LOCUS       sequence-459467-       12697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-YU2gp160
FEATURES             Location/Qualifiers
     source          1..12697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    3178..3312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3202..3221)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3256..3275
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3455..3477)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4037..5826
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(6128..8053)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(8488..8506)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8574..8678
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8679..8747,8748..9539)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8897..8916)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9710..10298
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10199..10218
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10729..10874
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10917..11954
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11952..11971)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11996..12044
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat gcctatggga agtttgcaac
      661 ctctggctac gctgtatctc ttgggtatgc tggtggcctc cgtgcttgcc gcagaacaac
      721 tgtgggtcac agtatactac ggcgtccctg tgtggaaaga ggcaaccacg acgctgtttt
      781 gtgcgtccga tgcaaaggct tacgacactg aagtccataa cgtttgggcc actcacgcat
      841 gtgtccccac cgatcccaac ccccaggagg tcaaactgga aaatgtgacg gaaaatttca
      901 atatgtggaa aaataatatg gttgagcaga tgcacgagga cattatttca ctgtgggacc
      961 agtccctgaa gccttgtgtg aaattgacac ctctctgtgt cactctcaat tgcaccgatt
     1021 tgcggaatgc gacgaacact acgtccagct cttgggaaac tatggaaaaa ggagaaatta
     1081 aaaattgtag tttcaatatc accacttcca tacgggataa agttcaaaaa gagtacgccc
     1141 ttttctataa tcttgacgtc gtccctatag ataacgcaag ttacagattg ataagttgca
     1201 atacaagcgt aatcacgcag gcctgtccca aggtcagctt cgagcctata cctatacatt
     1261 attgtgcgcc tgcagggttt gcgatattga agtgtaacga caagaaattc aatggcacgg
     1321 gaccctgtac aaatgtcagc acagtccaat gcacccatgg aattcgacca gtcgtgtcaa
     1381 cccagctgct gcttaacggt agtctggcgg aggaggagat cgtgatccgg tctgagaact
     1441 tcactaataa cgctaagacc atcatagtcc agcttaatga gagtgtggtg atcaactgca
     1501 ctaggcccaa taacaatacg cgcaagtcca ttaacattgg ccctgggcgg gccctttata
     1561 caacgggcga aattattggg gatatccgac aggcccactg taatctgtcc aaaactcagt
     1621 gggagaatac acttgagcag attgccatca aacttaagga acaatttggc aacaataaga
     1681 caatcatttt caatcctagc tcaggaggcg acccagaaat tgttacccat tcatttaatt
     1741 gcggcgggga attcttctat tgcaattcaa cccagctttt tacctggaat gatactcgca
     1801 agctgaacaa caccggcagg aacataactc tgccttgcag gataaagcag ataataaata
     1861 tgtggcaaga agtaggcaaa gcgatgtatg caccgcccat ccggggacaa attcgatgca
     1921 gctcaaacat tactggtttg ttgttgacgc gggatggcgg caaggacacc aacggcacgg
     1981 agatttttag gcccggcggc ggtgatatga gagataattg gcggtcagag ctgtataagt
     2041 acaaggtggt gaagattgaa cccctgggcg tggcaccaac gaaagcaaaa agaagggttg
     2101 tacaacgcga gaaacgcgcc gttgggctcg gcgcgctctt cctcggattc ttgggagcag
     2161 ccggtagtac gatgggggcc gcctcaataa cgctgacggt ccaggcgagg caactcctct
     2221 ctggaatagt acaacagcag aataacctcc tgagggcaat agaggctcag caacatctgt
     2281 tgcaacttac tgtctggggc atcaaacaac tgcaggccag agtgcttgcc gtggagagat
     2341 atttgcgcga ccaacaactc ctgggtatct gggggtgctc cggtaagctt atctgcacga
     2401 caacggtacc gtggaatacc tcatggtcta ataagtcact taatgaaatc tgggataata
     2461 tgacgtggat gaagtgggag cgagagattg acaactatac acatattata tattcactga
     2521 ttgaacagtc ccaaaatcaa caggaaaaaa acgaacagga actcctcgca ctcgataaat
     2581 gggcttctct gtggaattgg ttcgacataa cgaagtggtt gtggtatatt aaaatattca
     2641 taatgatcgt cggagggttg atcggcctcc gaattgtctt tgttgtgctc agtatcgtaa
     2701 accgcgtacg ccagggttat agtcctttgt catttcagac acacctcccc gcccaacggg
     2761 gcccagaccg gcccgacggc atcgaagagg aggggggtga gagagaccgc gacaggtctg
     2821 gcccgcttgt tgatggattc cttgcaatca tatgggtcga tttgcgcagc ttgtgtcttt
     2881 tttcttatca caggctgcga gacctccttt tgatagtaac ccggattgtt gaattgttgg
     2941 gtagacgcgg ttggggcgtg ctgaagtact ggtggaattt gcttcaatat tggatccaag
     3001 agctgaaaaa ttccgccgtt agcctcctca atgctacggc gatagcagtt gccgaaggca
     3061 cggatcgcgt aatagaaatc ctgcagcgag cttttcgggc ggtccttcat atccctgtgc
     3121 ggatccgcca ggggcttgag cgcgcactgc tttagtaact cgaggccggc aaggccggat
     3181 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3241 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3301 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     3361 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     3421 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     3481 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     3541 cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc
     3601 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     3661 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     3721 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     3781 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     3841 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     3901 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     3961 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     4021 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     4081 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     4141 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     4201 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     4261 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     4321 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     4381 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     4441 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     4501 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     4561 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     4621 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     4681 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     4741 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     4801 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     4861 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     4921 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     4981 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     5041 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     5101 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     5161 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     5221 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     5281 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     5341 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     5401 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     5461 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5521 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5581 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     5641 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     5701 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     5761 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     5821 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     5881 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     