LOCUS       sequence-459548-       11503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing 
            construct into the C. neoformans genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11503)
  TITLE     C. neoformans AID2 degron system plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBHM2643
FEATURES             Location/Qualifiers
     source          1..11503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1963..1982
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1963..1981
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2799..3599
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2859..2878)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3469..3488
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             6820..6873
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      7129..7138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7603..7806
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     primer_bind     complement(8685..8701)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8686..8702)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(8730..8748)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8769..8785)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8769..8785)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8782..8804)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8793..8809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8817..8823,8824..8841,8842..8847))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8862..8883
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9000..9017)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9171..9759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9251..9270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9930..10721,10722..10790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10553..10572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10791..10895)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    10932..11435
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     11476..11494
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     1981 ggcgaattgg agctccaccg cggtggcggc cgctctagaa ctagtgcatg caggattcga
     2041 gtggcatggt gtgcactgag tgtatggttg tcggaggaga ggatgatggt aacaacaata
     2101 gcagcaacgt cactcgacgc gcgtccggtg tgccacacgg ggtaacgccg agtcgccgtc
     2161 agggtcgccg agaccactct cacagcgtca ccgttggcac cagctcagct tacagcttct
     2221 atcctccgcc agcatccaca tacatcccct ataccgcatc ccccacccac tgcccaaggt
     2281 gagtcatctt cccgccccct tcccttgccc gccactcagt cctccatcct ccactaatcc
     2341 accttatcgc acccaccgcc tatcgcacat ccgagcacaa tgctgggcct gccaggggct
     2401 gctagatggt gctctcccca cgctgatctg catgccggcc attggatcat gggtgctagg
     2461 tgctgggtgc tggatgttgg atgctggatg ctgggtgcac gcttggtcat ttccttccag
     2521 gattgacggt cgccgagagg acgacgtggc gttcgacaac gagggccgat agcaccgcat
     2581 cgcctcgacc tgcatccatc tcgccttgtc cttttggtgc aacaatccat ccgtgctggt
     2641 gccacacgca tagctggaag agatggatgt gcgttgaaca gagctgccgt caggactttt
     2701 tggtgcacgg accctattgt cctccccaat cttcaccgcg tctcctaata tgcagcctct
     2761 ttgctaattg tctttttcca ttagtaaact cgcccaacat gtctatgatt gaacaagatg
     2821 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     2881 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     2941 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     3001 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     3061 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatccc
     3121 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     3181 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     3241 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     3301 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     3361 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     3421 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     3481 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     3541 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     3601 tgaaggcggt aaggggttaa ttttccttag agggtgtata tatacatatt agagaagtga
