LOCUS       sequence-459476-        5090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE1426m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in striatal Sst-Chodl interneurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP15571: pAAV-AiE1426m-minBG-iCre(R297T)-BGHp...
FEATURES             Location/Qualifiers
     source          1..5090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      1026..1035
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1038..1058
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2124..2235
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2353..2493
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2353..2482
                     /label=AAV2 ITR
     rep_origin      2568..3023
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2655..2674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2865..2886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3040..3059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3159..3181
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3219..3237)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3305..3409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3410..3478,3479..4270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3628..3647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4441..5029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4930..4949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtattatg ctaatatgcc ataattaaca taaattaaca
      181 tagttctttg tgggacccca gttaattcat gtgagaaaac ataaaatgtc agggtcgcgg
      241 cgaggggccg gtaggggtgg gcggaggttt ctgaggaggg agcttttggg agcttcgagt
      301 caacaataaa ggaccgaggg ttcggagcag gagtggcctc ggtaaatgaa ttcagatata
      361 attcaagctt attagagggc tttttaaaaa aataaaaaat tgattcagtg tatgaaagca
      421 agttaactac ttctgttcac cattgagaga cttacaggtg cttgcctgaa ctgcaataaa
      481 ggactcttat tattgatcag gacttattta tctcttcatt ttggagagcc tcataaactg
      541 ttattacagt ttctctactg actttgaaaa gtttgaagtt tgaaagagac acctgtgcag
      601 ctaacaaagc atgagcacgg ccagaacaga gaaccctgtt atcatgggtc tatccagtca
      661 gaacggccag ctgaggggcc ctgtgaaagc cagtgctggc cctggaggag ggggcacaca
      721 gccacagatg aaccagttga agaacaccaa cacaatcaat aacggcactc cgcagcaagc
      781 ccagagcatg gccgccacga ttaagcatgt ctgatgttca taaaggccag aagagtttgt
      841 tgaagccact gaacttaggt tataaatgat gacctggtga tgactggaaa gacattaaaa
      901 ctccctccaa aggatctaag aatcaaaact ctggatgtga cctcgagctc gggctgggca
      961 taaaagtcag ggcagagcca tctattgctt acatttgctt ctgggatcca gatctttcga
     1021 agctagccac catggtgccc aagaagaaga ggaaagtctc caacctgctg actgtgcacc
     1081 aaaacctgcc tgccctccct gtggatgcca cctctgatga agtcaggaag aacctgatgg
     1141 acatgttcag ggacaggcag gccttctctg aacacacctg gaagatgctc ctgtctgtgt
     1201 gcagatcctg ggctgcctgg tgcaagctga acaacaggaa atggttccct gctgaacctg
     1261 aggatgtgag ggactacctc ctgtacctgc aagccagagg cctggctgtg aagaccatcc
     1321 aacagcacct gggccagctc aacatgctgc acaggagatc tggcctgcct cgcccttctg
     1381 actccaatgc tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg
     1441 agagagccaa gcaggccctg gcctttgaac gcactgactt tgaccaagtc agatccctga
     1501 tggagaactc tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca
     1561 acaccctgct gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg
     1621 atggtgggag aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg
     1681 tggagaaggc cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg
     1741 gtgtggctga tgaccccaac aactacctgt tctgccgggt cagaaagaat ggtgtggctg
     1801 ccccttctgc cacctcccaa ctgtccaccc gggccctgga agggatcttt gaggccaccc
     1861 accgcctgat ctatggtgcc aaggatgact ctgggcagag atacctggcc tggtctggcc
     1921 actctgccag agtgggtgct gccaccgaca tggccagggc tggtgtgtcc atccctgaaa
     1981 tcatgcaggc tggtggctgg accaatgtga acattgtgat gaactacatc agaaacctgg
     2041 actctgagac tggggccatg gtgaggctgc tcgaagatgg ggactgaggc gcgccgaatt
     2101 caagcttctc gagagatctt cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     2161 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     2221 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     2281 gcaggacagc aagggggagg attgggaaga caatagcagg catgagatct cacgtgcgga
     2341 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     2401 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     2461 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     2521 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     2581 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2641 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2701 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     2761 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     2821 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     2881 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     2941 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3001 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     3061 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     3121 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     3181 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     3241 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3301 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3361 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3421 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3481 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3541 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3601 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3661 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3721 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3781 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3841 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3901 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3961 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4021 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4081 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4141 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4201 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4261 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4321 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4381 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4441 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4501 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4561 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4621 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4681 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4741 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4801 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4861 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4921 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4981 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5041 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