5941 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     6001 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     6061 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     6121 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     6181 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     6241 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     6301 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     6361 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     6421 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     6481 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     6541 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     6601 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     6661 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     6721 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     6781 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     6841 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     6901 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     6961 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     7021 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     7081 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     7141 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     7201 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     7261 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     7321 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgcccctc
     7381 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc
     7441 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc
     7501 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc
     7561 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc
     7621 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
     7681 ctgcccctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg
     7741 ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg
     7801 tccctttgca gccaatgcaa cttggacgtt tttggggtct ccggacacca tctctatgtc
     7861 ttggccctga tcctgagccg cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc
     7921 ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg aggtccactg ccgccggagc
     7981 cttctggtcc agatgtgtct cccttctctc ctaggccatt tccaggtcct gtacctggcc
     8041 cctcgtcaga catgattcac actaaaagag atcaatagac atctttatta gacgacgctc
     8101 agtgaataca gggagtgcag actcctgccc cctccaacag cccccccacc ctcatcccct
     8161 tcatggtcgc tgtcagacag atccaggtct gaaaattccc catcctccga accatcctcg
     8221 tcctcatcac caattactcg cagcccggaa aactcccgct gaacatcctc aagatttgcg
     8281 tcctgagcct caagccaggc ctcaaattcc tcgtccccct ttttgctgga cggtagggat
     8341 ggggattctc gggacccctc ctcttcctct tcaaggtcac cagacagaga tgctactggg
     8401 gcaacggaag aaaagctggg tgcggcctgt gaggatcagc ttatcgatga taagctgtca
     8461 aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     8521 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8581 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8641 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8701 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8761 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8821 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8881 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     8941 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9001 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9061 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9121 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9181 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     9241 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9301 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9361 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9421 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9481 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9541 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9601 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9661 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9721 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9781 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9841 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9901 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9961 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10021 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10081 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10141 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10201 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10261 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10321 tacggttcct ggccttttgc tggccttgaa gctgtccctg atggtcgtca tctacctgcc
    10381 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
    10441 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg
    10501 ccccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca
    10561 agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt
    10621 ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg
    10681 ctggcggtgt ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc
    10741 ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt
    10801 ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc
    10861 agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa tgagatatga
    10921 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
    10981 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
    11041 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
    11101 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
    11161 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
    11221 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
    11281 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
    11341 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
    11401 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
    11461 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
    11521 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
    11581 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
    11641 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
    11701 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
    11761 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
    11821 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
    11881 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccggat cgggagatgg
    11941 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
    12001 ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg
    12061 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
    12121 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
    12181 caacgtcggg gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc
    12241 cccatgggga atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtcag
    12301 gtccacgact ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc
    12361 atgtactggc gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa
    12421 aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac caattctcat gtttgacagc
    12481 ttatcatcgc agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta
    12541 tggaagatct atacattgaa tcaatattgg caattagcca tattagtcat tggttatata
    12601 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac
    12661 atttatattg gctcatgtcc aatatgaccg ccatgtt
//
