgtcgacgg cgctcgaatt cggatccgtt atccacttcc aatgttatta
      121 ggtgtagaca aagcggtcct ggctctgcgt gtgcagctcc accagcatgc acatggtgga
      181 ccactgggct cttcggagtg aagagaactc caggtgcttg tcccttgcca gcgtgagaaa
      241 cgcatcccga ccatccatca gatcgcaggg gatgagagga tcaggatcaa caatgggagg
      301 cagggagttg gcagcagggc cagcaatgag gcggatcaca aagaagacct ctttatgctt
      361 ctccatggtg gcatatagtt tctgtgagag ngnnttagat acattgggca tcccgggttt
      421 cttcttgttg cccctactca ggctgctctt atttttgctg gttttctgtt ccagttcctt
      481 aatgctttct tccagaacat tgggccagaa attaccctcg aaataaggca attcctttgc
      541 acttgttaat ctatcttcag tagcttgttt aaaaatatcc ttgtagtcat ggacaatacg
      601 ctctgataca gccttgtcaa gcattttttt gtaccattcc tgcagtcgct tgggcttggg
      661 tatcttctgg tcaggaggat ggcaatggaa gatataatca tctccctcac ttggtggaca
      721 tgcccaaata tgccctgttg tgtaacctaa tttcttgaca tattctaaat atccaattag
      781 gatttcatga tagactgcag tcctcaagca tttaggacgg aagaaatgaa cactatcgag
      841 gtaagatatg tatactctnn nnnggttggg tggagggcag tcagagccat actcttgaac
      901 atgcatgcca aagaagcaca ggtcaacacc atcaatttct tcaaaggcaa agagggcttt
      961 ggttcggtat ggaaanggat tctgccatct ctcnactgtc cacaaanntt gctttcatgc
     1021 ctgnnttnac ttcnnngntt nntcananca tnantactct aacnnngacn nnnncngact
     1081 cnggnnatct nncncnncnn aanntcnttc nnnnnnnant ncnntanann nnncnannnn
     1141 nnnnangnan nnnnnnnnnn naanntnnnt ttnnntnnat nnnnnnnntn nnnnnnnnnn
     1201 nnnccnntnn nnc
//