     3661 tacaatttta gcacactgcg aattcgagac agacatcgtg tcaatcatct tttttgacat
     3721 tatatgccca ttaatctatc tacagacaac aataccatcc ttcccaccct cagcaacgcc
     3781 gttgaatcct caggatcttc atggccattc tatgctatac ggtacaagat tgtggctact
     3841 atgcaagctc catagcgaat gatcttacac tgaccttgtc gaccgttctg aatgattaac
     3901 aatggtcgca agtgtggttg acccacctgc gggcgcttct taactatcta tcatcggtat
     3961 gtcaagtatc actcccttta aacaaaagaa taagagcata atacagcagt ctcatcatgg
     4021 caacccttcg cgcaatgtac aaaaagcagc cagaagcccc cacgcacaag caataatgtc
     4081 gtgttctacc ctgccatgaa caagcgtcat ggccattagc gaacgcccca atattcagcc
     4141 ccattcgcca agcaagttgc ctcgccccct tcccagaaga tcatgggcat ctcgtcttct
     4201 tctaatttcg cattttgtga agctccattc atgcacgtcg gcttctggac gactcttatc
     4261 atatgcattg gcctgctcgg tgtgaggatg agccactccc acatgtttag acggcagggg
     4321 cctcttgcca cccgttgaat ttttggtgcg aagacgtgcc tgggaacgac ctccacttta
     4381 aatttctccg ttgttgcggt cgctccatcc tcctcacatt tcttgcctcc taagtcccat
     4441 gtgccacact cggtgaaaaa cggagtttga tacggatgaa atttccagtg agcttttcac
     4501 attctggagg tttcgaaaaa aattgtggga ttttctgaat taatccgaga atccgccctg
     4561 cccatcaacc ggaaagaatt aaatccctcg cacattcctt tctcccacca ccttctttat
     4621 ctctacattc tttaaccaaa acacctaggt gagtacccaa agtcttttga cacctttgaa
     4681 aatccgcgcc agtcgtaggg cacgtaaaac aattctcgct ggtggcttct gcaggtgaag
     4741 tcggggcaga gtggagctga gcatggtcga ccacccacat cattctgtct tgtttttggc
     4801 gatgtcttcc atgcttttgc cctcatctgt tgcgtcgact gcagagtact gttttgccac
     4861 tactgagctc aacatgggct tgtgtgtggt gaagaaaaaa atttggtggc tcttgaaaat
     4921 tttcaaccca tcggatacat gcccttccaa ctcacaacgc cctaacactt tattgtccta
     4981 tcattgcctc ttcgcacatg ctaacgatct ccacagaaaa cttcaaaatg acctacttcc
     5041 ccgaggaggt cgtcgagcac atcttctcct tcctccccgc ccagcgagac cgaaacaccg
     5101 tctccctcgt ctgtaaggtc tggtacgaga tcgagcgact ctcccgacga ggtgtcttcg
     5161 tcggtaactg ttacgccgtc cgagccggtc gagtcgccgc ccgattcccc aacgtccgag
     5221 ccctcaccgt caagggtaag ccccacggtg ccgacttcaa cctcgtcccc cccgactggg
     5281 gtggttacgc cggtccctgg atcgaggccg ccgcccgagg ttgtcacggt ctcgaggagc
     5341 tccgaatgaa gcgaatggtc gtctccgacg agtccctcga gctcctcgcc cgatccttcc
     5401 cccgattccg agccctcgtc ctcatctcct gtgagggttt ctccaccgac ggtctcgccg
     5461 ccgtcgcctc cgtatgtaac cccgtttccc gcaacatgtt actccactaa catctcccga
     5521 cagcactgta agctcctccg agagctcgac ctccaggaga acgaggtcga ggaccgaggt
     5581 ccccgatggc tctcctgttt ccccgactcc tgtacctccc tcgtctccct caacttcgcc
     5641 tgtatcaagg gtgaggtcaa cgccggttcc ctcgagcgac tcgtctcccg atcccccaac
     5701 ctccgatccc tccgactcaa ccgatccgtc tccgtcgaca ccctcgccaa gatcctcctc
     5761 cgaaccccca acctcgagga cctcggtacc ggtaacctca ccgacgactt ccagaccgag
     5821 tcctacttca agctcacctc cgccctcgag aagtgtaaga tgctccgatc cctctccggt
     5881 ttctgggacg cctcccccgt ctgtctctcc ttcatctacc ccctctgtgc ccagctcacc
     5941 ggtctcaacc tctcctacgc ccccaccctc gacgcctccg acctcaccaa gatgatctcc
     6001 cgatgtgtca agctccagcg actctgggtc ctcgactgta tctccgacaa gggtctccag
     6061 gtcgtcgcct cctcctgtaa ggacctccag gagctccgag tcttcccctc cgacttctac
     6121 gtcgccggtt actccgccgt caccgaggag ggtctcgtcg ccgtctccct cggttgtccc
     6181 aagctcaact ccctcctcta cttctgtcac cagatgacca acgccgccct cgtcaccgtc
     6241 gccaagaact gtcccaactt cacccgattc cgactctgta tcctcgagcc cggtaagccc
     6301 gacgtcgtca cctcccagcc cctcgacgag ggtttcggtg ccatcgtccg agagtgtaag
     6361 ggtctccagc gactctccat ctccggtctc ctcaccgaca aggtcttcat gtacatcggt
     6421 aagtacgcca agcagctcga gatgctctcc atcgccttcg ccggtgactc cgacaagggt
     6481 atgatgcacg tcatgaacgg ttgtaagaac ctccgaaagc tcgagatccg agactccccc
     6541 ttcggtgacg ccgccctcct cggtaacttc gcccgatacg agaccatgcg atccctctgg
     6601 atgtcctcct gtaacgtcac cctcaagggt tgtcaggtcc tcgcctccaa gatgcccatg
