LOCUS       sequence-459556-        6394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An AAV genome that expresses GFP from the mouse TH promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Chan KY, Jang MJ, Yoo BB, Greenbaum A, Ravi N, Wu WL, 
            Sanchez-Guardado L, Lois C, Mazmanian SK, Deverman BE, Gradinaru V
  TITLE     Engineered AAVs for efficient noninvasive gene delivery to the 
            central and peripheral nervous systems.
  JOURNAL   Nat Neurosci. 2017 Aug;20(8):1172-1179. doi: 10.1038/nn.4593. Epub 
            2017 Jun 26.
  PUBMED    28671695
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.4593"; 
            journalName: "Nat Neurosci"; date: "2017-08"; volume: "20"; issue: 
            "8"; pages: "1172-1179"
COMMENT     Sequence Label: pAAV-mTH-GFP
FEATURES             Location/Qualifiers
     source          1..6394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      3411..3420
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3417..3419,3420..3422,3423..4136)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3417..3419,3420..3422,3423..4133)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3462..3483)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3723..3742)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4070..4091
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4196..4216)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4614..4625)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4755..4876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4766..4785)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4820..4839
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4979..5108
                     /label=AAV2 ITR
     primer_bind     complement(5173..5191)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5259..5363
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5364..5432,5433..6224)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5582..5601)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt aataaacaca
      661 cacacaccaa caaccgtggt tggttgttgt gttggtttat tctcgagttg gccactccct
      721 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccgg cctcagtgag
      781 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct actagttaag
      841 gggcctcttg gagttgttct catcaaatgg ggatgggaca cagctaaagt gcccagggct
      901 tctctgtgcc cacagatgct ttagatcttg gcacagtgtg gtctaccagc tgtctctctc
      961 tgtgtatata tatgtatttc atagacagtg tacagtggcc tggtttgtgc tatcaggctg
     1021 gatatggaca gaggcaagag tttgtggcag cagttatctc ccaagagagt ccaaagacat
     1081 catgttttca agtttaggcc aggtgctact tgagagagct cagacacaga caaaggtctg
     1141 gagagcacat gtcctccacc cccacctagc ttctgttgca agcacctcca gccgagacaa
     1201 gagaacgaat taaaaagcaa tatttgtgtc agtgtaagac atttgccgaa aggttaaatc
     1261 cacattcgtg ttgctgcaga gcagccccct atgcaggatt tgttagatac agctccgtcc
     1321 taccctgtgc cagctgagca aacgccaggc tgggtggggt ggaacccagc ctgggtttgc
     1381 ctcaccctgc aatcccccca gcaccctcta aaggaggacc ctgtggtggg catgcagacc
     1441 tagggactgg gcatagataa cctttgggtt tgggcaacag cccccactcc tcaggattga
     1501 aggctaaggt gcagccagct ctgccttcat ggtgggaatg tctccacgtg acccctttct
     1561 gggctgtgga gaacactcag agaagagtcc tgggatgcca ggcaggccag ggatgtgctg
     1621 ggcatgttga gacaggagtg ggctaagcca gcagagttgc tgacccagga agagttcaga
     1681 aaggggcatg gaacatgggg aggggtccat agtgagagag agcaggcagt gcagagtaaa
     1741 tagtccctga gctgggggtt atgggatttg caggagcttg ctcagagaag gcagaggaga
     1801 gatgctgcgc caagctgggt atcacagagc ctcagactcc tggaacagga actgtggggg
     1861 tcaggtcagc aggggaggtt agggagtgtt ccctttgtac tgacttagca tttatcctgc
     1921 ttctaggggg gaaggggggc cagtggggga tgcacagcaa ggcagtgatg tggcaggcag
     1981 cctgcgggag ctcctggttc ctggtgtgaa aaagctggga aggaagaggg ctgggtctgg
     2041 taagtacagc aggcagttgg ctcctgagag tccaagccct gtctagaggg tggagtgaga
     2101 tttcagaggg agagctaaac ggggtggggg ctggggagtc caggcttctg gctcctgcta
     2161 atactcagtg tgctgggtcc tcagaacctc agggtggcca ttttcagggt gagagctctg
     2221 tcctttggca cttctgcaga ctccagtatc cagaggaata aagatggtac tcttcctcag
     2281 ttcccttagt gagaggacac ctttctctga agggcttggg cagttgtcct gaaccattgc
     2341 ctgaaggaag gacttgactc cagggacata gaatgggctc agcataagtc ccctgtagta
     2401 gagaaaggtc ccctctctgg tctccttaga gatcctgttt ccttggctga ggaagctagg
     2461 gtggatcttt gtgtaagtgg gtgtggatgc tcactggaaa tcaaaaggcc ccttggtgtt
     2521 agaccttggg gtgccatggg agagttgatc actgagtgcg cccttacatg ggggccagct
     2581 gagaatgggg ctgcctctag ctcgagacca tgatgcaggg agtgagtggg ggagttcagg
     2641 atactcttaa ctaaagcaga ggtctgtccc cccagggagg ggaggtcaga agaccctagg
     2701 gagatgccaa aggctagggt tggcaccatg ttgcaggctg tgtcttcaag gagatgataa
     2761 tcagaggaat cgaacctgca aaagtgggcc agtcttagat acactataga ggaataatct
     2821 tctgaaacat tctgtgtctc ataggacctg cctgaggacc cagccccagt gccagcacat
     2881 acactggggc agtgagtaga tagtatactt tgttacatgg gctgggggga catggcctgt
     2941 gccctggagg ggacttgaag acatccaaaa agctagtgag agggctccta gatttatttg
     3001 tctccaaggg ctatatatag ccttcctaac atgaaccctt gggtaatcca gcatgggcgc
     3061 tcccatatgc cctggtttga ttagagagct ctagatgtct cctgtcccag aacaccagcc
     3121 agcccctgtc ttcatgtcgt gtctagggcg gagggtgatt cagaggcagg tgcctgcgac
     3181 agtggatgca attagatcta atgggacgga ggcctctctc gtccgtcgcc ctcgctctgt
     3241 gcccaccccc gcctccctca ggcacagcag gcgtggagag gatgcgcagg aggtaggagg
     3301 tgggggaccc agaggggctt tgacgtcagc ctggccttta agaggccgcc tgcctggcaa
     3361 gggctgtgga gacagaactc gggaccacca gcttgcacta tgcccacgcg gccgccatgg
     3421 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3481 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3541 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3601 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3661 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3721 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3781 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3841 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     3901 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3961 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4021 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4081 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaggat
     4141 cctaataacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4201 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4261 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4321 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4381 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4441 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4501 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     4561 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     4621 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4681 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct
     4741 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4801 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4861 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     4921 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcaagcttag
     4981 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     5041 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     5101 gcgcgcagag agggagtggc caagctagcg ggcgattaag gaaagggcta gatcattctt
     5161 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5221 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5281 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5341 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5581 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     5641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6361 cgtcagaccc cgtagaaaag atcaaaggat cttc
//
