LOCUS       sequence-459526-       10872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for F-actin labeling in living cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W
  TITLE     Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated
            GLUT4 exocytosis through cortical actin remodeling.
  JOURNAL   Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.
  PUBMED    25575350
REFERENCE   2  (bases 1 to 10872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-01-9"; volume: "6:5951. doi"; pages: " 
            10.1038/ncomms6951"
COMMENT     Sequence Label: pLenti-Lifeact-tdTomato
FEATURES             Location/Qualifiers
     source          1..10872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    258..491
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             676..720
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1018..1135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1200..2411
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2248..2267
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             2501..2551
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      2558..2567
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2564..3994
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    4142..4730
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4195..4215)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4613..4624)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4733..4749)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4734..4750)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5255..5435
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5461..5478)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5467..5691
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5737..6165
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5824..5843)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6034..6055
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6174..6194)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6179..6508
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6359..6494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6421..6440
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6556..6603
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6622..6996
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7126..7247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7163..7182)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7217..7236
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7296..7312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7296..7312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7309..7331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7320..7336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7344..7350,7351..7368,7369..7374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7389..7410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7527..7544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7698..8286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7778..7797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8457..9248,9249..9317))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9080..9099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9318..9422)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9497..9516)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9688..10067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10069..10267
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10222..10242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10285..10465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10512..10637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
       61 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
      121 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
      181 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
      241 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
      301 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
      361 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
      421 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
      481 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
      541 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
      601 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
      661 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
      721 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
      781 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
      841 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
      901 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
      961 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     1021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     1141 ttattacagg gacagcagag atccagtttg gttaattaac ccgtgtcggc tccagatctg
     1201 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc
     1261 cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc
     1321 ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg acattttagg
     1381 acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga
     1441 aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg
     1501 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc
     1561 gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg
     1621 gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc
     1681 aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc
     1741 agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag
     1801 gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc
     1861 gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg
     1921 acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt
     1981 atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg
     2041 tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc
     2101 ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc
     2161 gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg
     2221 ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt
     2281 tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa
     2341 tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt
     2401 ttttgttaga cgaagcttgg gctgcaggtc gactctagag gatcgaattc ttcgaagcta
     2461 gcgctaccgg actcagatct cgagctcaag cttcgaattc atgggcgtgg ccgacctgat
     2521 caagaagttc gagagcatca gcaaggagga gccggtcgcc accatggtga gcaagggcga
     2581 ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     2641 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     2701 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     2761 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     2821 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     2881 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     2941 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     3001 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     3061 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa
     3121 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     3181 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     3241 ggggcatggc accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa
     3301 caacatggcc gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa
     3361 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     3421 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     3481 ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa
     3541 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     3601 tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa
     3661 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
     3721 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca
     3781 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc
     3841 caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc
     3901 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
     3961 gttcctgtac ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca
     4021 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     4081 acctgaaaca taaaatgaat gcaattgttg ttgttaacaa ttcgatatca agcttatcga
     4141 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4201 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4261 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4321 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4381 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4441 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4501 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     4561 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     4621 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     4681 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     4741 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     4801 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     4861 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4921 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     4981 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     5041 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     5101 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     5161 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     5221 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     5281 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5341 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5401 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     5461 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5521 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5581 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5641 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5701 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     5761 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5821 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5881 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5941 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6001 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6061 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6121 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6181 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6241 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6301 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6361 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6421 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     6481 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     6541 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     6601 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     6661 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     6721 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     6781 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     6841 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     6901 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     6961 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     7021 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     7081 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     7141 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7201 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     7261 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7321 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7381 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7441 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7501 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7561 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7621 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7681 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7741 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7801 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7861 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7921 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7981 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8041 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8101 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8161 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8221 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8281 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8341 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8401 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8461 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8521 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8581 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8641 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8701 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8761 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8821 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8881 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8941 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9001 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9061 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9121 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9181 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9241 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9301 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9361 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9421 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     9481 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     9541 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     9601 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     9661 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     9721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     9781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     9841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     9901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     9961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
    10021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
    10081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
    10141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
    10201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
    10261 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
    10321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    10381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
    10501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
    10561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    10621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
    10681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
    10741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    10801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    10861 atacagtagc aa
//