     6661 ctcaacgtcg aggtcatcaa cgagcgagac ggttccaacg agatggagga gaaccacggt
     6721 gacctcccca aggtcgagaa gctctacgtc taccgaacca ccgccggtgc ccgagacgac
     6781 gcccccaact tcgtcaagat cctcagatca ggaagcggag agggtcgagg ttccctcctc
     6841 acctgtggtg acgtcgagga gaaccccggt cccatggtct ccaagggtga ggaggacaac
     6901 atggcctccc tccccgccac ccacgagctc cacatcttcg gttccatcaa cggtgtcgac
     6961 ttcgacatgg tcggtcaggg taccggtaac cccaacgacg gttacgagga gctcaacctc
     7021 aagtccacca agggtgacct ccagttctcc ccctggatcc tcgtccccca catcggttac
     7081 ggtttccacc agtacctccc ctaccccgac ggtatgtccc ccttccaggc cgccatggtc
     7141 gacggttccg gttaccaggt ccaccgaacc atgcagttcg aggacggtgc ctccctcacc
     7201 gtcaactacc gatacaccta cgagggttcc cacatcaagg gtgaggccca ggtcaagggt
     7261 accggtttcc ccgccgacgg tcccgtcatg accaactccc tcaccgccgc cgactggtgt
     7321 cgatccaaga agacctaccc caacgacaag accatcatct ccaccttcaa gtggtcctac
     7381 accaccggta acggtaagcg ataccgatcc accgcccgaa ccacctacac cttcgccaag
     7441 cccatggccg ccaactacct caagaaccag cccatgtacg tcttccgaaa gaccgagctc
     7501 aagcactcca agaccgagct caacttcaag gagtggcaga aggccttcac tgacgtcatg
     7561 ggtatggacg agctctacaa gggttctgga tccggaggta gtaaggagaa gtccgcctgt
     7621 cccaaggacc ccgccaagcc ccccgccaag gcccaggtcg tcggttggcc ccccgtccga
     7681 tcctaccgaa agaacgtcat ggtctcctgt cagaagtcct ccggtggtcc cgaggccgcc
     7741 gccttcgtca aggtctccat ggacggtgcc ccctacctcc gaaagatcga cctccgaatg
     7801 tacaagtaat tacttatgta gtgacccttc ttacgcgtaa tatgcatcgg atgactgttg
     7861 ctacttgtgc ttcttacgtt ttgtgtctta tttgctgatg tatacctctg cgcttcaaaa
     7921 tcttcattgc cgtgatatat cattccatgt cgtttgtccg ttattggatt cccgatagtt
     7981 gtccgcgaaa tcgaatattt ctcggctgac gacgtctctt gcaacaataa aatggccatc
     8041 actgttgaaa tttgcacgag agctataact tggaataacc ttcgttgctg ctttatttta
     8101 gtgctttcaa ctatttactc attctcaggt cgccaccatc gcattgatat cgttccttcc
     8161 atccttcctt tcctttagtc ggtcacgtct tccttaatcc gtcactcctt ttttcagtcg
     8221 ttcctggaga tcaccacgaa ataatctcga catgcactga ccatctcggt tctgtggact
     8281 gtcatgctaa gagagttcat gttgtaataa tagtttgttg catggaaaga acggggaaag
     8341 aagcaagaaa cagatgtaca gaattaccga tgtctctacg catccctgac ctcgtccttc
     8401 ctaccgcaac cgcacttcca cctgctcaca cgtcctcgtt aatgccttga atctcagcca
     8461 cacacttacc tcgcctcgac gcttgtcggg ccagggcacc cccttcatca acctgggaag
     8521 agttggcggg gcaaacggag gagggatggg gcaggaagag ggggatggcg cacgtcgagt
     8581 ggatttggaa gaaaaggggt tggtcgaggt ggaagtcgaa gcgaagatgt tgaatgtcat
     8641 gtttgggaac gggggatctt tagaagacta tgagattgaa cgatcgatac cgtcgacctc
     8701 gagggggggc ccggtaccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
     8761 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8821 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8881 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8941 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9001 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9061 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9121 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9361 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9601 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10921 gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta atataaatat
    10981 ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt tgttttccga
    11041 agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt gctcttcctg
    11101 ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca cacacgaaaa
    11161 tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta atctgctttt
    11221 cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt
    11281 tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa
    11341 atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa
    11401 gatacgaggc gcgtgtaagt tacaggcaag cgatccgtct aagaaaccat tattatcatg
    11461 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
